LOCUS       FLVA3         358 bp    DNA                       15-JUL-1996
DEFINITION  Pisum fulvum pathogenesis-related protein PR10 (Ypr10.Pi.ful.1)
            gene, partial cds.
ACCESSION   BFFLVA3
KEYWORDS    .
SOURCE      Pisum fulvum.
  ORGANISM  Pisum fulvum
            accession 706.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Tewari,S., Brown,S.M., Kenyon,P. and Fristensky,B.W. Dr.
  TITLE     Plant defense multigene families II. Differential expression
            patterns of PR10 multigene family members diverges rapidly among
            Pisum species
  JOURNAL   Unpublished Plant Science, University of Manitoba, Winnipeg,
            Manitoba R3T 2N2, Canada
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Pisum fulvum"
                     /note="PCR product amplified from P. fulvum genomic DNA
                     using primers oS49a+4 (5'ggtggtgctggaaccatcaaa3')
                     and oS49a-5 (5'atcccccttagctttgtcagt3') and cloned into TA
                     cloning site of Invitrogen vector pCRII to create plasmid
                     pflvA3"
     primer_bind     complement(1..21)
                     /gene="Ypr10.Pi.ful.1"
                     /note="binds oS49a+4"
     mRNA            join(<1..40,117..>358)
                     /gene="Ypr10.Pi.ful.1"
     CDS             join(<1..40,117..>358)
                     /gene="Ypr10.Pi.ful.1"
                     /note="Method: conceptual translation supplied by author."
                     /codon_start=1
                     /translation="GGAGTIKKLTFVEDGEPKHVLHKVELVDVANLAYNYSIVGGVGF
                     PDTVEKISFEAKLSAGPNGGSIAKLSVKYYTKGDAAPTEEQLKTDKAKGD"
     exon            <1..40
                     /gene="Ypr10.Pi.ful.1"
                     /number=1
     intron          41..116
                     /gene="Ypr10.Pi.ful.1"
     exon            117..>358
                     /gene="Ypr10.Pi.ful.1"
                     /number=2
     primer_bind     338..358
                     /gene="Ypr10.Pi.ful.1"
                     /note="binds oS49a-5"
BASE COUNT      114 a     59 c     84 g    101 t
ORIGIN
        1 ggtggtgctg gaaccatcaa aaaactcact ttcgttgaag gtcagtacaa atttatacat
       61 gtgttacttg aatatgctct caatatataa aattttaatt gcaattttat gtgcagatgg
      121 tgaacccaag catgtgttgc acaaagtgga gttagtagat gttgctaact tggcttacaa
      181 ctatagcata gttggtggtg ttggatttcc agacacagtt gagaagatct cattcgaggc
      241 taaactgtct gcaggaccaa atggaggatc cattgcaaag ctgagtgtga aatattacac
      301 aaaaggtgat gctgctccta ctgaagagca actcaagact gacaaagcta agggggat
//
