<?xml version="1.0" encoding="utf-8"?>
<!DOCTYPE TS>
<TS version="2.1" language="en" sourcelanguage="en">
<context>
    <name>GTest</name>
    <message>
        <source>Contradiction in primer_task definition</source>
        <translation type="vanished">Contradiction in primer_task definition</translation>
    </message>
    <message>
        <source>Unrecognized PRIMER_TASK</source>
        <translation type="vanished">Unrecognized PRIMER_TASK</translation>
    </message>
    <message>
        <source>Illegal TARGET value: %1</source>
        <translation type="vanished">Illegal TARGET value: %1</translation>
    </message>
    <message>
        <source>Illegal PRIMER_DEFAULT_PRODUCT value: %1</source>
        <translation type="vanished">Illegal PRIMER_DEFAULT_PRODUCT value: %1</translation>
    </message>
    <message>
        <source>Illegal PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION value: %1</source>
        <translation type="vanished">Illegal PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION value: %1</translation>
    </message>
    <message>
        <source>Illegal INCLUDED_REGION value: %1</source>
        <translation type="vanished">Illegal INCLUDED_REGION value: %1</translation>
    </message>
    <message>
        <source>Illegal EXCLUDED_REGION value: %1</source>
        <translation type="vanished">Illegal EXCLUDED_REGION value: %1</translation>
    </message>
    <message>
        <source>Missing SEQUENCE tag</source>
        <translation type="vanished">Missing SEQUENCE tag</translation>
    </message>
    <message>
        <source>Error in sequence quality data</source>
        <translation type="vanished">Error in sequence quality data</translation>
    </message>
    <message>
        <source>Sequence quality data missing</source>
        <translation type="vanished">Sequence quality data missing</translation>
    </message>
    <message>
        <source>PRIMER_PAIRS_NUMBER is incorrect. Expected:%2, but Actual:%3</source>
        <translation type="vanished">PRIMER_PAIRS_NUMBER is incorrect. Expected:%2, but Actual:%3</translation>
    </message>
    <message>
        <source>%1 is incorrect. Expected:%2,%3, but Actual:NULL</source>
        <translation type="vanished">%1 is incorrect. Expected:%2,%3, but Actual:NULL</translation>
    </message>
    <message>
        <source>%1 is incorrect. Expected:NULL, but Actual:%2,%3</source>
        <translation type="vanished">%1 is incorrect. Expected:NULL, but Actual:%2,%3</translation>
    </message>
    <message>
        <source>%1 is incorrect. Expected:%2,%3, but Actual:%4,%5</source>
        <translation type="vanished">%1 is incorrect. Expected:%2,%3, but Actual:%4,%5</translation>
    </message>
    <message>
        <source>%1 is incorrect. Expected:%2, but Actual:%3</source>
        <translation type="vanished">%1 is incorrect. Expected:%2, but Actual:%3</translation>
    </message>
</context>
<context>
    <name>Primer3Dialog</name>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="33"/>
        <source>Main</source>
        <translation>Main</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="41"/>
        <source>Excluded regions</source>
        <translation>Excluded regions</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="48"/>
        <source>Targets</source>
        <translation>Targets</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="55"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Primer oligos may not overlap any region specified in this tag. The associated value must be a space-separated list of&lt;/p&gt;&lt;p&gt;&lt;span style=&quot; font-weight:600;&quot;&gt;start,length&lt;/span&gt;&lt;/p&gt;&lt;p&gt;pairs where start is the index of the first base of the excluded region, and length is its length. This tag is useful for tasks such as excluding regions of low sequence quality or for excluding regions containing repetitive elements such as ALUs or LINEs.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Primer oligos may not overlap any region specified in this tag. The associated value must be a space-separated list of&lt;/p&gt;&lt;p&gt;&lt;span style=&quot; font-weight:600;&quot;&gt;start,length&lt;/span&gt;&lt;/p&gt;&lt;p&gt;pairs where start is the index of the first base of the excluded region, and length is its length. This tag is useful for tasks such as excluding regions of low sequence quality or for excluding regions containing repetitive elements such as ALUs or LINEs.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="62"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;If one or more Targets is specified then a legal primer pair must flank at least one of them. A Target might be a simple sequence repeat site (for example a CA repeat) or a single-base-pair polymorphism. The value should be a space-separated list of &lt;/p&gt;&lt;p&gt;&lt;span style=&quot; font-weight:600;&quot;&gt;start,length&lt;/span&gt;&lt;/p&gt;&lt;p&gt;pairs where start is the index of the first base of a Target, and length is its length.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;If one or more Targets is specified then a legal primer pair must flank at least one of them. A Target might be a simple sequence repeat site (for example a CA repeat) or a single-base-pair polymorphism. The value should be a space-separated list of &lt;/p&gt;&lt;p&gt;&lt;span style=&quot; font-weight:600;&quot;&gt;start,length&lt;/span&gt;&lt;/p&gt;&lt;p&gt;pairs where start is the index of the first base of a Target, and length is its length.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="106"/>
        <source>Product size ranges</source>
        <translation>Product size ranges</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="113"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;A list of product size ranges, for example:&lt;/p&gt;&lt;p&gt;&lt;span style=&quot;font-weight:600;&quot;&gt; 150-250 100-300 301-400 &lt;/span&gt;&lt;/p&gt;&lt;p&gt;Primer3 first tries to pick primers in the first range. If that is not possible, it goes to the next range and tries again. It continues in this way until it has either picked all necessary primers or until there are no more ranges. For technical reasons this option makes much lighter computational demands than the Product Size option.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;A list of product size ranges, for example:&lt;/p&gt;&lt;p&gt;&lt;span style=&quot;font-weight:600;&quot;&gt; 150-250 100-300 301-400 &lt;/span&gt;&lt;/p&gt;&lt;p&gt;Primer3 first tries to pick primers in the first range. If that is not possible, it goes to the next range and tries again. It continues in this way until it has either picked all necessary primers or until there are no more ranges. For technical reasons this option makes much lighter computational demands than the Product Size option.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="120"/>
        <source>Mispriming/Repeat library</source>
        <translation>Mispriming/Repeat library</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="130"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;This selection indicates what mispriming library (if any) Primer3 should use to screen for interspersed repeats or for other sequence to avoid as a location for primers.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;This selection indicates what mispriming library (if any) Primer3 should use to screen for interspersed repeats or for other sequence to avoid as a location for primers.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="148"/>
        <source>Number to return</source>
        <translation>Number to return</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="155"/>
        <source>Max repeat mispriming</source>
        <translation>Max repeat mispriming</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="162"/>
        <source>Max template mispriming</source>
        <translation>Max template mispriming</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="169"/>
        <source>Max 3&apos; stability</source>
        <translation>Max 3&apos; stability</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="176"/>
        <source>Pair max repeat mispriming</source>
        <translation>Pair max repeat mispriming</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="183"/>
        <source>Pair max template mispriming</source>
        <translation>Pair max template mispriming</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="235"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;The maximum number of primer pairs to return.&lt;/p&gt;&lt;p&gt;Primer pairs returned are sorted by their &amp;quot;quality&amp;quot;, in other words by the value of the objective function (where a lower number indicates a better primer pair).&lt;/p&gt;&lt;p&gt;Caution: setting this parameter to a large value will increase running time.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;The maximum number of primer pairs to return.&lt;/p&gt;&lt;p&gt;Primer pairs returned are sorted by their &amp;quot;quality&amp;quot;, in other words by the value of the objective function (where a lower number indicates a better primer pair).&lt;/p&gt;&lt;p&gt;Caution: setting this parameter to a large value will increase running time.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="254"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;The maximum stability for the last five 3&apos; bases of a left or right primer.&lt;/p&gt;&lt;p&gt;Bigger numbers mean more stable 3&apos; ends. The value is the maximum delta G (kcal/mol) for duplex disruption for the five 3&apos; bases as calculated using the Nearest-Neighbor parameter values specified by the option of &apos;Table of thermodynamic parameters&apos;.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;The maximum stability for the last five 3&apos; bases of a left or right primer.&lt;/p&gt;&lt;p&gt;Bigger numbers mean more stable 3&apos; ends. The value is the maximum delta G (kcal/mol) for duplex disruption for the five 3&apos; bases as calculated using the Nearest-Neighbor parameter values specified by the option of &apos;Table of thermodynamic parameters&apos;.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="270"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;The maximum allowed weighted similarity with any sequence in Mispriming Library.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;The maximum allowed weighted similarity with any sequence in Mispriming Library.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="286"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;The maximum allowed sum of similarities of a primer pair (one similarity for each primer) with any single sequence in Mispriming Library. &lt;/p&gt;&lt;p&gt;Library sequence weights are not used in computing the sum of similarities.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;The maximum allowed sum of similarities of a primer pair (one similarity for each primer) with any single sequence in Mispriming Library. &lt;/p&gt;&lt;p&gt;Library sequence weights are not used in computing the sum of similarities.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="302"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;The maximum allowed similarity to ectopic sites in the sequence from which you are designing the primers.&lt;/p&gt;&lt;p&gt;The scoring system is the same as used for Max Mispriming, except that an ambiguity code is never treated as a consensus.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;The maximum allowed similarity to ectopic sites in the sequence from which you are designing the primers.&lt;/p&gt;&lt;p&gt;The scoring system is the same as used for Max Mispriming, except that an ambiguity code is never treated as a consensus.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="318"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;The maximum allowed summed similarity of both primers to ectopic sites in the sequence from which you are designing the primers.&lt;/p&gt;&lt;p&gt;The scoring system is the same as used for Max Mispriming, except that an ambiguity code is never treated as a consensus.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;The maximum allowed summed similarity of both primers to ectopic sites in the sequence from which you are designing the primers.&lt;/p&gt;&lt;p&gt;The scoring system is the same as used for Max Mispriming, except that an ambiguity code is never treated as a consensus.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="328"/>
        <source>Start codon position</source>
        <translation>Start codon position</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="335"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Index of the first base of a start codon.&lt;/p&gt;&lt;p&gt;This parameter allows Primer3 to select primer pairs to create in-frame amplicons e.g. to create a template for a fusion protein. Primer3 will attempt to select an in-frame left primer, ideally starting at or to the left of the start codon, or to the right if necessary. Negative values of this parameter are legal if the actual start codon is to the left of available sequence. If this parameter is non-negative Primer3 signals an error if the codon at the position specified by this parameter is not an ATG. A value less than or equal to -10^6 indicates that Primer3 should ignore this parameter. Primer3 selects the position of the right primer by scanning right from the left primer for a stop codon. Ideally the right primer will end at or after the stop codon.&lt;/p&gt;&lt;p&gt;This parameter should be considered EXPERIMENTAL. Please check the output carefully; some erroneous inputs might cause an error in Primer3.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Index of the first base of a start codon.&lt;/p&gt;&lt;p&gt;This parameter allows Primer3 to select primer pairs to create in-frame amplicons e.g. to create a template for a fusion protein. Primer3 will attempt to select an in-frame left primer, ideally starting at or to the left of the start codon, or to the right if necessary. Negative values of this parameter are legal if the actual start codon is to the left of available sequence. If this parameter is non-negative Primer3 signals an error if the codon at the position specified by this parameter is not an ATG. A value less than or equal to -10^6 indicates that Primer3 should ignore this parameter. Primer3 selects the position of the right primer by scanning right from the left primer for a stop codon. Ideally the right primer will end at or after the stop codon.&lt;/p&gt;&lt;p&gt;This parameter should be considered EXPERIMENTAL. Please check the output carefully; some erroneous inputs might cause an error in Primer3.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="395"/>
        <source>Pick left primer</source>
        <translation>Pick left primer</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="408"/>
        <source>or use left primer below</source>
        <translation>or use left primer below</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="425"/>
        <source>Pick hybridization probe (internal oligo)</source>
        <translation>Pick hybridization probe (internal oligo)</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="438"/>
        <source>or use oligo below</source>
        <translation>or use oligo below</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="458"/>
        <source>Pick right primer</source>
        <translation>Pick right primer</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="471"/>
        <source>or use right primer below (5&apos; to 3&apos; on opposite strand)</source>
        <translation>or use right primer below (5&apos; to 3&apos; on opposite strand)</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="501"/>
        <source>General Settings</source>
        <translation>General Settings</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="504"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;This section covers such settings as primer size, temperature, GC and other general settings.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;This section covers such settings as primer size, temperature, GC and other general settings.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="512"/>
