LOCUS       PBI101TD                5349 bp    DNA     linear   SYN 06-MAY-2008
DEFINITION  GUS gene fusion vector pBI101 T-DNA region.
ACCESSION   U12639
VERSION     U12639.1  GI:2088506
KEYWORDS    pBI101; T-DNA; GUS gene fusion vector; neomycin phosphotransferase;
            beta-glucuronidase.
SOURCE      Cloning vector pBI101
  ORGANISM  Cloning vector pBI101
            other sequences; artificial sequences; vectors.
REFERENCE   1  (sites)
  AUTHORS   Jefferson,R.A., Burgess,S.M. and Hirsh,D.
  TITLE     beta-Glucuronidase from Escherichia coli as a gene-fusion marker
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 83 (22), 8447-8451 (1986)
   PUBMED   3534890
REFERENCE   2  (bases 2497 to 2556)
  AUTHORS   Jefferson,R.
  TITLE     Assaying chimeric genes in plants: the GUS gene fusion system
  JOURNAL   Plant Mol. Biol. Rep. 5, 387-405 (1987)
REFERENCE   3  (bases 1 to 5349)
  AUTHORS   Jefferson,R.A., Kavanagh,T.A. and Bevan,M.W.
  TITLE     GUS fusions: beta-glucuronidase as a sensitive and versatile gene
            fusion marker in higher plants
  JOURNAL   EMBO J. 6 (13), 3901-3907 (1987)
   PUBMED   3327686
REFERENCE   4  (bases 1 to 5349)
  AUTHORS   Wei,W. and Lindsey,K.
  TITLE     T-DNA sequence of the GUS gene fusion vector pBI101
  JOURNAL   Unpublished
REFERENCE   5  (bases 1 to 5349)
  AUTHORS   Wei,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JUL-1994) Wenbin Wei, Botany, University of
            Leicester, University Road, Leicester, LE1 7RH, UK
COMMENT     On May 14, 1997 this sequence version replaced gi:529328.
FEATURES             Location/Qualifiers
     source          1..5349
                     /organism="Cloning vector pBI101"
                     /mol_type="other DNA"
                     /db_xref="taxon:36566"
                     /note="sequence from the right border to the left border"
     misc_feature    1..2497
                     /note="pBin19 sequence, containing a neomycin
                     phosphotransferase gene driven by nos promoter"
     repeat_region   1..25
                     /note="the right border repeat"
     CDS             385..1179
                     /codon_start=1
                     /transl_table=11
                     /product="neomycin phosphotransferase"
                     /protein_id="AAC53707.1"
                     /db_xref="GI:2102680"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    2497..2537
                     /note="polylinker"
     CDS             2551..4359
                     /note="modified beta-glucuronidase gene coding region from
                     pRAJ260"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="AAC53706.1"
                     /db_xref="GI:529330"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGQQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQVHGNISPLAEATRKLDPTRPITCVNVMFCDA
                     HTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDT
                     LAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNK
                     KGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     3'UTR           4430..4682
                     /note="3'UTR of the nopaline synthase gene"
     misc_feature    4686..5349
                     /note="pBin19 sequence (see also GenBank Accession Number
                     U09365)"
     repeat_region   5325..5349
                     /note="the left border repeat"
ORIGIN      
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatca tgagcggaga attaagggag tcacgttatg acccccgccg atgacgcggg
      121 acaagccgtt ttacgtttgg aactgacaga accgcaacgt tgaaggagcc actcagccgc
      181 gggtttctgg agtttaatga gctaagcaca tacgtcagaa accattattg cgcgttcaaa
      241 agtcgcctaa ggtcactatc agctagcaaa tatttcttgt caaaaatgct ccactgacgt
      301 tccataaatt cccctcggta tccaattaga gtctcatatt cactctcaat ccaaataatc
      361 tgcaccggat ctggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
      421 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
      481 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
      541 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
      601 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
      661 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
      721 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
      781 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
      841 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
      901 aggctcaagg cgcgcatgcc cgacggcgat gatctcgtcg tgacccatgg cgatgcctgc
      961 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     1021 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     1081 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     1141 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     1201 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     1261 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     1321 gggatctcat gctggagttc ttcgcccacg ggatctctgc ggaacaggcg gtcgaaggtg
