REBASE version 909 gcg.909 =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= REBASE, The Restriction Enzyme Database http://rebase.neb.com Copyright (c) Dr. Richard J. Roberts, 1999. All rights reserved. =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Rich Roberts Aug 31 99 REBASE codes for commercial sources of enzymes A Amersham Pharmacia Biotech (11/98) B Life Technologies Inc. (1/98) C Minotech, Molecular Biology Products (3/99) D Angewandte Gentechnologie Systeme (10/97) E Stratagene (1/98) F Fermentas AB (8/99) G Appligene Oncor (10/97) H American Allied Biochemical, Inc. (10/98) I SibEnzyme Ltd. (8/99) J Nippon Gene Co., Ltd. (10/97) K Takara Shuzo Co. Ltd. (11/98) L Kramel Biotech (7/98) M Roche Molecular Biochemicals (3/99) N New England BioLabs (7/99) O Toyobo Biochemicals (11/98) P Megabase Research Products (5/99) Q CHIMERx (10/97) R Promega Corporation (6/99) S Sigma Chemical Corporation (11/98) T Advanced Biotechnologies Ltd. (3/98) .. ; Top Bottom ;Name Offset Recognition Pattern Offset Err dam dcm units $ ! Isoschiz >Supplier Index ;AarI 3 CACCTGC 0 0 100 100 0 0 ? ! > ;AatI 3 AGG'CCT 0 0 100 100 0 0 ! StuI,Eco147I,Pme55I,SseBI >O AatII 5 G_ACGT'C -4 0 100 100 2500 176 ! >ADEFIKLMNOR ;AauI 1 T'GTAC_A 4 0 100 100 0 0 ! Bsp1407I,BsrGI,SspBI >I AccI 2 GT'mk_AC 2 0 100 100 2500 220 ! FblI,XmiI >ABDEGJKLMNOQRS AccII 2 CG'CG 0 0 100 100 444 99 ! Bsh1236I,BstFNI,BstUI,MvnI,ThaI >KQR ;AccIII 1 T'CCGG_A 4 0 100 100 0 0 ! BseAI,BsiMI,Bsp13I,BspEI,Kpn2I,MroI >EJKQR ;Acc16I 3 TGC'GCA 0 0 100 100 0 0 ! AviII,FspI,NsbI >DI ;Acc36I 10 ACCTGCnnnn'nnnn_ 4 0 100 100 0 0 ! BspMI >I Acc65I 1 G'GTAC_C 4 0 100 3 5000 200 ! KpnI,Asp718I >DFINR ;Acc113I 3 AGT'ACT 0 0 100 100 0 0 ! ScaI >I ;AccB1I 1 G'GyrC_C 4 0 100 100 0 0 ! BanI,BshNI,Eco64I >I ;AccB7I 7 CCAn_nnn'nTGG -3 0 100 100 0 0 ! PflMI,Van91I >IR ;AccBSI 3 CCG'CTC 0 0 100 100 0 0 ! BsrBI,MbiI >DI ;AceII 5 G_CTAG'C -4 0 100 100 0 0 ! NheI,AsuNHI > ;AceIII 13 CAGCTCnnnnnnn'nnnn_ 4 0 100 100 0 0 ! > AciI 1 C'CG_C 2 0 100 100 1000 200 ! >N AclI 2 AA'CG_TT 2 0 100 100 1500 200 ! Psp1406I >IN ;AclNI 1 A'CTAG_T 4 0 100 100 0 0 ! SpeI,BcuI >I ;AclWI 9 GGATCnnnn'n_ 1 0 100 100 0 0 ! AlwI,BspPI >I ;AcsI 1 r'AATT_y 4 0 100 100 0 0 ! ApoI,XapI >DIM ;AcyI 2 Gr'CG_yC 2 0 100 100 0 0 ! BsaHI,BstACI,Hin1I,Hsp92I,Msp17I >JM ;AdeI 6 CAC_nnn'GTG -3 0 100 100 0 0 ! DraIII >F ;AfaI 2 GT'AC 0 0 100 100 0 0 ! RsaI,Csp6I >K AfeI 3 AGC'GCT 0 0 100 100 1000 400 ! Eco47III,Aor51HI >IN AflII 1 C'TTAA_G 4 0 100 100 10000 176 ! BfrI,BspTI,Bst98I,MspCI,Vha464I >ABJKNO AflIII 1 A'CryG_T 4 0 100 100 1250 200 ! >ABMNS AgeI 1 A'CCGG_T 4 0 100 100 500 200 ! AsiAI,BshTI,PinAI >JNR ;AhaIII 3 TTT'AAA 0 0 100 100 0 0 ! DraI > AhdI 6 GACnn_n'nnGTC -1 0 100 100 5000 220 ! Eam1105I,AspEI,EclHKI,NruGI >N ; Top Bottom ;Name Offset Recognition Pattern Offset Err dam dcm units $ ! Isoschiz >Supplier Index ;AloI 6 GAACnnnnnnTCC 0 0 100 100 0 0 ? ! > AluI 2 AG'CT 0 0 100 100 2000 200 ! >ABCDEFGHIJKLMNOQRST AlwI 9 GGATCnnnn'n_ 1 0 -8 100 500 200 ! AclWI,BspPI >N ;Alw21I 5 G_wGCw'C -4 0 100 100 0 0 ! AspHI,Bbv12I,BsiHKAI >DF ;Alw26I 6 GTCTCn'nnnn_ 4 0 100 100 0 0 ! BsmAI,BsoMAI >DFR ;Alw44I 1 G'TGCA_C 4 0 100 100 0 0 ! ApaLI,VneI >FJMORS AlwNI 6 CAG_nnn'CTG -3 0 100 -1 2500 200 ! CaiI >ABN ;Ama87I 1 C'yCGr_G 4 0 100 100 0 0 ! AvaI,BcoI,BsoBI,Eco88I >DI ;AocI 2 CC'TnA_GG 3 0 100 100 0 0 ! AxyI,Bse21I,Bsu36I,CvnI,Eco81I >M ;Aor51HI 3 AGC'GCT 0 0 100 100 0 0 ! Eco47III,AfeI >AK ApaI 5 G_GGCC'C -4 0 100 -1 10000 220 ! Bsp120I,PspOMI >ABEFGIJKLMNOQRS ;ApaBI 8 GCA_nnnnn'TGC -5 0 100 100 0 0 ! BstAPI > ApaLI 1 G'TGCA_C 4 0 100 100 5000 200 ! Alw44I,VneI >ADEKN ApoI 1 r'AATT_y 4 0 100 100 2500 220 ! AcsI,XapI >N AscI 2 GG'CGCG_CC 4 0 100 100 2500 200 ! >N AseI 2 AT'TA_AT 2 0 100 100 10000 200 ! VspI,AsnI,PshBI >AJNO ;AsiAI 1 A'CCGG_T 4 0 100 100 0 0 ! AgeI,BshTI,PinAI >I ;AsnI 2 AT'TA_AT 2 0 100 100 0 0 ! VspI,AseI,PshBI >MS ;AspI 4 GACn'n_nGTC 1 0 100 100 0 0 ! Tth111I,PflFI,PsyI >M ;Asp700I 5 GAAnn'nnTTC 0 0 100 100 0 0 ! XmnI,MroXI >M ;Asp718I 1 G'GTAC_C 4 0 100 100 0 0 ! KpnI,Acc65I >M ;AspEI 6 GACnn_n'nnGTC -1 0 100 100 0 0 ! Eam1105I,AhdI,EclHKI,NruGI >M ;AspHI 5 G_wGCw'C -4 0 100 100 0 0 ! Alw21I,Bbv12I,BsiHKAI >M ;AspLEI 3 G_CG'C -2 0 100 100 0 0 ! HhaI,CfoI,Hin6I,HinP1I,HspAI >I ;AspS9I 1 G'GnC_C 3 0 100 100 0 0 ! BsiZI,Cfr13I,Sau96I >I ;AsuI 1 G'GnC_C 3 0 100 100 0 0 ! AspS9I,BsiZI,Cfr13I,Sau96I > ;AsuII 2 TT'CG_AA 2 0 100 100 0 0 ! Bpu14I,BsiCI,Bsp119I,BstBI,CbiI,Csp45I,LspI,NspV,SfuI > ;AsuC2I 2 CC's_GG 1 0 100 100 0 0 ! BcnI,NciI >I ;AsuHPI 13 GGTGAnnnnnnn_n' -1 0 100 100 0 0 ! HphI >I ;AsuNHI 1 G'CTAG_C 4 0 100 100 0 0 ! NheI >I ; Top Bottom ;Name Offset Recognition Pattern Offset Err dam dcm units $ ! Isoschiz >Supplier Index AvaI 1 C'yCGr_G 4 0 100 100 10000 176 ! Ama87I,BcoI,BsoBI,Eco88I >ABEGJKLMNOQRS AvaII 1 G'GwC_C 3 0 100 2 10000 176 ! Bme18I,Eco47I,HgiEI,SinI >ABEGJKMNQRS ;AvaIII 3 ATGCAT 0 0 100 100 0 0 ? ! EcoT22I,Mph1103I,NsiI,Ppu10I,Zsp2I > ;AviII 3 TGC'GCA 0 0 100 100 0 0 ! Acc16I,FspI,NsbI >KM AvrII 1 C'CTAG_G 4 0 100 100 500 220 ! BlnI,BspA2I >N ;AxyI 2 CC'TnA_GG 3 0 100 100 0 0 ! AocI,Bse21I,Bsu36I,CvnI,Eco81I >J BaeI 23 ACnnnnGTAyCnnnnnnn_nnnnn' -5 0 100 100 1250 200 ! >N BaeI 26 GrTACnnnnGTnnnnnnnnnn_nnnnn' -5 0 100 100 1250 200 ! >N ;BalI 3 TGG'CCA 0 0 100 0 0 0 ! MlsI,MluNI,MscI >AJKR BamHI 1 G'GATC_C 4 0 100 100 50000 176 ! >ABCDEFGHIJKLMNOQRST BanI 1 G'GyrC_C 4 0 100 100 25000 200 ! AccB1I,BshNI,Eco64I >AEMNOR BanII 5 G_rGCy'C -4 0 100 100 5000 200 ! Eco24I,EcoT38I,FriOI >ABKLMNOQRS ;BanIII 2 AT'CG_AT 2 0 100 100 0 0 ! ClaI,Bsa29I,BscI,BseCI,BsiXI,Bsp106I,BspDI,BspXI,Bsu15I >O BbeI 5 G_GCGC'C -4 0 100 100 0 0 ! NarI,EgeI,EheI,KasI,Mly113I,SfoI >K ;Bbr7I 13 GAAGACnnnnnnn'nnnn_ 4 0 100 100 0 0 ! BbsI,BpiI,BpuAI > ;BbrPI 3 CAC'GTG 0 0 100 100 0 0 ! PmaCI,Eco72I,PmlI >MO BbsI 8 GAAGACnn'nnnn_ 4 0 100 100 1000 200 ! BpiI,BpuAI >N ;BbuI 5 G_CATG'C -4 0 100 100 0 0 ! SphI,PaeI >R BbvI 13 GCAGCnnnnnnnn'nnnn_ 4 0 100 100 750 200 ! BseXI,Bst71I >N ;BbvII 8 GAAGACnn'nnnn_ 4 0 100 100 0 0 ! BbsI,BpiI,BpuAI > ;Bbv12I 5 G_wGCw'C -4 0 100 100 0 0 ! Alw21I,AspHI,BsiHKAI >I BbvCI 2 CC'TCA_GC 3 0 100 100 500 200 ! >N ;BccI 2 CCATC 0 0 100 100 0 0 ? ! > ;Bce83I 22 CTTGAGnnnnnnnnnnnnnn_nn' -2 0 100 100 0 0 ! > ;BcefI 17 ACGGCnnnnnnnnnnnn'n_ 1 0 100 100 0 0 ! > BcgI 24 CGAnnnnnnTGCnnnnnnnnnn_nn' -2 0 100 100 1250 200 ! >N BcgI 24 GCAnnnnnnTCGnnnnnnnnnn_nn' -2 0 100 100 1250 200 ! >N BciVI 12 GTATCCnnnnn_n' -1 0 100 100 1000 200 ! >N BclI 1 T'GATC_A 4 0 0 100 10000 176 ! BsiQI,FbaI,Ksp22I >BCDEFGJLMNORS ;BcnI 2 CC's_GG 1 0 100 100 0 0 ! AsuC2I,NciI >ADFK ; Top Bottom ;Name Offset Recognition Pattern Offset Err dam dcm units $ ! Isoschiz >Supplier Index ;BcoI 1 C'yCGr_G 4 0 100 100 0 0 ! AvaI,Ama87I,BsoBI,Eco88I >T ;BcuI 1 A'CTAG_T 4 0 100 100 0 0 ! SpeI,AclNI >F ;BetI 1 w'CCGG_w 4 0 100 100 0 0 ! BsaWI > BfaI 1 C'TA_G 2 0 100 100 2500 220 ! MaeI >N ;BfiI 11 ACTGGGnnnn_n' -1 0 100 100 0 0 ! BmrI > ;BfmI 1 C'TryA_G 4 0 100 100 0 0 ! BstSFI,SfcI >F ;BfrI 1 C'TTAA_G 4 0 100 100 0 0 ! AflII,BspTI,Bst98I,MspCI,Vha464I >MO ;BfrBI 3 ATG'CAT 0 0 100 100 0 0 ! EcoT22I,Mph1103I,NsiI,Ppu10I,Zsp2I > BglI 7 GCCn_nnn'nGGC -3 0 100 100 10000 176 ! >ABCDEFGHIJKLMNOQRST BglII 1 A'GATC_T 4 0 100 100 10000 176 ! >ABCDEFGHIJKLMNOQRST ;BinI 9 GGATCnnnn'n_ 1 0 100 100 0 0 ! AclWI,AlwI,BspPI > ;BlnI 1 C'CTAG_G 4 0 100 100 0 0 ! AvrII,BspA2I >AKMS BlpI 2 GC'TnA_GC 3 0 100 100 2500 220 ! Bpu1102I,Bsp1720I,CelII >N ;Bme18I 1 G'GwC_C 3 0 100 100 0 0 ! AvaII,Eco47I,HgiEI,SinI >I ;Bme1390I 2 CC'n_GG 1 0 100 100 0 0 ! ScrFI,BssKI,MspR9I >F ;BmgI 3 GkGCCC 0 0 100 100 0 0 ? ! > BmrI 3 ACTGGG 0 0 100 100 125 200 ? ! >N ;BmyI 5 G_dGCh'C -4 0 100 100 0 0 ! SduI,Bsp1286I >M ;BoxI 5 GACnn'nnGTC 0 0 100 100 0 0 ! PshAI >F ;BpiI 8 GAAGACnn'nnnn_ 4 0 100 100 0 0 ! BbsI,BpuAI >DF BplI 24 GAGnnnnnCTCnnnnnnnn_nnnnn' -5 0 100 100 0 0 ! >F BpmI 22 CTGGAGnnnnnnnnnnnnnn_nn' -2 0 100 -23 0 0 ! GsuI >N Bpu10I 2 CC'TnA_GC 3 0 100 100 0 0 ! >F ;Bpu14I 2 TT'CG_AA 2 0 100 100 0 0 ! BsiCI,Bsp119I,BstBI,CbiI,Csp45I,LspI,NspV,SfuI >I ;Bpu1102I 2 GC'TnA_GC 3 0 100 100 0 0 ! BlpI,Bsp1720I,CelII >BDEFK ;BpuAI 8 GAAGACnn'nnnn_ 4 0 100 100 0 0 ! BbsI,BpiI >M BsaI 7 GGTCTCn'nnnn_ 4 0 100 100 0 0 ! Eco31I,Bso31I >N ;Bsa29I 2 AT'CG_AT 2 0 100 100 0 0 ! ClaI,BanIII,BscI,BseCI,BsiXI,Bsp106I,BspDI,BspXI,Bsu15I >I BsaAI 3 yAC'GTr 0 0 100 100 2500 200 ! BstBAI >N BsaBI 5 GATnn'nnATC 0 0 -5 100 10000 200 ! Bse8I,Bsh1365I,BsiBI,BsrBRI,MamI >N ; Top Bottom ;Name Offset Recognition Pattern Offset Err dam dcm units $ ! Isoschiz >Supplier Index BsaHI 2 Gr'CG_yC 2 0 100 100 5000 200 ! AcyI,BstACI,Hin1I,Hsp92I,Msp17I >N BsaJI 1 C'CnnG_G 4 0 100 100 1250 200 ! BseDI,BssECI >N ;BsaMI 7 GAATG_Cn' -2 0 100 100 0 0 ! BsmI,BscCI,Mva1269I >R ;BsaOI 4 CG_ry'CG -2 0 100 100 0 0 ! Bsh1285I,BsiEI,BstMCI >R BsaWI 1 w'CCGG_w 4 0 100 100 500 220 ! >N ;BsaXI 5 ACnnnnnCTCC 0 0 100 100 0 0 ? ! > ;BsbI 3 CAACAC 0 0 100 100 0 0 ? ! > ;BscI 2 AT'CG_AT 2 0 100 100 0 0 ! ClaI,BanIII,Bsa29I,BseCI,BsiXI,Bsp106I,BspDI,BspXI,Bsu15I >L ;Bsc4I 7 CCnn_nnn'nnGG -3 0 100 100 0 0 ! BsiYI,BseLI,BslI >I ;BscAI 9 GCATCnnnn'nn_ 2 0 100 100 0 0 ! SfaNI > ;BscBI 3 GGn'nCC 0 0 100 100 0 0 ! NlaIV,BspLI,PspN4I >T ;BscCI 7 GAATG_Cn' -2 0 100 100 0 0 ! BsmI,BsaMI,Mva1269I >T ;BscFI 0 'GATC_ 4 0 100 100 0 0 ! MboI,Bsp143I,DpnII,Kzo9I,NdeII,Sau3AI >T ;BscGI 2 CCCGT 0 0 100 100 0 0 ? ! > ;Bse1I 6 ACTG_Gn' -2 0 100 100 0 0 ! BsrI,BseNI,BsrSI >I ;Bse8I 5 GATnn'nnATC 0 0 100 100 0 0 ! BsaBI,Bsh1365I,BsiBI,BsrBRI,MamI >I ;Bse21I 2 CC'TnA_GG 3 0 100 100 0 0 ! AocI,AxyI,Bsu36I,CvnI,Eco81I >I ;Bse118I 1 r'CCGG_y 4 0 