        <source>Primer size</source>
        <translation>Primer size</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="519"/>
        <location filename="../src/Primer3Dialog.ui" line="547"/>
        <location filename="../src/Primer3Dialog.ui" line="575"/>
        <location filename="../src/Primer3Dialog.ui" line="603"/>
        <source>Min</source>
        <translation>Min</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="526"/>
        <location filename="../src/Primer3Dialog.ui" line="554"/>
        <location filename="../src/Primer3Dialog.ui" line="582"/>
        <location filename="../src/Primer3Dialog.ui" line="610"/>
        <source>Opt</source>
        <translation>Opt</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="533"/>
        <location filename="../src/Primer3Dialog.ui" line="561"/>
        <location filename="../src/Primer3Dialog.ui" line="589"/>
        <location filename="../src/Primer3Dialog.ui" line="617"/>
        <source>Max</source>
        <translation>Max</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="540"/>
        <source>Primer Tm</source>
        <translation>Primer Tm</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="734"/>
        <location filename="../src/Primer3Dialog.ui" line="747"/>
        <location filename="../src/Primer3Dialog.ui" line="760"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Minimum, Optimum, and Maximum lengths (in bases) of a primer oligo.&lt;/p&gt;&lt;p&gt;Primer3 will not pick primers shorter than Min or longer than Max, and with default arguments will attempt to pick primers close with size close to Opt.&lt;/p&gt;&lt;p&gt;Min cannot be smaller than 1. Max cannot be larger than 36. (This limit is governed by maximum oligo size for which melting-temperature calculations are valid.)&lt;/p&gt;&lt;p&gt;Min cannot be greater than Max.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Minimum, Optimum, and Maximum lengths (in bases) of a primer oligo.&lt;/p&gt;&lt;p&gt;Primer3 will not pick primers shorter than Min or longer than Max, and with default arguments will attempt to pick primers close with size close to Opt.&lt;/p&gt;&lt;p&gt;Min cannot be smaller than 1. Max cannot be larger than 36. (This limit is governed by maximum oligo size for which melting-temperature calculations are valid.)&lt;/p&gt;&lt;p&gt;Min cannot be greater than Max.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="773"/>
        <location filename="../src/Primer3Dialog.ui" line="789"/>
        <location filename="../src/Primer3Dialog.ui" line="805"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Minimum, Optimum, and Maximum melting temperatures (Celsius) for a primer oligo.&lt;/p&gt;&lt;p&gt;Primer3 will not pick oligos with temperatures smaller than Min or larger than Max, and with default conditions will try to pick primers with melting temperatures close to Opt.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Minimum, Optimum, and Maximum melting temperatures (Celsius) for a primer oligo.&lt;/p&gt;&lt;p&gt;Primer3 will not pick oligos with temperatures smaller than Min or larger than Max, and with default conditions will try to pick primers with melting temperatures close to Opt.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="821"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Minimum, Optimum, and Maximum percentage of Gs and Cs in any primer or oligo.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;
</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Minimum, Optimum, and Maximum percentage of Gs and Cs in any primer or oligo.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="838"/>
        <location filename="../src/Primer3Dialog.ui" line="869"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Minimum, Optimum, and Maximum percentage of Gs and Cs in any primer or oligo.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Minimum, Optimum, and Maximum percentage of Gs and Cs in any primer or oligo.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="848"/>
        <location filename="../src/Primer3Dialog.ui" line="855"/>
        <location filename="../src/Primer3Dialog.ui" line="862"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;The minimum, optimum, and maximum melting temperature of the amplicon.&lt;/p&gt;&lt;p&gt;Primer3 will not pick a product with melting temperature less than min or greater than max. If Opt is supplied and the Penalty Weights for Product Size are non-0 Primer3 will attempt to pick an amplicon with melting temperature close to Opt.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;The minimum, optimum, and maximum melting temperature of the amplicon.&lt;/p&gt;&lt;p&gt;Primer3 will not pick a product with melting temperature less than min or greater than max. If Opt is supplied and the Penalty Weights for Product Size are non-0 Primer3 will attempt to pick an amplicon with melting temperature close to Opt.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="954"/>
        <source>Max Tm difference</source>
        <translation>Max Tm difference</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="967"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Maximum acceptable (unsigned) difference between the melting temperatures of the left and right primers.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Maximum acceptable (unsigned) difference between the melting temperatures of the left and right primers.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="977"/>
        <source>Table of thermodynamic parameters</source>
        <translation>Table of thermodynamic parameters</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="987"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Specifies details of melting temperature calculation.&lt;/p&gt;&lt;p&gt;First method uses the table of thermodynamic parameters from the paper [Breslauer KJ, et. al (1986), Proc Natl Acad Sci 83:4746-50 http://dx.doi.org/10.1073/pnas.83.11.3746]&lt;/p&gt;&lt;p&gt;Second method (recommended) was suggested in the paper [SantaLucia JR (1998), Proc Natl Acad Sci 95:1460-65 http://dx.doi.org/10.1073/pnas.95.4.1460].&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Specifies details of melting temperature calculation.&lt;/p&gt;&lt;p&gt;First method uses the table of thermodynamic parameters from the paper [Breslauer KJ, et. al (1986), Proc Natl Acad Sci 83:4746-50 http://dx.doi.org/10.1073/pnas.83.11.3746]&lt;/p&gt;&lt;p&gt;Second method (recommended) was suggested in the paper [SantaLucia JR (1998), Proc Natl Acad Sci 95:1460-65 http://dx.doi.org/10.1073/pnas.95.4.1460].&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
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        <source>Breslauer et. al 1986</source>
        <translation>Breslauer et. al 1986</translation>
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        <location filename="../src/Primer3Dialog.ui" line="991"/>
        <location filename="../src/Primer3Dialog.ui" line="1099"/>
        <source>SantaLucia 1998</source>
        <translation>SantaLucia 1998</translation>
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        <source>Primer Designer</source>
        <translation>Primer Designer</translation>
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        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
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&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;Primer oligos may not overlap any region specified in this tag. The associated value must be a space-separated list of&lt;/p&gt;
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&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;pairs where start is the index of the first base of the excluded region, and length is its length. This tag is useful for tasks such as excluding regions of low sequence quality or for excluding regions containing repetitive elements such as ALUs or LINEs.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
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&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;Primer oligos may not overlap any region specified in this tag. The associated value must be a space-separated list of&lt;/p&gt;
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&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-weight:600;&quot;&gt;start,length &lt;/span&gt;&lt;/p&gt;
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&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;pairs where start is the index of the first base of the excluded region, and length is its length. This tag is useful for tasks such as excluding regions of low sequence quality or for excluding regions containing repetitive elements such as ALUs or LINEs.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
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&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;If one or more Targets is specified then a legal primer pair must flank at least one of them. A Target might be a simple sequence repeat site (for example a CA repeat) or a single-base-pair polymorphism. The value should be a space-separated list of &lt;/p&gt;
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&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
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&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;If one or more Targets is specified then a legal primer pair must flank at least one of them. A Target might be a simple sequence repeat site (for example a CA repeat) or a single-base-pair polymorphism. The value should be a space-separated list of &lt;/p&gt;
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&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;pairs where start is the index of the first base of a Target, and length is its length.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
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        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
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&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;A list of product size ranges, for example:&lt;/p&gt;
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&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;Primer3 first tries to pick primers in the first range. If that is not possible, it goes to the next range and tries again. It continues in this way until it has either picked all necessary primers or until there are no more ranges. For technical reasons this option makes much lighter computational demands than the Product Size option.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
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&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;A list of product size ranges, for example:&lt;/p&gt;
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&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;Primer3 first tries to pick primers in the first range. If that is not possible, it goes to the next range and tries again. It continues in this way until it has either picked all necessary primers or until there are no more ranges. For technical reasons this option makes much lighter computational demands than the Product Size option.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
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    <message>
        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
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&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;This selection indicates what mispriming library (if any) Primer3 should use to screen for&lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;interspersed repeats or for other sequence to avoid as a location for primers.&lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
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&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
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    <message>
        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
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&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;The maximum number of primer pairs to return. &lt;/span&gt;&lt;/p&gt;
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        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
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    <message>
        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
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&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;The maximum allowed weighted similarity with any sequence in Mispriming Library.&lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
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&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:8.25pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;The maximum allowed weighted similarity with any sequence in Mispriming Library.&lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
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    <message>
        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
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&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:8.25pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;The maximum allowed sum of similarities of a primer pair (one similarity for each primer) with any single sequence in Mispriming Library. &lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Library sequence weights are not used in computing the sum of similarities.&lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
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&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:8.25pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;The maximum allowed sum of similarities of a primer pair (one similarity for each primer) with any single sequence in Mispriming Library. &lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Library sequence weights are not used in computing the sum of similarities.&lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
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    <message>
        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:8.25pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;The maximum allowed similarity to ectopic sites in the sequence from which you are designing the primers. &lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot;-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-size:8pt;&quot;&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;The scoring system is the same as used for Max Mispriming, except that an ambiguity code is never treated as a consensus.&lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
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&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;The maximum allowed similarity to ectopic sites in the sequence from which you are designing the primers. &lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot;-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-size:8pt;&quot;&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;The scoring system is the same as used for Max Mispriming, except that an ambiguity code is never treated as a consensus.&lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
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    <message>
        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:8.25pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;The maximum allowed summed similarity of both primers to ectopic sites in the sequence from which you are designing the primers. &lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot;-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-size:8pt;&quot;&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;The scoring system is the same as used for Max Mispriming, except that an ambiguity code is never treated as a consensus.&lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:8.25pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;The maximum allowed summed similarity of both primers to ectopic sites in the sequence from which you are designing the primers. &lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot;-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-size:8pt;&quot;&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;The scoring system is the same as used for Max Mispriming, except that an ambiguity code is never treated as a consensus.&lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
