
salt conc. = 50.00 mM   DNA conc. = 50.00 nM

                                           |Self   |--B1-- --B2-- --L1--|
? base primer 5'->3'              Tm   GC% |any 3' |any 3' any 3' any 3'|
>    1 TNNAGCTCGGTACCCGGG        63.7  69%
>    2 NNAGCTCGGTACCCGGGG        67.2  75%
>    3 NAGCTCGGTACCCGGGGA        68.8  71%
>    4 AGCTCGGTACCCGGGGAT        64.0  67%
>    5 GCTCGGTACCCGGGGATC        65.0  72%
>    6 CTCGGTACCCGGGGATCC        64.9  72%
>    7 TCGGTACCCGGGGATCCT        64.9  67%
>    8 CGGTACCCGGGGATCCTA        62.9  67%
>    9 GGTACCCGGGGATCCTAA        59.6  61%
>   10 GTACCCGGGGATCCTAATAG      57.4  55% | 6  5  | 5  4   5  4  10  9 | ok
>   11 TACCCGGGGATCCTAATAG       56.5  53% | 6  5  | 5  4   5  4  10  9 | ok
>   12 ACCCGGGGATCCTAATAG        56.3  56% | 6  5  | 5  4   5  4  10  9 | ok
>   13 CCCGGGGATCCTAATAGA        57.3  56% | 6  4  | 5  5   5  5  10  8 |
>   14 CCGGGGATCCTAATAGAATC      57.4  50% | 9  9  | 6  6   6  6   9  9 |
>   15 CGGGGATCCTAATAGAATCT      54.9  45% | 9  8  | 5  4   7  7  10 10 |
>   16 GGGGATCCTAATAGAATCTCA     54.9  43% | 9  8  | 5  5   7  6  10 10 |
>   17 GGGATCCTAATAGAATCTCAGA    54.2  41% | 9  5  | 7  7   7  5  10 10 |
>   18 GGATCCTAATAGAATCTCAGAATC  54.5  38% | 9  7  | 8  8   7  6  11 10 | ok
>   19 GATCCTAATAGAATCTCAGAATCT  52.1  33%
>   20 ATCCTAATAGAATCTCAGAATCTG  53.0  33%
>   21 TCCTAATAGAATCTCAGAATCTGA  54.3  33% | 9  9  | 8  7   7  6  10 10 |
>   22 CCTAATAGAATCTCAGAATCTGAG  53.5  38% |11 11  | 8  6   7  6  11 11 |
>   23 CTAATAGAATCTCAGAATCTGAGA  51.7  33%
>   24 TAATAGAATCTCAGAATCTGAGAA  52.2  29%
>   25 AATAGAATCTCAGAATCTGAGAAT  52.5  29%
>   26 ATAGAATCTCAGAATCTGAGAATA  51.2  29%
>   27 TAGAATCTCAGAATCTGAGAATAA  52.2  29%
>   28 AGAATCTCAGAATCTGAGAATAAC  52.9  33%
>   29 GAATCTCAGAATCTGAGAATAACA  54.5  33% |13  8  | 8  5   7  7  10 10 |
>   30 AATCTCAGAATCTGAGAATAACAG  53.8  33% |13  4  | 7  6   7  7  10  9 |
>   31 ATCTCAGAATCTGAGAATAACAGG  55.9  38% |13  3  | 7  7   7  6  10  9 |
>   32 TCTCAGAATCTGAGAATAACAGG   55.4  39% |13  3  | 7  7   7  6  10  9 |
>   33 CTCAGAATCTGAGAATAACAGG    53.4  41% |11  2  | 7  7   7  6  10  9 | ok
>   34 TCAGAATCTGAGAATAACAGGN    53.2  38% | 9  2  | 7  7   6  6  10  9 |
>   35 CAGAATCTGAGAATAACAGGNTC   53.9  41% | 7  5  | 7  5   6  5  10  9 | ok
>   36 AGAATCTGAGAATAACAGGNTCC   54.8  41% | 5  4  | 7  6   6  4  10  8 | ok
>   37 GAATCTGAGAATAACAGGNTCC    53.6  43% | 5  4  | 7  6   6  4  10  8 | ok
