LOCUS       ISP2_HEMAM               163 aa            linear   VRT 12-SEP-2018
DEFINITION  RecName: Full=Type-2 ice-structuring protein; AltName: Full=Type II
            antifreeze protein; Short=AFP; Flags: Precursor.
ACCESSION   P05140
VERSION     P05140.2
DBSOURCE    UniProtKB: locus ISP2_HEMAM, accession P05140;
            class: standard.
            created: Aug 13, 1987.
            sequence updated: Aug 1, 1990.
            annotation updated: Sep 12, 2018.
            xrefs: J02593.1, AAA49617.1, J05100.1, AAA49618.1, A34313, 2AFP_A
            xrefs (non-sequence databases): PDBsum:2AFP,
            ProteinModelPortal:P05140, SMR:P05140, HOVERGEN:HBG004151,
            EvolutionaryTrace:P05140, GO:0005576, GO:0030246,
            Gene3D:3.10.100.10, InterPro:IPR002353, InterPro:IPR001304,
            InterPro:IPR016186, InterPro:IPR018378, InterPro:IPR016187,
            Pfam:PF00059, PRINTS:PR00356, SMART:SM00034, SUPFAM:SSF56436,
            PROSITE:PS00615, PROSITE:PS50041
KEYWORDS    3D-structure; Antifreeze protein; Direct protein sequencing;
            Disulfide bond; Lectin; Secreted; Signal.
SOURCE      Hemitripterus americanus (sea raven)
  ORGANISM  Hemitripterus americanus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Perciformes; Cottioidei; Cottales;
            Agonidae; Hemitripterinae; Hemitripterus.
REFERENCE   1  (residues 1 to 163)
  AUTHORS   Ng,N.F., Trinh,K.Y. and Hew,C.L.
  TITLE     Structure of an antifreeze polypeptide precursor from the sea
            raven, Hemitripterus americanus
  JOURNAL   J. Biol. Chem. 261 (33), 15690-15695 (1986)
   PUBMED   3782083
  REMARK    NUCLEOTIDE SEQUENCE [MRNA].
REFERENCE   2  (residues 1 to 163)
  AUTHORS   Hayes,P.H., Scott,G.K., Ng,N.F., Hew,C.L. and Davies,P.L.
  TITLE     Cystine-rich type II antifreeze protein precursor is initiated from
            the third AUG codon of its mRNA
  JOURNAL   J. Biol. Chem. 264 (31), 18761-18767 (1989)
   PUBMED   2572595
  REMARK    NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], PROTEIN SEQUENCE OF
            36-41; 52-63; 95-97; 111-116 AND 121-137, SUBCELLULAR LOCATION, AND
            PTM.
REFERENCE   3  (residues 1 to 163)
  AUTHORS   Ng,N.F. and Hew,C.L.
  TITLE     Structure of an antifreeze polypeptide from the sea raven.
            Disulfide bonds and similarity to lectin-binding proteins
  JOURNAL   J. Biol. Chem. 267 (23), 16069-16075 (1992)
   PUBMED   1644794
  REMARK    PRELIMINARY DISULFIDE BONDS, AND SIMILARITY TO C-TYPE LECTINS.
REFERENCE   4  (residues 1 to 163)
  AUTHORS   Gronwald,W., Loewen,M.C., Lix,B., Daugulis,A.J., Sonnichsen,F.D.,
            Davies,P.L. and Sykes,B.D.
  TITLE     The solution structure of type II antifreeze protein reveals a new
            member of the lectin family
  JOURNAL   Biochemistry 37 (14), 4712-4721 (1998)
   PUBMED   9537986
  REMARK    STRUCTURE BY NMR, AND DISULFIDE BONDS.
COMMENT     On Nov 22, 2005 this sequence version replaced gi:1072445.
            [FUNCTION] Antifreeze proteins lower the blood freezing point.
            [SUBCELLULAR LOCATION] Secreted {ECO:0000269|PubMed:2572595}.
            [PTM] The N-terminus is blocked. {ECO:0000269|PubMed:2572595}.
            [SEQUENCE CAUTION] Sequence=AAA49617.1; Type=Erroneous initiation;
            Evidence={ECO:0000305}.
FEATURES             Location/Qualifiers
     source          1..163
                     /organism="Hemitripterus americanus"
                     /db_xref="taxon:8094"
     Protein         1..163
                     /product="Type-2 ice-structuring protein"
                     /note="Type II antifreeze protein; AFP"
                     /UniProtKB_evidence="Evidence at protein level"
     Region          1..17
                     /region_name="Signal"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="{ECO:0000255}."
     Region          18..34
                     /region_name="Propeptide"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="{ECO:0000305|PubMed:2572595}.
                     /FTId=PRO_0000017544."
     Region          35..163
                     /region_name="Mature chain"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Type-2 ice-structuring protein.
                     {ECO:0000305|PubMed:2572595}. /FTId=PRO_0000017545."
     Region          38
                     /region_name="Conflict"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="P -> G (in Ref. 2; AAA49618). {ECO:0000305}."
     Region          39..163
                     /region_name="Domain"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="C-type lectin.
                     {ECO:0000255|PROSITE-ProRule:PRU00040}."
     Region          41..159
                     /region_name="CLECT"
                     /note="C-type lectin (CTL) or carbohydrate-recognition
                     domain (CRD); smart00034"
                     /db_xref="CDD:214480"
     Bond            bond(41,52)
                     /bond_type="disulfide"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="{ECO:0000255|PROSITE-ProRule:PRU00040,
                     ECO:0000269|PubMed:9537986}."
     Region          43..45
                     /region_name="Beta-strand region"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="{ECO:0000244|PDB:2AFP}."
     Region          49..53
                     /region_name="Beta-strand region"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="{ECO:0000244|PDB:2AFP}."
     Region          62..72
                     /region_name="Helical region"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="{ECO:0000244|PDB:2AFP}."
     Bond            bond(69,159)
                     /bond_type="disulfide"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="{ECO:0000255|PROSITE-ProRule:PRU00040,
                     ECO:0000269|PubMed:9537986}."
     Region          80..82
                     /region_name="Beta-strand region"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="{ECO:0000244|PDB:2AFP}."
     Region          83..91
                     /region_name="Helical region"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="{ECO:0000244|PDB:2AFP}."
     Bond            bond(103,134)
                     /bond_type="disulfide"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="{ECO:0000255|PROSITE-ProRule:PRU00040,
                     ECO:0000269|PubMed:9537986}."
     Region          103..107
                     /region_name="Beta-strand region"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="{ECO:0000244|PDB:2AFP}."
     Region          110..114
                     /region_name="Beta-strand region"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="{ECO:0000244|PDB:2AFP}."
     Region          122..124
                     /region_name="Beta-strand region"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="{ECO:0000244|PDB:2AFP}."
     Bond            bond(123,145)
                     /bond_type="disulfide"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="{ECO:0000255|PROSITE-ProRule:PRU00040,
                     ECO:0000269|PubMed:9537986}."
     Site            order(133,137,139,145,147..150,153..155)
                     /site_type="other"
                     /note="ligand binding surface [chemical binding]"
                     /db_xref="CDD:153057"
     Bond            bond(135,151)
                     /bond_type="disulfide"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="{ECO:0000255|PROSITE-ProRule:PRU00040,
                     ECO:0000269|PubMed:9537986}."
     Region          141..144
                     /region_name="Beta-strand region"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="{ECO:0000244|PDB:2AFP}."
     Region          159..161
                     /region_name="Beta-strand region"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="{ECO:0000244|PDB:2AFP}."
ORIGIN      
        1 mltvsllvca mmaltqandd kilkgtatea gpvsqrappn cpagwqplgd rciyyettam
       61 twalaetncm klgghlasih sqeehsfiqt lnagvvwigg saclqagawt wsdgtpmnfr
      121 swcstkpddv laaccmqmta aadqcwddlp cpashksvca mtf
//

LOCUS       AAA49617                 195 aa            linear   VRT 28-APR-1993
DEFINITION  antifreeze polypeptide (AFP) precursor [Hemitripterus americanus].
ACCESSION   AAA49617
VERSION     AAA49617.1
DBSOURCE    locus SRAAFP accession J02593.1
KEYWORDS    .
SOURCE      Hemitripterus americanus (sea raven)
  ORGANISM  Hemitripterus americanus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Perciformes; Cottioidei; Cottales;
            Agonidae; Hemitripterinae; Hemitripterus.
REFERENCE   1  (residues 1 to 195)
  AUTHORS   Ng,N.F., Trinh,K.Y. and Hew,C.L.
  TITLE     Structure of an antifreeze polypeptide precursor from the sea
            raven, Hemitripterus americanus
  JOURNAL   J. Biol. Chem. 261 (33), 15690-15695 (1986)
   PUBMED   3782083
COMMENT     Draft entry and printed copy of the sequence [1] kindly provided by
            N.F.Ng, 11-AUG-1986.
            Method: conceptual translation.
FEATURES             Location/Qualifiers
     source          1..195
                     /organism="Hemitripterus americanus"
                     /db_xref="taxon:8094"
     Protein         1..195
                     /name="antifreeze polypeptide (AFP) precursor"
     sig_peptide     1..29
                     /note="antifreeze polypeptide (AFP) signal peptide"
     mat_peptide     30..195
                     /product="antifreeze polypeptide (AFP)"
     Region          73..191
                     /region_name="CLECT"
                     /note="C-type lectin (CTL) or carbohydrate-recognition
                     domain (CRD); smart00034"
                     /db_xref="CDD:214480"
     Site            order(165,169,171,177,179..182,185..187)
                     /site_type="other"
                     /note="ligand binding surface [chemical binding]"
                     /db_xref="CDD:153057"
     CDS             1..195
                     /coded_by="J02593.1:10..597"
ORIGIN      
        1 mqrqqadtet redistagls iifivctist trmltvsllv cammaltqan ddkilkgtat
       61 eagpvsqrap pncpagwqpl gdrciyyett amtwalaetn cmklgghlas ihsqeehsfi
      121 qtlnagvvwi ggsaclqaga wtwsdgtpmn frswcstkpd dvlaaccmqm taaadqcwdd
      181 lpcpashksv camtf
//

