LOCUS       PI49         3471 bp    DNA   Circular  SYN       30-JUL-1994
DEFINITION  Plasmid pI49
ACCESSION   BF0049
KEYWORDS    .
SOURCE      recombinant construct pI49.
  ORGANISM  synthetic
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Fristensky,B., Horovitz,D. and Hadwiger,L.A.
  TITLE     cDNA sequences for pea disease resistance response genes
  JOURNAL   Plant Mol. Biol. 11, 713-715 (1988)
  STANDARD  full automatic
COMMENT
            These data kindly submitted in computer readable form by:
            Brian W Fristensky
            Plant Science
            University of Manitoba
            Winnipeg, Manitoba R3T 2N2
            Canada
            Phone:  204-474-6085
            Email:  frist@cc.umanitoba.ca
            Fax:    204-261-5732

            Corresponding Address for Citation 1:
            Brian W Fristensky
            University of Manitoba
            Winnipeg, MB  R3T 2N2
            Canada
            Phone: 204-474-6085
            Fax:   204-261-5732
            Email: frist@cc.umanitoba.ca
FEATURES            LOCATION/QUALIFIERS
     misc_feature    X13383:1..734
                     /evidence= EXPERIMENTAL
                     /label=PR10_cDNA
     unsure          "aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa" 
                     /label=polyA-tail
                     /note= "About 50bp but precise length unknown"
     misc_feature    L09128:join(242..2665,1..245)
                     /function= "vector"
                     /evidence= EXPERIMENTAL
                     /standard_name= "pUC9"
                     /label=pUC9_Pst
                     /note= "pUC9, PstI cut"
     misc_feature    "cccccccccccccccccc"
                     /evidence= EXPERIMENTAL
                     /label=polyC-tail
                     /note= "C-tailed PstI
                     site"
     source          join(PR10_cDNA,polyA-tail,pUC9_Pst,polyC-tail)
                     /organism="Escherichia coli"
                     /plasmid="pI49"
                     /evidence= EXPERIMENTAL
                     /label=pI49
BASE COUNT        0 a      0 c      0 g      0 t 
ORIGIN
        1 acacaactag gcaagcaatt tcttagttct ttctcacact ttagcattat aaaacattat
       61 catcatgggt gtttttaatg ttgaagatga aatcacttct gttgtagcac ctgctatact
      121 ctacaaagct ctagttacag atgctgataa ccttactcca aaggttattg atgccatcaa
      181 aagtatcgaa attgttgaag gaaacggtgg tgctggaacc atcaaaaaac tcactttcgt
      241 tgaagatggt gaaaccaaac atgtgttgca caaagtggag ttagtagatg ttgctaactt
      301 ggcttacaac tatagcatag ttggtggtgt tggatttcca gacacagttg agaagatctc
      361 attcgaggct aaactgtctg caggaccaaa tggaggatcc attgcaaagc tgagtgtgaa
      421 atacttcaca aaaggtgatg ctgctcctag tgaagagcaa ctcaagactg acaaagctaa
      481 gggggatggt cttttcaagg ctcttgaggg ttactgtttg gctcatcctg attacaacta
      541 aactatataa tcatcaacaa gtgtgttgtt atgtatacta tataatcatc ctgtgtgctt
      601 aatttggctg ccaatgtaat tttctgtttt gtttttttcc ttttcttttg ttgtggaact
      661 aaaagtgtga gattgtaagt catgtatacc tctccttcaa taaattataa taataattta
      721 tatatatctt ttttaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
      781 aaaatgcagg tcgacggatc cccgggaatt cactggccgt cgttttacaa cgtcgtgact
      841 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
      901 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
      961 gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     1021 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     1081 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     1141 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     1201 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     1261 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     1321 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     1381 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     1441 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     1501 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     1561 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     1621 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     1681 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1741 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1801 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1861 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     1921 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     1981 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     2041 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     2101 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     2161 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     2221 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     2281 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     2341 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     2401 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     2461 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     2521 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     2581 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     2641 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     2701 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2761 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2821 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2881 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2941 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     3001 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     3061 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     3121 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     3181 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     3241 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     3301 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     3361 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     3421 gctatgacca tgattacgcc aagcttggct gcaccccccc cccccccccc c
//
