LOCUS       PI176         3465 bp    DNA   Circular  SYN       30-JUL-1994
DEFINITION  Plasmid pI176
ACCESSION   BF0176
KEYWORDS    .
SOURCE      recombinant construct pI76.
  ORGANISM  synthetic
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Fristensky,B., Horovitz,D. and Hadwiger,L.A.
  TITLE     cDNA sequences for pea disease resistance response genes
  JOURNAL   Plant Mol. Biol. 11, 713-715 (1988)
  STANDARD  full automatic
COMMENT
            These data kindly submitted in computer readable form by:
            Brian W Fristensky
            Plant Science
            University of Manitoba
            Winnipeg, Manitoba R3T 2N2
            Canada
            Phone:  204-474-6085
            Email:  frist@cc.umanitoba.ca
            Fax:    204-261-5732

            Corresponding Address for Citation 1:
            Brian W Fristensky
            University of Manitoba
            Winnipeg, MB  R3T 2N2
            Canada
            Phone: 204-474-6085
            Fax:   204-261-5732
            Email: frist@cc.umanitoba.ca
FEATURES            LOCATION/QUALIFIERS
     misc_feature    M18249:1..728
                     /evidence= EXPERIMENTAL
                     /label=PR10_cDNA
     unsure          "aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa" 
                     /label=polyA-tail
                     /note= "About 50bp but precise length unknown"
     misc_feature    L09128:join(242..2665,1..245)
                     /function= "vector"
                     /evidence= EXPERIMENTAL
                     /standard_name= "pUC9"
                     /label=pUC9_Pst
                     /note= "pUC9, PstI cut"
     misc_feature    "cccccccccccccccccc"
                     /evidence= EXPERIMENTAL
                     /label=polyC-tail
                     /note= "C-tailed PstI
                     site"
     source          join(PR10_cDNA,polyA-tail,pUC9_Pst,polyC-tail)
                     /organism="Escherichia coli"
                     /plasmid="pI176"
                     /evidence= EXPERIMENTAL
                     /label=pI176
BASE COUNT        0 a      0 c      0 g      0 t 
ORIGIN
        1 tacacaacta ggcaagcaat ttcttagttc tttctcacac tttagcaata taaaacaata
       61 tcatcatggg tgtttttaat gttgaagatg aaatcacttc tgttgtagca cctgctatac
      121 tctacaaagc tctagttaca gatgctgaca ctcttactcc aaaggttatt gatgccatca
      181 aaagtatcga aattgttgaa ggaaacggag gtgctggaac catcaagaaa ctcactttcg
      241 ttgaagatgg tgaaaccaaa catgtgttgc acaaagtgga gttagtagat gttgctaact
      301 tggcttacaa ctatagcata gttggtggtg ttggatttcc agacacagtt gagaagatct
      361 catttgaggc taaactgtct gcaggaccaa atggaggatc cattgcaaag ctgagtgtga
      421 aatattacac aaaaggtgat gctgctgctc ctactgaaga gcaactcaag agtgacaaag
      481 ctaagggtga tggtcttttc aaggctcttg agcgttactg tttggctcat cctgattaca
      541 actaaactat ataatcatca acaagtgtgt gttgttatgt atactatata atcatcatgt
      601 gtgcttaatt tggctgccaa tgtaatcccc ctgttttgtt ttttcccttt cccttttctt
      661 tgtggactaa aagtgtgaga ttgtaagtca tgtatacctc tccttcaata aattataatc
      721 atttatataa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaatg
      781 caggtcgacg gatccccggg aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
      841 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
      901 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
      961 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
     1021 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     1081 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     1141 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     1201 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     1261 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     1321 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     1381 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     1441 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     1501 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     1561 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     1621 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     1681 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     1741 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     1801 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     1861 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     1921 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     1981 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     2041 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     2101 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     2161 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     2221 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     2281 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     2341 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     2401 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     2461 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2521 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     2581 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     2641 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     2701 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     2761 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     2821 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     2881 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     2941 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     3001 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     3061 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     3121 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     3181 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     3241 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     3301 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     3361 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     3421 accatgatta cgccaagctt ggctgcaccc cccccccccc ccccc
//
