LOCUS       PI49KS       3769 bp    DNA   Circular  SYN       29-JUL-1994
DEFINITION  Plasmid pI49KS
ACCESSION   RB7049
KEYWORDS    .
SOURCE      recombinant construct pI49KS.
  ORGANISM  synthetic
REFERENCE   1  (sites)
  AUTHORS   Fristensky, Brian W
  TITLE     Untitled
  JOURNAL   Unpublished (1993) Plant Science, University of Manitoba
  STANDARD  full automatic
COMMENT
            This clone was made by Robin Buell in Lee Hadwiger's lab
            September 1986.

            These data kindly submitted in computer readable form by:
            Brian W Fristensky
            Plant Science
            University of Manitoba
            Winnipeg, Manitoba R3T 2N2
            Canada
            Phone:  204-474-6085
            Email:  frist@cc.umanitoba.ca
            Fax:    204-261-5732

            Corresponding Address for Citation 1:
            Brian W Fristensky
            University of Manitoba
            Winnipeg, MB  R3T 2N2
            Canada
            Phone: 204-474-6085
            Fax:   204-261-5732
            Email: frist@cc.umanitoba.ca
FEATURES            LOCATION/QUALIFIERS
     misc_feature    X13383:1..734
                     /evidence= EXPERIMENTAL
                     /label=drr49a_cDNA
     unsure          "aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa" 
                     /label=polyA_tail
                     /note= "About 50bp but precise length unknown"
     misc_feature    "tgcagg"
                     /evidence= EXPERIMENTAL
                     /label=Pst_to_Sal
                     /note= "pUC9 polylinker, from T-tailed PstI site to SalI"
     misc_feature    X52331:join(735..2958,1..719)
                     /function= "vector"
                     /evidence= EXPERIMENTAL
                     /standard_name= "Bluescript KSm13+"
                     /label=KSm13_H3_Sal
                     /note= "Bluescript KSm13+/HindIII/SalI cut"
     misc_feature    "agcttggctgcacccccccccccccccccc"
                     /evidence= EXPERIMENTAL
                     /label=polyC-tail
                     /note= "pUC9 polylinker from HindIII to C-tailed PstI
                     site"
     source          join(drr49a_cDNA,polyA_tail,Pst_to_Sal,KSm13_H3_Sal,
                     polyC-tail)
                     /organism="Escherichia coli"
                     /plasmid="pI49KS"
                     /evidence= EXPERIMENTAL
                     /label=pI49KS

BASE COUNT     1019 a    878 c    902 g    970 t 
ORIGIN
        1 acacaactag gcaagcaatt tcttagttct ttctcacact ttagcattat aaaacattat 
       61 catcatgggt gtttttaatg ttgaagatga aatcacttct gttgtagcac ctgctatact 
      121 ctacaaagct ctagttacag atgctgataa ccttactcca aaggttattg atgccatcaa 
      181 aagtatcgaa attgttgaag gaaacggtgg tgctggaacc atcaaaaaac tcactttcgt 
      241 tgaagatggt gaaaccaaac atgtgttgca caaagtggag ttagtagatg ttgctaactt 
      301 ggcttacaac tatagcatag ttggtggtgt tggatttcca gacacagttg agaagatctc 
      361 attcgaggct aaactgtctg caggaccaaa tggaggatcc attgcaaagc tgagtgtgaa 
      421 atacttcaca aaaggtgatg ctgctcctag tgaagagcaa ctcaagactg acaaagctaa 
      481 gggggatggt cttttcaagg ctcttgaggg ttactgtttg gctcatcctg attacaacta 
      541 aactatataa tcatcaacaa gtgtgttgtt atgtatacta tataatcatc ctgtgtgctt 
      601 aatttggctg ccaatgtaat tttctgtttt gtttttttcc ttttcttttg ttgtggaact 
      661 aaaagtgtga gattgtaagt catgtatacc tctccttcaa taaattataa taataattta 
      721 tatatatctt ttttaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 
      781 aaaatgcagg tcgacctcga gggggggccc ggtacccagc ttttgttccc tttagtgagg 
      841 gttaattccg agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 
      901 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 
      961 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 
     1021 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 
     1081 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 
     1141 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 
     1201 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 
     1261 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 
     1321 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 
     1381 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 
     1441 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 
     1501 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 
     1561 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 
     1621 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 
     1681 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 
     1741 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 
     1801 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 
     1861 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 
     1921 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 
     1981 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 
     2041 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 
     2101 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 
     2161 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 
     2221 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 
     2281 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 
     2341 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 
     2401 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 
     2461 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 
     2521 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 
     2581 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 
     2641 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 
     2701 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 
     2761 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 
     2821 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 
     2881 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 
     2941 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 
     3001 tccccgaaaa gtgccacctg ggaaattgta aacgttaata ttttgttaaa attcgcgtta 
     3061 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat 
     3121 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca 
     3181 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc 
     3241 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta 
     3301 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg 
     3361 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg 
     3421 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg 
     3481 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg 
     3541 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca 
     3601 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta 
     3661 tagggcgaat tggagctcca ccgcggtggc ggccgctcta gaactagtgg atcccccggg 
     3721 ctgcaggaat tcgatatcaa gcttggctgc accccccccc ccccccccc 
//