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    <message>
        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:8.25pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Index of the first base of a start codon. &lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot;-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-size:8pt;&quot;&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;This parameter allows Primer3 to select primer pairs to create in-frame amplicons e.g. to create a template for a fusion protein. Primer3 will attempt to select an in-frame left primer, ideally starting at or to the left of the start codon, or to the right if necessary. Negative values of this parameter are legal if the actual start codon is to the left of available sequence. If this parameter is non-negative Primer3 signals an error if the codon at the position specified by this parameter is not an ATG. A value less than or equal to -10^6 indicates that Primer3 should ignore this parameter. Primer3 selects the position of the right primer by scanning right from the left primer for a stop codon. Ideally the right primer will end at or after the stop codon.&lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot;-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-size:8pt;&quot;&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;This parameter should be considered EXPERIMENTAL. Please check the output carefully; some erroneous inputs might cause an error in Primer3. &lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt; &lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:8.25pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Index of the first base of a start codon. &lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot;-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-size:8pt;&quot;&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;This parameter allows Primer3 to select primer pairs to create in-frame amplicons e.g. to create a template for a fusion protein. Primer3 will attempt to select an in-frame left primer, ideally starting at or to the left of the start codon, or to the right if necessary. Negative values of this parameter are legal if the actual start codon is to the left of available sequence. If this parameter is non-negative Primer3 signals an error if the codon at the position specified by this parameter is not an ATG. A value less than or equal to -10^6 indicates that Primer3 should ignore this parameter. Primer3 selects the position of the right primer by scanning right from the left primer for a stop codon. Ideally the right primer will end at or after the stop codon.&lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot;-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-size:8pt;&quot;&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;This parameter should be considered EXPERIMENTAL. Please check the output carefully; some erroneous inputs might cause an error in Primer3. &lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt; &lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
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        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
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    <message>
        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
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&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:8.25pt; font-weight:400; font-style:normal;&quot;&gt;
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        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
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&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:8.25pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot;-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-size:8pt;&quot;&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
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        <location filename="../src/Primer3Dialog.ui" line="568"/>
        <location filename="../src/Primer3Dialog.ui" line="2447"/>
        <source>Product Tm</source>
        <translation>Product Tm</translation>
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        <source>Primer GC%</source>
        <translation>Primer GC%</translation>
    </message>
    <message>
        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:8.25pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Minimum, Optimum, and Maximum lengths (in bases) of a primer oligo. &lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot;-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-size:8pt;&quot;&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Primer3 will not pick primers shorter than Min or longer than Max, and with default arguments will attempt to pick primers close with size close to Opt. &lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Min cannot be smaller than 1. Max cannot be larger than 36. (This limit is governed by maximum oligo size for which melting-temperature calculations are valid.) &lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Min cannot be greater than Max.&lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:8.25pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Minimum, Optimum, and Maximum lengths (in bases) of a primer oligo. &lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot;-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-size:8pt;&quot;&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Primer3 will not pick primers shorter than Min or longer than Max, and with default arguments will attempt to pick primers close with size close to Opt. &lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Min cannot be smaller than 1. Max cannot be larger than 36. (This limit is governed by maximum oligo size for which melting-temperature calculations are valid.) &lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Min cannot be greater than Max.&lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
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        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
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&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:8.25pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Minimum, Optimum, and Maximum melting temperatures (Celsius) for a primer oligo. &lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot;-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-size:8pt;&quot;&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Primer3 will not pick oligos with temperatures smaller than Min or larger than Max, and with&lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;default conditions will try to pick primers with melting temperatures close to Opt.&lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:8.25pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Minimum, Optimum, and Maximum melting temperatures (Celsius) for a primer oligo. &lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot;-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-size:8pt;&quot;&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Primer3 will not pick oligos with temperatures smaller than Min or larger than Max, and with&lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;default conditions will try to pick primers with melting temperatures close to Opt.&lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
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    <message>
        <source>Minimum, Optimum, and Maximum percentage of Gs and Cs in any primer or oligo.</source>
        <translation type="obsolete">Minimum, Optimum, and Maximum percentage of Gs and Cs in any primer or oligo.</translation>
    </message>
    <message>
        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:8.25pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;The minimum, optimum, and maximum melting temperature of the amplicon. &lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot;-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-size:8pt;&quot;&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Primer3 will not pick a product with melting temperature less than min or greater than max. If Opt is supplied and the Penalty Weights for Product Size are non-0 Primer3 will attempt to pick an amplicon with melting temperature close to Opt.&lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:8.25pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;The minimum, optimum, and maximum melting temperature of the amplicon. &lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot;-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-size:8pt;&quot;&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Primer3 will not pick a product with melting temperature less than min or greater than max. If Opt is supplied and the Penalty Weights for Product Size are non-0 Primer3 will attempt to pick an amplicon with melting temperature close to Opt.&lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <source>Maximum acceptable (unsigned) difference between the melting temperatures of the left and right primers.</source>
        <translation type="obsolete">Maximum acceptable (unsigned) difference between the melting temperatures of the left and right primers.</translation>
    </message>
    <message>
        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Specifies details of melting temperature calculation. &lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot;-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-size:8pt;&quot;&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;First method uses the table of thermodynamic parameters from the paper &lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;[Breslauer KJ, et. al (1986), Proc Natl Acad Sci 83:4746-50 http://dx.doi.org/10.1073/pnas.83.11.3746]&lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot;-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-size:8pt;&quot;&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Second method (recommended) was suggested in the paper [SantaLucia JR (1998)&lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;, Proc Natl Acad Sci 95:1460-65 http://dx.doi.org/10.1073/pnas.95.4.1460].&lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Specifies details of melting temperature calculation. &lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot;-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-size:8pt;&quot;&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;First method uses the table of thermodynamic parameters from the paper &lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;[Breslauer KJ, et. al (1986), Proc Natl Acad Sci 83:4746-50 http://dx.doi.org/10.1073/pnas.83.11.3746]&lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot;-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-size:8pt;&quot;&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Second method (recommended) was suggested in the paper [SantaLucia JR (1998)&lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;, Proc Natl Acad Sci 95:1460-65 http://dx.doi.org/10.1073/pnas.95.4.1460].&lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1015"/>
        <source>Max self complementarity</source>
        <translation>Max self complementarity</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1022"/>
        <source>Max 3&apos; self complementarity</source>
        <translation>Max 3&apos; self complementarity</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1029"/>
        <source>Inside target penalty</source>
        <translation>Inside target penalty</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1036"/>
        <source>Outside target penalty</source>
        <translation>Outside target penalty</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1043"/>
        <source>Concentration of monovalent cations</source>
        <translation>Concentration of monovalent cations</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1050"/>
        <source>Max #N&apos;s</source>
        <translation>Max #N&apos;s</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1057"/>
        <source>Max poly-X</source>
        <translation>Max poly-X</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1064"/>
        <source>First base index</source>
        <translation>First base index</translation>
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    <message>
        <location filename="../src/Primer3Dialog.ui" line="1071"/>
        <source>CG clamp</source>
        <translation>CG clamp</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1078"/>
        <source>Concentration of divalent cations</source>
        <translation>Concentration of divalent cations</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1085"/>
        <source>Salt correction formula</source>
        <translation>Salt correction formula</translation>
    </message>
    <message>
        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Specifies the salt correction formula for the melting temperature calculation.&lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot;-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-size:8pt;&quot;&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Specifies the salt correction formula for the melting temperature calculation.&lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot;-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-size:8pt;&quot;&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
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    <message>
        <location filename="../src/Primer3Dialog.ui" line="1104"/>
        <source>Schildkraut and Lifson 1965</source>
        <translation>Schildkraut and Lifson 1965</translation>
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    <message>
        <location filename="../src/Primer3Dialog.ui" line="1109"/>
        <source>Owczarzy et. 2004</source>
        <translation>Owczarzy et. 2004</translation>
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    <message>
        <location filename="../src/Primer3Dialog.ui" line="1117"/>
        <source>Concentration of dNTPs</source>
        <translation>Concentration of dNTPs</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1124"/>
        <source>Annealing oligo concentration</source>
        <translation>Annealing oligo concentration</translation>
    </message>
    <message>
        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:8.25pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;The maximum allowable 3&apos;-anchored global alignment score when testing a single primer for&lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;self-complementarity, and the maximum allowable 3&apos;-anchored global alignment score when&lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;testing for complementarity between left and right primers.&lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:8.25pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;The maximum allowable 3&apos;-anchored global alignment score when testing a single primer for&lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;self-complementarity, and the maximum allowable 3&apos;-anchored global alignment score when&lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;testing for complementarity between left and right primers.&lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
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    <message>
