LOCUS       pFRG1        3380 bp    DNA   circular  SYN        8-SEP-1995
DEFINITION  Plasmid pFRG1. Radish Rs-AFP1 cDNA in pBluescriptIISK-
ACCESSION   PZ000000
REFERENCE   1  (1..395)
  AUTHORS   Terras,F.R.G., Eggermont,K., Kovaleva,V., Raikhel,N.V.,
            Osborn,R.W., Kester,A., Rees,S.B., Torrekens,S., Van Leuven,F.,
            Vanderleyden,J., Cammue,B.P.A. and Broekaert,W.F.
  TITLE     Small cysteine-rich antifungal proteins from radish: their role in
            host defense
  JOURNAL   Plant Cell 7, 568-573 (1995)
FEATURES               Location/Qualifiers
     source          U18557:1..395
                     /strain="ronde rode kleine witpunt"
                     /organism="Raphanus sativus"
                     /tissue_type="seed"
                     /label=Rs-AFP1_cDNA
     source          "AGCGGCCGCG"
                     /note=Pharmacia Cohesive-End Adaptor (lower strand)
                     /label=RINotI_lower
     source          X52330:join(complement(1..705),complement(706..2961))
                     /note="pBluescriptIISK-, cut at EcoRI site"
                     /label=ARBL2SKM_RI
     source          "AATTCGCGGCCGCT"
                     /note="Pharmacia Cohesive-End Adaptor (upper strand)"
                     /label=RINotI_upper
     plasmid         join(Rs-AFP1_cDNA,RINotI_lower,ARBL2SKM_RI,
                     RINotI_upper)
                     /label=pFGR1   
ORIGIN      
       1  gtttattagt gatcatggct aagtttgcgt ccatcatcgc acttcttttt
      51  gctgctcttg ttctttttgc tgctttcgaa gcaccaacaa tggtggaagc
     101  acagaagttg tgcgaaaggc caagtgggac atggtcagga gtctgtggaa
     151  acaataacgc atgcaagaat cagtgcatta accttgagaa agcacgacat
     201  ggatcttgca actatgtctt cccagctcac aagtgtatct gctactttcc
     251  ttgttaattt atcgcaaact ctttggtgaa tagtttttat gtaatttaca
     301  caaaataagt cagtgtcact atccatgagt gattttaaga catgtaccag
     351  atatgttatg ttggttcggt tatacaaata aagttttatt caccaAGCGG
     401  CCGCGaattc gatatcaagc ttatcgatac cgtcgacctc gagggggggc
     451  ccggtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     501  cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     551  atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     601  gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     651  gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     701  aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     751  aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     801  aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     851  cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     901  ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     951  ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
    1001  agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
    1051  tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
    1101  ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
    1151  tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
    1201  cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
    1251  catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
    1301  ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
    1351  tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
    1401  cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
    1451  agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
    1501  aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
    1551  ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
    1601  cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
    1651  caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
    1701  gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
    1751  accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
    1801  tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
    1851  ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
    1901  ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    1951  gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
    2001  cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
    2051  aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
    2101  tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
    2151  ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
    2201  caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    2251  aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
    2301  tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
    2351  tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
    2401  agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
    2451  aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
    2501  agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    2551  gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
    2601  tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
    2651  acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
    2701  acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
    2751  cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
    2801  cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    2851  tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
    2901  tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
    2951  taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
    3001  cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
    3051  cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
    3101  cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
    3151  tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
    3201  gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
    3251  cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
    3301  aacaaaagct ggagctccac cgcggtggcg gccgctctag aactagtgga
    3351  tcccccgggc tgcaggAATT CGCGGCCGCT
//
