LOCUS       AF112443      506 bp    mRNA            PLN       10-DEC-1999
DEFINITION  Capsicum annuum thionin-like protein (PepThi) mRNA, complete cds.
ACCESSION   AF112443
VERSION     AF112443.1  GI:6552501
KEYWORDS    .
SOURCE      Capsicum annuum.
  ORGANISM  Capsicum annuum
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            euphyllophytes; Spermatophyta; Magnoliophyta; eudicotyledons; core
            eudicots; Asteridae; euasterids I; Solanales; Solanaceae; Capsicum.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Oh,B.J., Ko,M.K., Kostenyuk,I., Shin,B. and Kim,K.S.
  TITLE     Coexpression of a defensin gene and a thionin-like gene via
            different signal transduction pathways in pepper and Colletotrichum
            gloeosporioides interactions
  JOURNAL   Plant Mol. Biol. 41, 313-319 (1999)
REFERENCE   2  (bases 1 to 506)
  AUTHORS   Oh,B.J., Ko,M.K., Kostenyuk,I.A., Shin,B.C. and Kim,K.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-1998) Kumho Life and Environmental Science
            Laboratory, Kumho Petrochemical Co., LTD., 1 Oryong-dong, Puk-gu,
            Kwangju 500-712, Korea
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Capsicum annuum"
                     /db_xref="taxon:4072"
     gene            1..506
                     /gene="PepThi"
     CDS             10..264
                     /gene="PepThi"
                     /codon_start=1
                     /product="thionin-like protein"
                     /protein_id="AAF16413.1"
                     /db_xref="GI:6552502"
                     /translation="MARSIYFMAFLVLAMTLFVAYGVQGKEICCKELTKPVKCSSDPL
                     CQKLCMEKEKYEDGHCFTILSKCLCMKRCNAKTLATELLA"
BASE COUNT      173 a     75 c     96 g    162 t
ORIGIN
        1 aaagagacta tggctcgttc catttacttc atggcatttc ttgtcttggc aatgaccctc
       61 tttgttgctt atggggtgca aggcaaggaa atttgctgta aagagctcac aaaacctgtt
      121 aaatgttcta gtgaccctct atgtcaaaaa ctctgtatgg agaaggagaa atatgaagat
      181 ggtcattgtt tcacaatcct aagcaagtgc ttatgcatga agagatgtaa tgctaaaact
      241 ctcgcaacag aattgcttgc ttgaagaaga gttcctcaag cagtaattaa gtttgattat
      301 ggatttagtg tcacacaaaa ttaaataaag tgttgccttt cttaaaaggg taacttataa
      361 tgttgtattc ttggtgtata gtagccattt gacacattaa attaaagttg tgacacatca
      421 atccttcatg tatcttctat taagtttgtg tgttttaatg aaaagagatc gtttacggtc
      481 taaaaaaaaa aaaaaaaaaa aaaaaa
//
LOCUS       AF128239      559 bp    mRNA            PLN       06-JUL-1999
DEFINITION  Capsicum chinense putative gamma-thionin precursor, mRNA, complete
            cds.
ACCESSION   AF128239
VERSION     AF128239.1  GI:4457222
KEYWORDS    .
SOURCE      Capsicum chinense.
  ORGANISM  Capsicum chinense
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            euphyllophytes; Spermatophyta; Magnoliophyta; eudicotyledons; core
            eudicots; Asteridae; euasterids I; Solanales; Solanaceae; Capsicum.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Aluru,M., Curry,J. and O'Connell,M.A.