LOCUS       AAA49618                 163 aa            linear   VRT 28-APR-1993
DEFINITION  antifreeze protein [Hemitripterus americanus].
ACCESSION   AAA49618
VERSION     AAA49618.1
DBSOURCE    locus SRAAFPG accession J05100.1
KEYWORDS    .
SOURCE      Hemitripterus americanus (sea raven)
  ORGANISM  Hemitripterus americanus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Perciformes; Cottioidei; Cottales;
            Agonidae; Hemitripterinae; Hemitripterus.
REFERENCE   1  (residues 1 to 163)
  AUTHORS   Hayes,P.H., Scott,G.K., Ng,N.F., Hew,C.L. and Davies,P.L.
  TITLE     Cystine-rich type II antifreeze protein precursor is initiated from
            the third AUG codon of its mRNA
  JOURNAL   J. Biol. Chem. 264 (31), 18761-18767 (1989)
   PUBMED   2572595
COMMENT     Draft entry and computer-readable sequence for [1] kindly submitted
            by P.L.Davies, 19-OCT-1989.
            Method: conceptual translation.
FEATURES             Location/Qualifiers
     source          1..163
                     /organism="Hemitripterus americanus"
                     /db_xref="taxon:8094"
     Protein         1..163
                     /name="antifreeze protein"
     Region          41..159
                     /region_name="CLECT"
                     /note="C-type lectin (CTL) or carbohydrate-recognition
                     domain (CRD); smart00034"
                     /db_xref="CDD:214480"
     Site            order(133,137,139,145,147..150,153..155)
                     /site_type="other"
                     /note="ligand binding surface [chemical binding]"
                     /db_xref="CDD:153057"
     CDS             1..163
                     /coded_by="join(J05100.1:434..494,J05100.1:1246..1382,
                     J05100.1:1488..1604,J05100.1:1697..1805,
                     J05100.1:2045..2112)"
ORIGIN      
        1 mltvsllvca mmaltqandd kilkgtatea gpvsqragpn cpagwqplgd rciyyettam
       61 twalaetncm klgghlasih sqeehsfiqt lnagvvwigg saclqagawt wsdgtpmnfr
      121 swcstkpddv laaccmqmta aadqcwddlp cpashksvca mtf
//

LOCUS       2AFP_A                   129 aa            linear   VRT 10-OCT-2012
DEFINITION  Chain A, The Solution Structure Of Type Ii Antifreeze Protein
            Reveals A New Member Of The Lectin Family.
ACCESSION   2AFP_A
VERSION     2AFP_A
DBSOURCE    pdb: molecule 2AFP, chain 65, release Jun 18, 2009;
            deposition: Dec 14, 1998;
            class: Antifreeze Protein;
            source: Mmdb_id: 12071, Pdb_id 1: 2AFP;
            Exp. method: Solution Nmr.
KEYWORDS    .
SOURCE      Hemitripterus americanus (sea raven)
  ORGANISM  Hemitripterus americanus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Perciformes; Cottioidei; Cottales;
            Agonidae; Hemitripterinae; Hemitripterus.
REFERENCE   1  (residues 1 to 129)
  AUTHORS   Gronwald,W., Loewen,M.C., Lix,B., Daugulis,A.J., Sonnichsen,F.D.,
            Davies,P.L. and Sykes,B.D.
  TITLE     The solution structure of type II antifreeze protein reveals a new
            member of the lectin family
  JOURNAL   Biochemistry 37 (14), 4712-4721 (1998)
   PUBMED   9537986
REFERENCE   2  (residues 1 to 129)
  AUTHORS   Gronwald,W., Loewen,M.C., Lix,B., Daugulis,A.J., Sonnichsen,F.D.,
            Davies,P.L. and Sykes,B.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-1998)
COMMENT     SEQRES.
FEATURES             Location/Qualifiers
     source          1..129
                     /organism="Hemitripterus americanus"
                     /db_xref="taxon:8094"
     Region          7..125
                     /region_name="CLECT"
                     /note="C-type lectin (CTL) or carbohydrate-recognition
                     domain (CRD); smart00034"
                     /db_xref="CDD:214480"
     Bond            bond(7,18)
                     /bond_type="disulfide"
     SecStr          12..15
                     /sec_str_type="sheet"
                     /note="strand 1"
     SecStr          16..23
                     /sec_str_type="sheet"
                     /note="strand 2"
     SecStr          28..38
                     /sec_str_type="helix"
                     /note="helix 1"
     Bond            bond(35,125)
                     /bond_type="disulfide"
     SecStr          49..56
                     /sec_str_type="helix"
                     /note="helix 2"
     SecStr          60..63
                     /sec_str_type="sheet"
                     /note="strand 3"
     Bond            bond(69,100)
                     /bond_type="disulfide"
     Bond            bond(89,111)
                     /bond_type="disulfide"
     Site            order(99,103,105,111,113..116,119..121)
                     /site_type="other"
                     /note="ligand binding surface [chemical binding]"
                     /db_xref="CDD:153057"
     Bond            bond(101,117)
                     /bond_type="disulfide"
     SecStr          101..107
                     /sec_str_type="sheet"
                     /note="strand 4"
     SecStr          109..116
                     /sec_str_type="sheet"
                     /note="strand 5"
     SecStr          122..128
                     /sec_str_type="sheet"
                     /note="strand 6"
ORIGIN      
        1 qragpncpag wqplgdrciy yettamtwal aetncmklgg hlasihsqee hsfiqtlnag
       61 vvwiggsacl qagawtwsdg tpmnfrswcs tkpddvlaac cmqmtaaadq cwddlpcpas
      121 hksvcamtf
//

LOCUS       ABA41369                 147 aa            linear   VRT 06-JUL-2006
DEFINITION  type II antifreeze protein [Clupea harengus].
ACCESSION   ABA41369
VERSION     ABA41369.1
DBSOURCE    accession DQ062447.1
KEYWORDS    .
SOURCE      Clupea harengus (Atlantic herring)
  ORGANISM  Clupea harengus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Clupei; Clupeiformes;
            Clupeoidei; Clupeidae; Clupea.
REFERENCE   1  (residues 1 to 147)
  AUTHORS   Cheng,C.H., Cziko,P.A. and Evans,C.W.
  TITLE     Nonhepatic origin of notothenioid antifreeze reveals pancreatic
            synthesis as common mechanism in polar fish freezing avoidance
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 103 (27), 10491-10496 (2006)
   PUBMED   16798878
REFERENCE   2  (residues 1 to 147)
  AUTHORS   Cheng,C.-H.C., Logue,J.A., Cziko,P.A. and Chen,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2005) Animal Biology, University of Illinois, 515
            Morrill Hall, 505 S. Goodwin Ave., Urbana, IL 61801, USA
COMMENT     Method: conceptual translation supplied by author.
FEATURES             Location/Qualifiers
     source          1..147
                     /organism="Clupea harengus"
                     /db_xref="taxon:7950"
                     /clone="13"
                     /tissue_type="pancreas"
                     /dev_stage="adult"
     Protein         1..147
                     /product="type II antifreeze protein"
     Region          21..142
                     /region_name="CLECT"
                     /note="C-type lectin (CTL) or carbohydrate-recognition
                     domain (CRD); smart00034"
                     /db_xref="CDD:214480"
     Site            order(116,120,122,128,130..133,136..138)
                     /site_type="other"
                     /note="ligand binding surface [chemical binding]"
                     /db_xref="CDD:153057"
     CDS             1..147
                     /gene="AFPII"
                     /coded_by="DQ062447.1:10..453"
ORIGIN      
        1 mltvsllvca ivaltraade cpsdwkmfng rcflfnplql hwadaqescm kegaylasih
       61 sleestfvke ltsadlipsw iggtgcqist rwfwmdstsm dyadwcaaqp dttltecciq
      121 mnvgigkcwn dtpcthlhss icakplk
//