     1381 ccgatatcat tacgacagca acggccgaca agcacaacgc cacgatcctg agcgacaata
     1441 tgatcgggcc cggcgtccac atcaacggcg tcggcggcga ctgcccaggc aagaccgaga
     1501 tgcaccgcga tatcttgctg cgttcggata ttttcgtgga gttcccgcca cagacccgga
     1561 tgatccccga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg
     1621 gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca
     1681 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca
     1741 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg
     1801 tgtcatctat gttactagat cgggcctcct gtcaatgctg gcggcggctc tggtggtggt
     1861 tctggtggcg gctctgaggg tggtggctct gagggtggcg gttctgaggg tggcggctct
     1921 gagggaggcg gttccggtgg tggctctggt tccggtgatt ttgattatga aaagatggca
     1981 aacgctaata agggggctat gaccgaaaat gccgatgaaa acgcgctaca gtctgacgct
     2041 aaaggcaaac ttgattctgt cgctactgat tacggtgctg ctatcgatgg tttcattggt
     2101 gacgtttccg gccttgctaa tggtaatggt gctactggtg attttgctgg ctctaattcc
     2161 caaatggctc aagtcggtga cggtgataat tcacctttaa tgaataattt ccgtcaatat
     2221 ttaccttccc tccctcaatc ggttgaatgt cgcccttttg tctttggccc aatacgcaaa
     2281 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     2341 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     2401 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     2461 tttcacacag gaaacagcta tgaccatgat tacgccaagc ttgcatgcct gcaggtcgac
     2521 tctagaggat ccccgggtgg tcagtccctt atgttacgtc ctgtagaaac cccaacccgt
     2581 gaaatcaaaa aactcgacgg cctgtgggca ttcagtctgg atcgcgaaaa ctgtggaatt
     2641 gatcagcgtt ggtgggaaag cgcgttacaa gaaagccggg caattgctgt gccaggcagt
     2701 tttaacgatc agttcgccga tgcagatatt cgtaattatg cgggcaacgt ctggtatcag
     2761 cgcgaagtct ttataccgaa aggttgggca ggccagcgta tcgtgctgcg tttcgatgcg
     2821 gtcactcatt acggcaaagt gtgggtcaat aatcaggaag tgatggagca tcagggcggc
     2881 tatacgccat ttgaagccga tgtcacgccg tatgttattg ccgggaaaag tgtacgtatc
     2941 accgtttgtg tgaacaacga actgaactgg cagactatcc cgccgggaat ggtgattacc
     3001 gacgaaaacg gcaagaaaaa gcagtcttac ttccatgatt tctttaacta tgccggaatc
     3061 catcgcagcg taatgctcta caccacgccg aacacctggg tggacgatat caccgtggtg
     3121 acgcatgtcg cgcaagactg taaccacgcg tctgttgact ggcaggtggt ggccaatggt
     3181 gatgtcagcg ttgaactgcg tgatgcggat caacaggtgg ttgcaactgg acaaggcact
     3241 agcgggactt tgcaagtggt gaatccgcac ctctggcaac cgggtgaagg ttatctctat
     3301 gaactgtgcg tcacagccaa aagccagaca gagtgtgata tctacccgct tcgcgtcggc
     3361 atccggtcag tggcagtgaa gggccaacag ttcctgatta accacaaacc gttctacttt
     3421 actggctttg gtcgtcatga agatgcggac ttacgtggca aaggattcga taacgtgctg
     3481 atggtgcacg accacgcatt aatggactgg attggggcca actcctaccg tacctcgcat
     3541 tacccttacg ctgaagagat gctcgactgg gcagatgaac atggcatcgt ggtgattgat
     3601 gaaactgctg ctgtcggctt taacctctct ttaggcattg gtttcgaagc gggcaacaag
     3661 ccgaaagaac tgtacagcga agaggcagtc aacggggaaa ctcagcaagc gcacttacag
     3721 gcgattaaag agctgatagc gcgtgacaaa aaccacccaa gcgtggtgat gtggagtatt
     3781 gccaacgaac cggatacccg tccgcaagtg cacgggaata tttcgccact ggcggaagca
     3841 acgcgtaaac tcgacccgac gcgtccgatc acctgcgtca atgtaatgtt ctgcgacgct
     3901 cacaccgata ccatcagcga tctctttgat gtgctgtgcc tgaaccgtta ttacggatgg
     3961 tatgtccaaa gcggcgattt ggaaacggca gagaaggtac tggaaaaaga acttctggcc
     4021 tggcaggaga aactgcatca gccgattatc atcaccgaat acggcgtgga tacgttagcc
     4081 gggctgcact caatgtacac cgacatgtgg agtgaagagt atcagtgtgc atggctggat
     4141 atgtatcacc gcgtctttga tcgcgtcagc gccgtcgtcg gtgaacaggt atggaatttc
     4201 gccgattttg cgacctcgca aggcatattg cgcgttggcg gtaacaagaa agggatcttc
     4261 actcgcgacc gcaaaccgaa gtcggcggct tttctgctgc aaaaacgctg gactggcatg
     4321 aacttcggtg aaaaaccgca gcagggaggc aaacaatgaa tcaacaactc tcctggcgca
     4381 ccatcgtcgg ctacagcctc gggaattgct accgagctcg aatttccccg atcgttcaaa
     4441 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat
     4501 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt
     4561 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa
     4621 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga
     4681 tcgggaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     4741 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     4801 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgcccgctcc tttcgctttc
     4861 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     4921 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt
     4981 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     5041 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     5101 ggctattctt ttgatttata agggattttg ccgatttcgg aaccaccatc aaacaggatt
     5161 ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg
     5221 tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc ccagtacatt
     5281 aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttgtttac accacaatat
     5341 atcctgcca
//