>   38 AATCTGAGAATAACAGGNTCCA    54.5  38% | 5  3  | 7  5   6  5  10  9 |
>   39 ATCTGAGAATAACAGGNTCCAT    53.5  38% | 5  3  | 7  4   6  4  10  8 |
>   40 TCTGAGAATAACAGGNTCCATG    55.9  43% | 5  4  | 7  5   5  5  10  8 | ok
>   41 CTGAGAATAACAGGNTCCATG     53.8  45% | 4  4  | 7  5   5  5  10  8 | ok
>   42 TGAGAATAACAGGNTCCATGA     54.6  40% | 4  3  | 6  6   5  5  10  9 |
>   43 GAGAATAACAGGNTCCATGAA     53.3  40% | 4  4  | 6  5   5  4  10 10 |
>   44 AGAATAACAGGNTCCATGAAAT    53.6  33% | 4  4  | 6  5   5  5  10 10 |
>   45 GAATAACAGGNTCCATGAAATG    55.2  38% | 4  3  | 6  5   5  5  11 11 | ok
>   46 AATAACAGGNTCCATGAAATG     53.2  35% | 4  3  | 6  5   5  5  11 11 | ok
>   47 ATAACAGGNTCCATGAAATGA     53.6  35% | 4  2  | 6  4   6  6  11 10 |
>   48 TAACAGGNTCCATGAAATGAN     53.6  37%
>   49 AACAGGNTCCATGAAATGAN      53.3  39%
>   50 ACAGGNTCCATGAAATGANTG     55.4  42%
>   51 CAGGNTCCATGAAATGANTG      54.3  44%
>   52 AGGNTCCATGAAATGANTGTC     54.4  42%
>   53 GGNTCCATGAAATGANTGTC      53.1  44%
>   54 GNTCCATGAAATGANTGTCAA     53.9  37%
>   55 NTCCATGAAATGANTGTCAAA     54.5  32%
>   56 TCCATGAAATGANTGTCAAA      53.8  32% | 5  3  | 5  3   7  7   9  8 |
>   57 CCATGAAATGANTGTCAAAGA     55.1  35% | 5  2  | 5  5   7  5  10 10 |
>   58 CATGAAATGANTGTCAAAGAAA    54.4  29% | 5  2  | 6  6   7  5  11 11 |
>   59 ATGAAATGANTGTCAAAGAAAA    53.1  24% | 5  2  | 6  5   7  4  11 11 |
>   60 TGAAATGANTGTCAAAGAAAAA    54.2  24% | 5  1  | 6  6   7  5  11 10 |
>   61 GAAATGANTGTCAAAGAAAAAGA   54.5  27% | 5  2  | 6  4   7  5  11 10 |
>   62 AAATGANTGTCAAAGAAAAAGAT   53.2  23% | 5  2  | 6  3   7  6  11  9 |
>   63 AATGANTGTCAAAGAAAAAGATT   53.2  23% | 5  3  | 6  4   7  7  11  9 |
>   64 ATGANTGTCAAAGAAAAAGATTC   53.5  27% | 5  3  | 6  4   7  6  11 10 | ok
>   65 TGANTGTCAAAGAAAAAGATTCA   55.7  27% | 5  4  | 6  4   7  7  11 11 |
>   66 GANTGTCAAAGAAAAAGATTCAG   54.1  32% | 3  2  | 6  5   7  6  12 12 | ok
>   67 ANTGTCAAAGAAAAAGATTCAGA   54.1  27% | 3  3  | 6  5   7  6  11 10 |
>   68 NTGTCAAAGAAAAAGATTCAGA    53.5  29% | 3  3  | 6  5   7  6  11 10 |
>   69 TGTCAAAGAAAAAGATTCAGAG    54.0  32% | 3  2  | 6  5   7  6  11  9 | ok
>   70 GTCAAAGAAAAAGATTCAGAGA    53.1  32% | 3  2  | 6  6   7  6  11 10 |
>   71 TCAAAGAAAAAGATTCAGAGAA    53.7  27% | 3  3  | 7  7   7  5  11 11 |
>   72 CAAAGAAAAAGATTCAGAGAAGA   54.9  30% | 3  2  | 7  6   7  6  12 12 |
>   73 AAAGAAAAAGATTCAGAGAAGAG   53.3  30% | 3  1  | 7  5   7  7  12 11 | ok