LOCUS       2ZIB_A                   133 aa            linear   VRT 10-OCT-2012
DEFINITION  Chain A, Type Ii Antifreeze Protein.
ACCESSION   2ZIB_A
VERSION     2ZIB_A
DBSOURCE    pdb: molecule 2ZIB, chain 65, release Aug 28, 2012;
            deposition: Feb 14, 2008;
            class: Antifreeze Protein;
            source: Mmdb_id: 66146, Pdb_id 1: 2ZIB;
            Exp. method: X-Ray Diffraction.
KEYWORDS    .
SOURCE      Brachyopsis segaliensis
  ORGANISM  Brachyopsis segaliensis
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Perciformes; Cottioidei; Cottales;
            Agonidae; Brachyopsinae; Brachyopsis.
REFERENCE   1  (residues 1 to 133)
  AUTHORS   Nishimiya,Y., Kondo,H., Takamichi,M., Sugimoto,H., Suzuki,M.,
            Miura,A. and Tsuda,S.
  TITLE     Crystal structure and mutational analysis of Ca2+-independent type
            II antifreeze protein from longsnout poacher, Brachyopsis rostratus
  JOURNAL   J. Mol. Biol. 382 (3), 734-746 (2008)
   PUBMED   18674542
REFERENCE   2  (residues 1 to 133)
  AUTHORS   Nishimiya,Y., Sato,R., Kondo,H., Noro,N., Sugimoto,H., Suzuki,M.
            and Tsuda,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-FEB-2008)
COMMENT     Crystal Structure Analysis Of Calcium-Independent Type Ii
            Antifreeze Protein.
FEATURES             Location/Qualifiers
     source          1..133
                     /organism="Brachyopsis segaliensis"
                     /db_xref="taxon:1633465"
     Region          10..128
                     /region_name="CLECT"
                     /note="C-type lectin (CTL) or carbohydrate-recognition
                     domain (CRD); smart00034"
                     /db_xref="CDD:214480"
     Bond            bond(10,21)
                     /bond_type="disulfide"
     SecStr          14..18
                     /sec_str_type="sheet"
                     /note="strand 1"
     SecStr          19..26
                     /sec_str_type="sheet"
                     /note="strand 2"
     SecStr          27..30
                     /sec_str_type="sheet"
                     /note="strand 3"
     SecStr          31..41
                     /sec_str_type="helix"
                     /note="helix 1"
     Bond            bond(38,128)
                     /bond_type="disulfide"
     SecStr          51..60
                     /sec_str_type="helix"
                     /note="helix 2"
     SecStr          63..67
                     /sec_str_type="sheet"
                     /note="strand 4"
     SecStr          68..71
                     /sec_str_type="sheet"
                     /note="strand 5"
     Bond            bond(72,103)
                     /bond_type="disulfide"
     SecStr          77..81
                     /sec_str_type="sheet"
                     /note="strand 6"
     Bond            bond(92,114)
                     /bond_type="disulfide"
     Site            order(102,106,108,114,116..119,122..124)
                     /site_type="other"
                     /note="ligand binding surface [chemical binding]"
                     /db_xref="CDD:153057"
     SecStr          103..109
                     /sec_str_type="sheet"
                     /note="strand 7"
     Bond            bond(104,120)
                     /bond_type="disulfide"
     SecStr          114..119
                     /sec_str_type="sheet"
                     /note="strand 8"
     SecStr          122..131
                     /sec_str_type="sheet"
                     /note="strand 9"
ORIGIN      
        1 hhhhhhalvc pagwtlhgqr cfyseatamt wdlaeancvn kgghlasihs leeqlyikdi
       61 vagivwiggs ackvagawsw tdgtpvdyrt wcptkpndil sdccmqmtaa vdkcwddlpc
      121 pashasicak aai
//

LOCUS       ACI32416                 165 aa            linear   VRT 01-MAR-2010
DEFINITION  antifreeze protein [Siniperca chuatsi].
ACCESSION   ACI32416
VERSION     ACI32416.1
DBSOURCE    accession EU719616.1
KEYWORDS    .
SOURCE      Siniperca chuatsi (mandarin fish)
  ORGANISM  Siniperca chuatsi
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Centrarchiformes; Centrarchoidei;
            Sinipercidae; Siniperca.
REFERENCE   1  (residues 1 to 165)
  AUTHORS   Li,G.G., Liang,X.F. and Li,G.Z.
  TITLE     Molecular characterization of antifreeze protein
  JOURNAL   Unpublished
REFERENCE   2  (residues 1 to 165)
  AUTHORS   Li,G.G., Liang,X.F. and Li,G.Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-MAY-2008) Biology, Jinan University, Shipai,
            Guangzhou, Guangdong 510632, China
FEATURES             Location/Qualifiers
     source          1..165
                     /organism="Siniperca chuatsi"
                     /db_xref="taxon:119488"
     Protein         1..165
                     /product="antifreeze protein"
                     /name="AFP"
     Region          44..162
                     /region_name="CLECT"
                     /note="C-type lectin (CTL) or carbohydrate-recognition
                     domain (CRD); smart00034"
                     /db_xref="CDD:214480"
     Site            order(136,140,142,148,150..153,156..158)
                     /site_type="other"
                     /note="ligand binding surface [chemical binding]"
                     /db_xref="CDD:153057"
     CDS             1..165
                     /coded_by="EU719616.1:64..561"
ORIGIN      
        1 mltvsllvca mmaltrange edtsingtvt ndtnidvlna rngcppgwep fdgrcfklvt
       61 srltwakaek ncqafggnla strnsedynf iqqmtteltw iggsacqetn awfwsdgtpm
      121 dkplwcagqp dgalaqcclq intgdgkcwd dqpcrnllps vcikk
//

LOCUS       XP_003455917             158 aa            linear   VRT 26-JUL-2018
DEFINITION  ladderlectin-like [Oreochromis niloticus].
ACCESSION   XP_003455917
VERSION     XP_003455917.1
DBLINK      BioProject: PRJNA354796
DBSOURCE    REFSEQ: accession XM_003455869.4
KEYWORDS    RefSeq.
SOURCE      Oreochromis niloticus (Nile tilapia)
  ORGANISM  Oreochromis niloticus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Ovalentaria; Cichlomorphae; Cichliformes;
            Cichlidae; African cichlids; Pseudocrenilabrinae; Oreochromini;
            Oreochromis.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_031967.2) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI
            Annotation Status           :: Full annotation
            Annotation Name             :: Oreochromis niloticus Annotation
                                           Release 104
            Annotation Version          :: 104
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 8.1
            Annotation Method           :: Best-placed RefSeq; Gnomon
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..158
                     /organism="Oreochromis niloticus"
                     /isolate="F11D_XX"
                     /db_xref="taxon:8128"
                     /sex="female"
                     /tissue_type="blood"
                     /dev_stage="adult"
                     /linkage_group="LG3"
     Protein         1..158
                     /product="ladderlectin-like"
                     /calculated_mol_wt=17695
     Region          37..149
                     /region_name="CLECT"
                     /note="C-type lectin (CTL)/C-type lectin-like (CTLD)
                     domain; cd00037"
                     /db_xref="CDD:153057"
     Site            order(123,127,129,135,137..140,143..145)
                     /site_type="other"
                     /note="ligand binding surface [chemical binding]"
                     /db_xref="CDD:153057"
     CDS             1..158
                     /gene="LOC100694817"
                     /coded_by="XM_003455869.4:163..639"
                     /db_xref="GeneID:100694817"
ORIGIN      
        1 mklltvsall cvmmalstva arrcrgcpqg wtrirrrcfl yvpramnwaa aernclsmgg
       61 nlasvhtste yhliqrltah ngygvtwvgg tdapgegiwl wsdgsrfnyq nwcggepnny
      121 lkqdclqinf sgskcwddqh chvnlpsica rkisrrrg
//

LOCUS       XP_013121247             205 aa            linear   VRT 26-JUL-2018
DEFINITION  type-2 ice-structuring protein [Oreochromis niloticus].
ACCESSION   XP_013121247
VERSION     XP_013121247.1
DBLINK      BioProject: PRJNA354796
DBSOURCE    REFSEQ: accession XM_013265793.3
KEYWORDS    RefSeq.
SOURCE      Oreochromis niloticus (Nile tilapia)
  ORGANISM  Oreochromis niloticus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Ovalentaria; Cichlomorphae; Cichliformes;
            Cichlidae; African cichlids; Pseudocrenilabrinae; Oreochromini;
            Oreochromis.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_031967.2) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI
            Annotation Status           :: Full annotation
            Annotation Name             :: Oreochromis niloticus Annotation
                                           Release 104
            Annotation Version          :: 104
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 8.1
            Annotation Method           :: Best-placed RefSeq; Gnomon
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Oreochromis niloticus"
                     /isolate="F11D_XX"
                     /db_xref="taxon:8128"
                     /sex="female"
                     /tissue_type="blood"
                     /dev_stage="adult"
                     /linkage_group="LG3"
     Protein         1..205
                     /product="type-2 ice-structuring protein"
                     /calculated_mol_wt=23320
     Region          89..203
                     /region_name="CLECT"
                     /note="C-type lectin (CTL)/C-type lectin-like (CTLD)
                     domain; cd00037"
                     /db_xref="CDD:153057"
     Site            order(175,179,181,187,189..192,195..197)
                     /site_type="other"
                     /note="ligand binding surface [chemical binding]"
                     /db_xref="CDD:153057"
     CDS             1..205
                     /gene="LOC100709017"
                     /coded_by="XM_013265793.3:469..1086"
                     /db_xref="GeneID:100709017"
ORIGIN      
        1 mqickykqyi naglrevqhl htgqlklpad tekkkekiff krdtnitmkl ltvsallcam
       61 maltmavaks hlvkmsngcp hgwtrhsdrc fyyvptamsw araernclsm ganlasvrss
      121 reyqavqslt ahhgysetwi ggtdapqegi wlwsdgtsfh yshwcpgepn nlfnqhcikm
      181 nhgdskcwdd mwcdhhrpsv cakkv
//