        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:8.25pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;The maximum allowable local alignment score when testing a single primer for (local) self-complementarity and the maximum allowable local alignment score when testing for complementarity between left and right primers. &lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot;-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-size:8pt;&quot;&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Local self-complementarity is taken to predict the tendency of primers to anneal to each other without necessarily causing self-priming in the PCR. The scoring system gives 1.00 for complementary bases, -0.25 for a match of any base (or N) with an N, -1.00 for a mismatch, and -2.00 for a gap. Only single-base-pair gaps are allowed.&lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:8.25pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;The maximum allowable local alignment score when testing a single primer for (local) self-complementarity and the maximum allowable local alignment score when testing for complementarity between left and right primers. &lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot;-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-size:8pt;&quot;&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Local self-complementarity is taken to predict the tendency of primers to anneal to each other without necessarily causing self-priming in the PCR. The scoring system gives 1.00 for complementary bases, -0.25 for a match of any base (or N) with an N, -1.00 for a mismatch, and -2.00 for a gap. Only single-base-pair gaps are allowed.&lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <source>Maximum number of unknown bases (N) allowable in any primer.</source>
        <translation type="obsolete">Maximum number of unknown bases (N) allowable in any primer.</translation>
    </message>
    <message>
        <source>The maximum allowable length of a mononucleotide repeat, for example AAAAAA.</source>
        <translation type="obsolete">The maximum allowable length of a mononucleotide repeat, for example AAAAAA.</translation>
    </message>
    <message>
        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p align=&quot;justify&quot; style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Non-default values valid only for sequences with 0 or 1 target regions. &lt;/span&gt;&lt;/p&gt;
&lt;p align=&quot;justify&quot; style=&quot;-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-size:8pt;&quot;&gt;&lt;/p&gt;
&lt;p align=&quot;justify&quot; style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;If the primer is part of a pair that spans a target and does not overlap the target, &lt;/span&gt;&lt;/p&gt;
&lt;p align=&quot;justify&quot; style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;then multiply this value times the number of nucleotide positions from the 3&apos; end &lt;/span&gt;&lt;/p&gt;
&lt;p align=&quot;justify&quot; style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;to the (unique) target to get the &apos;position penalty&apos;. The effect of this parameter is &lt;/span&gt;&lt;/p&gt;
&lt;p align=&quot;justify&quot; style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;to allow Primer3 to include nearness to the target as a term in the objective function.&lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p align=&quot;justify&quot; style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Non-default values valid only for sequences with 0 or 1 target regions. &lt;/span&gt;&lt;/p&gt;
&lt;p align=&quot;justify&quot; style=&quot;-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-size:8pt;&quot;&gt;&lt;/p&gt;
&lt;p align=&quot;justify&quot; style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;If the primer is part of a pair that spans a target and does not overlap the target, &lt;/span&gt;&lt;/p&gt;
&lt;p align=&quot;justify&quot; style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;then multiply this value times the number of nucleotide positions from the 3&apos; end &lt;/span&gt;&lt;/p&gt;
&lt;p align=&quot;justify&quot; style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;to the (unique) target to get the &apos;position penalty&apos;. The effect of this parameter is &lt;/span&gt;&lt;/p&gt;
&lt;p align=&quot;justify&quot; style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;to allow Primer3 to include nearness to the target as a term in the objective function.&lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;This parameter is the index of the first base in the input sequence.&lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot;-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-size:8pt;&quot;&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;This parameter is the index of the first base in the input sequence.&lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot;-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-size:8pt;&quot;&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Require the specified number of consecutive Gs and Cs at the 3&apos; end of both the left and right primer.&lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;This parameter has no effect on the internal oligo if one is requested.&lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Require the specified number of consecutive Gs and Cs at the 3&apos; end of both the left and right primer.&lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;This parameter has no effect on the internal oligo if one is requested.&lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;The millimolar (mM) concentration of monovalent salt cations (usually KCl) in the PCR. &lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Primer3 uses this argument to calculate oligo and primer melting temperatures. &lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;The millimolar (mM) concentration of monovalent salt cations (usually KCl) in the PCR. &lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Primer3 uses this argument to calculate oligo and primer melting temperatures. &lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;The millimolar concentration of divalent salt cations (usually MgCl^(2+)) in the PCR. &lt;/p&gt;
&lt;p style=&quot;-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;Primer3 converts concentration of divalent cations to concentration of monovalent cations using following formula:&lt;/p&gt;
&lt;p style=&quot;-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-weight:600;&quot;&gt;[Monovalent cations] = [Monovalent cations] + 120*(([divalent cations] - [dNTP])^0.5)&lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot;-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-weight:600;&quot;&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;In addition, if the specified concentration of dNTPs is larger than the concentration of divalent cations  then &lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;the effect of the divalent cations is not considered. &lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;The millimolar concentration of divalent salt cations (usually MgCl^(2+)) in the PCR. &lt;/p&gt;
&lt;p style=&quot;-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;Primer3 converts concentration of divalent cations to concentration of monovalent cations using following formula:&lt;/p&gt;
&lt;p style=&quot;-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-weight:600;&quot;&gt;[Monovalent cations] = [Monovalent cations] + 120*(([divalent cations] - [dNTP])^0.5)&lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot;-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-weight:600;&quot;&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;In addition, if the specified concentration of dNTPs is larger than the concentration of divalent cations  then &lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;the effect of the divalent cations is not considered. &lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;The millimolar concentration of the sum of all deoxyribonucleotide triphosphates. &lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot;-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-size:8pt;&quot;&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;The millimolar concentration of the sum of all deoxyribonucleotide triphosphates. &lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot;-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-size:8pt;&quot;&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;A value to use as nanomolar (nM) concentration of each annealing oligo over the course the PCR. &lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Primer3 uses this argument to esimate oligo melting temperatures.&lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot;-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-size:8pt;&quot;&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;The default (50nM) works well with the standard protocol used at the Whitehead/MIT Center for Genome Research --0.5 microliters of 20 micromolar concentration for each primer in a 20 microliter reaction with 10 nanograms template, 0.025 units/microliter Taq polymerase in 0.1 mM each dNTP, 1.5mM MgCl2, 50mM KCl, 10mM Tris-HCL (pH 9.3) using 35 cycles with an annealing temperature of 56 degrees Celsius.&lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot;-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-size:8pt;&quot;&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;The value of this parameter is less than the actual concentration of oligos in the initial reaction mix because  it is the concentration of annealing oligos, which in turn depends on the amount of template (including PCR product) in a given cycle. This concentration increases a great deal during a PCR; fortunately PCR seems quite robust for a variety&lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt; of oligo melting temperatures. &lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot;-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-size:8pt;&quot;&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;A value to use as nanomolar (nM) concentration of each annealing oligo over the course the PCR. &lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Primer3 uses this argument to esimate oligo melting temperatures.&lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot;-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-size:8pt;&quot;&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;The default (50nM) works well with the standard protocol used at the Whitehead/MIT Center for Genome Research --0.5 microliters of 20 micromolar concentration for each primer in a 20 microliter reaction with 10 nanograms template, 0.025 units/microliter Taq polymerase in 0.1 mM each dNTP, 1.5mM MgCl2, 50mM KCl, 10mM Tris-HCL (pH 9.3) using 35 cycles with an annealing temperature of 56 degrees Celsius.&lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot;-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-size:8pt;&quot;&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;The value of this parameter is less than the actual concentration of oligos in the initial reaction mix because  it is the concentration of annealing oligos, which in turn depends on the amount of template (including PCR product) in a given cycle. This concentration increases a great deal during a PCR; fortunately PCR seems quite robust for a variety&lt;/span&gt;&lt;/p&gt;
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&lt;p style=&quot;-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-size:8pt;&quot;&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Non-default values valid only for sequences with 0 or 1 target regions.&lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot;-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-size:8pt;&quot;&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;If the primer is part of a pair that spans a target and overlaps the target, then multiply this value times&lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt; the number of nucleotide positions by which the primer overlaps the (unique) target to get the &apos;position penalty&apos;. &lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;The effect of this parameter is to allow Primer3 to include overlap with the target as a term in the objective function.&lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Non-default values valid only for sequences with 0 or 1 target regions.&lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot;-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-size:8pt;&quot;&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;If the primer is part of a pair that spans a target and overlaps the target, then multiply this value times&lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt; the number of nucleotide positions by which the primer overlaps the (unique) target to get the &apos;position penalty&apos;. &lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;The effect of this parameter is to allow Primer3 to include overlap with the target as a term in the objective function.&lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
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        <source> Liberal base</source>
        <translation> Liberal base</translation>
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    <message>
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        <source>Show debuging info</source>
        <translation>Show debuging info</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1453"/>
        <source>Do not treat ambiguity codes in libraries as consensus</source>
        <translation>Do not treat ambiguity codes in libraries as consensus</translation>
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    <message>
        <location filename="../src/Primer3Dialog.ui" line="1463"/>
        <source>Lowercase masking</source>
        <translation>Lowercase masking</translation>
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    <message>
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        <source>Internal Oligo</source>
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    <message>
        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
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        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:8.25pt; font-weight:400; font-style:normal;&quot;&gt;
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    <message>
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        <source>Hyb oligo excluded region</source>
        <translation>Hyb oligo excluded region</translation>
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    <message>
        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
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&lt;p style=&quot;-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-size:8pt;&quot;&gt;&lt;/p&gt;
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&lt;p style=&quot;-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-size:8pt; font-weight:600;&quot;&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;pairs, where &amp;lt;start&amp;gt; is the index of the first base of an excluded region, and &amp;lt;length&amp;gt; is its length. Often one would make Target regions excluded regions for internal oligos.&lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Middle oligos may not overlap any region specified by this tag. The associated value must be a space-separated list of&lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot;-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-size:8pt;&quot;&gt;&lt;/p&gt;
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        <source>Hyb oligo size</source>
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        <location filename="../src/Primer3Dialog.ui" line="2759"/>
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        <source>Hyb oligo GC%</source>
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        <location filename="../src/Primer3Dialog.ui" line="1537"/>
        <location filename="../src/Primer3Dialog.ui" line="1544"/>
        <source>Min:</source>
        <translation>Min:</translation>
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    <message>