100 100 0 0 ! Cfr10I,BsrFI,BssAI >I ;BseAI 1 T'CCGG_A 4 0 100 100 0 0 ! AccIII,BsiMI,Bsp13I,BspEI,Kpn2I,MroI >CM ;BseCI 2 AT'CG_AT 2 0 100 100 0 0 ! ClaI,BanIII,Bsa29I,BscI,BsiXI,Bsp106I,BspDI,BspXI,Bsu15I >C ;BseDI 1 C'CnnG_G 4 0 100 100 0 0 ! BsaJI,BssECI >DF ;Bse3DI 8 GCAATG_nn' -2 0 100 100 0 0 ! BsrDI,BseMI >I ;BseGI 7 GGATG_nn' -2 0 100 100 0 0 ! FokI,BstF5I >F ;BseLI 7 CCnn_nnn'nnGG -3 0 100 100 0 0 ! BsiYI,Bsc4I,BslI >F ;BseMI 8 GCAATG_nn' -2 0 100 100 0 0 ! BsrDI,Bse3DI >F BseMII 15 CTCAGnnnnnnnn_nn' -2 0 100 100 0 0 ! >F ;BseNI 6 ACTG_Gn' -2 0 100 100 0 0 ! BsrI,Bse1I,BsrSI >DF ;BsePI 1 G'CGCG_C 4 0 100 100 0 0 ! BssHII,PauI >I BseRI 16 GAGGAGnnnnnnnn_nn' -2 0 100 100 200 55 ! >N BseSI 5 G_kGCm'C -4 0 100 100 0 0 ! >F ; Top Bottom ;Name Offset Recognition Pattern Offset Err dam dcm units $ ! Isoschiz >Supplier Index ;BseXI 13 GCAGCnnnnnnnn'nnnn_ 4 0 100 100 0 0 ! BbvI,Bst71I >F ;BseX3I 1 C'GGCC_G 4 0 100 100 0 0 ! XmaIII,BstZI,EagI,EclXI,Eco52I >I BsgI 22 GTGCAGnnnnnnnnnnnnnn_nn' -2 0 100 100 250 200 ! >N ;BshI 2 GG'CC 0 0 100 100 0 0 ! HaeIII,BsuRI,PalI >T ;Bsh1236I 2 CG'CG 0 0 100 100 0 0 ! AccII,BstFNI,BstUI,MvnI,ThaI >DEF ;Bsh1285I 4 CG_ry'CG -2 0 100 100 0 0 ! BsaOI,BsiEI,BstMCI >DF ;Bsh1365I 5 GATnn'nnATC 0 0 100 100 0 0 ! BsaBI,Bse8I,BsiBI,BsrBRI,MamI >DF ;BshNI 1 G'GyrC_C 4 0 100 100 0 0 ! AccB1I,BanI,Eco64I >F ;BshTI 1 A'CCGG_T 4 0 100 100 0 0 ! AgeI,AsiAI,PinAI >F ;BsiI 1 C'ACGA_G 4 0 100 100 0 0 ! BssSI,Bst2BI > ;BsiBI 5 GATnn'nnATC 0 0 100 100 0 0 ! BsaBI,Bse8I,Bsh1365I,BsrBRI,MamI >T ;BsiCI 2 TT'CG_AA 2 0 100 100 0 0 ! Bpu14I,Bsp119I,BstBI,CbiI,Csp45I,LspI,NspV,SfuI >T BsiEI 4 CG_ry'CG -2 0 100 100 5000 200 ! BsaOI,Bsh1285I,BstMCI >N BsiHKAI 5 G_wGCw'C -4 0 100 100 5000 200 ! Alw21I,AspHI,Bbv12I >N ;BsiLI 2 CC'w_GG 1 0 100 100 0 0 ! EcoRII,BstNI,BstOI,Bst2UI,MvaI,PspGI >T ;BsiMI 1 T'CCGG_A 4 0 100 100 0 0 ! AccIII,BseAI,Bsp13I,BspEI,Kpn2I,MroI >AT ;BsiQI 1 T'GATC_A 4 0 100 100 0 0 ! BclI,FbaI,Ksp22I >T ;BsiSI 1 C'CG_G 2 0 100 100 0 0 ! HpaII,HapII,MspI >C BsiWI 1 C'GTAC_G 4 0 100 100 1500 200 ! Pfl23II,PspLI,SunI >MNO ;BsiXI 2 AT'CG_AT 2 0 100 100 0 0 ! ClaI,BanIII,Bsa29I,BscI,BseCI,Bsp106I,BspDI,BspXI,Bsu15I >T ;BsiYI 7 CCnn_nnn'nnGG -3 0 100 100 0 0 ! Bsc4I,BseLI,BslI >MT ;BsiZI 1 G'GnC_C 3 0 100 100 0 0 ! AspS9I,Cfr13I,Sau96I >T BslI 7 CCnn_nnn'nnGG -3 0 100 -7 5000 200 ! BsiYI,Bsc4I,BseLI >N BsmI 7 GAATG_Cn' -2 0 100 100 0 0 ! BsaMI,BscCI,Mva1269I >ABDEJLMNOS BsmAI 6 GTCTCn'nnnn_ 4 0 100 100 5000 200 ! Alw26I,BsoMAI >N BsmBI 7 CGTCTCn'nnnn_ 4 0 100 100 1000 220 ! Esp3I >N BsmFI 15 GGGACnnnnnnnnnn'nnnn_ 4 0 100 100 500 220 ! >N ;Bso31I 7 GGTCTCn'nnnn_ 4 0 100 100 0 0 ! Eco31I,BsaI >I ;BsoBI 1 C'yCGr_G 4 0 100 100 0 0 ! AvaI,Ama87I,BcoI,Eco88I >N ;BsoMAI 6 GTCTCn'nnnn_ 4 0 100 100 0 0 ! BsmAI,Alw26I >I ; Top Bottom ;Name Offset Recognition Pattern Offset Err dam dcm units $ ! Isoschiz >Supplier Index ;Bsp13I 1 T'CCGG_A 4 0 100 100 0 0 ! AccIII,BseAI,BsiMI,BspEI,Kpn2I,MroI >I ;Bsp19I 1 C'CATG_G 4 0 100 100 0 0 ! NcoI >I ;Bsp24I 24 GACnnnnnnTGGnnnnnnn_nnnnn' -5 0 100 100 0 0 ! > ;Bsp24I 25 CCAnnnnnnGTCnnnnnnnn_nnnnn' -5 0 100 100 0 0 ! > ;Bsp68I 3 TCG'CGA 0 0 100 100 0 0 ! NruI >F ;Bsp106I 2 AT'CG_AT 2 0 100 100 0 0 ! ClaI,BanIII,Bsa29I,BscI,BseCI,BsiXI,BspDI,BspXI,Bsu15I >E ;Bsp119I 2 TT'CG_AA 2 0 100 100 0 0 ! Bpu14I,BsiCI,BstBI,CbiI,Csp45I,LspI,NspV,SfuI >DF ;Bsp120I 1 G'GGCC_C 4 0 100 3 0 0 ! ApaI,PspOMI >DF ;Bsp143I 0 'GATC_ 4 0 100 100 0 0 ! MboI,BscFI,DpnII,Kzo9I,NdeII,Sau3AI >DF ;Bsp143II 5 r_GCGC'y -4 0 100 100 0 0 ! HaeII,BstH2I >F Bsp1286I 5 G_dGCh'C -4 0 100 100 2500 200 ! SduI,BmyI >ADJKNR ;Bsp1407I 1 T'GTAC_A 4 0 100 100 0 0 ! AauI,BsrGI,SspBI >DFK ;Bsp1720I 2 GC'TnA_GC 3 0 100 100 0 0 ! BlpI,Bpu1102I,CelII >I ;BspA2I 1 C'CTAG_G 4 0 100 100 0 0 ! AvrII,BlnI >I ;BspCI 4 CG_AT'CG -2 0 100 100 0 0 ! PvuI,Ple19I >E ;BspDI 2 AT'CG_AT 2 0 1 100 5000 200 ! ClaI,BanIII,Bsa29I,BscI,BseCI,BsiXI,Bsp106I,BspXI,Bsu15I >N BspEI 1 T'CCGG_A 4 0 3 100 5000 200 ! AccIII,BseAI,BsiMI,Bsp13I,Kpn2I,MroI >N ;BspGI 3 CTGGAC 0 0 100 100 0 0 ? ! > BspHI 1 T'CATG_A 4 0 3 100 2500 200 ! PagI,RcaI >AN ;BspKT6I 3 G_AT'C -2 0 100 100 0 0 ! MboI,BscFI,Bsp143I,DpnII,Kzo9I,NdeII,Sau3AI > ;BspLI 3 GGn'nCC 0 0 100 100 0 0 ! NlaIV,BscBI,PspN4I >F ;BspLU11I 1 A'CATG_T 4 0 100 100 0 0 ! PciI >M BspMI 10 ACCTGCnnnn'nnnn_ 4 0 100 100 500 200 ! Acc36I >N ;BspMII 1 T'CCGG_A 4 0 100 100 0 0 ! AccIII,BseAI,BsiMI,Bsp13I,BspEI,Kpn2I,MroI > ;BspPI 9 GGATCnnnn'n_ 1 0 100 100 0 0 ! AclWI,AlwI >F ;BspTI 1 C'TTAA_G 4 0 100 100 0 0 ! AflII,BfrI,Bst98I,MspCI,Vha464I >DF ;BspXI 2 AT'CG_AT 2 0 100 100 0 0 ! ClaI,BanIII,Bsa29I,BscI,BseCI,BsiXI,Bsp106I,BspDI,Bsu15I >G BsrI 6 ACTG_Gn' -2 0 100 100 2500 200 ! Bse1I,BseNI,BsrSI >N BsrBI 3 CCG'CTC 0 0 100 100 0 0 ! AccBSI,MbiI >N ;BsrBRI 5 GATnn'nnATC 0 0 100 100 0 0 ! BsaBI,Bse8I,Bsh1365I,BsiBI,MamI >R ; Top Bottom ;Name Offset Recognition Pattern Offset Err dam dcm units $ ! Isoschiz >Supplier Index BsrDI 8 GCAATG_nn' -2 0 100 100 500 200 ! Bse3DI,BseMI >N BsrFI 1 r'CCGG_y 4 0 100 100 750 200 ! Cfr10I,Bse118I,BssAI >N BsrGI 1 T'GTAC_A 4 0 100 100 5000 200 ! Bsp1407I,AauI,SspBI >N ;BsrSI 6 ACTG_Gn' -2 0 100 100 0 0 ! BsrI,Bse1I,BseNI >R ;BssAI 1 r'CCGG_y 4 0 100 100 0 0 ! Cfr10I,Bse118I,BsrFI >C ;BssECI 1 C'CnnG_G 4 0 100 100 0 0 ! BsaJI,BseDI >I BssHII 1 G'CGCG_C 4 0 100 100 1000 200 ! BsePI,PauI >ABDEJKLMNOQRS BssKI 0 'CCnGG_ 5 0 100 0 1250 200 ! ScrFI,Bme1390I,MspR9I >N ;BssNAI 3 GTA'TAC 0 0 100 100 0 0 ! Bst1107I,BstZ17I >I BssSI 1 C'ACGA_G 4 0 100 100 1000 220 ! Bst2BI >N ;BssT1I 1 C'CwwG_G 4 0 100 100 0 0 ! StyI,Eco130I,EcoT14I,ErhI >I ;Bst71I 13 GCAGCnnnnnnnn'nnnn_ 4 0 100 100 0 0 ! BbvI,BseXI >R ;Bst98I 1 C'TTAA_G 4 0 100 100 0 0 ! AflII,BfrI,BspTI,MspCI,Vha464I >R ;Bst1107I 3 GTA'TAC 0 0 100 100 0 0 ! BssNAI,BstZ17I >DFKM ;BstACI 2 Gr'CG_yC 2 0 100 100 0 0 ! AcyI,BsaHI,Hin1I,Hsp92I,Msp17I >I BstAPI 7 GCAn_nnn'nTGC -3 0 100 100 1000 300 ! >IN BstBI 2 TT'CG_AA 2 0 100 100 12500 176 ! Bpu14I,BsiCI,Bsp119I,CbiI,Csp45I,LspI,NspV,SfuI >N ;Bst2BI 1 C'ACGA_G 4 0 100 100 0 0 ! BssSI >DI ;BstBAI 3 yAC'GTr 0 0 100 100 0 0 ! BsaAI >I Bst4CI 3 AC_n'GT -1 0 100 100 1000 300 ! TaaI >IN ;BstDEI 1 C'TnA_G 3 0 100 100 0 0 ! DdeI >DI BstDSI 1 C'CryG_G 4 0 100 100 0 0 ! DsaI,BtgI >DI BstEII 1 G'GTnAC_C 5 0 100 100 10000 176 ! BstPI,Eco91I,EcoO65I,PspEI >BDGHJLMNORS BstF5I 7 GGATG_nn' -2 0 100 100 500 300 ! FokI,BseGI >IN ;BstFNI 2 CG'CG 0 0 100 100 0 0 ! AccII,Bsh1236I,BstUI,MvnI,ThaI >I ;BstH2I 5 r_GCGC'y -4 0 100 100 0 0 ! HaeII,Bsp143II >I ;BstHPI 3 GTT'AAC 0 0 100 100 0 0 ! HpaI,KspAI >I ;BstMCI 4 CG_ry'CG -2 0 100 100 0 0 ! BsaOI,Bsh1285I,BsiEI >I BstNI 2 CC'w_GG 1 0 100 100 10000 176 ! EcoRII,BsiLI,BstOI,Bst2UI,MvaI,PspGI >CEN ;BstNSI 5 r_CATG'y -4 0 100 100 0 0 ! NspI,XceI >DI ; Top Bottom ;Name Offset Recognition Pattern Offset Err dam dcm units $ ! Isoschiz >Supplier Index ;BstOI 2 CC'w_GG 1 0 100 100 0 0 ! EcoRII,BsiLI,BstNI,Bst2UI,MvaI,PspGI >R ;BstPI 1 G'GTnAC_C 5 0 100 100 0 0 ! BstEII,Eco91I,EcoO65I,PspEI >K ;BstSFI 1 C'TryA_G 4 0 100 100 0 0 ! BfmI,SfcI >DI ;BstSNI 3 TAC'GTA 0 0 100 100 0 0 ! SnaBI,Eco105I >DI BstUI 2 CG'CG 0 0 100 100 0 0 ! AccII,Bsh1236I,BstFNI,MvnI,ThaI >N ;Bst2UI 2 CC'w_GG 1 0 100 100 0 0 ! EcoRII,BsiLI,BstNI,BstOI,MvaI,PspGI >I BstXI 8 CCAn_nnnn'nTGG -4 0 100 100 5000 200 ! >ABDEFGHJKLMNOQR ;BstX2I 1 r'GATC_y 4 0 100 100 0 0 ! XhoII,BstYI,MflI >DI BstYI 1 r'GATC_y 4 0 100 100 2500 176 ! XhoII,BstX2I,MflI >BN ;BstZI 1 C'GGCC_G 4 0 100 100 0 0 ! XmaIII,BseX3I,EagI,EclXI,Eco52I >R BstZ17I 3 GTA'TAC 0 0 100 100 5000 200 ! BssNAI,Bst1107I >N ;Bsu6I 7 CTCTTCn'nnn_ 3 0 100 100 0 0 ! Ksp632I,Eam1104I,EarI >I ;Bsu15I 2 AT'CG_AT 2 0 100 100 0 0 ! ClaI,BanIII,Bsa29I,BscI,BseCI,BsiXI,Bsp106I,BspDI,BspXI >DF Bsu36I 2 CC'TnA_GG 3 0 100 100 2500 200 ! AocI,AxyI,Bse21I,CvnI,Eco81I >ENR ;BsuRI 2 GG'CC 0 0 100 100 0 0 ! HaeIII,BshI,PalI >FI BtgI 3 CCryGG 0 0 100 100 5000 200 ? ! DsaI,BstDSI >N BtrI 3 CAC'GTC 0 0 100 100 250 300 ! >IN ;BtsI 8 GCAGTG_nn' -2 0 100 100 0 0 ! > Cac8I 3 GCn'nGC 0 0 100 100 500 220 ! >N ;CaiI 6 CAG_nnn'CTG -3 0 100 100 0 0 ! AlwNI >F ;CauII 2 CC's_GG 1 0 100 100 0 0 ! AsuC2I,BcnI,NciI > ;CbiI 2 TT'CG_AA 2 0 100 100 0 0 ! Bpu14I,BsiCI,Bsp119I,BstBI,Csp45I,LspI,NspV,SfuI >J ;CciNI 2 GC'GGCC_GC 4 0 100 100 0 0 ! NotI >I ;CelII 2 GC'TnA_GC 3 0 100 100 0 0 ! BlpI,Bpu1102I,Bsp1720I >ALM ;CfoI 3 G_CG'C -2 0 100 100 0 0 ! HhaI,AspLEI,Hin6I,HinP1I,HspAI >BLMRS ;CfrI 1 y'GGCC_r 4 0 100 100 0 0 ! EaeI >F ;Cfr9I 1 C'CCGG_G 4 0 100 100 0 0 ! SmaI,PspAI,XmaI,XmaCI >FO ;Cfr10I 1 r'CCGG_y 4 0 100 100 0 0 ! Bse118I,BsrFI,BssAI >ADFKMO ;Cfr13I 1 G'GnC_C 3 0 100 100 0 0 ! AspS9I,BsiZI,Sau96I >DFKO ;Cfr42I 4 CC_GC'GG -2 0 100 100 0 0 ! SacII,KspI,Sfr303I,SstII >DF ; Top Bottom ;Name Offset Recognition Pattern Offset Err dam dcm units $ ! Isoschiz >Supplier Index ;ChaI 4 _GATC' -4 0 100 100 0 0 ! MboI,BscFI,Bsp143I,DpnII,Kzo9I,NdeII,Sau3AI > ;CjeI 26 CCAnnnnnnGTnnnnnnnnn_nnnnnn' -6 0 100 100 0 0 ! > ;CjeI 25 ACnnnnnnTGGnnnnnnnn_nnnnnn' -6 0 100 100 0 0 ! > ;CjePI 26 CCAnnnnnnnTCnnnnnnnn_nnnnnn' -6 0 100 100 0 0 ! > ;CjePI 25 GAnnnnnnnTGGnnnnnnn_nnnnnn' -6 0 100 100 0 0 ! > ClaI 2 AT'CG_AT 2 0 1 100 5000 200 ! BanIII,Bsa29I,BscI,BseCI,BsiXI,Bsp106I,BspDI,BspXI,Bsu15I >ABHKMNRST ;CpoI 2 CG'GwC_CG 3 0 100 100 0 0 ! RsrII,CspI,Rsr2I >ADFK ;CspI 2 CG'GwC_CG 3 0 100 100 0 0 ! RsrII,CpoI,Rsr2I >EOR Csp6I 1 G'TA_C 2 0 100 100 0 0 ! RsaI,AfaI >DF ;Csp45I 2 TT'CG_AA 2 0 100 100 0 0 ! Bpu14I,BsiCI,Bsp119I,BstBI,CbiI,LspI,NspV,SfuI >OR ;CviAII 1 C'AT_G 2 0 100 100 0 0 ! NlaIII,Hsp92II > CviJI 2 rG'Cy 0 0 100 100 0 0 ! CviTI >Q CviRI 2 TG'CA 0 0 100 100 0 0 ! >P ;CviTI 2 rG'Cy 0 