LOCUS       FLVB9         564 bp    DNA                       15-JUL-1996
DEFINITION  Pisum fulvum pathogenesis-related protein PR10 (Ypr10.Pi.flv.2)
            gene, partial cds.
ACCESSION   BFFLVB9
KEYWORDS    .
SOURCE      Pisum fulvum.
  ORGANISM  Pisum fulvum
            accession 706.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Tewari,S., Brown,S.M., Kenyon,P. and Fristensky,B.W.
  TITLE     Plant defense multigene families II. Differential expression
            patterns of PR10 multigene family members diverges rapidly among
            Pisum species
  JOURNAL   Unpublished Plant Science, University of Manitoba, Winnipeg,
            Manitoba R3T 2N2, Canada
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Pisum fulvum"
                     /note="PCR product amplified from P. fulvum genomic DNA
                     using primers oC49+1 (5'yawtityatcatgggtgt3') and oC49-6
                     (5'tttagttgtaatcaggat3') and cloned into TA cloning site
                     of Invitrogen vector pCRII to create plasmid pflvB9"
     exon            <1..191
                     /gene="Ypr10.Pi.ful.2"
                     /number=1
     mRNA            join(<1..191,268..>564)
                     /gene="Ypr10.Pi.ful.2"
     primer_bind     complement(1..18)
                     /gene="Ypr10.Pi.ful.2"
                     /note="binds oC49+1"
     CDS             join(11..191,268..560)
                     /gene="Ypr10.Pi.ful.2"
                     /note="Method: conceptual translation supplied by author."
                     /codon_start=1
                     /translation="MGVFNVEDEITSVVAPAILYKALVTDADTLTPKVIDAIKSIEIV
                     EGNGGAGTIKKLTFVEDGETKHVLHKVELVDLANLAYNYSIVGGVGFPDTVEKISFEA
                     KLSAGPNGGSIAKLSVKYYTKGDAAPTEEQLKTDKAKGDGLFKALEGCCLAHPDYN"
     intron          192..267
                     /gene="Ypr10.Pi.ful.2"
     exon            268..>564
                     /gene="Ypr10.Pi.ful.2"
                     /number=2
     primer_bind     547..564
                     /gene="Ypr10.Pi.ful.2"
                     /note="binds oC49-6"
BASE COUNT      174 a     96 c    122 g    172 t
ORIGIN
        1 tattgtcatc atgggtgttt ttaatgttga agatgaaatc acttctgttg tagcacctgc
       61 tatactctac aaagctctag ttacagatgc tgacactctt actccaaagg ttattgatgc
      121 catcaaaagt atcgaaattg ttgaaggaaa cggtggtgct ggaaccataa aaaaactcac
      181 tttcgttgaa ggtcagtaca aatttataca tgtgttactt gaatatgctc tcaatatata
      241 aaattttaat tgcaatttta tgtgcagatg gtgaaaccaa gcatgtcttg cacaaagtgg
      301 agttagtaga tcttgctaac ttggcttaca actatagcat agttggtggt gttggatttc
      361 cagacacagt tgagaagatc tcatttgagg ctaaactgtc tgcaggacca aatggaggat
      421 ccattgcaaa gctgagtgtg aaatattaca caaaaggtga tgctgctcct actgaagagc
      481 aactcaagac tgacaaagct aagggggatg gtcttttcaa agctcttgag ggttgctgtt
      541 tggctcatcc tgattacaac taaa
//