  TITLE     Nucleotide sequence of a defensin or gamma-thionin-like gene
            (Accession No. AF128239) from habanero chile (PGR 99-070)
  JOURNAL   Plant Physiol. 120 (2), 633 (1999)
REFERENCE   2  (bases 1 to 559)
  AUTHORS   Curry,J., Aluru,M. and O'Connell,M.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-FEB-1999) Agronomy and Horticulture, New Mexico State
            University, P.O. Box 30003, MSC 3Q, Las Cruces, NM 88003, USA
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Capsicum chinense"
                     /cultivar="habanero"
                     /db_xref="taxon:80379"
     CDS             39..362
                     /note="defensin"
                     /codon_start=1
                     /product="putative gamma-thionin precursor"
                     /protein_id="AAD21200.1"
                     /db_xref="GI:4457223"
                     /translation="MARSIYFMAFLVLAVTLFVANGVQGQNNICKTTSKHFKGLCFAD
                     SKCRKVCIQEDKFEDGHCSKLQRKCLCTKNCVFDNIPNDVGTILVQDAKTLEAQLLEE
                     EILGL"
BASE COUNT      181 a     84 c    104 g    190 t
ORIGIN
        1 aaaaatattt gcaatctttt ttattaatca aagagactat ggctcgttcc atttacttca
       61 tggcatttct tgtcttggca gtgacactct ttgttgctaa tggggtgcaa ggtcaaaata
      121 acatttgcaa aacaacaagc aaacatttta aaggattatg ttttgccgac tctaaatgta
      181 gaaaagtttg tatccaggag gataaatttg aagatggtca ttgtagcaaa ctccaaagga
      241 agtgcctatg cactaagaat tgtgtatttg acaatattcc taatgatgtt ggaactattt
      301 tggttcagga tgctaaaact ctcgaagcac aattgcttga agaagagatt ctcgggctgt
      361 aattaagtct gattatggat ttagtgtcac acaaaattaa ataatgtgtt gcctttctta
      421 aaagagtaac ttacaatact gtgttcttgg cctatagtag ccatttgaca cattaaataa
      481 gttgtgacac ataaatccta tatgtatctt gtataaagtt ttatgtgttt taatgaaaag
      541 agaccgtcta ctgtccgta
//
LOCUS       BOAJ5280      351 bp    mRNA            PLN       05-AUG-1998
DEFINITION  Brassica oleracea mRNA for pollen coat protein gene class-'A' no.1
            (PCP-A1).
ACCESSION   AJ005280
VERSION     AJ005280.1  GI:3395459
KEYWORDS    pollen coat protein.
SOURCE      Brassica oleracea.
  ORGANISM  Brassica oleracea
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            euphyllophytes; Spermatophyta; Magnoliophyta; eudicotyledons;
            Rosidae; Capparales; Brassicaceae; Brassica.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Doughty,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-APR-1998) Doughty J., Department of Plant Sciences,
            University of Oxford, South Parks Road, Oxford, OX1 3RB, UK
REFERENCE   2  (bases 1 to 351)
  AUTHORS   Doughty,J., Dixon,S., Hiscock,S.J., Willis,A.C., Parkin,I.A.P. and
            Dickinson,H.G.
  TITLE     PCP-A1, a defensin-like brassica pollen coat protein that binds the
            S locus glycoprotein, is the product of gametophytic gene
            expression
  JOURNAL   Plant Cell 10 (1998) In press
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Brassica oleracea"
                     /cultivar="alboglabra"
                     /db_xref="taxon:3712"
                     /chromosome="18"
                     /tissue_type="anther"
                     /cell_type="pollen"
                     /dev_stage="anthers/trinucleate microspore stage"
                     /map="just below pN23b"
                     /note="B.rapa: chromosome 9"
     gene            31..348
                     /gene="PCP-A1"
     CDS             31..276
                     /gene="PCP-A1"
                     /function="putative cofactor in pollen recognition"
                     /codon_start=1
                     /evidence=experimental
                     /product="pollen coat protein, class 'A', 1 (PCP-A1)"
                     /protein_id="CAA06464.1"
                     /db_xref="GI:3395460"
                     /db_xref="SPTREMBL:O82789"
                     /translation="MKNTVKLSLIGFVMLTVLLLGETVIAQKRKPCYSQEPDKTCEVN
                     RCKANCVKKHKKILAFTSCIKENNGNMYCRCQYPCPP"
     sig_peptide     31..108
                     /gene="PCP-A1"
     mat_peptide     109..273
                     /gene="PCP-A1"
                     /product="pollen coat protein, class 'A', 1 (PCP-A1)"
     polyA_signal    343..348
                     /gene="PCP-A1"
BASE COUNT      136 a     65 c     47 g    103 t
ORIGIN
        1 ctttaaaaca caatcatttc attagctaaa atgaaaaaca cggttaaact ttcgcttatt
       61 ggttttgtca tgctcacagt tcttctatta ggagaaacgg tgattgctca gaaaagaaaa
      121 ccatgttatt cacaagagcc agacaaaaca tgtgaagtca atcgctgcaa agccaactgt
      181 gtcaaaaaac ataagaaaat actggctttt acctcgtgca ttaaagaaaa taacggaaat
      241 atgtattgcc gatgtcaata tccttgccct ccctaatttt ttttatctac accaaaactc
      301 aaataatatt tataataata ctatgacttt atcaaagaaa tgaataaaac a
//
LOCUS       BOAJ5281      540 bp    DNA             PLN       05-AUG-1998
DEFINITION  Brassica oleracea pollen coat protein (PCP-A1) gene.