0 100 100 0 0 ! CviJI >P ;CvnI 2 CC'TnA_GG 3 0 100 100 0 0 ! AocI,AxyI,Bse21I,Bsu36I,Eco81I >B DdeI 1 C'TnA_G 3 0 100 100 2500 176 ! BstDEI >ABEGLMNORS DpnI 2 GA'TC 0 0 100 100 5000 200 ! >ABELMNRS ;DpnII 0 'GATC_ 4 0 0 100 0 0 ! MboI,BscFI,Bsp143I,Kzo9I,NdeII,Sau3AI >N DraI 3 TTT'AAA 0 0 100 100 10000 200 ! >ABDEFGIJKLMNOQRS ;DraII 2 rG'GnC_Cy 3 0 100 100 0 0 ! EcoO109I >AGM DraIII 6 CAC_nnn'GTG -3 0 100 100 5000 200 ! AdeI >AEGMN DrdI 7 GACnn_nn'nnGTC -2 0 100 100 1500 200 ! DseDI >N ;DrdII 3 GAACCA 0 0 100 100 0 0 ? ! > ;DsaI 1 C'CryG_G 4 0 100 100 0 0 ! BstDSI,BtgI >M ;DseDI 7 GACnn_nn'nnGTC -2 0 100 100 0 0 ! DrdI >I EaeI 1 y'GGCC_r 4 0 100 2 500 200 ! CfrI >ADKLMN EagI 1 C'GGCC_G 4 0 100 100 2500 200 ! XmaIII,BseX3I,BstZI,EclXI,Eco52I >N ;Eam1104I 7 CTCTTCn'nnn_ 3 0 100 100 0 0 ! Ksp632I,Bsu6I,EarI >DEF ;Eam1105I 6 GACnn_n'nnGTC -1 0 100 100 0 0 ! AhdI,AspEI,EclHKI,NruGI >DFK EarI 7 CTCTTCn'nnn_ 3 0 100 100 2500 200 ! Ksp632I,Bsu6I,Eam1104I >N ; Top Bottom ;Name Offset Recognition Pattern Offset Err dam dcm units $ ! Isoschiz >Supplier Index EciI 17 GGCGGAnnnnnnnnn_nn' -2 0 100 100 250 200 ! >N Ecl136II 3 GAG'CTC 0 0 100 100 0 0 ! SacI,EcoICRI,Psp124BI,SstI >DF ;EclHKI 6 GACnn_n'nnGTC -1 0 100 100 0 0 ! Eam1105I,AhdI,AspEI,NruGI >R ;EclXI 1 C'GGCC_G 4 0 100 100 0 0 ! XmaIII,BseX3I,BstZI,EagI,Eco52I >MS ;Eco24I 5 G_rGCy'C -4 0 100 100 0 0 ! BanII,EcoT38I,FriOI >DF ;Eco31I 7 GGTCTCn'nnnn_ 4 0 100 100 0 0 ! BsaI,Bso31I >DF ;Eco32I 3 GAT'ATC 0 0 100 100 0 0 ! EcoRV >F ;Eco47I 1 G'GwC_C 3 0 100 100 0 0 ! AvaII,Bme18I,HgiEI,SinI >FO ;Eco47III 3 AGC'GCT 0 0 100 100 0 0 ! AfeI,Aor51HI >BDEFLMOR ;Eco52I 1 C'GGCC_G 4 0 100 100 0 0 ! XmaIII,BseX3I,BstZI,EagI,EclXI >ADFKOR Eco57I 22 CTGAAGnnnnnnnnnnnnnn_nn' -2 0 100 100 0 0 ! >DF ;Eco64I 1 G'GyrC_C 4 0 100 100 0 0 ! AccB1I,BanI,BshNI >D ;Eco72I 3 CAC'GTG 0 0 100 100 0 0 ! PmaCI,BbrPI,PmlI >DEF ;Eco81I 2 CC'TnA_GG 3 0 100 100 0 0 ! AocI,AxyI,Bse21I,Bsu36I,CvnI >ADFKO ;Eco88I 1 C'yCGr_G 4 0 100 100 0 0 ! AvaI,Ama87I,BcoI,BsoBI >F ;Eco91I 1 G'GTnAC_C 5 0 100 100 0 0 ! BstEII,BstPI,EcoO65I,PspEI >F ;Eco105I 3 TAC'GTA 0 0 100 100 0 0 ! SnaBI,BstSNI >FO ;Eco130I 1 C'CwwG_G 4 0 100 100 0 0 ! StyI,BssT1I,EcoT14I,ErhI >DF ;Eco147I 3 AGG'CCT 0 0 100 100 0 0 ! StuI,AatI,Pme55I,SseBI >DF ;EcoHI 0 'CCsGG_ 5 0 100 100 0 0 ! AsuC2I,BcnI,NciI > ;EcoICRI 3 GAG'CTC 0 0 100 100 0 0 ! SacI,Ecl136II,Psp124BI,SstI >R EcoNI 5 CCTnn'n_nnAGG 1 0 100 100 5000 200 ! XagI >AN ;EcoO65I 1 G'GTnAC_C 5 0 100 100 0 0 ! BstEII,BstPI,Eco91I,PspEI >AK EcoO109I 2 rG'GnC_Cy 3 0 100 2 10000 200 ! DraII >ABDEFJKLN EcoRI 1 G'AATT_C 4 0 100 100 50000 176 ! >ABCDEFGHIJKLMNOQRST ;EcoRII 0 'CCwGG_ 5 0 100 0 0 0 ! BsiLI,BstNI,BstOI,Bst2UI,MvaI,PspGI >BEJMOS EcoRV 3 GAT'ATC 0 0 100 100 20000 176 ! Eco32I >ABCDEGHIJKLMNOQRST ;EcoT14I 1 C'CwwG_G 4 0 100 100 0 0 ! StyI,BssT1I,Eco130I,ErhI >AK ;EcoT22I 5 A_TGCA'T -4 0 100 100 0 0 ! Mph1103I,NsiI,Ppu10I,Zsp2I >AKO ;EcoT38I 5 G_rGCy'C -4 0 100 100 0 0 ! BanII,Eco24I,FriOI >J ; Top Bottom ;Name Offset Recognition Pattern Offset Err dam dcm units $ ! Isoschiz >Supplier Index ;EgeI 3 GGC'GCC 0 0 100 100 0 0 ! NarI,BbeI,EheI,KasI,Mly113I,SfoI >I ;EheI 3 GGC'GCC 0 0 100 100 0 0 ! NarI,BbeI,EgeI,KasI,Mly113I,SfoI >ADFO ;ErhI 1 C'CwwG_G 4 0 100 100 0 0 ! StyI,BssT1I,Eco130I,EcoT14I >I ;EspI 2 GC'TnA_GC 3 0 100 100 0 0 ! BlpI,Bpu1102I,Bsp1720I,CelII > ;Esp3I 7 CGTCTCn'nnnn_ 4 0 100 100 0 0 ! BsmBI >DF FauI 9 CCCGCnnnn'nn_ 2 0 100 100 250 300 ! >IN ;FauNDI 2 CA'TA_TG 2 0 100 100 0 0 ! NdeI >DI ;FbaI 1 T'GATC_A 4 0 100 100 0 0 ! BclI,BsiQI,Ksp22I >AK ;FblI 2 GT'mk_AC 2 0 100 100 0 0 ! AccI,XmiI >I ;FinI 2 GGGAC 0 0 100 100 0 0 ? ! BsmFI > ;FmuI 4 G_GnC'C -3 0 100 100 0 0 ! AspS9I,BsiZI,Cfr13I,Sau96I > ;FnuDII 2 CG'CG 0 0 100 100 0 0 ! AccII,Bsh1236I,BstFNI,BstUI,MvnI,ThaI > Fnu4HI 2 GC'n_GC 1 0 100 100 1000 200 ! Fsp4HI,ItaI >N FokI 14 GGATGnnnnnnnnn'nnnn_ 4 0 100 100 5000 200 ! BseGI,BstF5I >ADGIJKLMNR ;FriOI 5 G_rGCy'C -4 0 100 100 0 0 ! BanII,Eco24I,EcoT38I >I FseI 6 GG_CCGG'CC -4 0 100 100 500 220 ! >AKN FspI 3 TGC'GCA 0 0 100 100 2500 200 ! Acc16I,AviII,NsbI >ABJNO ;Fsp4HI 2 GC'n_GC 1 0 100 100 0 0 ! Fnu4HI,ItaI >I ;GdiII 1 C'GGCC_r 4 0 100 100 0 0 ! > ;GsuI 22 CTGGAGnnnnnnnnnnnnnn_nn' -2 0 100 100 0 0 ! BpmI >DF ;HaeI 3 wGG'CCw 0 0 100 100 0 0 ! > HaeII 5 r_GCGC'y -4 0 100 100 10000 176 ! Bsp143II,BstH2I >ABDEGJKLMNORS HaeIII 2 GG'CC 0 0 100 100 15000 176 ! BshI,BsuRI,PalI >ABCDGHIJKLMNOQRST ;HaeIV 5 GAynnnnnrTC 0 0 100 100 0 0 ? ! > ;HapII 1 C'CG_G 2 0 100 100 0 0 ! HpaII,BsiSI,MspI >AK HgaI 10 GACGCnnnnn'nnnnn_ 5 0 100 100 500 200 ! >AN ;HgiAI 5 G_wGCw'C -4 0 100 100 0 0 ! Alw21I,AspHI,Bbv12I,BsiHKAI > ;HgiCI 1 G'GyrC_C 4 0 100 100 0 0 ! AccB1I,BanI,BshNI,Eco64I > ;HgiEI 1 G'GwC_C 3 0 100 100 0 0 ! AvaII,Bme18I,Eco47I,SinI >D ;HgiEII 6 ACCnnnnnnGGT 0 0 100 100 0 0 ? ! > ; Top Bottom ;Name Offset Recognition Pattern Offset Err dam dcm units $ ! Isoschiz >Supplier Index ;HgiJII 5 G_rGCy'C -4 0 100 100 0 0 ! BanII,Eco24I,EcoT38I,FriOI > HhaI 3 G_CG'C -2 0 100 100 10000 176 ! AspLEI,CfoI,Hin6I,HinP1I,HspAI >ABGJKNOR ;Hin1I 2 Gr'CG_yC 2 0 100 100 0 0 ! AcyI,BsaHI,BstACI,Hsp92I,Msp17I >EFKO ;Hin4I 5 GAbnnnnnvTC 0 0 100 100 0 0 ? ! > ;Hin6I 1 G'CG_C 2 0 100 100 0 0 ! HhaI,AspLEI,CfoI,HinP1I,HspAI >DF HinP1I 1 G'CG_C 2 0 100 100 0 0 ! HhaI,AspLEI,CfoI,Hin6I,HspAI >N HincII 3 GTy'rAC 0 0 100 100 5000 176 ! HindII >ABCDEFGHJKLNOQR ;HindII 3 GTy'rAC 0 0 100 100 0 0 ! HincII >MS HindIII 1 A'AGCT_T 4 0 100 100 50000 176 ! >ABCDEFGHIJKLMNOQRST HinfI 1 G'AnT_C 3 0 100 100 25000 176 ! >ABDEFGHIJKLMNOQRST HpaI 3 GTT'AAC 0 0 100 100 2500 176 ! BstHPI,KspAI >ABDEGHJKLMNOQRST HpaII 1 C'CG_G 2 0 100 100 10000 176 ! BsiSI,HapII,MspI >BDEFGILMNOQRS HphI 13 GGTGAnnnnnnn_n' -1 0 -10 100 2000 176 ! AsuHPI >AFN ;Hpy178III 2 TC'nn_GA 2 0 100 100 0 0 ! > ;Hpy188I 3 TC_n'GA -1 0 100 100 0 0 ! > ;Hsp92I 2 Gr'CG_yC 2 0 100 100 0 0 ! AcyI,BsaHI,BstACI,Hin1I,Msp17I >R ;Hsp92II 4 _CATG' -4 0 100 100 0 0 ! NlaIII >R ;HspAI 1 G'CG_C 2 0 100 100 0 0 ! HhaI,AspLEI,CfoI,Hin6I,HinP1I >I ;ItaI 2 GC'n_GC 1 0 100 100 0 0 ! Fnu4HI,Fsp4HI >M KasI 1 G'GCGC_C 4 0 100 100 0 0 ! NarI,BbeI,EgeI,EheI,Mly113I,SfoI >N KpnI 5 G_GTAC'C -4 0 100 100 1250 200 ! Acc65I,Asp718I >ABCDEFGHIJKLMNOQRST ;Kpn2I 1 T'CCGG_A 4 0 100 100 15000 176 ! AccIII,BseAI,BsiMI,Bsp13I,BspEI,MroI >BDF ;KspI 4 CC_GC'GG -2 0 100 100 0 0 ! SacII,Cfr42I,Sfr303I,SstII >MS ;Ksp22I 1 T'GATC_A 4 0 100 100 0 0 ! BclI,BsiQI,FbaI >I ;Ksp632I 7 CTCTTCn'nnn_ 3 0 100 100 0 0 ! Bsu6I,Eam1104I,EarI >M ;KspAI 3 GTT'AAC 0 0 100 100 0 0 ! HpaI,BstHPI >F ;Kzo9I 0 'GATC_ 4 0 100 100 0 0 ! MboI,BscFI,Bsp143I,DpnII,NdeII,Sau3AI >I ;LpnI 3 rGC'GCy 0 0 100 100 0 0 ! HaeII,Bsp143II,BstH2I > ;LspI 2 TT'CG_AA 2 0 100 100 0 0 ! Bpu14I,BsiCI,Bsp119I,BstBI,CbiI,Csp45I,NspV,SfuI >L ;MaeI 1 C'TA_G 2 0 100 100 0 0 ! BfaI >M ; Top Bottom ;Name Offset Recognition Pattern Offset Err dam dcm units $ ! Isoschiz >Supplier Index MaeII 1 A'CG_T 2 0 100 100 0 0 ! TaiI,TscI >M MaeIII 0 'GTnAC_ 5 0 100 100 0 0 ! >M ;MamI 5 GATnn'nnATC 0 0 100 100 0 0 ! BsaBI,Bse8I,Bsh1365I,BsiBI,BsrBRI >M ;MbiI 3 CCG'CTC 0 0 100 100 0 0 ! BsrBI,AccBSI >F ;MboI 0 'GATC_ 4 0 0 100 2500 220 ! BscFI,Bsp143I,DpnII,Kzo9I,NdeII,Sau3AI >ABCEFGKLNQRT MboII 13 GAAGAnnnnnnn_n' -1 0 -10 100 1250 200 ! >ABDFGJKNOQR ;McrI 4 CG_ry'CG -2 0 100 100 0 0 ! BsaOI,Bsh1285I,BsiEI,BstMCI > MfeI 1 C'AATT_G 4 0 100 100 2500 220 ! MunI >N ;MflI 1 r'GATC_y 4 0 100 100 0 0 ! XhoII,BstX2I,BstYI >AK ;MjaIV 3 GTnnAC 0 0 100 100 0 0 ? ! > ;MlsI 3 TGG'CCA 0 0 100 100 0 0 ! BalI,MluNI,MscI >F MluI 1 A'CGCG_T 4 0 100 100 5000 200 ! >ABDEFGHIJKLMNOQRS ;MluNI 3 TGG'CCA 0 0 100 100 0 0 ! BalI,MlsI,MscI >MS MlyI 10 GAGTCnnnnn' 0 0 100 100 500 200 ! PleI,PpsI,SchI >N ;Mly113I 2 GG'CG_CC 2 0 100 100 0 0 ! NarI,BbeI,EgeI,EheI,KasI,SfoI >I ;MmeI 26 TCCrACnnnnnnnnnnnnnnnnnn_nn' -2 0 100 100 0 0 ! > MnlI 11 CCTCnnnnnn_n' -1 0 100 100 1250 200 ! >AEFNQ ;Mph1103I 5 A_TGCA'T -4 0 100 100 0 0 ! EcoT22I,NsiI,Ppu10I,Zsp2I >F ;MroI 1 T'CCGG_A 4 0 100 100 0 0 ! AccIII,BseAI,BsiMI,Bsp13I,BspEI,Kpn2I >MO ;MroNI 1 G'CCGG_C 4 0 100 100 0 0 ! NaeI,NgoAIV,NgoMIV >I ;MroXI 5 GAAnn'nnTTC 0 0 100 100 0 0 ! XmnI,Asp700I >I MscI 3 TGG'CCA 0 0 100 0 500 200 ! BalI,MlsI,MluNI >BDNO MseI 1 T'TA_A 2 0 100 100 1000 200 ! Tru1I,Tru9I >BN MslI 5 CAynn'nnrTG 0 0 100 100 1000 220 ! >N ;MspI 1 C'CG_G 2 0 100 100 25000 176 ! HpaII,BsiSI,HapII >ABDEFGHIJKLMNOQRS ;Msp17I 2 Gr'CG_yC 2 0 100 100 0 0 ! AcyI,BsaHI,BstACI,Hin1I,Hsp92I >D MspA1I 3 CmG'CkG 0 0 100 100 2500 220 ! NspBII >INR ;MspCI 1 C'TTAA_G 4 0 100 100 0 0 ! AflII,BfrI,BspTI,Bst98I,Vha464I >C ;MspR9I 2 CC'n_GG 1 0 100 100 0 0 ! ScrFI,Bme1390I,BssKI >I ;MssI 4 GTTT'AAAC 0 0 100 100 0 0 ! PmeI >F ; Top Bottom ;Name Offset Recognition Pattern Offset Err dam dcm units $ ! Isoschiz >Supplier Index ;MstI 3 TGC'GCA 0 0 100 100 0 0 ! Acc16I,AviII,FspI,NsbI > ;MunI 1 C'AATT_G 4 0 100 100 0 0 ! MfeI >BDEFKM ;MvaI 2 CC'w_GG 1 0 100 100 0 0 ! EcoRII,BsiLI,BstNI,BstOI,Bst2UI,PspGI >ADFKMOS ;Mva1269I 7 GAATG_Cn' -2 0 100 100 0 0 ! BsmI,BsaMI,BscCI >F ;MvnI 2 CG'CG 0 0 100 100 0 0 ! AccII,Bsh1236I,BstFNI,BstUI,ThaI >M MwoI 7 GCnn_nnn'nnGC -3 0 100 100 1250 220 ! >N NaeI 3 GCC'GGC 0 0 100 100 2500 176 ! MroNI,NgoAIV,NgoMIV >ADEKLMNOR NarI 2 GG'CG_CC 2 0 100 100 1000 176 ! BbeI,EgeI,EheI,KasI,Mly113I,SfoI >BEJMNORS NciI 2 CC's_GG 1 0 100 100 10000 200 ! AsuC2I,BcnI >BEJLMNORST NcoI 1 C'CATG_G 4 0 100 100 5000 176 ! Bsp19I >ABCDEFGHJKLMNOQRST NdeI 2 CA'TA_TG 2 0 100 100 20000 200 ! FauNDI >ABDEFGJKLMNRS ;NdeII 0 'GATC_ 4 0 100 100 0 0 ! MboI,BscFI,Bsp143I,DpnII,Kzo9I,Sau3AI >BDGJMRS ;NgoAIV 1 G'CCGG_C 4 0 100 100 0 0 ! NaeI,MroNI,NgoMIV >B