LOCUS       KPLACBG      1565 bp    DNA             BCT       12-SEP-1993
DEFINITION  Klebsiella pneumonia beta-lactamase gene.
ACCESSION   X04515
VERSION     X04515.1  GI:43812
KEYWORDS    beta-lactamase; inverted repeat; lactamase.
SOURCE      Klebsiella pneumoniae.
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Proteobacteria; gamma subdivision; Enterobacteriaceae;
            Klebsiella.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Arakawa,Y., Ohta,M., Kido,N., Fujii,Y., Komatsu,T. and Kato,N.
  TITLE     Close evolutionary relationship between the chromosomally encoded
            beta-lactamase gene of Klebsiella pneumoniae and the TEM
            beta-lactamase gene mediated by R plasmids
  JOURNAL   FEBS Lett. 207 (1), 69-74 (1986)
  MEDLINE   87030906
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Klebsiella pneumoniae"
                     /strain="LEN-1"
                     /db_xref="taxon:573"
     promoter        199..204
                     /note="pot. -35 region"
     promoter        223..228
                     /note="pot. -10 region"
     CDS             286..1125
                     /note="put. pre-beta-lactamase (aa 1-279)"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="CAA28198.1"
                     /db_xref="GI:43813"
                     /db_xref="SWISS-PROT:P05192"
                     /translation="MRYVRLCVISLLATLPLVVYAGPQPLEQIKQSESQLSGRVGMVE
                     MDLANGRTLAAWRADERFPMVSTFKVLLCGAVLARVDAGLEQLDRRIHYRQQDLVDYS
                     PVSEKHLVDGMTIGELCAAAITLSDNSAGNLLLATVGGPAGLTAFLRQIGDNVTRLDR
                     WETALNEALPGDARDTTTPASMAATLRKLLTAQHLSARSQQQLLQWMVDDRVAGPLIR
                     AVLPPGWFIADKTGAGERGARGIVALLGPDGKPERIVVIYLRDTPASMAERNQHIAGI
                     GQR"
     repeat_unit     1140..1146
                     /note="inverted repeat A"
     terminator      1140..1168
                     /note="region of dyad symmetry (pot. terminator)"
     repeat_unit     1151..1154
                     /note="inverted repeat B"
     repeat_unit     1155..1158
                     /note="inverted repeat B'"
     repeat_unit     1162..1168
                     /note="inverted repeat A'"
BASE COUNT      318 a    461 c    478 g    308 t
ORIGIN
        1 cccgggatgg cgtatatcat tggcgaacgg cggagtccgg cgggcattgt cgtcaacaaa
       61 cggcgccacg tgagtgcggc attacgtact ttcttatagt tcatcgcggc cttgagtcaa
      121 aaaatagcgt gctgaggcag ggctaaatat tgattattcg aaataaaaga tgacaaatga
      181 tgaaggaaaa aagaggaatt gtgaatcagc aaaacgccgg gttattctta tttgtcgccc
      241 ctttgctcgc ccttatcggc cctcactcaa ggaagtattg cggttatgcg ttatgttcgc
      301 ctgtgtgtta tctccctgtt agccaccctg ccactggtgg tatacgccgg tccacagccg
      361 cttgagcaga ttaaacaaag cgaaagccag ctgtcgggcc gcgtggggat ggtggaaatg
      421 gatctggcca acggccgcac gctggccgcc tggcgcgccg atgaacgctt tcccatggtg
      481 agcaccttta aagtgctgct gtgcggcgcg gtgctggcgc gggtggatgc cgggctcgaa
      541 caactggatc ggcggatcca ctaccgccag caggatctgg tggactactc cccggtcagc
      601 gaaaaacacc ttgtcgacgg gatgacgatc ggcgaactct gcgccgccgc catcaccctg
      661 agcgataaca gcgctggcaa tctgctgctg gccaccgtcg gcggccccgc gggattaact
      721 gcctttctgc gccagatcgg tgacaacgtc acccgtcttg accgctggga aacggcactg
      781 aatgaggcgc ttcccggcga cgcgcgcgac accaccaccc cggccagcat ggccgccacg
      841 ctgcgcaaac tactgaccgc gcagcatctg agcgcccgtt cgcaacagca actcctgcag
      901 tggatggtgg acgatcgggt tgccggcccg ctgatccgcg ccgtgctgcc gccgggctgg
      961 tttatcgccg acaaaaccgg ggctggcgaa cggggtgcgc gcggcattgt cgccctgctc
     1021 ggcccggacg gcaaaccgga gcgcattgtg gtgatctatc tgcgggatac cccggcgagt
     1081 atggccgagc gtaatcaaca tatcgccggg atcggccagc gctgatcgag cactggcaac
     1141 gctaacccgg cggtaccgtg cgttagcgcg gcccgcagca cctggcaggc gtgccgcgcg
     1201 atatgactgg cggcggcatc ggacagatcc atcggtaata atagttgtaa ccgggtcaag
     1261 ggtaacgcca taaaggtcgc ctcctgattg tatttcgaac tgtcgcacag caggatgctt
     1321 ctcgcgctga cctggctgac ggtctccttg acggtaacct tgttctcatc aggggtgaaa
     1381 atagctgtcc cagcgctggc ggagataaag gccgtatcga tagccaggtg gagtaacgtt
     1441 cgcgccgccg attcgcccac gcaggagcga ttctccgcac acgtgccgcc ggtgtggatc
     1501 acgccgcact ggctggcatc gatcagcagt tgggttatct caaaatcatt ggtcaccacc
     1561 tgcag
//
