LOCUS       PCC2         5791 bp    DNA   Circular  SYN       30-JUL-1994
DEFINITION  Plasmid PCC2
ACCESSION   CC0002
KEYWORDS    .
SOURCE      recombinant construct PCC2.
  ORGANISM  synthetic
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Chiang,C.C. and Hadwiger,L.A.
  TITLE     Cloning and characterization of a disease resistance response gene
            in pea inducible by Fusarium solani
  JOURNAL   Mol. Plant Microb. Interact. 3, 78-85 (1990)
  STANDARD  full automatic
COMMENT
            Based on information from:
              Chiang & Hadwiger (1990) Mol. Plant-Microbe Interactions 3:78-85.
              Map from C. Daniels
              Experiment BF3 11 July 1990
              Experiment BF7  6 Nov  1990

              DRRG49-c genomic sequence, GenBank Accession #J03680
              The insert is a Sal1 fragment of ~3kb. The reported sequence
              only covers a ~2.3kb Sal1/BamH1 fragment. The orientation of
              the insert and the size of the unsequenced portion were 
              determined in BF3

            These data kindly submitted in computer readable form by:
            Brian W Fristensky
            Plant Science
            University of Manitoba
            Winnipeg, Manitoba R3T 2N2
            Canada
            Phone:  204-474-6085
            Email:  frist@cc.umanitoba.ca
            Fax:    204-261-5732

            Corresponding Address for Citation 1:
            Brian W Fristensky
            University of Manitoba
            Winnipeg, MB  R3T 2N2
            Canada
            Phone: 204-474-6085
            Fax:   204-261-5732
            Email: frist@cc.umanitoba.ca
FEATURES            LOCATION/QUALIFIERS
     misc_feature    J03680:2..2290
                     /evidence= EXPERIMENTAL
                     /note="insert starts at 5'end of SalI site"
                     /label=PR10_genomic
     misc_feature    poly("n",815)
                     /evidence= EXPERIMENTAL
                     /label=unseq
                     /note= "~815bp unsequenced BamHI/SalI fragment"
     misc_feature    L08752:join(263..2686,1..263)
                     /function= "vector"
                     /evidence= EXPERIMENTAL
                     /standard_name= "pUC18"
                     /label=pUC18_Sal
                     /note= "pUC18, SalI cut"
     source          join(PR10_genomic,unseq,pUC18_Sal)
                     /organism="Escherichia coli"
                     /plasmid="PCC2"
                     /evidence= EXPERIMENTAL
                     /label=PCC2
BASE COUNT        0 a      0 c      0 g      0 t 
ORIGIN
        1 tcgacacata tgtaaacagg tcggcctata gtaaatctaa taaatcctca agatatatgt
       61 aactactaat cttgatgtca acatttctcc acacatcttg atttgactca ccagtcttgt
      121 taccctagtg aagaaatcaa cgatgctttc accttcttgt tcctcactcg ttactagagt
      181 gattcaacca caccttgatt ggaaaatggt ttcacctcac aatgatttga aaagaaaaga
      241 attatgtgaa agtgaattga gaaagataag agaaattagg atttagggag aaagagaaga
      301 agattgatca aattctgcag attttctctt tgtgttttat tccaactgaa cttccattct
      361 ttttaactac tttacaacag taagttacct cgctatttat agatgatatt acttacacac
      421 ctagtaatct cgaactaatt caactaataa atactaattt ttaactttga cgaggttaac
      481 tccttcgaca actacttact tcaattgttt aagactatat tcgactatta atttatcgaa
      541 tacattcgac tttgacttta ttgaatctta catacatgct taacacaaat aattacatat
      601 ttaacttttt tttttctcca tctctcttta ctatattaaa taatgtgaaa taatataaat
      661 actgtttaac atgttttatt tcaatatccc aatactgacc tcaaactcaa aatttagaag
      721 aaaaaagaac tagcaacaac ataatttgta tgaatagacg taacctagtt tttcacaaaa
      781 taaccagtta aatttaactg tttatatatt taagtgataa tcatttatct cggattcttt
      841 actcgacaca aatgagttaa gatgagtata tgttagtttt ggtaaaaaat gtataattta
      901 gttagatata tgtttaattt gagtaggata tgttggcacg cacgaaccaa atacgtatag
      961 gaacgtggat aaaaataata ctattgacca aatgcatatc agaacgtgaa tacaaaatgg
     1021 tccagcgact tggtattaat agctatacaa tgcatcttca tttcttataa atagaacttc
     1081 aactccactc gtaaatcaca cagctaggca agcaccttct tatttatagc attataaatc
     1141 atcattatca tgggtgtttt caattttgag gaagaagcca cttccattgt agctcctgct
     1201 acacttcaca aagctctggt tacagatgct gacattctta ctccaaaggt tattgatgcc
     1261 atcaaaagta ttgaaattgt tgaaggaaac ggtggccccg gaaccatcaa gaaactcact
     1321 ttcgttgaag gtcagtatta gaatattctt ttcatgatat atcattatta ctagtattat
     1381 tgtttatgat tgtgaaatga atcaattgtg tgttgcagac ggtgaaacca agtatgtgtt
     1441 acacaaagtg gagttagtag atgatgctaa ctgggcaaac aactacagca tagttggagg
     1501 tgttggactt ccggacacag ttgagaagat ctcgtttgag gctaaattgt ctgcaggacc
     1561 aaatggagga tccattgcaa agctgagtgt gaaatattat accaaaggtg atgctattcc
     1621 tagtgaagag gaaatcaaga atggcaaagc caaaggtgaa ggtattttca aggctcttga
     1681 aggttactgt gtggctaatc ctgattacaa ctaaaaaatt taattaagtg agtgcttgtt
     1741 ttattatggt gtgttatgac acattttatt gcatctgtcg gcttaatttg ttttcttatt
     1801 tttctttctt ttccctttcc tattgttgag gaagtgtgag tttgagattg taagtcatgt
     1861 ttgtaccacg ttttaagaaa ttataataat acgtatgttc ttttttatat attttttttt
     1921 ctctagaaaa tattgttact aaaacaaaat tgttactaaa aaacatttaa aatattggta
     1981 gggatttgat accaccaatg taaaccattg aaatgaagat ccaaaacgtg actactttga
     2041 ttgttttggt aataaaataa aaaatttcaa tcacaccaaa aaaatttaat ttgtatataa
     2101 ctaaaataaa taatcttcta cagaacaata acaagataaa atacaaaaaa acacaataat
     2161 ttgtgtactc agttcgatca aaaaaatcta ctctaaaggt aagaaaaact ctgagtcatt
     2221 atacttccta ttgtgggccg accaaagtca atccaagagt ccaaccagag tcagaccaat
     2281 caaggatccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngtcgac ctgcaggcat
     3121 gcaagcttgg cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     3181 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     3241 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg
     3301 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
     3361 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     3421 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     3481 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     3541 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     3601 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     3661 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     3721 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     3781 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     3841 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     3901 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     3961 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     4021 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     4081 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     4141 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     4201 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     4261 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     4321 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     4381 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     4441 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     4501 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     4561 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     4621 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     4681 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     4741 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     4801 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     4861 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     4921 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     4981 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     5041 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     5101 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     5161 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     5221 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     5281 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     5341 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     5401 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     5461 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     5521 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     5581 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     5641 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     5701 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgaa
     5761 ttcgagctcg gtacccgggg atcctctaga g
//