NgoMIV 1 G'CCGG_C 4 0 100 100 5000 200 ! NaeI,MroNI,NgoAIV >NR NheI 1 G'CTAG_C 4 0 100 100 2500 200 ! AsuNHI >ABDEFGJKLMNORS NlaIII 4 _CATG' -4 0 100 100 2500 200 ! Hsp92II >AN NlaIV 3 GGn'nCC 0 0 100 100 1000 200 ! BscBI,BspLI,PspN4I >N ;Nli3877I 5 C_yCGr'G -4 0 100 100 0 0 ! AvaI,Ama87I,BcoI,BsoBI,Eco88I > NotI 2 GC'GGCC_GC 4 0 100 100 2500 220 ! CciNI >ABCDEFGHJKLMNOQRST NruI 3 TCG'CGA 0 0 1 100 5000 176 ! Bsp68I >ABCDEGIJKLMNOQRST ;NruGI 6 GACnn_n'nnGTC -1 0 100 100 0 0 ! Eam1105I,AhdI,AspEI,EclHKI >I ;NsbI 3 TGC'GCA 0 0 100 100 0 0 ! Acc16I,AviII,FspI >F NsiI 5 A_TGCA'T -4 0 100 100 5000 200 ! EcoT22I,Mph1103I,Ppu10I,Zsp2I >BDEHJLMNRS NspI 5 r_CATG'y -4 0 100 100 1250 200 ! BstNSI,XceI >ABMN ;NspV 2 TT'CG_AA 2 0 100 100 0 0 ! Bpu14I,BsiCI,Bsp119I,BstBI,CbiI,Csp45I,LspI,SfuI >ABJKO ;NspBII 3 CmG'CkG 0 0 100 100 0 0 ! MspA1I >A PacI 5 TTA_AT'TAA -2 0 100 100 500 200 ! >NO ;PaeI 5 G_CATG'C -4 0 100 100 0 0 ! SphI,BbuI >F ;PaeR7I 1 C'TCGA_G 4 0 100 100 10000 176 ! XhoI,Sfr274I,TliI >N ;PagI 1 T'CATG_A 4 0 100 100 0 0 ! BspHI,RcaI >F ; Top Bottom ;Name Offset Recognition Pattern Offset Err dam dcm units $ ! Isoschiz >Supplier Index ;PalI 2 GG'CC 0 0 100 100 0 0 ! HaeIII,BshI,BsuRI >E ;PauI 1 G'CGCG_C 4 0 100 100 0 0 ! BsePI,BssHII >F PciI 1 A'CATG_T 4 0 100 100 1000 300 ! BspLU11I >IN ;Pfl23II 1 C'GTAC_G 4 0 100 100 0 0 ! BsiWI,PspLI,SunI >DF ;Pfl1108I 3 TCGTAG 0 0 100 100 0 0 ? ! > ;PflFI 4 GACn'n_nGTC 1 0 100 100 5000 200 ! Tth111I,AspI,PsyI >N PflMI 7 CCAn_nnn'nTGG -3 0 100 -7 5000 200 ! AccB7I,Van91I >AN ;PinAI 1 A'CCGG_T 4 0 100 100 0 0 ! AgeI,AsiAI,BshTI >BM PleI 9 GAGTCnnnn'n_ 1 0 100 100 125 320 ! MlyI,PpsI,SchI >N ;Ple19I 4 CG_AT'CG -2 0 100 100 0 0 ! PvuI,BspCI >I ;PmaCI 3 CAC'GTG 0 0 100 100 0 0 ! BbrPI,Eco72I,PmlI >AK PmeI 4 GTTT'AAAC 0 0 100 100 2500 220 ! MssI >N ;Pme55I 3 AGG'CCT 0 0 100 100 0 0 ! StuI,AatI,Eco147I,SseBI >I PmlI 3 CAC'GTG 0 0 100 100 5000 200 ! PmaCI,BbrPI,Eco72I >N ;PpiI 6 GAGnnnnnGTTC 0 0 100 100 0 0 ? ! > ;PpsI 9 GAGTCnnnn'n_ 1 0 100 100 0 0 ! PleI,MlyI,SchI >I Ppu10I 1 A'TGCA_T 4 0 100 100 0 0 ! EcoT22I,Mph1103I,NsiI,Zsp2I >D PpuMI 2 rG'GwC_Cy 3 0 100 2 500 200 ! Psp5II,PspPPI >ANO PshAI 5 GACnn'nnGTC 0 0 100 100 5000 200 ! BoxI >AKN ;PshBI 2 AT'TA_AT 2 0 100 100 0 0 ! VspI,AseI,AsnI >K PsiI 3 TTA'TAA 0 0 100 100 500 400 ! >IN ;Psp5II 2 rG'GwC_Cy 3 0 100 100 0 0 ! PpuMI,PspPPI >BDF ;Psp1406I 2 AA'CG_TT 2 0 100 100 0 0 ! AclI >DFKM ;PspAI 1 C'CCGG_G 4 0 100 100 0 0 ! SmaI,Cfr9I,XmaI,XmaCI >E ;Psp124BI 5 G_AGCT'C -4 0 100 100 0 0 ! SacI,Ecl136II,EcoICRI,SstI >DI ;PspEI 1 G'GTnAC_C 5 0 100 100 0 0 ! BstEII,BstPI,Eco91I,EcoO65I >I PspGI 0 'CCwGG_ 5 0 100 100 500 200 ! EcoRII,BsiLI,BstNI,BstOI,Bst2UI,MvaI >N ;PspLI 1 C'GTAC_G 4 0 100 100 0 0 ! BsiWI,Pfl23II,SunI >I ;PspN4I 3 GGn'nCC 0 0 100 100 0 0 ! NlaIV,BscBI,BspLI >I PspOMI 1 G'GGCC_C 4 0 100 100 5000 200 ! ApaI,Bsp120I >IN ; Top Bottom ;Name Offset Recognition Pattern Offset Err dam dcm units $ ! Isoschiz >Supplier Index ;PspPPI 2 rG'GwC_Cy 3 0 100 100 0 0 ! PpuMI,Psp5II >I ;PssI 5 rG_GnC'Cy -3 0 100 100 0 0 ! DraII,EcoO109I > PstI 5 C_TGCA'G -4 0 100 100 50000 200 ! >ABCDEFGHIJKLMNOQRST ;PsyI 4 GACn'n_nGTC 1 0 100 100 0 0 ! Tth111I,AspI,PflFI >F PvuI 4 CG_AT'CG -2 0 100 100 750 220 ! BspCI,Ple19I >ABDEFGKLMNOQRS PvuII 3 CAG'CTG 0 0 100 100 25000 176 ! >ABCDEFGHIJKLMNOQRST ;RcaI 1 T'CATG_A 4 0 100 100 0 0 ! BspHI,PagI >BM ;RleAI 18 CCCACAnnnnnnnnn_nnn' -3 0 100 100 0 0 ! > RsaI 2 GT'AC 0 0 100 100 5000 176 ! AfaI,Csp6I >ABCDEFGHIJLMNOQRST RsrII 2 CG'GwC_CG 3 0 100 100 1000 220 ! CpoI,CspI,Rsr2I >BMN ;Rsr2I 2 CG'GwC_CG 3 0 100 100 0 0 ! RsrII,CpoI,CspI >I SacI 5 G_AGCT'C -4 0 100 100 10000 176 ! Ecl136II,EcoICRI,Psp124BI,SstI >ACEFGHJKLMNOQRST SacII 4 CC_GC'GG -2 0 100 100 10000 176 ! Cfr42I,KspI,Sfr303I,SstII >ACEGHJKLNOQRT SalI 1 G'TCGA_C 4 0 100 100 10000 176 ! >ABCDEFGHIJKLMNOQRST SanDI 2 GG'GwC_CC 3 0 100 100 0 0 ! >E SapI 8 GCTCTTCn'nnn_ 3 0 100 100 250 200 ! >N ;SauI 2 CC'TnA_GG 3 0 100 100 0 0 ! AocI,AxyI,Bse21I,Bsu36I,CvnI,Eco81I > Sau96I 1 G'GnC_C 3 0 100 2 5000 176 ! AspS9I,BsiZI,Cfr13I >AJLMNOR Sau3AI 0 'GATC_ 4 0 100 100 1000 176 ! MboI,BscFI,Bsp143I,DpnII,Kzo9I,NdeII >ABEGHJKLMNOQRS SbfI 6 CC_TGCA'GG -4 0 100 100 1000 400 ! Sse8387I,SdaI >DIN ScaI 3 AGT'ACT 0 0 100 100 5000 200 ! Acc113I >ABCDEFGJKLMNOQRS ;SchI 10 GAGTCnnnnn' 0 0 100 100 0 0 ! PleI,MlyI,PpsI >F ;SciI 3 CTC'GAG 0 0 100 100 0 0 ! XhoI,PaeR7I,Sfr274I,TliI > ScrFI 2 CC'n_GG 1 0 100 -2 5000 200 ! Bme1390I,BssKI,MspR9I >DJMNOS ;SdaI 6 CC_TGCA'GG -4 0 100 100 0 0 ! Sse8387I,SbfI >F ;SduI 5 G_dGCh'C -4 0 100 100 0 0 ! BmyI,Bsp1286I >F ;SecI 1 C'CnnG_G 4 0 100 100 0 0 ! BsaJI,BseDI,BssECI > ;SelI 0 'CGCG_ 4 0 100 100 0 0 ! AccII,Bsh1236I,BstFNI,BstUI,MvnI,ThaI > SexAI 1 A'CCwGG_T 5 0 100 0 1000 200 ! >MN SfaNI 10 GCATCnnnnn'nnnn_ 4 0 100 100 100 400 ! >IN ; Top Bottom ;Name Offset Recognition Pattern Offset Err dam dcm units $ ! Isoschiz >Supplier Index SfcI 1 C'TryA_G 4 0 100 100 250 200 ! BfmI,BstSFI >N ;SfeI 1 C'TryA_G 4 0 100 100 0 0 ! BfmI,BstSFI,SfcI > SfiI 8 GGCCn_nnn'nGGCC -3 0 100 -6 10000 200 ! >ABCDEGIJKLMNOQRST SfoI 3 GGC'GCC 0 0 100 100 500 200 ! NarI,BbeI,EgeI,EheI,KasI,Mly113I >N ;Sfr274I 1 C'TCGA_G 4 0 100 100 0 0 ! XhoI,PaeR7I,TliI >I ;Sfr303I 4 CC_GC'GG -2 0 100 100 0 0 ! SacII,Cfr42I,KspI,SstII >I ;SfuI 2 TT'CG_AA 2 0 100 100 0 0 ! Bpu14I,BsiCI,Bsp119I,BstBI,CbiI,Csp45I,LspI,NspV >M SgfI 5 GCG_AT'CGC -2 0 100 100 0 0 ! >R SgrAI 2 Cr'CCGG_yG 4 0 100 100 5000 200 ! >MN ;SimI 2 GG'GTC_ 3 0 100 100 0 0 ! > ;SinI 1 G'GwC_C 3 0 100 100 0 0 ! AvaII,Bme18I,Eco47I,HgiEI >LR SmaI 3 CCC'GGG 0 0 100 100 10000 176 ! Cfr9I,PspAI,XmaI,XmaCI >ABCDEFGHIJKLMNOQRST ;SmiI 4 ATTT'AAAT 0 0 100 100 0 0 ! SwaI >DFI SmlI 1 C'TyrA_G 4 0 100 100 1000 200 ! >N ;SnaI 3 GTATAC 0 0 100 100 0 0 ? ! BssNAI,Bst1107I,BstZ17I > SnaBI 3 TAC'GTA 0 0 100 100 2500 176 ! BstSNI,Eco105I >ACEKLMNR SpeI 1 A'CTAG_T 4 0 100 100 2500 200 ! AclNI,BcuI >ABDEHJKLMNOQRST SphI 5 G_CATG'C -4 0 100 100 1250 200 ! BbuI,PaeI >ABCDEGHIJKLMNOQRST ;SplI 1 C'GTAC_G 4 0 100 100 0 0 ! BsiWI,Pfl23II,PspLI,SunI > SrfI 4 GCCC'GGGC 0 0 100 100 0 0 ! >EO ;Sse9I 0 'AATT_ 4 0 100 100 0 0 ! TspEI,TasI,Tsp509I >DI ;Sse232I 2 CG'CCGG_CG 4 0 100 100 0 0 ! > ;Sse8387I 6 CC_TGCA'GG -4 0 100 100 0 0 ! SbfI,SdaI >AK ;Sse8647I 2 AG'GwC_CT 3 0 100 100 0 0 ! > ;SseBI 3 AGG'CCT 0 0 100 100 0 0 ! StuI,AatI,Eco147I,Pme55I >C SspI 3 AAT'ATT 0 0 100 100 5000 200 ! >ABCDEFIJKLMNOQRS ;SspBI 1 T'GTAC_A 4 0 100 100 0 0 ! Bsp1407I,AauI,BsrGI >M ;SspD5I 13 GGTGAnnnnnnnn' 0 0 100 100 0 0 ! HphI,AsuHPI > ;SstI 5 G_AGCT'C -4 0 100 100 0 0 ! SacI,Ecl136II,EcoICRI,Psp124BI >BC ;SstII 4 CC_GC'GG -2 0 100 100 0 0 ! SacII,Cfr42I,KspI,Sfr303I >B ; Top Bottom ;Name Offset Recognition Pattern Offset Err dam dcm units $ ! Isoschiz >Supplier Index ;Sth132I 8 CCCGnnnn'nnnn_ 4 0 100 100 0 0 ! > ;StsI 15 GGATGnnnnnnnnnn'nnnn_ 4 0 100 100 0 0 ! FokI,BseGI,BstF5I > StuI 3 AGG'CCT 0 0 100 0 5000 176 ! AatI,Eco147I,Pme55I,SseBI >ABEJKLMNQRST StyI 1 C'CwwG_G 4 0 100 100 15000 220 ! BssT1I,Eco130I,EcoT14I,ErhI >BCEJMNRS ;SunI 1 C'GTAC_G 4 0 100 100 0 0 ! BsiWI,Pfl23II,PspLI >B SwaI 4 ATTT'AAAT 0 0 100 100 10000 200 ! SmiI >KMNS ;TaaI 3 AC_n'GT -1 0 100 100 0 0 ! Bst4CI >F TaiI 4 _ACGT' -4 0 100 100 0 0 ! MaeII,TscI >F TaqI 1 T'CG_A 2 0 1 100 20000 176 ! TthHB8I >ABCDEFGIJLMNOQRST ;TaqII 17 GACCGAnnnnnnnnn_nn' -2 0 100 100 0 0 ! > ;TaqII 17 CACCCAnnnnnnnnn_nn' -2 0 100 100 0 0 ! > ;TasI 0 'AATT_ 4 0 100 100 0 0 ! TspEI,Sse9I,Tsp509I >F TatI 1 w'GTAC_w 4 0 100 100 0 0 ! >F ;TauI 2 GCsGC 0 0 100 100 0 0 ? ! > TfiI 1 G'AwT_C 3 0 100 100 500 200 ! >N ;ThaI 2 CG'CG 0 0 100 100 0 0 ! AccII,Bsh1236I,BstFNI,BstUI,MvnI >B TliI 3 CTCGAG 0 0 100 100 5000 200 ? ! XhoI,PaeR7I,Sfr274I >N ;Tru1I 1 T'TA_A 2 0 100 100 0 0 ! MseI,Tru9I >F ;Tru9I 1 T'TA_A 2 0 100 100 0 0 ! MseI,Tru1I >DILMR ;TscI 4 _ACGT' -4 0 100 100 0 0 ! MaeII,TaiI >L TseI 1 G'CwG_C 3 0 100 100 375 200 ! >N Tsp45I 0 'GTsAC_ 5 0 100 100 500 220 ! >N Tsp509I 0 'AATT_ 4 0 100 100 5000 200 ! TspEI,Sse9I,TasI >N ;Tsp4CI 3 AC_n'GT -1 0 100 100 0 0 ! Bst4CI,TaaI > ;TspEI 0 'AATT_ 4 0 100 100 0 0 ! Sse9I,TasI,Tsp509I >LO TspRI 9 _nnCAsTGnn' -9 0 100 100 2500 220 ! >N Tth111I 4 GACn'n_nGTC 1 0 100 100 2000 176 ! AspI,PflFI,PsyI >ADIKNQR ;Tth111II 17 CAArCAnnnnnnnnn_nn' -2 0 100 100 0 0 ! > ;TthHB8I 1 T'CG_A 2 0 100 100 0 0 ! TaqI >AK ;UbaPI 3 CGAACG 0 0 100 100 0 0 ? ! > ; Top Bottom ;Name Offset Recognition Pattern Offset Err dam dcm units $ ! Isoschiz >Supplier Index ;UnbI 0 'GGnCC_ 5 0 100 100 0 0 ! AspS9I,BsiZI,Cfr13I,Sau96I > ;Van91I 7 CCAn_nnn'nTGG -3 0 100 100 0 0 ! PflMI,AccB7I >DFKM ;Vha464I 1 C'TTAA_G 4 0 100 100 0 0 ! AflII,BfrI,BspTI,Bst98I,MspCI >I ;VneI 1 G'TGCA_C 4 0 100 100 0 0 ! ApaLI,Alw44I >I ;VpaK11AI 0 'GGwCC_ 5 0 100 100 0 0 ! AvaII,Bme18I,Eco47I,HgiEI,SinI > ;VspI 2 AT'TA_AT 2 0 100 100 0 0 ! AseI,AsnI,PshBI >BDEFIR ;XagI 5 CCTnn'n_nnAGG 1 0 100 100 0 0 ! EcoNI >F ;XapI 1 r'AATT_y 4 0 100 100 0 0 ! ApoI,AcsI >F XbaI 1 T'CTAG_A 4 0 3 100 15000 220 ! >ABCDEFGHIJKLMNOQRST ;XceI 5 r_CATG'y -4 0 100 100 0 0 ! NspI,BstNSI >F XcmI 8 CCAnnnn_n'nnnnTGG -1 0 100 100 2500 200 ! >AN XhoI 1 C'TCGA_G 4 0 100 100 25000 220 ! PaeR7I,Sfr274I,TliI >ABCDEFGHJKLMNOQRST ;XhoII 1 r'GATC_y 4 0 100 100 0 0 ! BstX2I,BstYI,MflI >EMR XmaI 1 C'CCGG_G 4 0 100 100 1250 200 ! SmaI,Cfr9I,PspAI,XmaCI >ADINR ;XmaIII 1 C'GGCC_G 4 0 100 100 0 0 ! BseX3I,BstZI,EagI,EclXI,Eco52I >BE ;XmaCI 1 C'CCGG_G 4 0 100 100 0 0 ! SmaI,Cfr9I,PspAI,XmaI >M ;XmiI 2 GT'mk_AC 2 0 100 100 0 0 ! AccI,FblI >F XmnI 5 GAAnn'nnTTC 0 0 100 100 5000 176 ! Asp700I,MroXI >DENR ;Zsp2I 5 A_TGCA'T -4 0 100 100 0 0 ! EcoT22I,Mph1103I,NsiI,Ppu10I >I