        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
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        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
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    <message>
        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
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        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
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&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
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    </message>
    <message>
        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
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        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Equivalent parameter of primer GC% for the internal oligo. &lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
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    <message>
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        <location filename="../src/Primer3Dialog.ui" line="1606"/>
        <location filename="../src/Primer3Dialog.ui" line="1613"/>
        <source>Opt:</source>
        <translation>Opt:</translation>
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    <message>
        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Equivalent parameter of Primer GC% for the internal oligo. &lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Equivalent parameter of Primer GC% for the internal oligo. &lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1659"/>
        <location filename="../src/Primer3Dialog.ui" line="1666"/>
        <location filename="../src/Primer3Dialog.ui" line="1673"/>
        <source>Max:</source>
        <translation>Max:</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1730"/>
        <location filename="../src/Primer3Dialog.ui" line="2929"/>
        <source>Hyb oligo self complementarity</source>
        <translation>Hyb oligo self complementarity</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1737"/>
        <source>Max #Ns</source>
        <translation>Max #Ns</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1744"/>
        <source>Hyb oligo mishyb library</source>
        <translation>Hyb oligo mishyb library</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1751"/>
        <source>Hyb oligo min sequence quality</source>
        <translation>Hyb oligo min sequence quality</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1758"/>
        <source>Hyb oligo conc of monovalent cations</source>
        <translation>Hyb oligo conc of monovalent cations</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1765"/>
        <source>Hyb oligo conc of divalent cations</source>
        <translation>Hyb oligo conc of divalent cations</translation>
    </message>
    <message>
        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Equivalent parameter of primer max self complemntarity for the internal oligo. &lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Equivalent parameter of primer max self complemntarity for the internal oligo. &lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Equivalent parameter of primer max #Ns for the internal oligo. &lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Equivalent parameter of primer max #Ns for the internal oligo. &lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Similar to primer mispriming library, except that the event we seek to avoid is hybridization of the internal oligo to sequences in this library rather than priming from them.&lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Similar to primer mispriming library, except that the event we seek to avoid is hybridization of the internal oligo to sequences in this library rather than priming from them.&lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Equivalent parameter of primer minimum quality for the internal oligo. &lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Equivalent parameter of primer minimum quality for the internal oligo. &lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Equivalent parameter of primer concentration of monovalent cations for the internal oligo. &lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Equivalent parameter of primer concentration of monovalent cations for the internal oligo. &lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Equivalent parameter of primer concentration of divalent cations for the internal oligo. &lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Equivalent parameter of primer concentration of divalent cations for the internal oligo. &lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1865"/>
        <source>Hyb oligo max 3&apos; self complementarity</source>
        <translation>Hyb oligo max 3&apos; self complementarity</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1872"/>
        <source>Hyb oligo max poly-X</source>
        <translation>Hyb oligo max poly-X</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1879"/>
        <source>Hyb oligo max mishyb</source>
        <translation>Hyb oligo max mishyb</translation>
    </message>
    <message>
        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Equivalent parameter of primer max 3&apos; self complementarity for the internal oligo. &lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Equivalent parameter of primer max 3&apos; self complementarity for the internal oligo. &lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Equivalent parameter of primer max poly-X for the internal oligo. &lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Equivalent parameter of primer max poly-X for the internal oligo. &lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Similar to primer max library mispriming. &lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Similar to primer max library mispriming. &lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1934"/>
        <source>Hyb oligo [dNTP]</source>
        <translation>Hyb oligo [dNTP]</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1941"/>
        <source>Hyb oligo DNA concentration</source>
        <translation>Hyb oligo DNA concentration</translation>
    </message>
    <message>
        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Equivalent parameter of primer concentration of the dNTPs for the internal oligo. &lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Гибридизация олиго [dNTP]. &lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Equivalent parameter of primer DNA concentration for the internal oligo. &lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
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&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
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&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;ДНК концентрация гибридизации олиго. &lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
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        <source>Penalty Weights</source>
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&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
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&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:8.25pt; font-weight:400; font-style:normal;&quot;&gt;
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&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
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&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;There are two classes of weights: for some parameters there is a &apos;Lt&apos; (less than) and a &apos;Gt&apos; (greater than) weight. These are the weights that Primer3 uses when the value is less or greater than (respectively) the specified optimum.&lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
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&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;Sans Serif&apos;; font-size:10pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:14pt; font-weight:600;&quot;&gt;For Primers&lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
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        <source>Tm</source>
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        <source>Lt:</source>
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&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
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        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
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        <location filename="../src/Primer3Dialog.ui" line="2119"/>
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&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
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&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Penalty weight for a primer parameter different from predefined optimum value. &lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
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&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
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        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
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    <message>
        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
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        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
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&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;Sans Serif&apos;; font-size:10pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:14pt; font-weight:600;&quot;&gt;For Hyb Oligos&lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation type="vanished">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;Sans Serif&apos;; font-size:10pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:14pt; font-weight:600;&quot;&gt;For Hyb Oligos&lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Penalty weight for corressponding parameter of a internal oligo less than (Lt) or greater than (Gt) the optimal value. &lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Penalty weight for corressponding parameter of a internal oligo less than (Lt) or greater than (Gt) the optimal value. &lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
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        <source>Hyb oligo #N&apos;s</source>
        <translation>Hyb oligo #N&apos;s</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="2943"/>
        <source>Hyb oligo mishybing</source>
        <translation>Hyb oligo mishybing</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="2950"/>
        <source>Hyb oligo sequence quality</source>
        <translation>Hyb oligo sequence quality</translation>
    </message>
    <message>
        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Penalty weight for an internal oligo parameter different from predefined optimum value.&lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;Penalty weight for an internal oligo parameter different from predefined optimum value.&lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
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    <message>
        <location filename="../src/Primer3Dialog.ui" line="3039"/>
        <source>RT-PCR</source>
        <translation>RT-PCR</translation>
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    <message>
        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;Sans Serif&apos;; font-size:9pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:8pt;&quot;&gt;In this section user can specify if primer product must overlap exon-exon junction or span intron. This only applies when designing primers for a cDNA (mRNA) sequence with annotated exons. &lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;Sans Serif&apos;; font-size:9pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:8pt;&quot;&gt;RT-PCR. &lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;Sans Serif&apos;; font-size:9pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:8pt;&quot;&gt;This feature allows to specify if primer product must span intron-exon boundaries. &lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:8pt;&quot;&gt;Checking this option will result in ignoring &lt;/span&gt;&lt;span style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:8pt; font-style:italic;&quot;&gt;Excluded&lt;/span&gt;&lt;span style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:8pt;&quot;&gt; and &lt;/span&gt;&lt;span style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:8pt; font-style:italic;&quot;&gt;Target Regions&lt;/span&gt;&lt;span style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:8pt;&quot;&gt; from Main section.&lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;Sans Serif&apos;; font-size:9pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:8pt;&quot;&gt;This feature allows to specify if primer product must span intron-exon boundaries. &lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:8pt;&quot;&gt;Checking this option will result in ignoring &lt;/span&gt;&lt;span style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:8pt; font-style:italic;&quot;&gt;Excluded&lt;/span&gt;&lt;span style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:8pt;&quot;&gt; and &lt;/span&gt;&lt;span style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:8pt; font-style:italic;&quot;&gt;Target Regions&lt;/span&gt;&lt;span style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:8pt;&quot;&gt; from Main section.&lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
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        <location filename="../src/Primer3Dialog.ui" line="3051"/>
        <source>Design primers for RT-PCR analysis</source>
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    <message>
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        <source>Exon annotation name:</source>
        <translation>Exon annotation name:</translation>
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    <message>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;The name of the annotation which defines the exons in the mRNA sequence.&lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation type="obsolete">&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;The name of the annotation which defines the exons in the mRNA sequence.&lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
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    <message>
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        <source>exon</source>
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    <message>
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        <source>Minimum exon junction overlap size</source>
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    <message>
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        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;If the range is set, primer search will be restricted to selected exons. For example:&lt;/p&gt;&lt;p&gt;&lt;span style=&quot; font-weight:600;&quot;&gt;1-5&lt;/span&gt;&lt;/p&gt;&lt;p&gt;If the range is larger than actual exon range or the starting exon number exceeds number of exons, error message is shown. &lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;If the range is set, primer search will be restricted to selected exons. For example:&lt;/p&gt;&lt;p&gt;&lt;span style=&quot; font-weight:600;&quot;&gt;1-5&lt;/span&gt;&lt;/p&gt;&lt;p&gt;If the range is larger than actual exon range or the starting exon number exceeds number of exons, error message is shown. &lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
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    <message>