LOCUS       XP_018559038             174 aa            linear   VRT 14-OCT-2016
DEFINITION  PREDICTED: type-2 ice-structuring protein-like isoform X1 [Lates
            calcarifer].
ACCESSION   XP_018559038
VERSION     XP_018559038.1
DBLINK      BioProject: PRJNA345597
DBSOURCE    REFSEQ: accession XM_018703522.1
KEYWORDS    RefSeq.
SOURCE      Lates calcarifer (barramundi perch)
  ORGANISM  Lates calcarifer
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Carangaria; Carangaria incertae sedis;
            Centropomidae; Lates.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_017363843.1) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI
            Annotation Status           :: Full annotation
            Annotation Version          :: Lates calcarifer Annotation Release
                                           100
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 7.2
            Annotation Method           :: Best-placed RefSeq; Gnomon
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..174
                     /organism="Lates calcarifer"
                     /isolate="ASB-BC8"
                     /db_xref="taxon:8187"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Brain, Kidney"
                     /dev_stage="adult"
                     /note="Back-crossed individual (decreased polymorphism)"
     Protein         1..174
                     /product="type-2 ice-structuring protein-like isoform X1"
                     /calculated_mol_wt=20086
     CDS             1..174
                     /gene="LOC108901866"
                     /coded_by="XM_018703522.1:42..566"
                     /db_xref="GeneID:108901866"
ORIGIN      
        1 mltvwvlvca mmaltraval peekakkddq avetdlvkrt yygcssgwsr fnrrcfhfvp
       61 kpmtwaqaer ncrsmggnla svhsvqeyhe iqrlimtaty eykaawlggs daqeenvwlw
      121 sdgrpfhysn wchgepnnyr rqqncmqmnh aaqkcwddlq cnahlpsica krgy
//

LOCUS       XP_018559046             173 aa            linear   VRT 14-OCT-2016
DEFINITION  PREDICTED: type-2 ice-structuring protein-like isoform X2 [Lates
            calcarifer].
ACCESSION   XP_018559046
VERSION     XP_018559046.1
DBLINK      BioProject: PRJNA345597
DBSOURCE    REFSEQ: accession XM_018703530.1
KEYWORDS    RefSeq.
SOURCE      Lates calcarifer (barramundi perch)
  ORGANISM  Lates calcarifer
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Carangaria; Carangaria incertae sedis;
            Centropomidae; Lates.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_017363843.1) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI
            Annotation Status           :: Full annotation
            Annotation Version          :: Lates calcarifer Annotation Release
                                           100
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 7.2
            Annotation Method           :: Best-placed RefSeq; Gnomon
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..173
                     /organism="Lates calcarifer"
                     /isolate="ASB-BC8"
                     /db_xref="taxon:8187"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Brain, Kidney"
                     /dev_stage="adult"
                     /note="Back-crossed individual (decreased polymorphism)"
     Protein         1..173
                     /product="type-2 ice-structuring protein-like isoform X2"
                     /calculated_mol_wt=19987
     CDS             1..173
                     /gene="LOC108901866"
                     /coded_by="XM_018703530.1:42..563"
                     /db_xref="GeneID:108901866"
ORIGIN      
        1 mltvwvlvca mmaltraval peekakkddq aetdlvkrty ygcssgwsrf nrrcfhfvpk
       61 pmtwaqaern crsmggnlas vhsvqeyhei qrlimtatye ykaawlggsd aqeenvwlws
      121 dgrpfhysnw chgepnnyrr qqncmqmnha aqkcwddlqc nahlpsicak rgy
//

LOCUS       XP_018535855             192 aa            linear   VRT 14-OCT-2016
DEFINITION  PREDICTED: type-2 ice-structuring protein-like [Lates calcarifer].
ACCESSION   XP_018535855
VERSION     XP_018535855.1
DBLINK      BioProject: PRJNA345597
DBSOURCE    REFSEQ: accession XM_018680339.1
KEYWORDS    RefSeq.
SOURCE      Lates calcarifer (barramundi perch)
  ORGANISM  Lates calcarifer
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Carangaria; Carangaria incertae sedis;
            Centropomidae; Lates.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_017363954.1) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI
            Annotation Status           :: Full annotation
            Annotation Version          :: Lates calcarifer Annotation Release
                                           100
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 7.2
            Annotation Method           :: Best-placed RefSeq; Gnomon
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..192
                     /organism="Lates calcarifer"
                     /isolate="ASB-BC8"
                     /db_xref="taxon:8187"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Brain, Kidney"
                     /dev_stage="adult"
                     /note="Back-crossed individual (decreased polymorphism)"
     Protein         1..192
                     /product="type-2 ice-structuring protein-like"
                     /calculated_mol_wt=20563
     CDS             1..192
                     /gene="LOC108885853"
                     /coded_by="XM_018680339.1:87..665"
                     /db_xref="GeneID:108885853"
ORIGIN      
        1 mltvsllvca vmalasaddn sttsivdvsv aednsttsld vnaaednstt skkeevapsc
       61 eigwsefngr cflfvstems wadaekncll kkghlasvhn eeeykhiqav vnahtgghpt
      121 twvggsdcqk egiwlwsdgs gfefdswceg epdnyvgaes clqinanesh cwndfpcstv
      181 lpsvcasvpq ss
//

LOCUS       XP_018535857             179 aa            linear   VRT 14-OCT-2016
DEFINITION  PREDICTED: type-2 ice-structuring protein-like [Lates calcarifer].
ACCESSION   XP_018535857
VERSION     XP_018535857.1
DBLINK      BioProject: PRJNA345597
DBSOURCE    REFSEQ: accession XM_018680341.1
KEYWORDS    RefSeq.
SOURCE      Lates calcarifer (barramundi perch)
  ORGANISM  Lates calcarifer
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Carangaria; Carangaria incertae sedis;
            Centropomidae; Lates.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_017363954.1) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI
            Annotation Status           :: Full annotation
            Annotation Version          :: Lates calcarifer Annotation Release
                                           100
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 7.2
            Annotation Method           :: Best-placed RefSeq; Gnomon
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..179
                     /organism="Lates calcarifer"
                     /isolate="ASB-BC8"
                     /db_xref="taxon:8187"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Brain, Kidney"
                     /dev_stage="adult"
                     /note="Back-crossed individual (decreased polymorphism)"
     Protein         1..179
                     /product="type-2 ice-structuring protein-like"
                     /calculated_mol_wt=19176
     CDS             1..179
                     /gene="LOC108885856"
                     /coded_by="XM_018680341.1:89..628"
                     /db_xref="GeneID:108885856"
ORIGIN      
        1 mltvsllvca mmalasaddn sttslvdvsv aeddsttstk ekaapsceig wsefngrcfl
       61 fvstemswad aekncllkkg hlasvhneee ykhiqavvna htgghpatwv ggsdcqkegi
      121 wlwsdgssfe fnswcegepd nfagaesclq inaneshcwn dfpcstvlps vcasvpqss
//

LOCUS       XP_018535874             174 aa            linear   VRT 14-OCT-2016
DEFINITION  PREDICTED: type-2 ice-structuring protein-like isoform X1 [Lates
            calcarifer].
ACCESSION   XP_018535874
VERSION     XP_018535874.1
DBLINK      BioProject: PRJNA345597
DBSOURCE    REFSEQ: accession XM_018680358.1
KEYWORDS    RefSeq.
SOURCE      Lates calcarifer (barramundi perch)
  ORGANISM  Lates calcarifer
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Carangaria; Carangaria incertae sedis;
            Centropomidae; Lates.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_017363958.1) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI
            Annotation Status           :: Full annotation
            Annotation Version          :: Lates calcarifer Annotation Release
                                           100
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 7.2
            Annotation Method           :: Best-placed RefSeq; Gnomon
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..174
                     /organism="Lates calcarifer"
                     /isolate="ASB-BC8"
                     /db_xref="taxon:8187"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Brain, Kidney"
                     /dev_stage="adult"
                     /note="Back-crossed individual (decreased polymorphism)"
     Protein         1..174
                     /product="type-2 ice-structuring protein-like isoform X1"
                     /calculated_mol_wt=20090
     CDS             1..174
                     /gene="LOC108885873"
                     /coded_by="XM_018680358.1:57..581"
                     /db_xref="GeneID:108885873"
ORIGIN      
        1 mltvwvlvca mmaltraaal peekakkddq avetdlvkrt yygcssgwsr fnrrcfhfvp
       61 kpmtwaqaer ncrsmggnla svhsmqeyhe iqrlimtaty eykaawlggs daqeenvwlw
      121 sdgrpfhysn wchgepnnyr rqqncmqmnh aaqkcwddlq cnahlpsica krgy
//

