LOCUS       PI230       3205 bp    DNA   Circular  SYN       19-JUN-1995
DEFINITION  Plasmid p230, with Drr230a cDNA insert in pUC9
ACCESSION   CD9602
KEYWORDS    cysteine-rich protein; disease resistance response protein 230;
            pathogenesis-related protein.
SOURCE      Pisum sativum.
  ORGANISM  Pisum sativum
            Unclassified.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Riggleman,R.C., Fristensky,B. and Hadwiger,L.A.
  TITLE     The disease resistance response in pea is associated with increased
            levels of specific mRNAs
  JOURNAL   Plant Molecular Biology 4, 81-86 (1985)
REFERENCE   2  (bases 1 to 468)
  AUTHORS   Chiang,C.C. and Hadwiger,L.A.
  TITLE     The Fusarium solani-Induced Expression of a Pea Gene Family
            Encoding High Cysteine Content Proteins
  JOURNAL   Molecular Plant-Microbe Interactions 4, 324-331 (1991)
REFERENCE   3  (bases 1 to 3205)
  AUTHORS   Fristensky,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUN-1995) B. Fristensky, Plant Science, University of
            Manitoba, Winnipeg, MB, Canada
FEATURES             Location/Qualifiers
     source          L01578:1...468
                     /evidence= EXPERIMENTAL
                     /label=Drr230a_cDNA
                     /citation=[2]
     source          "aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa" 
                     /label=polyA_tail
                     /note= "About 50bp but precise length unknown"
     source          L09128:join(242..2665,1..245)
                     /function= "vector"
                     /evidence= EXPERIMENTAL
                     /standard_name= "pUC9"
                     /label=pUC9_Pst
                     /note= "pUC9, PstI cut"
     source          "cccccccccccccccccc"
                     /evidence= EXPERIMENTAL
                     /label=polyC-tail
                     /note= "C-tailed PstI
                     site"
     source          join(Drr230a_cDNA,polyA_tail,pUC9_Pst,polyC-tail)
                     /organism="Escherichia coli"
                     /plasmid="pI230"
                     /evidence= EXPERIMENTAL
                     /label=pI230
     mRNA            1..506
     CDS             78..296
                     /function="unknown"
                     /standard_name="pathogenesis related protein"
                     /product="disease resistance response protein"
                     /gene="DRR230-a"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MEKKSLACLSFLLLVLFVAQEIVVSEANTCENLAGSYKGVCFGG
                     CDRHCRTQEGAISGRCRDDFRCWCTKNC"
                     /citation=[2]
     mat_peptide     158..293
                     /function="unknown"
                     /note="determined by in vitro cleavage with microsomal
                     membranes"
                     /product="disease resistance response protein"
                     /gene="DRR230-a"
                     /evidence=EXPERIMENTAL
                     /codon_start=2
                     /citation=[2]
BASE COUNT      858 a    785 c    756 g    805 t
ORIGIN          
       1  gatcacacaa acacacataa cacattaagt gaagtgagtc atattaagtt
      51  tttatattca tcactactta agaagccatg gagaagaaat cactagcttg
     101  cttgtccttc ctcctcctcg ttctctttgt tgcacaagaa atagtggtga
     151  gtgaagcaaa cacatgtgag aatttggctg gttcatataa gggagtatgc
     201  ttcggtggat gtgaccgtca ctgtagaaca caagagggcg caattagcgg
     251  cagatgcagg gatgactttc gctgttggtg cactaaaaac tgttaaatcc
     301  cttttctcca acaccaacaa cacccatata taatactata atataaataa
     351  ataaacaagt gttgtttcga attctatgtg tgtactcaat atcgtgtata
     401  acgtgtttgt tatgcacttt tatcatatca tatggaataa aaagtaatca
     451  atcatttcct ttccaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     501  aaaaaaaaaa aaaaaaaatg caggtcgacg gatccccggg aattcactgg
     551  ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     601  aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     651  ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     701  ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     751  cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     801  ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     851  tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     901  atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     951  cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
    1001  cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
    1051  tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
    1101  accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
    1151  aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
    1201  gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
    1251  gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
    1301  tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
    1351  aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
    1401  gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
    1451  caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
    1501  tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
    1551  gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
    1601  gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
    1651  ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
    1701  gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
    1751  aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
    1801  tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
    1851  gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
    1901  cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
    1951  cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
    2001  actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
    2051  atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
    2101  accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
    2151  agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
    2201  gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
    2251  gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
    2301  gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
    2351  tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
    2401  ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
    2451  aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
    2501  cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
    2551  ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
    2601  ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
    2651  agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
    2701  cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
    2751  cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
    2801  gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
    2851  gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
    2901  aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
    2951  tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
    3001  cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
    3051  tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
    3101  cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
    3151  aacagctatg accatgatta cgccaagctt ggctgcaccc cccccccccc
    3201  ccccc
//

