LOCUS       PI76KS       3757 bp    DNA   Circular  SYN       29-JUL-1994
DEFINITION  Plasmid pI176KS
ACCESSION   RB7176
KEYWORDS    .
SOURCE      recombinant construct pI176KS.
  ORGANISM  synthetic
REFERENCE   1  (sites)
  AUTHORS   Fristensky, Brian W
  TITLE     Untitled
  JOURNAL   Unpublished (1993) Plant Science, University of Manitoba
  STANDARD  full automatic
COMMENT
            This clone was made by Robin Buell in Lee Hadwiger's lab
            September 1986.

            These data kindly submitted in computer readable form by:
            Brian W Fristensky
            Plant Science
            University of Manitoba
            Winnipeg, Manitoba R3T 2N2
            Canada
            Phone:  204-474-6085
            Email:  frist@cc.umanitoba.ca
            Fax:    204-261-5732

            Corresponding Address for Citation 1:
            Brian W Fristensky
            University of Manitoba
            Winnipeg, MB  R3T 2N2
            Canada
            Phone: 204-474-6085
            Fax:   204-261-5732
            Email: frist@cc.umanitoba.ca
FEATURES            LOCATION/QUALIFIERS
     misc_feature    M18249:1..728
                     /evidence= EXPERIMENTAL
                     /label=PR10.2_cDNA
     unsure          "aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa" 
                     /label=polyA_tail
                     /note= "About 50bp but precise length unknown"
     misc_feature    "tgcagg"
                     /evidence= EXPERIMENTAL
                     /label=Pst_to_Sal
                     /note= "pUC9 polylinker, from T-tailed PstI site to SalI"
     misc_feature    X52331:join(735..2958,1..719)
                     /function= "vector"
                     /evidence= EXPERIMENTAL
                     /standard_name= "Bluescript KSm13+"
                     /label=KSm13_H3_Sal
                     /note= "Bluescript KSm13+/HindIII/SalI cut"
     misc_feature    "agcttggctgcacccccccccccccccccc"
                     /evidence= EXPERIMENTAL
                     /label=polyC-tail
                     /note= "pUC9 polylinker from HindIII to C-tailed PstI
                     site"
     source          join(PR10.2_cDNA,polyA_tail,Pst_to_Sal,KSm13_H3_Sal,
                     polyC-tail)
                     /organism="Escherichia coli"
                     /plasmid="pI176KS"
                     /evidence= EXPERIMENTAL
                     /label=pI176KS

BASE COUNT        0 a      0 c      0 g      0 t 
ORIGIN
        1 tacacaacta ggcaagcaat ttcttagttc tttctcacac tttagcaata taaaacaata
       61 tcatcatggg tgtttttaat gttgaagatg aaatcacttc tgttgtagca cctgctatac
      121 tctacaaagc tctagttaca gatgctgaca ctcttactcc aaaggttatt gatgccatca
      181 aaagtatcga aattgttgaa ggaaacggag gtgctggaac catcaagaaa ctcactttcg
      241 ttgaagatgg tgaaaccaaa catgtgttgc acaaagtgga gttagtagat gttgctaact
      301 tggcttacaa ctatagcata gttggtggtg ttggatttcc agacacagtt gagaagatct
      361 catttgaggc taaactgtct gcaggaccaa atggaggatc cattgcaaag ctgagtgtga
      421 aatattacac aaaaggtgat gctgctgctc ctactgaaga gcaactcaag agtgacaaag
      481 ctaagggtga tggtcttttc aaggctcttg agcgttactg tttggctcat cctgattaca
      541 actaaactat ataatcatca acaagtgtgt gttgttatgt atactatata atcatcatgt
      601 gtgcttaatt tggctgccaa tgtaatcccc ctgttttgtt ttttcccttt cccttttctt
      661 tgtggactaa aagtgtgaga ttgtaagtca tgtatacctc tccttcaata aattataatc
      721 atttatataa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaatg
      781 caggtcgacc tcgagggggg gcccggtacc cagcttttgt tccctttagt gagggttaat
      841 ttcgagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
      901 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
      961 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     1021 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     1081 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     1141 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     1201 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     1261 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     1321 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     1381 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     1441 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     1501 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     1561 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     1621 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     1681 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     1741 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     1801 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     1861 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     1921 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     1981 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     2041 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     2101 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     2161 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     2221 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     2281 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     2341 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     2401 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     2461 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     2521 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     2581 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     2641 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     2701 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     2761 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     2821 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     2881 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     2941 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     3001 aaaagtgcca cctaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     3061 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     3121 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     3181 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     3241 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     3301 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     3361 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     3421 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca
     3481 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     3541 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
     3601 tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg
     3661 gagctccacc gcggtggcgg ccgctctaga actagtggat cccccgggct gcaggaattc
     3721 gatatcaagc ttggctgcac cccccccccc ccccccc 
//