LOCUS       XP_018535875             173 aa            linear   VRT 14-OCT-2016
DEFINITION  PREDICTED: type-2 ice-structuring protein-like isoform X2 [Lates
            calcarifer].
ACCESSION   XP_018535875
VERSION     XP_018535875.1
DBLINK      BioProject: PRJNA345597
DBSOURCE    REFSEQ: accession XM_018680359.1
KEYWORDS    RefSeq.
SOURCE      Lates calcarifer (barramundi perch)
  ORGANISM  Lates calcarifer
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Carangaria; Carangaria incertae sedis;
            Centropomidae; Lates.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_017363958.1) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI
            Annotation Status           :: Full annotation
            Annotation Version          :: Lates calcarifer Annotation Release
                                           100
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 7.2
            Annotation Method           :: Best-placed RefSeq; Gnomon
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..173
                     /organism="Lates calcarifer"
                     /isolate="ASB-BC8"
                     /db_xref="taxon:8187"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Brain, Kidney"
                     /dev_stage="adult"
                     /note="Back-crossed individual (decreased polymorphism)"
     Protein         1..173
                     /product="type-2 ice-structuring protein-like isoform X2"
                     /calculated_mol_wt=19991
     CDS             1..173
                     /gene="LOC108885873"
                     /coded_by="XM_018680359.1:4..525"
                     /db_xref="GeneID:108885873"
ORIGIN      
        1 mltvwvlvca mmaltraaal peekakkddq aetdlvkrty ygcssgwsrf nrrcfhfvpk
       61 pmtwaqaern crsmggnlas vhsmqeyhei qrlimtatye ykaawlggsd aqeenvwlws
      121 dgrpfhysnw chgepnnyrr qqncmqmnha aqkcwddlqc nahlpsicak rgy
//

LOCUS       XP_018559775             205 aa            linear   VRT 14-OCT-2016
DEFINITION  PREDICTED: type-2 ice-structuring protein-like isoform X1 [Lates
            calcarifer].
ACCESSION   XP_018559775
VERSION     XP_018559775.1
DBLINK      BioProject: PRJNA345597
DBSOURCE    REFSEQ: accession XM_018704259.1
KEYWORDS    RefSeq.
SOURCE      Lates calcarifer (barramundi perch)
  ORGANISM  Lates calcarifer
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Carangaria; Carangaria incertae sedis;
            Centropomidae; Lates.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_017366387.1) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI
            Annotation Status           :: Full annotation
            Annotation Version          :: Lates calcarifer Annotation Release
                                           100
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 7.2
            Annotation Method           :: Best-placed RefSeq; Gnomon
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Lates calcarifer"
                     /isolate="ASB-BC8"
                     /db_xref="taxon:8187"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Brain, Kidney"
                     /dev_stage="adult"
                     /note="Back-crossed individual (decreased polymorphism)"
     Protein         1..205
                     /product="type-2 ice-structuring protein-like isoform X1"
                     /calculated_mol_wt=21905
     CDS             1..205
                     /gene="LOC108902415"
                     /coded_by="XM_018704259.1:95..712"
                     /db_xref="GeneID:108902415"
ORIGIN      
        1 mltvsllvca vmalasaddn sttsivdvsv aednsttsld vnaaednstt sldvnaaedd
       61 sttskkeeva psceigwsef ngrcflfvst emswadaekn clhkkghlas vhneeeykhi
      121 qavvnahtgg hpttwvggsd cqkegiwlws dgsgfefdsw cegqpdnyvg aesclqinan
      181 eshcwndfpc stvlpsvcas vpqss
//

LOCUS       XP_018559776             192 aa            linear   VRT 14-OCT-2016
DEFINITION  PREDICTED: type-2 ice-structuring protein-like isoform X2 [Lates
            calcarifer].
ACCESSION   XP_018559776
VERSION     XP_018559776.1
DBLINK      BioProject: PRJNA345597
DBSOURCE    REFSEQ: accession XM_018704260.1
KEYWORDS    RefSeq.
SOURCE      Lates calcarifer (barramundi perch)
  ORGANISM  Lates calcarifer
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Carangaria; Carangaria incertae sedis;
            Centropomidae; Lates.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_017366387.1) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI
            Annotation Status           :: Full annotation
            Annotation Version          :: Lates calcarifer Annotation Release
                                           100
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 7.2
            Annotation Method           :: Best-placed RefSeq; Gnomon
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..192
                     /organism="Lates calcarifer"
                     /isolate="ASB-BC8"
                     /db_xref="taxon:8187"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Brain, Kidney"
                     /dev_stage="adult"
                     /note="Back-crossed individual (decreased polymorphism)"
     Protein         1..192
                     /product="type-2 ice-structuring protein-like isoform X2"
                     /calculated_mol_wt=20587
     CDS             1..192
                     /gene="LOC108902415"
                     /coded_by="XM_018704260.1:95..673"
                     /db_xref="GeneID:108902415"
ORIGIN      
        1 mltvsllvca vmalasaddn sttsivdvsv aednsttsld vnaaeddstt skkeevapsc
       61 eigwsefngr cflfvstems wadaeknclh kkghlasvhn eeeykhiqav vnahtgghpt
      121 twvggsdcqk egiwlwsdgs gfefdswceg qpdnyvgaes clqinanesh cwndfpcstv
      181 lpsvcasvpq ss
//

LOCUS       XP_018559777             192 aa            linear   VRT 14-OCT-2016
DEFINITION  PREDICTED: type-2 ice-structuring protein-like isoform X3 [Lates
            calcarifer].
ACCESSION   XP_018559777
VERSION     XP_018559777.1
DBLINK      BioProject: PRJNA345597
DBSOURCE    REFSEQ: accession XM_018704261.1
KEYWORDS    RefSeq.
SOURCE      Lates calcarifer (barramundi perch)
  ORGANISM  Lates calcarifer
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Carangaria; Carangaria incertae sedis;
            Centropomidae; Lates.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_017366387.1) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI
            Annotation Status           :: Full annotation
            Annotation Version          :: Lates calcarifer Annotation Release
                                           100
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 7.2
            Annotation Method           :: Best-placed RefSeq; Gnomon
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..192
                     /organism="Lates calcarifer"
                     /isolate="ASB-BC8"
                     /db_xref="taxon:8187"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Brain, Kidney"
                     /dev_stage="adult"
                     /note="Back-crossed individual (decreased polymorphism)"
     Protein         1..192
                     /product="type-2 ice-structuring protein-like isoform X3"
                     /calculated_mol_wt=20586
     CDS             1..192
                     /gene="LOC108902415"
                     /coded_by="XM_018704261.1:95..673"
                     /db_xref="GeneID:108902415"
ORIGIN      
        1 mltvsllvca vmalasaddn sttsivdvsv aednsttsld vnaaednstt skkeevapsc
       61 eigwsefngr cflfvstems wadaeknclh kkghlasvhn eeeykhiqav vnahtgghpt
      121 twvggsdcqk egiwlwsdgs gfefdswceg qpdnyvgaes clqinanesh cwndfpcstv
      181 lpsvcasvpq ss
//

LOCUS       XP_018559779             191 aa            linear   VRT 14-OCT-2016
DEFINITION  PREDICTED: type-2 ice-structuring protein-like isoform X5 [Lates
            calcarifer].
ACCESSION   XP_018559779
VERSION     XP_018559779.1
DBLINK      BioProject: PRJNA345597
DBSOURCE    REFSEQ: accession XM_018704263.1
KEYWORDS    RefSeq.
SOURCE      Lates calcarifer (barramundi perch)
  ORGANISM  Lates calcarifer
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Carangaria; Carangaria incertae sedis;
            Centropomidae; Lates.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_017366387.1) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI
            Annotation Status           :: Full annotation
            Annotation Version          :: Lates calcarifer Annotation Release
                                           100
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 7.2
            Annotation Method           :: Best-placed RefSeq; Gnomon
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..191
                     /organism="Lates calcarifer"
                     /isolate="ASB-BC8"
                     /db_xref="taxon:8187"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Brain, Kidney"
                     /dev_stage="adult"
                     /note="Back-crossed individual (decreased polymorphism)"
     Protein         1..191
                     /product="type-2 ice-structuring protein-like isoform X5"
                     /calculated_mol_wt=20486
     CDS             1..191
                     /gene="LOC108902415"
                     /coded_by="XM_018704263.1:95..670"
                     /db_xref="GeneID:108902415"
ORIGIN      
        1 mltvsllvca vmalasaddn sttsldvnaa ednsttsldv naaeddstts kkeevapsce
       61 igwsefngrc flfvstemsw adaeknclhk kghlasvhne eeykhiqavv nahtgghptt
      121 wvggsdcqke giwlwsdgsg fefdswcegq pdnyvgaesc lqinaneshc wndfpcstvl
      181 psvcasvpqs s
//