        <location filename="../src/Primer3Dialog.ui" line="3224"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;This number of found primer pairs wil bel queried to check if they fullfill the requirements for RTPCR.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;This number of found primer pairs wil bel queried to check if they fullfill the requirements for RTPCR.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="3282"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;In this section user can specify sequence quality of target sequence and related parameters.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;In this section user can specify sequence quality of target sequence and related parameters.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="3295"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;A list of space separated integers. There must be exactly one integer for each base in the Source Sequence if this argument is non-empty. High numbers indicate high confidence in the base call at that position and low numbers indicate low confidence in the base call at that position.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;A list of space separated integers. There must be exactly one integer for each base in the Source Sequence if this argument is non-empty. High numbers indicate high confidence in the base call at that position and low numbers indicate low confidence in the base call at that position.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="3317"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;The minimum sequence quality allowed within a primer.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;The minimum sequence quality allowed within a primer.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="3340"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;The minimum sequence quality allowed within the 3&apos; pentamer of a primer.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;The minimum sequence quality allowed within the 3&apos; pentamer of a primer.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="3363"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;The minimum legal sequence quality.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;The minimum legal sequence quality.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="3386"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;The maximum legal sequence quality.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;The maximum legal sequence quality.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="3402"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;This section allows to set output annotations&apos; settings.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;This section allows to set output annotations&apos; settings.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="3457"/>
        <source>Help</source>
        <translation>Help</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="3477"/>
        <source>Save settings</source>
        <translation>Save settings</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="3484"/>
        <source>Load settings</source>
        <translation>Load settings</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="3491"/>
        <source>Reset form</source>
        <translation>Reset form</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="3498"/>
        <source>Pick primers</source>
        <translation>Pick primers</translation>
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    <message>
        <location filename="../src/Primer3Dialog.ui" line="3063"/>
        <source>mRNA sequence</source>
        <translation>mRNA sequence</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1095"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Specifies the salt correction formula for the melting temperature calculation.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Specifies the salt correction formula for the melting temperature calculation.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1254"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;The maximum allowable 3&apos;-anchored global alignment score when testing a single primer for self-complementarity, and the maximum allowable 3&apos;-anchored global alignment score when testing for complementarity between left and right primers.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;The maximum allowable 3&apos;-anchored global alignment score when testing a single primer for self-complementarity, and the maximum allowable 3&apos;-anchored global alignment score when testing for complementarity between left and right primers.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1270"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;The maximum allowable local alignment score when testing a single primer for (local) self-complementarity and the maximum allowable local alignment score when testing for complementarity between left and right primers.&lt;/p&gt;&lt;p&gt;Local self-complementarity is taken to predict the tendency of primers to anneal to each other without necessarily causing self-priming in the PCR. The scoring system gives 1.00 for complementary bases, -0.25 for a match of any base (or N) with an N, -1.00 for a mismatch, and -2.00 for a gap. Only single-base-pair gaps are allowed.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;The maximum allowable local alignment score when testing a single primer for (local) self-complementarity and the maximum allowable local alignment score when testing for complementarity between left and right primers.&lt;/p&gt;&lt;p&gt;Local self-complementarity is taken to predict the tendency of primers to anneal to each other without necessarily causing self-priming in the PCR. The scoring system gives 1.00 for complementary bases, -0.25 for a match of any base (or N) with an N, -1.00 for a mismatch, and -2.00 for a gap. Only single-base-pair gaps are allowed.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1286"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Maximum number of unknown bases (N) allowable in any primer.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Maximum number of unknown bases (N) allowable in any primer.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1302"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;The maximum allowable length of a mononucleotide repeat, for example AAAAAA.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;The maximum allowable length of a mononucleotide repeat, for example AAAAAA.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1318"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Non-default values valid only for sequences with 0 or 1 target regions.&lt;/p&gt;&lt;p&gt;If the primer is part of a pair that spans a target and does not overlap the target, then multiply this value times the number of nucleotide positions from the 3&apos; end to the (unique) target to get the &apos;position penalty&apos;. The effect of this parameter is to allow Primer3 to include nearness to the target as a term in the objective function.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Non-default values valid only for sequences with 0 or 1 target regions.&lt;/p&gt;&lt;p&gt;If the primer is part of a pair that spans a target and does not overlap the target, then multiply this value times the number of nucleotide positions from the 3&apos; end to the (unique) target to get the &apos;position penalty&apos;. The effect of this parameter is to allow Primer3 to include nearness to the target as a term in the objective function.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1334"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;This parameter is the index of the first base in the input sequence.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;This parameter is the index of the first base in the input sequence.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1350"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Require the specified number of consecutive Gs and Cs at the 3&apos; end of both the left and right primer.&lt;/p&gt;&lt;p&gt;This parameter has no effect on the internal oligo if one is requested.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Require the specified number of consecutive Gs and Cs at the 3&apos; end of both the left and right primer.&lt;/p&gt;&lt;p&gt;This parameter has no effect on the internal oligo if one is requested.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1366"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;The millimolar (mM) concentration of monovalent salt cations (usually KCl) in the PCR.&lt;/p&gt;&lt;p&gt;Primer3 uses this argument to calculate oligo and primer melting temperatures.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;The millimolar (mM) concentration of monovalent salt cations (usually KCl) in the PCR.&lt;/p&gt;&lt;p&gt;Primer3 uses this argument to calculate oligo and primer melting temperatures.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1382"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;The millimolar concentration of divalent salt cations (usually MgCl^(2+)) in the PCR.&lt;/p&gt;&lt;p&gt;Primer3 converts concentration of divalent cations to concentration of monovalent cations using following formula:&lt;/p&gt;&lt;p&gt;&lt;span style=&quot; font-weight:600;&quot;&gt;[Monovalent cations] = [Monovalent cations] + 120*(([divalent cations] - [dNTP])^0.5)&lt;/span&gt;&lt;/p&gt;&lt;p&gt;In addition, if the specified concentration of dNTPs is larger than the concentration of divalent cations then the effect of the divalent cations is not considered.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;The millimolar concentration of divalent salt cations (usually MgCl^(2+)) in the PCR.&lt;/p&gt;&lt;p&gt;Primer3 converts concentration of divalent cations to concentration of monovalent cations using following formula:&lt;/p&gt;&lt;p&gt;&lt;span style=&quot; font-weight:600;&quot;&gt;[Monovalent cations] = [Monovalent cations] + 120*(([divalent cations] - [dNTP])^0.5)&lt;/span&gt;&lt;/p&gt;&lt;p&gt;In addition, if the specified concentration of dNTPs is larger than the concentration of divalent cations then the effect of the divalent cations is not considered.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1395"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;The millimolar concentration of the sum of all deoxyribonucleotide triphosphates.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;The millimolar concentration of the sum of all deoxyribonucleotide triphosphates.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1408"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;A value to use as nanomolar (nM) concentration of each annealing oligo over the course the PCR.&lt;/p&gt;&lt;p&gt;Primer3 uses this argument to esimate oligo melting temperatures.&lt;/p&gt;&lt;p&gt;The default (50nM) works well with the standard protocol used at the Whitehead/MIT Center for Genome Research --0.5 microliters of 20 micromolar concentration for each primer in a 20 microliter reaction with 10 nanograms template, 0.025 units/microliter Taq polymerase in 0.1 mM each dNTP, 1.5mM MgCl2, 50mM KCl, 10mM Tris-HCL (pH 9.3) using 35 cycles with an annealing temperature of 56 degrees Celsius.&lt;/p&gt;&lt;p&gt;The value of this parameter is less than the actual concentration of oligos in the initial reaction mix because  it is the concentration of annealing oligos, which in turn depends on the amount of template (including PCR product) in a given cycle. This concentration increases a great deal during a PCR; fortunately PCR seems quite robust for a variety of oligo melting temperatures.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;A value to use as nanomolar (nM) concentration of each annealing oligo over the course the PCR.&lt;/p&gt;&lt;p&gt;Primer3 uses this argument to esimate oligo melting temperatures.&lt;/p&gt;&lt;p&gt;The default (50nM) works well with the standard protocol used at the Whitehead/MIT Center for Genome Research --0.5 microliters of 20 micromolar concentration for each primer in a 20 microliter reaction with 10 nanograms template, 0.025 units/microliter Taq polymerase in 0.1 mM each dNTP, 1.5mM MgCl2, 50mM KCl, 10mM Tris-HCL (pH 9.3) using 35 cycles with an annealing temperature of 56 degrees Celsius.&lt;/p&gt;&lt;p&gt;The value of this parameter is less than the actual concentration of oligos in the initial reaction mix because  it is the concentration of annealing oligos, which in turn depends on the amount of template (including PCR product) in a given cycle. This concentration increases a great deal during a PCR; fortunately PCR seems quite robust for a variety of oligo melting temperatures.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1415"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Non-default values valid only for sequences with 0 or 1 target regions.&lt;/p&gt;&lt;p&gt;If the primer is part of a pair that spans a target and overlaps the target, then multiply this value times the number of nucleotide positions by which the primer overlaps the (unique) target to get the &apos;position penalty&apos;.&lt;/p&gt;&lt;p&gt;The effect of this parameter is to allow Primer3 to include overlap with the target as a term in the objective function.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Non-default values valid only for sequences with 0 or 1 target regions.&lt;/p&gt;&lt;p&gt;If the primer is part of a pair that spans a target and overlaps the target, then multiply this value times the number of nucleotide positions by which the primer overlaps the (unique) target to get the &apos;position penalty&apos;.&lt;/p&gt;&lt;p&gt;The effect of this parameter is to allow Primer3 to include overlap with the target as a term in the objective function.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1476"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Parameters governing choice of internal oligos are analogous to the parameters governing choice of primer pairs.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Parameters governing choice of internal oligos are analogous to the parameters governing choice of primer pairs.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1491"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Middle oligos may not overlap any region specified by this tag. The associated value must be a space-separated list of&lt;/p&gt;&lt;p&gt;&lt;span style=&quot; font-weight:600;&quot;&gt;&amp;lt;start&amp;gt;,&amp;lt;length&amp;gt;&lt;/span&gt;&lt;/p&gt;&lt;p&gt;pairs, where &amp;lt;start&amp;gt; is the index of the first base of an excluded region, and &amp;lt;length&amp;gt; is its length. Often one would make Target regions excluded regions for internal oligos.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Middle oligos may not overlap any region specified by this tag. The associated value must be a space-separated list of&lt;/p&gt;&lt;p&gt;&lt;span style=&quot; font-weight:600;&quot;&gt;&amp;lt;start&amp;gt;,&amp;lt;length&amp;gt;&lt;/span&gt;&lt;/p&gt;&lt;p&gt;pairs, where &amp;lt;start&amp;gt; is the index of the first base of an excluded region, and &amp;lt;length&amp;gt; is its length. Often one would make Target regions excluded regions for internal oligos.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1557"/>
        <location filename="../src/Primer3Dialog.ui" line="1626"/>
        <location filename="../src/Primer3Dialog.ui" line="1686"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Equivalent parameter of Primer Size for the internal oligo.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Equivalent parameter of Primer Size for the internal oligo.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1573"/>
        <location filename="../src/Primer3Dialog.ui" line="1642"/>
        <location filename="../src/Primer3Dialog.ui" line="1699"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Equivalent parameter of Primer Tm for the internal oligo.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Equivalent parameter of Primer Tm for the internal oligo.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1589"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Equivalent parameter of primer GC% for the internal oligo.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Equivalent parameter of primer GC% for the internal oligo.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1652"/>
