LOCUS       PI49KSV      4258 bp    DNA   Circular  SYN       2-JUN-1997
DEFINITION  Plasmid pI49KSv - internal PCR standard
ACCESSION   MD8800
KEYWORDS    .
SOURCE      derived from recombinant construct pI49KS.
  ORGANISM  synthetic
REFERENCE   1  (sites)
  AUTHORS   Fristensky, Brian W
  TITLE     Untitled
  JOURNAL   Unpublished (1997) Plant Science, University of Manitoba
  STANDARD  full automatic
COMMENT
            This clone was made Mary DePauw in Brian Fristensky's lab,
            Experiment MD88. It contains five pUC18/Sau3A1 fragments inserted
            at the BglII site within the cDNA. Amplification using primers
            flanking this insertion gives a PCR fragment 495bp longer than
            that generated in RT-PCR from mRNA.

            Corresponding Address for Citation 1:
            Brian W Fristensky
            University of Manitoba
            Winnipeg, MB  R3T 2N2
            Canada
            Phone: 204-474-6085
            Fax:   204-261-5732
            Email: frist@cc.umanitoba.ca
FEATURES            LOCATION/QUALIFIERS
     misc_feature    X13383:1..354
                     /evidence= EXPERIMENTAL
                     /label=drr49a_cDNA_left
     misc_feature    L08752:complement(1710..1814)
                     /function= "vector"
                     /evidence= EXPERIMENTAL
                     /standard_name= "SYNPUC18V"
                     /label=pUC18_105
                     /note= "concatenated Sau3A1 digest fragments"
     misc_feature    L08752:join(1043..1300,1301..1346,
                     1823..1900,1901..1908)
                     /function= "vector"
                     /evidence= EXPERIMENTAL
                     /standard_name= "SYNPUC18V"
                     /label=pUC18_frags
                     /note= "concatenated Sau3A1 digest fragments"
     misc_feature    X13383:355..734
                     /evidence= EXPERIMENTAL
                     /label=drr49a_cDNA_right
     unsure          "aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa" 
                     /label=polyA_tail
                     /note= "About 50bp but precise length unknown"
     misc_feature    "tgcagg"
                     /evidence= EXPERIMENTAL
                     /label=Pst_to_Sal
                     /note= "pUC9 polylinker, from T-tailed PstI site to SalI"
     misc_feature    X52331:join(735..2958,1..719)
                     /function= "vector"
                     /evidence= EXPERIMENTAL
                     /standard_name= "Bluescript KSm13+"
                     /label=KSm13_H3_Sal
                     /note= "Bluescript KSm13+/HindIII/SalI cut"
     misc_feature    "agcttggctgcacccccccccccccccccc"
                     /evidence= EXPERIMENTAL
                     /label=polyC-tail
                     /note= "pUC9 polylinker from HindIII to C-tailed PstI
                     site"
     source          join(drr49a_cDNA_left,pUC18_105,pUC18_frags,
                     drr49a_cDNA_right,polyA_tail,
                     Pst_to_Sal,KSm13_H3_Sal,polyC-tail)
                     /organism="Escherichia coli"
                     /plasmid="pI49KSv"
                     /evidence= EXPERIMENTAL
                     /label=pI49KSv

BASE COUNT     1165 a    986 c   1003 g   1104 t 
ORIGIN
        1 acacaactag gcaagcaatt tcttagttct ttctcacact ttagcattat aaaacattat
       61 catcatgggt gtttttaatg ttgaagatga aatcacttct gttgtagcac ctgctatact
      121 ctacaaagct ctagttacag atgctgataa ccttactcca aaggttattg atgccatcaa
      181 aagtatcgaa attgttgaag gaaacggtgg tgctggaacc atcaaaaaac tcactttcgt
      241 tgaagatggt gaaaccaaac atgtgttgca caaagtggag ttagtagatg ttgctaactt
      301 ggcttacaac tatagcatag ttggtggtgt tggatttcca gacacagttg agaagatcct
      361 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
      421 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atccttgaga gttttcgccc
      481 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
      541 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
      601 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
      661 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
      721 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcctt tttgataatc
      781 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
      841 agatcaaagg atctcattcg aggctaaact gtctgcagga ccaaatggag gatccattgc
      901 aaagctgagt gtgaaatact tcacaaaagg tgatgctgct cctagtgaag agcaactcaa
      961 gactgacaaa gctaaggggg atggtctttt caaggctctt gagggttact gtttggctca
     1021 tcctgattac aactaaacta tataatcatc aacaagtgtg ttgttatgta tactatataa
     1081 tcatcctgtg tgcttaattt ggctgccaat gtaattttct gttttgtttt tttccttttc
     1141 ttttgttgtg gaactaaaag tgtgagattg taagtcatgt atacctctcc ttcaataaat
     1201 tataataata atttatatat atctttttta aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     1261 aaaaaaaaaa aaaaaaaaat gcaggtcgac ctcgaggggg ggcccggtac ccagcttttg
     1321 ttccctttag tgagggttaa tttcgagctt ggcgtaatca tggtcatagc tgtttcctgt
     1381 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     1441 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     1501 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     1561 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     1621 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     1681 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     1741 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     1801 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     1861 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     1921 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     1981 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     2041 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     2101 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     2161 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     2221 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     2281 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     2341 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     2401 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     2461 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     2521 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     2581 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     2641 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     2701 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     2761 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     2821 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     2881 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     2941 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     3001 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     3061 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     3121 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     3181 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     3241 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     3301 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     3361 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     3421 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     3481 ggttccgcgc acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta
     3541 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     3601 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     3661 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     3721 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     3781 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     3841 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     3901 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     3961 cagggcgcgt cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     4021 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     4081 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga attgtaatac
     4141 gactcactat agggcgaatt ggagctccac cgcggtggcg gccgctctag aactagtgga
     4201 tcccccgggc tgcaggaatt cgatatcaag cttggctgca cccccccccc cccccccc
//