LOCUS       XP_018559780             178 aa            linear   VRT 14-OCT-2016
DEFINITION  PREDICTED: type-2 ice-structuring protein-like isoform X6 [Lates
            calcarifer].
ACCESSION   XP_018559780
VERSION     XP_018559780.1
DBLINK      BioProject: PRJNA345597
DBSOURCE    REFSEQ: accession XM_018704264.1
KEYWORDS    RefSeq.
SOURCE      Lates calcarifer (barramundi perch)
  ORGANISM  Lates calcarifer
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Carangaria; Carangaria incertae sedis;
            Centropomidae; Lates.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_017366387.1) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI
            Annotation Status           :: Full annotation
            Annotation Version          :: Lates calcarifer Annotation Release
                                           100
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 7.2
            Annotation Method           :: Best-placed RefSeq; Gnomon
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..178
                     /organism="Lates calcarifer"
                     /isolate="ASB-BC8"
                     /db_xref="taxon:8187"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Brain, Kidney"
                     /dev_stage="adult"
                     /note="Back-crossed individual (decreased polymorphism)"
     Protein         1..178
                     /product="type-2 ice-structuring protein-like isoform X6"
                     /calculated_mol_wt=19168
     CDS             1..178
                     /gene="LOC108902415"
                     /coded_by="XM_018704264.1:95..631"
                     /db_xref="GeneID:108902415"
ORIGIN      
        1 mltvsllvca vmalasaddn sttsldvnaa eddsttskke evapsceigw sefngrcflf
       61 vstemswada eknclhkkgh lasvhneeey khiqavvnah tgghpttwvg gsdcqkegiw
      121 lwsdgsgfef dswcegqpdn yvgaesclqi naneshcwnd fpcstvlpsv casvpqss
//

LOCUS       XP_018559781             178 aa            linear   VRT 14-OCT-2016
DEFINITION  PREDICTED: type-2 ice-structuring protein-like isoform X7 [Lates
            calcarifer].
ACCESSION   XP_018559781
VERSION     XP_018559781.1
DBLINK      BioProject: PRJNA345597
DBSOURCE    REFSEQ: accession XM_018704265.1
KEYWORDS    RefSeq.
SOURCE      Lates calcarifer (barramundi perch)
  ORGANISM  Lates calcarifer
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Carangaria; Carangaria incertae sedis;
            Centropomidae; Lates.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_017366387.1) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI
            Annotation Status           :: Full annotation
            Annotation Version          :: Lates calcarifer Annotation Release
                                           100
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 7.2
            Annotation Method           :: Best-placed RefSeq; Gnomon
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..178
                     /organism="Lates calcarifer"
                     /isolate="ASB-BC8"
                     /db_xref="taxon:8187"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Brain, Kidney"
                     /dev_stage="adult"
                     /note="Back-crossed individual (decreased polymorphism)"
     Protein         1..178
                     /product="type-2 ice-structuring protein-like isoform X7"
                     /calculated_mol_wt=19167
     CDS             1..178
                     /gene="LOC108902415"
                     /coded_by="XM_018704265.1:95..631"
                     /db_xref="GeneID:108902415"
ORIGIN      
        1 mltvsllvca vmalasaddn sttsldvnaa ednsttskke evapsceigw sefngrcflf
       61 vstemswada eknclhkkgh lasvhneeey khiqavvnah tgghpttwvg gsdcqkegiw
      121 lwsdgsgfef dswcegqpdn yvgaesclqi naneshcwnd fpcstvlpsv casvpqss
//

LOCUS       XP_018559782             192 aa            linear   VRT 14-OCT-2016
DEFINITION  PREDICTED: type-2 ice-structuring protein-like isoform X1 [Lates
            calcarifer].
ACCESSION   XP_018559782
VERSION     XP_018559782.1
DBLINK      BioProject: PRJNA345597
DBSOURCE    REFSEQ: accession XM_018704266.1
KEYWORDS    RefSeq.
SOURCE      Lates calcarifer (barramundi perch)
  ORGANISM  Lates calcarifer
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Carangaria; Carangaria incertae sedis;
            Centropomidae; Lates.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_017366387.1) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI
            Annotation Status           :: Full annotation
            Annotation Version          :: Lates calcarifer Annotation Release
                                           100
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 7.2
            Annotation Method           :: Best-placed RefSeq; Gnomon
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..192
                     /organism="Lates calcarifer"
                     /isolate="ASB-BC8"
                     /db_xref="taxon:8187"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Brain, Kidney"
                     /dev_stage="adult"
                     /note="Back-crossed individual (decreased polymorphism)"
     Protein         1..192
                     /product="type-2 ice-structuring protein-like isoform X1"
                     /calculated_mol_wt=20564
     CDS             1..192
                     /gene="LOC108902416"
                     /coded_by="XM_018704266.1:92..670"
                     /db_xref="GeneID:108902416"
ORIGIN      
        1 mltvsllvca vmalasaddn sttslvdvsv aednsttsld vnaaeddstt skkeevapsc
       61 eigwsefngr cflfvstems wadaekncll kkghlasvhn eeeykhiqav vnahtgghpt
      121 twvggsdcqk egiwlwsdgs gfefdswceg epdnyvgaes clqinanesh cwndfpcstv
      181 lpsvcasvpq ss
//

LOCUS       XP_018559783             179 aa            linear   VRT 14-OCT-2016
DEFINITION  PREDICTED: type-2 ice-structuring protein-like isoform X2 [Lates
            calcarifer].
ACCESSION   XP_018559783
VERSION     XP_018559783.1
DBLINK      BioProject: PRJNA345597
DBSOURCE    REFSEQ: accession XM_018704267.1
KEYWORDS    RefSeq.
SOURCE      Lates calcarifer (barramundi perch)
  ORGANISM  Lates calcarifer
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Carangaria; Carangaria incertae sedis;
            Centropomidae; Lates.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_017366387.1) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI
            Annotation Status           :: Full annotation
            Annotation Version          :: Lates calcarifer Annotation Release
                                           100
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 7.2
            Annotation Method           :: Best-placed RefSeq; Gnomon
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..179
                     /organism="Lates calcarifer"
                     /isolate="ASB-BC8"
                     /db_xref="taxon:8187"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Brain, Kidney"
                     /dev_stage="adult"
                     /note="Back-crossed individual (decreased polymorphism)"
     Protein         1..179
                     /product="type-2 ice-structuring protein-like isoform X2"
                     /calculated_mol_wt=19245
     CDS             1..179
                     /gene="LOC108902416"
                     /coded_by="XM_018704267.1:91..630"
                     /db_xref="GeneID:108902416"
ORIGIN      
        1 mltvsllvca vmalasaddn sttslvdvsv aeddsttskk eevapsceig wsefngrcfl
       61 fvstemswad aekncllkkg hlasvhneee ykhiqavvna htgghpttwv ggsdcqkegi
      121 wlwsdgsgfe fdswcegepd nyvgaesclq inaneshcwn dfpcstvlps vcasvpqss
//

LOCUS       XP_018559784             178 aa            linear   VRT 14-OCT-2016
DEFINITION  PREDICTED: type-2 ice-structuring protein-like isoform X3 [Lates
            calcarifer].
ACCESSION   XP_018559784
VERSION     XP_018559784.1
DBLINK      BioProject: PRJNA345597
DBSOURCE    REFSEQ: accession XM_018704268.1
KEYWORDS    RefSeq.
SOURCE      Lates calcarifer (barramundi perch)
  ORGANISM  Lates calcarifer
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Carangaria; Carangaria incertae sedis;
            Centropomidae; Lates.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_017366387.1) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI
            Annotation Status           :: Full annotation
            Annotation Version          :: Lates calcarifer Annotation Release
                                           100
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 7.2
            Annotation Method           :: Best-placed RefSeq; Gnomon
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..178
                     /organism="Lates calcarifer"
                     /isolate="ASB-BC8"
                     /db_xref="taxon:8187"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Brain, Kidney"
                     /dev_stage="adult"
                     /note="Back-crossed individual (decreased polymorphism)"
     Protein         1..178
                     /product="type-2 ice-structuring protein-like isoform X3"
                     /calculated_mol_wt=19145
     CDS             1..178
                     /gene="LOC108902416"
                     /coded_by="XM_018704268.1:91..627"
                     /db_xref="GeneID:108902416"
ORIGIN      
        1 mltvsllvca vmalasaddn sttsldvnaa eddsttskke evapsceigw sefngrcflf
       61 vstemswada ekncllkkgh lasvhneeey khiqavvnah tgghpttwvg gsdcqkegiw
      121 lwsdgsgfef dswcegepdn yvgaesclqi naneshcwnd fpcstvlpsv casvpqss
//