        <location filename="../src/Primer3Dialog.ui" line="1712"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Equivalent parameter of Primer GC% for the internal oligo.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Equivalent parameter of Primer GC% for the internal oligo.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1778"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Equivalent parameter of primer max self complemntarity for the internal oligo.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Equivalent parameter of primer max self complemntarity for the internal oligo.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1794"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Equivalent parameter of primer max #Ns for the internal oligo.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Equivalent parameter of primer max #Ns for the internal oligo.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1810"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Similar to primer mispriming library, except that the event we seek to avoid is hybridization of the internal oligo to sequences in this library rather than priming from them.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Similar to primer mispriming library, except that the event we seek to avoid is hybridization of the internal oligo to sequences in this library rather than priming from them.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1823"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Equivalent parameter of primer minimum quality for the internal oligo.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Equivalent parameter of primer minimum quality for the internal oligo.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1839"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Equivalent parameter of primer concentration of monovalent cations for the internal oligo.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Equivalent parameter of primer concentration of monovalent cations for the internal oligo.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1855"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Equivalent parameter of primer concentration of divalent cations for the internal oligo.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Equivalent parameter of primer concentration of divalent cations for the internal oligo.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1892"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Equivalent parameter of primer max 3&apos; self complementarity for the internal oligo.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Equivalent parameter of primer max 3&apos; self complementarity for the internal oligo.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1908"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Equivalent parameter of primer max poly-X for the internal oligo.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Equivalent parameter of primer max poly-X for the internal oligo.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1924"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Similar to primer max library mispriming.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Similar to primer max library mispriming.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1954"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Equivalent parameter of primer concentration of the dNTPs for the internal oligo.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Equivalent parameter of primer concentration of the dNTPs for the internal oligo.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1970"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Equivalent parameter of primer DNA concentration for the internal oligo.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Equivalent parameter of primer DNA concentration for the internal oligo.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="1999"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;This section describes &amp;quot;penalty weights&amp;quot;, which allow the user to modify the criteria that Primer3 uses to select the &amp;quot;best&amp;quot; primers.&lt;/p&gt;&lt;p&gt;There are two classes of weights: for some parameters there is a &apos;Lt&apos; (less than) and a &apos;Gt&apos; (greater than) weight. These are the weights that Primer3 uses when the value is less or greater than (respectively) the specified optimum.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;This section describes &amp;quot;penalty weights&amp;quot;, which allow the user to modify the criteria that Primer3 uses to select the &amp;quot;best&amp;quot; primers.&lt;/p&gt;&lt;p&gt;There are two classes of weights: for some parameters there is a &apos;Lt&apos; (less than) and a &apos;Gt&apos; (greater than) weight. These are the weights that Primer3 uses when the value is less or greater than (respectively) the specified optimum.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="2013"/>
        <source>Primers</source>
        <translation type="unfinished"></translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="2070"/>
        <location filename="../src/Primer3Dialog.ui" line="2086"/>
        <location filename="../src/Primer3Dialog.ui" line="2102"/>
        <location filename="../src/Primer3Dialog.ui" line="2139"/>
        <location filename="../src/Primer3Dialog.ui" line="2155"/>
        <location filename="../src/Primer3Dialog.ui" line="2171"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Penalty weight for corressponding parameter of a primer less than (Lt) or greater than (Gt) the optimal value.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Penalty weight for corressponding parameter of a primer less than (Lt) or greater than (Gt) the optimal value.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="2261"/>
        <location filename="../src/Primer3Dialog.ui" line="2277"/>
        <location filename="../src/Primer3Dialog.ui" line="2293"/>
        <location filename="../src/Primer3Dialog.ui" line="2309"/>
        <location filename="../src/Primer3Dialog.ui" line="2325"/>
        <location filename="../src/Primer3Dialog.ui" line="2341"/>
        <location filename="../src/Primer3Dialog.ui" line="2373"/>
        <location filename="../src/Primer3Dialog.ui" line="2389"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Penalty weight for a primer parameter different from predefined optimum value.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Penalty weight for a primer parameter different from predefined optimum value.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="2357"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Determines the overall weight of the position penalty in calculating the penalty for a primer.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Determines the overall weight of the position penalty in calculating the penalty for a primer.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="2431"/>
        <source>Primer Pairs</source>
        <translation type="unfinished"></translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="2460"/>
        <location filename="../src/Primer3Dialog.ui" line="2476"/>
        <location filename="../src/Primer3Dialog.ui" line="2520"/>
        <location filename="../src/Primer3Dialog.ui" line="2536"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Penalty weight for corressponding parameter of a primer pair less than (Lt) or greater than (Gt) the optimal value.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Penalty weight for corressponding parameter of a primer pair less than (Lt) or greater than (Gt) the optimal value.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="2612"/>
        <location filename="../src/Primer3Dialog.ui" line="2628"/>
        <location filename="../src/Primer3Dialog.ui" line="2644"/>
        <location filename="../src/Primer3Dialog.ui" line="2660"/>
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        <location filename="../src/Primer3Dialog.ui" line="2692"/>
        <location filename="../src/Primer3Dialog.ui" line="2708"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Penalty weight for a primer pair parameter different from predefined optimum value.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Penalty weight for a primer pair parameter different from predefined optimum value.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
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        <source>Hyb Oligos</source>
        <translation type="unfinished"></translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="2807"/>
        <location filename="../src/Primer3Dialog.ui" line="2823"/>
        <location filename="../src/Primer3Dialog.ui" line="2839"/>
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        <location filename="../src/Primer3Dialog.ui" line="2892"/>
        <location filename="../src/Primer3Dialog.ui" line="2908"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Penalty weight for corressponding parameter of a internal oligo less than (Lt) or greater than (Gt) the optimal value.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Penalty weight for corressponding parameter of a internal oligo less than (Lt) or greater than (Gt) the optimal value.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
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        <location filename="../src/Primer3Dialog.ui" line="2979"/>
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        <location filename="../src/Primer3Dialog.ui" line="3011"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Penalty weight for an internal oligo parameter different from predefined optimum value.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;Penalty weight for an internal oligo parameter different from predefined optimum value.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="3042"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;In this section user can specify if primer product must overlap exon-exon junction or span intron. This only applies when designing primers for a cDNA (mRNA) sequence with annotated exons.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;In this section user can specify if primer product must overlap exon-exon junction or span intron. This only applies when designing primers for a cDNA (mRNA) sequence with annotated exons.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="3048"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;This feature allows to specify if primer product must span intron-exon boundaries.&lt;/p&gt;&lt;p&gt;Checking this option will result in ignoring &lt;span style=&quot; font-style:italic;&quot;&gt;Excluded&lt;/span&gt; and &lt;span style=&quot; font-style:italic;&quot;&gt;Target Regions&lt;/span&gt; from Main section.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;This feature allows to specify if primer product must span intron-exon boundaries.&lt;/p&gt;&lt;p&gt;Checking this option will result in ignoring &lt;span style=&quot; font-style:italic;&quot;&gt;Excluded&lt;/span&gt; and &lt;span style=&quot; font-style:italic;&quot;&gt;Target Regions&lt;/span&gt; from Main section.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="3078"/>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;The name of the annotation which defines the exons in the mRNA sequence.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;The name of the annotation which defines the exons in the mRNA sequence.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="3118"/>
        <source>At 5&apos; side (bp):</source>
        <translation>At 5&apos; side (bp):</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="3148"/>
        <source>At 3&apos;side (bp)</source>
        <translation>At 3&apos;side (bp)</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="3170"/>
        <source>Exon range:</source>
        <translation>Exon range:</translation>
    </message>
    <message>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;If the range is set, primer search will be restricted to selected exons. For example:&lt;/span&gt;&lt;/p&gt;&lt;p&gt;&lt;span style=&quot; font-size:8pt; font-weight:600;&quot;&gt;1-5&lt;/span&gt;&lt;/p&gt;&lt;p&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;If the range is larger than actual exon range or the starting exon number exceeds number of exons, error message is shown. &lt;/span&gt;&lt;/p&gt;&lt;p&gt;&lt;br/&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation type="obsolete">&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;If the range is set, primer search will be restricted to selected exons. For example:&lt;/span&gt;&lt;/p&gt;&lt;p&gt;&lt;span style=&quot; font-size:8pt; font-weight:600;&quot;&gt;1-5&lt;/span&gt;&lt;/p&gt;&lt;p&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;If the range is larger than actual exon range or the starting exon number exceeds number of exons, error message is shown. &lt;/span&gt;&lt;/p&gt;&lt;p&gt;&lt;br/&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="3205"/>
        <source>Primer product must span at least one intron on the corresponding genomic DNA</source>
        <translation>Primer product must span at least one intron on the corresponding genomic DNA</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="3217"/>
        <source>Max number of pairs to query:</source>
        <translation>Max number of pairs to query:</translation>
    </message>
    <message>
        <source>&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;This number of found primer pairs wil bel queried to check if they fullfill the requirements for RTPCR&lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation type="obsolete">&lt;html&gt;&lt;head/&gt;&lt;body&gt;&lt;p&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;This number of found primer pairs wil bel queried to check if they fullfill the requirements for RTPCR&lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="3279"/>
        <source>Sequence Quality</source>
        <translation>Sequence Quality</translation>
    </message>
    <message>
        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;In this section user can specify sequence quality of target sequence and related parameters.&lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;In this section user can specify sequence quality of target sequence and related parameters.&lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:8.25pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p align=&quot;justify&quot; style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;A list of space separated integers. There must be exactly one integer for each base in the Source Sequence if this argument is non-empty. High numbers indicate high confidence in the base call at that position and low numbers indicate low confidence in the base call at that position.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:8.25pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p align=&quot;justify&quot; style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;A list of space separated integers. There must be exactly one integer for each base in the Source Sequence if this argument is non-empty. High numbers indicate high confidence in the base call at that position and low numbers indicate low confidence in the base call at that position.&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="3304"/>
        <source>Min sequence quality:</source>
        <translation>Min sequence quality:</translation>
    </message>
    <message>
        <source>The minimum sequence quality allowed within a primer.</source>
        <translation type="obsolete">The minimum sequence quality allowed within a primer.</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="3327"/>
        <source>Min 3&apos; sequence quality:</source>
        <translation>Min 3&apos; sequence quality:</translation>
    </message>
    <message>
        <source>The minimum sequence quality allowed within the 3&apos; pentamer of a primer.</source>
        <translation type="obsolete">The minimum sequence quality allowed within the 3&apos; pentamer of a primer.</translation>
    </message>
    <message>
        <source>&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;This section allows to set output annotations&apos; settings.&lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</source>
