ENZYME     RECOGNITION SEQ.     CUTTING POSITION(S)     7/15/88

     Aat1       AGGCCT                3
     Aat2       GACGTC                5
     Acc1       GTMKAC                2
     Aha2       GRCGYC                2
     Aoc1       CCTNAGG               2
     Apa1       GGGCCC                5
     ApaL1      GTGCAC                1
     Asu2       TTCGAA                2
     Asp718     GGTACC                1
     Ava1       CYCGRG                1
     Avi2       TGCGCA                3
     Avr2       CCTAGG                1
     Bal1       TGGCCA                3
     BamH1      GGATCC                1
     Ban1       GGYRCC                1
     Ban2       GRGCYC                5
     Ban3       ATCGAT                2
     Bcl1       TGATCA                1
     Bgl1       GCCNNNNNGGC           7
     Bgl2       AGATCT                1
     Bsm1       GAATGC                7    5
     BspM1      ACCTGC               10   14
     BspM2      TCCGGA                1
     Bsp1286    GDGCHC                5
     BssH1      GCGCGC                1
     BstE2      GGTNACC               1
     BstX1      CCANNNNNNTGG          8
     Cel2       GCTNAGC               2
     Cfr10I     RCCGGY                1
     Cla1       ATCGAT                2 
     Dra1       TTTAAA                3
     Dra3       CACNNNGTG             6
     Eae1       YGGCCR                1
     Eag1       CGGCCG                1
     Eco47III   AGCGCT                3
     EcoO109    RGGNCCY               2
     EcoR1      GAATTC                1
     EcoR5      GATATC                3
     Esp1       GCTNAGC               2
     Fsp1       TGCGCA                3
     Hae2       RGCGCY                5
     HgiA1      GWGCWC                5
     Hinc2      GTYRAC                3
     Hind3      AAGCTT                1
     Hpa1       GTTAAC                3
     Kpn1       GGTACC                5
     Mlu1       ACGCGT                1
     Mst2       CCTNAGG               2
     Nae1       GCCGGC                3
     Nar1       GGCGCC                2
     Nco1       CCATGG                2
     Nde1       CATATG                2
     Nhe1       GCTAGC                1
     Not1       GCGGCCGC              2
     Nru1       TCGCGA                3
     Nsi1       ATGCAT                5
     Nsp2       GDGCHC                5
     Nsp3       CYCGRG                1
     PaeR71     CTCGAG                1
     PpuM1      RGGWCCY               2
     Pss1       RGGNCCY               0
     Pst1       CTGCAG                5
     Pvu1       CGATCG                4
     Pvu2       CAGCTG                3
     Rsp1       CGATCG                4
     Rsr2       CGGWCCG               2
     Sac1       GAGCTC                5
     Sac2       CCGCGG                4
     Sal1       GTCGAC                1
     Sca1       AGTACT                3
     Sfi1       GGCCNNNNNGGCC         8
     Sma1       CCCGGG                3
     SnaB1      TACGTA                3
     Spe1       ACTAGT                1
     Sph1       GCATGC                5
     Ssp1       AATATT                3
     Sst1       GAGCTC                5
     Sst2       CCGCGG                4
     Stu1       AGGCCT                3
     Sty1       CCWWGG                1
     Tth111-1   GACNNNGTC             4
     Xba1       TCTAGA                1
     Xho1       CTCGAG                1
     Xho2       RGATCY                1
     Xma1       CCCGGG                1
     Xma3       CGGCCG                1
     Xmn1       GAANNNNTTC            5
     Xor2       CGATCG                4