LOCUS       XP_018559785             192 aa            linear   VRT 14-OCT-2016
DEFINITION  PREDICTED: type-2 ice-structuring protein-like [Lates calcarifer].
ACCESSION   XP_018559785
VERSION     XP_018559785.1
DBLINK      BioProject: PRJNA345597
DBSOURCE    REFSEQ: accession XM_018704269.1
KEYWORDS    RefSeq.
SOURCE      Lates calcarifer (barramundi perch)
  ORGANISM  Lates calcarifer
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Carangaria; Carangaria incertae sedis;
            Centropomidae; Lates.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_017366387.1) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI
            Annotation Status           :: Full annotation
            Annotation Version          :: Lates calcarifer Annotation Release
                                           100
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 7.2
            Annotation Method           :: Best-placed RefSeq; Gnomon
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..192
                     /organism="Lates calcarifer"
                     /isolate="ASB-BC8"
                     /db_xref="taxon:8187"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Brain, Kidney"
                     /dev_stage="adult"
                     /note="Back-crossed individual (decreased polymorphism)"
     Protein         1..192
                     /product="type-2 ice-structuring protein-like"
                     /calculated_mol_wt=20563
     CDS             1..192
                     /gene="LOC108902418"
                     /coded_by="XM_018704269.1:92..670"
                     /db_xref="GeneID:108902418"
ORIGIN      
        1 mltvsllvca vmalasaddn sttslvdvsv aednsttsld vnaaednstt skkeevapsc
       61 eigwsefngr cflfvstems wadaekncll kkghlasvhn eeeykhiqav vnahtgghpt
      121 twvggsdcqk egiwlwsdgs gfefdswceg epdnyvgaes clqinanesh cwndfpcstv
      181 lpsvcasvpq ss
//

LOCUS       XP_018559802             179 aa            linear   VRT 14-OCT-2016
DEFINITION  PREDICTED: type-2 ice-structuring protein-like [Lates calcarifer].
ACCESSION   XP_018559802
VERSION     XP_018559802.1
DBLINK      BioProject: PRJNA345597
DBSOURCE    REFSEQ: accession XM_018704286.1
KEYWORDS    RefSeq.
SOURCE      Lates calcarifer (barramundi perch)
  ORGANISM  Lates calcarifer
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Carangaria; Carangaria incertae sedis;
            Centropomidae; Lates.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_017366388.1) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI
            Annotation Status           :: Full annotation
            Annotation Version          :: Lates calcarifer Annotation Release
                                           100
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 7.2
            Annotation Method           :: Best-placed RefSeq; Gnomon
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..179
                     /organism="Lates calcarifer"
                     /isolate="ASB-BC8"
                     /db_xref="taxon:8187"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Brain, Kidney"
                     /dev_stage="adult"
                     /note="Back-crossed individual (decreased polymorphism)"
     Protein         1..179
                     /product="type-2 ice-structuring protein-like"
                     /calculated_mol_wt=19201
     CDS             1..179
                     /gene="LOC108902432"
                     /coded_by="XM_018704286.1:95..634"
                     /db_xref="GeneID:108902432"
ORIGIN      
        1 mltvsllvca mmalasaddn sttslvdvsv aeddsttslv ekvapsceig wsefngrcfl
       61 fvstemswad aekncllkkg hlasvhneee ykhiqavvna htgghpatwv ggsdcqkegi
      121 wlwsdgssfe fnswcegepd nfagaesclq inaneshcwn dflcsvvlps vcasvpqss
//

LOCUS       XP_026209209             160 aa            linear   VRT 13-APR-2020
DEFINITION  type-2 ice-structuring protein-like [Anabas testudineus].
ACCESSION   XP_026209209
VERSION     XP_026209209.1
DBLINK      BioProject: PRJNA489257
DBSOURCE    REFSEQ: accession XM_026353424.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Anabas testudineus (climbing perch)
  ORGANISM  Anabas testudineus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Anabantaria; Anabantiformes; Anabantoidei;
            Anabantidae; Anabas.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_046627.1) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI
            Annotation Status           :: Full annotation
            Annotation Name             :: Anabas testudineus Annotation
                                           Release 101
            Annotation Version          :: 101
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 8.4
            Annotation Method           :: Best-placed RefSeq; Gnomon
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..160
                     /organism="Anabas testudineus"
                     /db_xref="taxon:64144"
                     /chromosome="18"
                     /sex="male"
     Protein         1..160
                     /product="type-2 ice-structuring protein-like"
                     /calculated_mol_wt=17772
     CDS             1..160
                     /gene="LOC113157802"
                     /coded_by="XM_026353424.1:1..483"
                     /db_xref="GeneID:113157802"
ORIGIN      
        1 mkmltvsllv cammaltraa dqshaikraa scpsgwtgyd grcflyvptq mtwadaeknc
       61 lyhggnlasv hsfdehhviq smilklthsy pltwiggsda qqegtwfwsd gtafrlqywa
      121 pgqpdnmasa hcllmnfgdl kkfddqpcsy rkasvcakkq
//

LOCUS       ISP2_BRASG               168 aa            linear   VRT 18-SEP-2019
DEFINITION  RecName: Full=Type-2 ice-structuring protein; AltName: Full=Type II
            antifreeze protein; AltName: Full=lpAFP; Flags: Precursor.
ACCESSION   A0ZT93
VERSION     A0ZT93.1
DBSOURCE    UniProtKB: locus ISP2_BRASG, accession A0ZT93;
            class: standard.
            created: Sep 12, 2018.
            sequence updated: Jan 23, 2007.
            annotation updated: Sep 18, 2019.
            xrefs: AB283044.1, BAF37106.1, 2ZIB_A
            xrefs (non-sequence databases): PDBsum:2ZIB, SMR:A0ZT93,
            EvolutionaryTrace:A0ZT93, GO:0005576, GO:0030246,
            Gene3D:3.10.100.10, InterPro:IPR002353, InterPro:IPR001304,
            InterPro:IPR016186, InterPro:IPR018378, InterPro:IPR016187,
            Pfam:PF00059, PRINTS:PR00356, SMART:SM00034, SUPFAM:SSF56436,
            PROSITE:PS00615, PROSITE:PS50041
KEYWORDS    3D-structure; Antifreeze protein; Disulfide bond; Lectin; Secreted;
            Signal.
SOURCE      Brachyopsis segaliensis
  ORGANISM  Brachyopsis segaliensis
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Perciformes; Cottioidei; Cottales;
            Agonidae; Brachyopsinae; Brachyopsis.
REFERENCE   1  (residues 1 to 168)
  AUTHORS   Nishimiya,Y., Kondo,H., Yasui,M., Sugimoto,H., Noro,N., Sato,R.,
            Suzuki,M., Miura,A. and Tsuda,S.
  TITLE     Crystallization and preliminary X-ray crystallographic analysis of
            Ca2+-independent and Ca2+-dependent species of the type II
            antifreeze protein
  JOURNAL   Acta Crystallogr. F Struct. Biol. Commun. 62, 538-541 (2006)
   PUBMED   16754975
  REMARK    NUCLEOTIDE SEQUENCE [MRNA], CRYSTALLIZATION, AND SUBCELLULAR
            LOCATION.
REFERENCE   2  (residues 1 to 168)
  AUTHORS   Nishimiya,Y., Kondo,H., Takamichi,M., Sugimoto,H., Suzuki,M.,
            Miura,A. and Tsuda,S.
  TITLE     Crystal structure and mutational analysis of Ca2+-independent type
            II antifreeze protein from longsnout poacher, Brachyopsis rostratus
  JOURNAL   J. Mol. Biol. 382 (3), 734-746 (2008)
   PUBMED   18674542
  REMARK    X-RAY CRYSTALLOGRAPHY (1.34 ANGSTROMS) OF 42-168, FUNCTION,
            DISULFIDE BONDS, AND MUTAGENESIS OF ILE-99; ALA-112 AND LEU-153.
COMMENT     [FUNCTION] Has antifreeze activity to protect fish blood from
            freezing at subzero sea water temperatures (Probable). Binds to ice
            crystals and inhibits their growth. The thermal hysteresis (TH)
            activity, the ability to lower the blood freezing point, is
            approximately 0.45 degrees Celsius at 0.15 mM for this protein
            (PubMed:18674542). {ECO:0000269|PubMed:18674542,
            ECO:0000305|PubMed:18674542}.
            [SUBCELLULAR LOCATION] Secreted {ECO:0000269|PubMed:16754975}.
FEATURES             Location/Qualifiers
     source          1..168
                     /organism="Brachyopsis segaliensis"
                     /db_xref="taxon:1633465"
     Protein         1..168
                     /product="Type-2 ice-structuring protein"
                     /note="Type II antifreeze protein; lpAFP"
                     /UniProtKB_evidence="Evidence at protein level"
     Region          1..17
                     /region_name="Signal"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="{ECO:0000255}."
     Region          18..34
                     /region_name="Propeptide"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="{ECO:0000250|UniProtKB:P05140}.
                     /FTId=PRO_0000445011."
     Region          35..168
                     /region_name="Mature chain"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Type-2 ice-structuring protein.
                     {ECO:0000250|UniProtKB:P05140}. /FTId=PRO_5002631706."
     Region          45..163
                     /region_name="CLECT"
                     /note="C-type lectin (CTL) or carbohydrate-recognition
                     domain (CRD); smart00034"
                     /db_xref="CDD:214480"
     Bond            bond(45,56)
                     /bond_type="disulfide"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="{ECO:0000244|PDB:2ZIB,
                     ECO:0000269|PubMed:18674542}."
     Region          50..52
                     /region_name="Beta-strand region"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="{ECO:0000244|PDB:2ZIB}."
     Region          52..164
                     /region_name="Domain"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="C-type lectin.
                     {ECO:0000255|PROSITE-ProRule:PRU00040}."
     Region          55..64
                     /region_name="Beta-strand region"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="{ECO:0000244|PDB:2ZIB}."
     Region          66..75
                     /region_name="Helical region"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="{ECO:0000244|PDB:2ZIB}."
     Bond            bond(73,163)
                     /bond_type="disulfide"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="{ECO:0000244|PDB:2ZIB,
                     ECO:0000255|PROSITE-ProRule:PRU00040,
                     ECO:0000269|PubMed:18674542}."
     Region          78..80
                     /region_name="Beta-strand region"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="{ECO:0000244|PDB:2ZIB}."
     Region          86..95
                     /region_name="Helical region"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="{ECO:0000244|PDB:2ZIB}."
     Site            99
                     /site_type="mutagenized"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="I->F: Has 60% thermal hysteresis (TH) activity of
                     that of the wild-type. Has 60% thermal hysteresis (TH)
                     activity of that of the wild-type; when associated with
                     F-112. {ECO:0000269|PubMed:18674542}."
     Region          100..105
                     /region_name="Beta-strand region"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="{ECO:0000244|PDB:2ZIB}."
     Bond            bond(107,138)
                     /bond_type="disulfide"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="{ECO:0000244|PDB:2ZIB,
                     ECO:0000269|PubMed:18674542}."
     Site            112
                     /site_type="mutagenized"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="A->F: No effect on thermal hysteresis (TH)
                     activity. Has 60% thermal hysteresis (TH) activity of that
                     of the wild-type; when associated with F-99.
                     {ECO:0000269|PubMed:18674542}."
     Bond            bond(127,149)
                     /bond_type="disulfide"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="{ECO:0000244|PDB:2ZIB,
                     ECO:0000269|PubMed:18674542}."
     Site            order(137,141,143,149,151..154,157..159)
                     /site_type="other"
                     /note="ligand binding surface [chemical binding]"
                     /db_xref="CDD:153057"
     Bond            bond(139,155)
                     /bond_type="disulfide"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="{ECO:0000244|PDB:2ZIB,
                     ECO:0000255|PROSITE-ProRule:PRU00040,
                     ECO:0000269|PubMed:18674542}."
     Region          139..142
                     /region_name="Beta-strand region"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="{ECO:0000244|PDB:2ZIB}."
     Region          150..153
                     /region_name="Beta-strand region"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="{ECO:0000244|PDB:2ZIB}."
     Site            153
                     /site_type="mutagenized"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="L->F: Nearly identical thermal hysteresis (TH)
                     activity with that of the wild-type.
                     {ECO:0000269|PubMed:18674542}."
     Region          159..166
                     /region_name="Beta-strand region"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="{ECO:0000244|PDB:2ZIB}."
ORIGIN      
        1 mltvsllvca mmaltqadhd gvlkgtatea gevspvfrsr ralvcpagwt lhgqrcfyse
       61 atamtwdlae ancvnkgghl asihsleeql yikdivagiv wiggsackva gawswtdgtp
      121 vdyrtwcptk pndilsdccm qmtaavdkcw ddlpcpasha sicakaai
//