>   74 AAGAAAAAGATTCAGAGAAGAGA   53.6  30% | 3  2  | 7  5   7  6  12 10 |
>   75 AGAAAAAGATTCAGAGAAGAGAA   53.6  30% | 3  3  | 7  6   7  6  11 11 |
>   76 GAAAAAGATTCAGAGAAGAGAAA   54.0  30% | 3  2  | 7  7   7  5  11 11 |
>   77 AAAAAGATTCAGAGAAGAGAAAG   53.3  30% | 3  1  | 7  6   7  6  12 12 | ok
>   78 AAAAGATTCAGAGAAGAGAAAGC   55.5  35% | 3  2  | 7  7   7  5  11 10 | ok
>   79 AAAGATTCAGAGAAGAGAAAGC    54.0  36% | 3  2  | 7  7   7  5  11 10 | ok
>   80 AAGATTCAGAGAAGAGAAAGCG    57.1  41% | 3  2  | 7  6   7  6  11  9 | ok
>   81 AGATTCAGAGAAGAGAAAGCG     55.7  43% | 3  2  | 7  6   7  6  11  9 | ok
>   82 GATTCAGAGAAGAGAAAGCG      54.4  45% | 3  2  | 7  6   7  6  10  9 | ok
>   83 ATTCAGAGAAGAGAAAGCGG      56.4  45% | 3  1  | 7  5   7  6  10 10 | ok
>   84 TTCAGAGAAGAGAAAGCGG       55.8  47% | 3  1  | 7  5   7  6  10 10 | ok
>   85 TCAGAGAAGAGAAAGCGG        54.1  50% | 3  1  | 7  5   7  5  10 10 | ok
>   86 CAGAGAAGAGAAAGCGGGT       57.2  53% | 2  1  | 7  7   7  4  11 10 |
>   87 AGAGAAGAGAAAGCGGGTC       56.2  53% | 2  2  | 8  8   7  5  11  9 | ok
>   88 GAGAAGAGAAAGCGGGTC        54.9  56% | 2  2  | 8  8   7  5  11  9 | ok
>   89 AGAAGAGAAAGCGGGTCAG       57.2  53% | 2  1  | 8  5   7  6  11 10 | ok
>   90 GAAGAGAAAGCGGGTCAG        56.0  56% | 2  1  | 8  5   6  6  10 10 | ok
>   91 AAGAGAAAGCGGGTCAGC        58.1  56%
>   92 AGAGAAAGCGGGTCAGCT        57.7  56% | 4  4  | 8  4   6  6  11 10 |
>   93 GAGAAAGCGGGTCAGCTG        59.7  61%
>   94 AGAAAGCGGGTCAGCTGA        59.7  56%
>   95 GAAAGCGGGTCAGCTGAG        59.7  61%
>   96 AAAGCGGGTCAGCTGAGT        58.5  56%
>   97 AAGCGGGTCAGCTGAGTA        57.0  56% | 8  5  | 6  5   6  5   9  9 |
>   98 AGCGGGTCAGCTGAGTAATA      57.6  50% | 8  3  | 6  4   6  6  10 10 |
>   99 GCGGGTCAGCTGAGTAATAA      58.0  50% | 8  2  | 6  5   6  6  10  9 |
>  100 CGGGTCAGCTGAGTAATAAA      55.6  45% | 8  1  | 6  6   7  7  10  9 |
>  101 GGGTCAGCTGAGTAATAAACA     54.6  43% | 8  3  | 6  5   7  7  10  8 |
>  102 GGTCAGCTGAGTAATAAACACC    55.6  45% | 8  3  | 6  4   7  6  10  8 | ok
>  103 GTCAGCTGAGTAATAAACACCA    54.7  41% | 8  2  | 6  5   7  7  10  9 |
>  104 TCAGCTGAGTAATAAACACCA     53.6  38% | 8  2  | 6  5   7  7  10  9 |
>  105 CAGCTGAGTAATAAACACCATG    55.1  41% | 6  4  | 6  5   7  7  10  9 | ok
>  106 AGCTGAGTAATAAACACCATGC    56.2  41% | 4  3  | 6  6   7  6  10  9 | ok
>  107 GCTGAGTAATAAACACCATGC     55.0  43% | 4  3  | 6  6   7  6   9  9 | ok
