LOCUS       PCRII        3932 bp    DNA   circular  SYN       17-MAY-1996
DEFINITION  pCRII.
ACCESSION   PCRII0
KEYWORDS    .
SOURCE      synthetic construct.
  ORGANISM  synthetic construct
            Unclassified.
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Fristensky,B.
  TITLE     untitled
  JOURNAL   Unpublished Plant Science, University of Manitoba, Winnipeg,
            Manitoba R3T 2N2, Canada
COMMENT     PCR products generated by Taq polymerase typically contain a single
            5' protruding A. To clone these products, pCRII is cut at the EcoRI
            site and an EcoRI adaptor is added to each end to give the
            following structure:
            
            5' GAATTCGGCTT            AATTC3'
            3' CTTAA            TTCGGCTTAAG5'
            
            The 5' overhanging A will base pair with
            the one of the T's, and presumably E. coli
            will repair the site.
FEATURES             Location/Qualifiers
     source          1..3932
                     /organism="synthetic construct"
                     /plasmid=""
     rep_origin      572..986
                     /standard_name="F1"
     rep_origin      3182..3765
                     /standard_name="ColE1"
     promoter        239..255
                     /label=Sp6_promoter
     misc_feature    269..381
                     /label=MCS
     promoter        388..407
                     /label=T7_promoter
     misc_feature    1..571
                     /gene="lacZ"
                     /note="beta-galactosidase~"
     misc_feature    987..2114
                     /gene="nptII"
                     /note="Description: kanamycin resistance"
     misc_feature    2133..2992
                     /gene="AmpR"
                     /note="Description: ampicillin resistance"
BASE COUNT      915 a   1011 c   1065 g    941 t
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat
      241 ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca
      301 ctagtaacgg ccgccagtgt gctggaattc tgcagatatc catcacactg gcggccgctc
      361 gagcatgcat ctagagggcc caattcgccc tatagtgagt cgtattacaa ttcactggcc
      421 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
      481 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
      541 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
      601 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
      661 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
      721 aatcgggggc tccctttagg gttccgattt agagctttac ggcacctcga ccgcaaaaaa
      781 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
      841 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
      901 aaccctatcg cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
      961 ttaaaaaatg agctgattta acaaattcag ggcgcaaggg ctgctaaagg aaccggaaca
     1021 cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta
     1081 tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat
     1141 ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg
     1201 cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa
     1261 ggatctgatg gcgcagggga tcaagatctg atcaagagac aggatgagga tcgtttcgca
     1321 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     1381 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     1441 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     1501 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     1561 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     1621 atctcctgtc atctcgcctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     1681 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     1741 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     1801 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     1861 gcgaggatct cgtcgtgatc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     1921 gccgcttttc tggattcaac gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     1981 tagcgttgga tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     2041 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     2101 acgagttctt ctgaattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     2161 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     2221 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     2281 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     2341 taaagttctg ctatgtgata cactattatc ccgtattgac gccgggcaag agcaactcgg
     2401 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     2461 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     2521 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     2581 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     2641 cataccaaac gacgagagtg acaccacgat gcctgtagca atgccaacaa cgttgcgcaa
     2701 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actgaatgga
     2761 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     2821 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     2881 tggtaagcgc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     2941 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     3001 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     3061 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     3121 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     3181 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     3241 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     3301 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     3361 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     3421 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     3481 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     3541 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     3601 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     3661 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     3721 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     3781 cctgggcttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     3841 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     3901 gcgcagcgag tcagtgagcg aggaagcgga ag
//