LOCUS       XP_028420054             160 aa            linear   VRT 01-APR-2019
DEFINITION  type-2 ice-structuring protein-like [Perca flavescens].
ACCESSION   XP_028420054
VERSION     XP_028420054.1
DBLINK      BioProject: PRJNA529871
DBSOURCE    REFSEQ: accession XM_028564253.1
KEYWORDS    RefSeq.
SOURCE      Perca flavescens (yellow perch)
  ORGANISM  Perca flavescens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Perciformes; Percoidei; Percidae;
            Percinae; Perca.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_041349.1) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI
            Annotation Status           :: Full annotation
            Annotation Name             :: Perca flavescens Annotation Release
                                           100
            Annotation Version          :: 100
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 8.2
            Annotation Method           :: Best-placed RefSeq; Gnomon
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..160
                     /organism="Perca flavescens"
                     /isolate="YP-PL-M2"
                     /db_xref="taxon:8167"
                     /chromosome="19"
                     /sex="male"
                     /tissue_type="blood"
                     /dev_stage="Adult"
                     /country="USA: Wisconsin, Plum Lake"
                     /collection_date="28-Apr-2017"
                     /collected_by="Wes Larson, Wisconsin Cooperative Fishery
                     Research Unit, University of Wisconsin-Stevens Point, 800
                     Reserve Street Stevens Point, WI 54481"
     Protein         1..160
                     /product="type-2 ice-structuring protein-like"
                     /calculated_mol_wt=17895
     Region          32..156
                     /region_name="CLECT"
                     /note="C-type lectin (CTL) or carbohydrate-recognition
                     domain (CRD); smart00034"
                     /db_xref="CDD:214480"
     Site            order(130,134,136,142,144..147,150..152)
                     /site_type="other"
                     /note="ligand binding surface [chemical binding]"
                     /db_xref="CDD:153057"
     CDS             1..160
                     /gene="LOC114545755"
                     /coded_by="XM_028564253.1:16..498"
                     /db_xref="GeneID:114545755"
ORIGIN      
        1 mkvlivsllv cammaltraa gkshivkrss scpsgwtgyn grcflyvpta mtwadaeknc
       61 lyhggnlasv hsfeehhviq smilrithmy pltwlggsda qqegswfwsd gtpfrfnfws
      121 pgqpdnrgna hcllmnfgdq nkfddqpcsf rmpsvcakrq
//

LOCUS       XP_030285706             211 aa            linear   VRT 18-AUG-2019
DEFINITION  galactose-specific lectin nattectin-like isoform X2 [Sparus
            aurata].
ACCESSION   XP_030285706
VERSION     XP_030285706.1
DBLINK      BioProject: PRJNA558036
DBSOURCE    REFSEQ: accession XM_030429846.1
KEYWORDS    RefSeq.
SOURCE      Sparus aurata (gilthead seabream)
  ORGANISM  Sparus aurata
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Eupercaria; Spariformes; Sparidae; Sparus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_044196.1) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI
            Annotation Status           :: Full annotation
            Annotation Name             :: Sparus aurata Annotation Release 100
            Annotation Version          :: 100
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 8.2
            Annotation Method           :: Best-placed RefSeq; Gnomon
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..211
                     /organism="Sparus aurata"
                     /db_xref="taxon:8175"
                     /chromosome="10"
     Protein         1..211
                     /product="galactose-specific lectin nattectin-like isoform
                     X2"
                     /calculated_mol_wt=23726
     CDS             1..211
                     /gene="LOC115589122"
                     /coded_by="XM_030429846.1:137..772"
                     /db_xref="GeneID:115589122"
ORIGIN      
        1 mnyisncmke lvfihrgrrq dnppqvitln icitsttkml tvsllvcaim altgaaavpe
       61 getdlnagpe gnsntaevvp scpggwtgyn drcflyipsl mswanaekhc qtqggnlasv
      121 hsfdeqraiq tmiqrqtfmy prtwlggyda mqegtwfwsd gtpfhftywa pgqpdnflfa
      181 qhclsmnygd erkfddhrcd frepfvcakk l
//

LOCUS       XP_030609865             170 aa            linear   VRT 06-SEP-2019
DEFINITION  type-2 ice-structuring protein-like [Archocentrus centrarchus].
ACCESSION   XP_030609865
VERSION     XP_030609865.1
DBLINK      BioProject: PRJNA559550
DBSOURCE    REFSEQ: accession XM_030754005.1
KEYWORDS    RefSeq.
SOURCE      Archocentrus centrarchus (flier cichlid)
  ORGANISM  Archocentrus centrarchus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Ovalentaria; Cichlomorphae; Cichliformes;
            Cichlidae; New World cichlids; Cichlasomatinae; Heroini;
            Archocentrus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_044363.1) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI
            Annotation Status           :: Full annotation
            Annotation Name             :: Archocentrus centrarchus Annotation
                                           Release 100
            Annotation Version          :: 100
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 8.2
            Annotation Method           :: Best-placed RefSeq; Gnomon
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..170
                     /organism="Archocentrus centrarchus"
                     /isolate="MPI-CPG fArcCen1"
                     /db_xref="taxon:63155"
                     /chromosome="18"
                     /sex="male"
                     /tissue_type="muscle"
                     /country="Nicaragua"
                     /lat_lon="12.8654 N 85.2072 W"
                     /collection_date="2016-03-09"
                     /collected_by="Axel Meyer, Andreas Kautt, Paolo Franchini"
     Protein         1..170
                     /product="type-2 ice-structuring protein-like"
                     /calculated_mol_wt=18972
     CDS             1..170
                     /gene="LOC115797424"
                     /coded_by="XM_030754005.1:46..558"
                     /db_xref="GeneID:115797424"
ORIGIN      
        1 mkllsvsahl calmaltfav egdsdaegpv ttvkshsgcp rgwiefnnrc fhyvttrmtw
       61 anaeknclll ggnlasvhnd meyfeiqklt athgyrqawi ggtdavqkkv wfwsdgtpfh
      121 ysnwcpgess ngrndhclrm nysgakcwdd vrcairlpsv cainmeclqe
//