>  108 CTGAGTAATAAACACCATGCA     53.9  38% | 4  4  | 6  5   7  7   9  9 |
>  109 TGAGTAATAAACACCATGCAA     54.3  33% | 4  3  | 6  4   7  7   9  9 |
>  110 GAGTAATAAACACCATGCAAA     53.0  33% | 4  2  | 6  3   7  5  10 10 |
>  111 AGTAATAAACACCATGCAAAAA    54.3  27% | 4  1  | 6  4   7  5  10 10 |
>  112 GTAATAAACACCATGCAAAAA     53.1  29% | 4  1  | 6  4   7  5  10 10 |
>  113 TAATAAACACCATGCAAAAATG    55.5  27% | 4  3  | 6  4   7  5  12 12 | ok
>  114 AATAAACACCATGCAAAAATG     55.3  29% | 4  3  | 6  4   7  5  12 12 | ok
>  115 ATAAACACCATGCAAAAATG      53.7  30% | 4  3  | 6  4   7  5  11 11 | ok
>  116 TAAACACCATGCAAAAATGA      55.1  30% | 4  2  | 6  3   7  6  11 10 |
>  117 AAACACCATGCAAAAATGAA      56.6  30% | 4  2  | 6  3   7  5  11 10 |
>  118 AACACCATGCAAAAATGAAA      56.6  30% | 4  1  | 6  4   7  6  11 11 |
>  119 ACACCATGCAAAAATGAAAC      56.0  35% | 4  1  | 6  5   7  5  10 10 | ok
>  120 CACCATGCAAAAATGAAAC       54.9  37% | 4  1  | 6  5   7  5  10  9 | ok
>  121 ACCATGCAAAAATGAAACAA      56.6  30% | 4  1  | 5  3   6  6  10 10 |
>  122 CCATGCAAAAATGAAACAAA      57.1  30% | 4  1  | 5  3   7  7  10 10 |
>  123 CATGCAAAAATGAAACAAAC      54.3  30% | 4  1  | 5  4   7  6  11 11 | ok
>  124 ATGCAAAAATGAAACAAACA      54.3  25% | 4  2  | 5  3   7  6  11 11 |
>  125 TGCAAAAATGAAACAAACAA      55.4  25% | 4  1  | 5  4   7  7  12 12 |
>  126 GCAAAAATGAAACAAACAAA      54.0  25% | 2  1  | 5  5   8  8  12 11 |
>  127 CAAAAATGAAACAAACAAACC     54.9  29% | 2  1  | 7  7   8  8  12 10 | ok
>  128 AAAAATGAAACAAACAAACCC     55.8  29% | 2  1  | 8  8   8  7  11 11 | ok
>  129 AAAATGAAACAAACAAACCC      54.2  30% | 2  1  | 8  8   8  7  11 11 | ok
>  130 AAATGAAACAAACAAACCCC      56.4  35% | 2  1  | 8  7   8  6  11 10 | ok
>  131 AATGAAACAAACAAACCCCT      56.1  35% | 2  1  | 8  7   8  7  10 10 |
>  132 ATGAAACAAACAAACCCCTT      56.1  35% | 2  2  | 8  7   7  6  11 11 |
>  133 TGAAACAAACAAACCCCTT       55.5  37% | 2  2  | 8  7   7  6  11 11 |
>  134 GAAACAAACAAACCCCTTG       55.5  42% | 3  3  | 8  6   7  5  11 10 | ok
>  135 AAACAAACAAACCCCTTGC       57.6  42% | 3  2  | 8  6   7  5  11 11 | ok
>  136 AACAAACAAACCCCTTGC        56.0  44% | 3  2  | 8  6   6  5  11 11 | ok
>  137 ACAAACAAACCCCTTGCC        58.4  50%
>  138 CAAACAAACCCCTTGCCC        61.3  56%
>  139 AAACAAACCCCTTGCCCC        62.0  56%
>  140 AACAAACCCCTTGCCCCC        64.2  61%
>  141 ACAAACCCCTTGCCCCCT        63.9  61%
>  142 CAAACCCCTTGCCCCCTC        65.0  67%