        <translation type="obsolete">&lt;!DOCTYPE HTML PUBLIC &quot;-//W3C//DTD HTML 4.0//EN&quot; &quot;http://www.w3.org/TR/REC-html40/strict.dtd&quot;&gt;
&lt;html&gt;&lt;head&gt;&lt;meta name=&quot;qrichtext&quot; content=&quot;1&quot; /&gt;&lt;style type=&quot;text/css&quot;&gt;
p, li { white-space: pre-wrap; }
&lt;/style&gt;&lt;/head&gt;&lt;body style=&quot; font-family:&apos;MS Shell Dlg 2&apos;; font-size:7.8pt; font-weight:400; font-style:normal;&quot;&gt;
&lt;p style=&quot; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;&quot;&gt;&lt;span style=&quot; font-size:8pt;&quot;&gt;This section allows to set output annotations&apos; settings.&lt;/span&gt;&lt;/p&gt;&lt;/body&gt;&lt;/html&gt;</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="3350"/>
        <source>Sequence quality range min:</source>
        <translation>Sequence quality range min:</translation>
    </message>
    <message>
        <source>The minimum legal sequence quality.</source>
        <translation type="obsolete">The minimum legal sequence quality.</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="3373"/>
        <source>Sequence quality range max:</source>
        <translation>Sequence quality range max:</translation>
    </message>
    <message>
        <source>The maximum legal sequence quality.</source>
        <translation type="obsolete">The maximum legal sequence quality.</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.ui" line="3399"/>
        <source>Result Settings</source>
        <translation>Result Settings</translation>
    </message>
</context>
<context>
    <name>QObject</name>
    <message>
        <location filename="../src/Primer3Query.cpp" line="172"/>
        <source>Primer</source>
        <translation>Primer</translation>
    </message>
    <message>
        <location filename="../src/Primer3Query.cpp" line="173"/>
        <source>PCR primer design</source>
        <translation>PCR primer design</translation>
    </message>
    <message>
        <location filename="../src/Primer3Query.cpp" line="175"/>
        <source>Excluded regions</source>
        <translation>Excluded regions</translation>
    </message>
    <message>
        <location filename="../src/Primer3Query.cpp" line="176"/>
        <source>Targets</source>
        <translation>Targets</translation>
    </message>
    <message>
        <location filename="../src/Primer3Query.cpp" line="177"/>
        <source>Product size ranges</source>
        <translation>Product size ranges</translation>
    </message>
    <message>
        <location filename="../src/Primer3Query.cpp" line="178"/>
        <source>Number to return</source>
        <translation>Number to return</translation>
    </message>
    <message>
        <location filename="../src/Primer3Query.cpp" line="180"/>
        <source>Max repeat mispriming</source>
        <translation>Max repeat mispriming</translation>
    </message>
    <message>
        <location filename="../src/Primer3Query.cpp" line="181"/>
        <source>Max template mispriming</source>
        <translation>Max template mispriming</translation>
    </message>
    <message>
        <location filename="../src/Primer3Query.cpp" line="182"/>
        <source>Max 3&apos; stability</source>
        <translation>Max 3&apos; stability</translation>
    </message>
    <message>
        <location filename="../src/Primer3Query.cpp" line="183"/>
        <source>Pair max repeat mispriming</source>
        <translation>Pair max repeat mispriming</translation>
    </message>
    <message>
        <location filename="../src/Primer3Query.cpp" line="184"/>
        <source>Pair max template mispriming</source>
        <translation>Pair max template mispriming</translation>
    </message>
</context>
<context>
    <name>U2::FindExonRegionsTask</name>
    <message>
        <location filename="../src/FindExonRegionsTask.cpp" line="98"/>
        <source>Failed to search for exon annotations. The sequence %1 doesn&apos;t have any related annotations.</source>
        <translation>Failed to search for exon annotations. The sequence %1 doesn&apos;t have any related annotations.</translation>
    </message>
</context>
<context>
    <name>U2::GTest</name>
    <message>
        <location filename="../src/Primer3Tests.cpp" line="211"/>
        <source>Illegal TARGET value: %1</source>
        <translation type="unfinished">Illegal TARGET value: %1</translation>
    </message>
    <message>
        <location filename="../src/Primer3Tests.cpp" line="230"/>
        <location filename="../src/Primer3Tests.cpp" line="299"/>
        <source>Illegal PRIMER_DEFAULT_PRODUCT value: %1</source>
        <translation type="unfinished">Illegal PRIMER_DEFAULT_PRODUCT value: %1</translation>
    </message>
    <message>
        <location filename="../src/Primer3Tests.cpp" line="248"/>
        <source>Illegal PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION value: %1</source>
        <translation type="unfinished">Illegal PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION value: %1</translation>
    </message>
    <message>
        <location filename="../src/Primer3Tests.cpp" line="263"/>
        <source>Illegal INCLUDED_REGION value: %1</source>
        <translation type="unfinished">Illegal INCLUDED_REGION value: %1</translation>
    </message>
    <message>
        <location filename="../src/Primer3Tests.cpp" line="317"/>
        <source>Illegal EXCLUDED_REGION value: %1</source>
        <translation type="unfinished">Illegal EXCLUDED_REGION value: %1</translation>
    </message>
    <message>
        <location filename="../src/Primer3Tests.cpp" line="348"/>
        <source>Unrecognized PRIMER_TASK</source>
        <translation type="unfinished">Unrecognized PRIMER_TASK</translation>
    </message>
    <message>
        <location filename="../src/Primer3Tests.cpp" line="360"/>
        <source>Contradiction in primer_task definition</source>
        <translation type="unfinished">Contradiction in primer_task definition</translation>
    </message>
    <message>
        <location filename="../src/Primer3Tests.cpp" line="418"/>
        <source>Missing SEQUENCE tag</source>
        <translation type="unfinished">Missing SEQUENCE tag</translation>
    </message>
    <message>
        <location filename="../src/Primer3Tests.cpp" line="425"/>
        <source>Error in sequence quality data</source>
        <translation type="unfinished">Error in sequence quality data</translation>
    </message>
    <message>
        <location filename="../src/Primer3Tests.cpp" line="427"/>
        <source>Sequence quality data missing</source>
        <translation type="unfinished">Sequence quality data missing</translation>
    </message>
    <message>
        <location filename="../src/Primer3Tests.cpp" line="450"/>
        <source>PRIMER_PAIRS_NUMBER is incorrect. Expected:%2, but Actual:%3</source>
        <translation type="unfinished">PRIMER_PAIRS_NUMBER is incorrect. Expected:%2, but Actual:%3</translation>
    </message>
    <message>
        <location filename="../src/Primer3Tests.cpp" line="552"/>
        <source>%1 is incorrect. Expected:%2,%3, but Actual:NULL</source>
        <translation type="unfinished">%1 is incorrect. Expected:%2,%3, but Actual:NULL</translation>
    </message>
    <message>
        <location filename="../src/Primer3Tests.cpp" line="561"/>
        <source>%1 is incorrect. Expected:NULL, but Actual:%2,%3</source>
        <translation type="unfinished">%1 is incorrect. Expected:NULL, but Actual:%2,%3</translation>
    </message>
    <message>
        <location filename="../src/Primer3Tests.cpp" line="571"/>
        <source>%1 is incorrect. Expected:%2,%3, but Actual:%4,%5</source>
        <translation type="unfinished">%1 is incorrect. Expected:%2,%3, but Actual:%4,%5</translation>
    </message>
    <message>
        <location filename="../src/Primer3Tests.cpp" line="610"/>
        <location filename="../src/Primer3Tests.cpp" line="620"/>
        <location filename="../src/Primer3Tests.cpp" line="630"/>
        <source>%1 is incorrect. Expected:%2, but Actual:%3</source>
        <translation type="unfinished">%1 is incorrect. Expected:%2, but Actual:%3</translation>
    </message>
</context>
<context>
    <name>U2::Primer3ADVContext</name>
    <message>
        <location filename="../src/Primer3Plugin.cpp" line="93"/>
        <source>Primer3...</source>
        <translation>Primer3...</translation>
    </message>
    <message>
        <location filename="../src/Primer3Plugin.cpp" line="147"/>
        <source>Error</source>
        <translation>Error</translation>
    </message>
    <message>
        <location filename="../src/Primer3Plugin.cpp" line="147"/>
        <source>Cannot create an annotation object. Please check settings</source>
        <translation>Cannot create an annotation object. Please check settings</translation>
    </message>
</context>
<context>
    <name>U2::Primer3Dialog</name>
    <message>
        <location filename="../src/Primer3Dialog.cpp" line="83"/>
        <source>NONE</source>
        <translation>NONE</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.cpp" line="84"/>
        <source>HUMAN</source>
        <translation>HUMAN</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.cpp" line="85"/>
        <source>RODENT_AND_SIMPLE</source>
        <translation>RODENT_AND_SIMPLE</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.cpp" line="86"/>
        <source>RODENT</source>
        <translation>RODENT</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.cpp" line="87"/>
        <source>DROSOPHILA</source>
        <translation>DROSOPHILA</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.cpp" line="439"/>
        <source>Start Codon Position</source>
        <translation>Start Codon Position</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.cpp" line="453"/>
        <source>Product Min Tm</source>
        <translation>Product Min Tm</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.cpp" line="467"/>
        <source>Product Opt Tm</source>
        <translation>Product Opt Tm</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.cpp" line="481"/>
        <source>Product Max Tm</source>
        <translation>Product Max Tm</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.cpp" line="509"/>
        <source>Inside Penalty</source>
        <translation>Inside Penalty</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.cpp" line="523"/>
        <source>Internal Oligo Opt Tm</source>
        <translation>Internal Oligo Opt Tm</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.cpp" line="535"/>
        <source>Excluded Regions</source>
        <translation>Excluded Regions</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.cpp" line="547"/>
        <source>Internal Oligo Excluded Regions</source>
        <translation>Internal Oligo Excluded Regions</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.cpp" line="665"/>
        <source>Included region is too small for current product size ranges</source>
        <translation>Included region is too small for current product size ranges</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.cpp" line="672"/>
        <source>Product Size Ranges</source>
        <translation>Product Size Ranges</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.cpp" line="596"/>
        <source>Sequence Quality</source>
        <translation>Sequence Quality</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.cpp" line="698"/>
        <source>Cannot create an annotation object. Please check settings.</source>
        <translation type="unfinished"></translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.cpp" line="712"/>
        <source>Save primer settings</source>
        <translation>Save primer settings</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.cpp" line="794"/>
        <source>Load settings</source>
        <translation>Load settings</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.cpp" line="804"/>
        <source>Can not load settings file: invalid format.</source>
        <translation>Can not load settings file: invalid format.</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.cpp" line="495"/>
        <source>Opt GC%</source>
        <translation>Opt GC%</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.cpp" line="559"/>
        <source>Targets</source>
        <translation>Targets</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.cpp" line="603"/>
        <source>Sequence quality list length must be equal to the sequence length</source>
        <translation>Sequence quality list length must be equal to the sequence length</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.cpp" line="625"/>
        <source>Can&apos;t pick hyb oligo and only one primer</source>
        <translation>Can&apos;t pick hyb oligo and only one primer</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.cpp" line="645"/>
        <source>Nothing to pick</source>
        <translation>Nothing to pick</translation>
    </message>
    <message>
        <location filename="../src/Primer3Dialog.cpp" line="683"/>
        <source>The field &apos;%1&apos; has invalid value</source>
        <translation>The field &apos;%1&apos; has invalid value</translation>
    </message>
</context>
<context>
    <name>U2::Primer3Plugin</name>
    <message>
        <location filename="../src/Primer3Plugin.cpp" line="56"/>
        <source>Primer3</source>
        <translation>Primer3</translation>
    </message>
    <message>
        <location filename="../src/Primer3Plugin.cpp" line="56"/>
        <source>Integrated tool for PCR primers design.</source>
        <translation>Integrated tool for PCR primers design.</translation>
    </message>
</context>
<context>
    <name>U2::Primer3Task</name>
    <message>
        <location filename="../src/Primer3Task.cpp" line="371"/>
        <source>Pick primers task</source>
        <translation>Pick primers</translation>
    </message>
</context>
<context>
    <name>U2::Primer3ToAnnotationsTask</name>
    <message>
        <location filename="../src/Primer3Task.cpp" line="802"/>
        <source>Search primers to annotations</source>
        <translation>Search primers to annotations</translation>
    </message>
    <message>
        <location filename="../src/Primer3Task.cpp" line="834"/>
        <source>Failed to find any exon annotations associated with the sequence %1.Make sure the provided sequence is cDNA and has exonic structure annotated</source>
        <translation>Failed to find any exon annotations associated with the sequence %1.Make sure the provided sequence is cDNA and has exonic structure annotated</translation>
    </message>
    <message>
        <location filename="../src/Primer3Task.cpp" line="845"/>
        <source>The first exon from the selected range [%1,%2] is larger the number of exons (%2). Please set correct exon range.</source>
        <translation>The first exon from the selected range [%1,%2] is larger the number of exons (%2). Please set correct exon range.</translation>
    </message>
    <message>
        <location filename="../src/Primer3Task.cpp" line="851"/>
        <source>The the selected exon range [%1,%2] is larger the number of exons (%2). Please set correct exon range.</source>
        <translation>The the selected exon range [%1,%2] is larger the number of exons (%2). Please set correct exon range.</translation>
    </message>
</context>
<context>
    <name>U2::QDPrimerActor</name>
    <message>
        <location filename="../src/Primer3Query.cpp" line="73"/>
        <source>%1 invalid input. Excluded regions.</source>
        <translation>%1 invalid input. Excluded regions.</translation>
    </message>
    <message>
        <location filename="../src/Primer3Query.cpp" line="82"/>
        <source>%1 invalid input. Targets.</source>
        <translation>%1 invalid input. Targets.</translation>
    </message>
    <message>
        <location filename="../src/Primer3Query.cpp" line="91"/>
        <source>%1 invalid input. Product size ranges.</source>
        <translation>%1 invalid input. Product size ranges.</translation>
    </message>
</context>
</TS>