ACCESSION   AJ005281
VERSION     AJ005281.1  GI:3395461
KEYWORDS    pollen coat protein.
SOURCE      Brassica oleracea.
  ORGANISM  Brassica oleracea
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            euphyllophytes; Spermatophyta; Magnoliophyta; eudicotyledons;
            Rosidae; Capparales; Brassicaceae; Brassica.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Doughty,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-APR-1998) Doughty J., Department of Plant Sciences,
            University of Oxford, South Parks Road, Oxford, OX1 3RB, UK
REFERENCE   2  (bases 1 to 540)
  AUTHORS   Doughty,J., Dixon,S., Hiscock,S.J., Willis,A.C., Parkin,I.A.P. and
            Dickinson,H.G.
  TITLE     PCP-A1, a defensin-like brassica pollen coat protein that binds the
            S locus glycoprotein, is the product of gametophytic gene
            expression
  JOURNAL   Plant Cell 10 (1998) In press
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Brassica oleracea"
                     /cultivar="alboglabra"
                     /db_xref="taxon:3712"
                     /chromosome="18"
                     /map="just below pN23b marker"
                     /note="B.rapa: chromosome 9"
     mRNA            join(<1..61,359..>540)
                     /gene="PCP-A1"
                     /evidence=experimental
     gene            1..540
                     /gene="PCP-A1"
     exon            <1..61
                     /gene="PCP-A1"
                     /number=1
     sig_peptide     join(1..61,359..375)
                     /gene="PCP-A1"
     CDS             join(1..61,359..>540)
                     /gene="PCP-A1"
                     /function="putative cofactor in S-specific pollen
                     recognition"
                     /codon_start=1
                     /evidence=experimental
                     /product="pollen coat protein class 'A', no. 1"
                     /protein_id="CAA06465.1"
                     /db_xref="GI:3395462"
                     /db_xref="SPTREMBL:O82789"
                     /translation="MKNTVKLSLIGFVMLTVLLLGETVIAQKRKPCYSQEPDKTCEVN
                     RCKANCVKKHKKILAFTSCIKENNGNMYCRCQYPCPP"
     intron          62..358
                     /gene="PCP-A1"
                     /number=1
     exon            359..>540
                     /gene="PCP-A1"
                     /number=2
     mat_peptide     376..>540
                     /gene="PCP-A1"
                     /product="pollen coat protein class 'A', no. 1"
BASE COUNT      204 a     73 c     72 g    191 t
ORIGIN
        1 atgaaaaaca cggttaaact ttcgcttatt ggttttgtca tgctcacagt tcttctatta
       61 ggtttgtatt tacatttaca acttcatgaa tttattatat aatcattcca aaatagattt
      121 tacttgatga tgtatgtatt tatataccta tataaattga atgtctaatt atataatatt
      181 gtgtttaatg atttttcaag aataataaat gatttagaaa gaagaacttt aaacatatag
      241 ttcttttttt ataagtatgc agaaaactct ctaaaaatat gtttgtttta caaaaaaaaa
      301 tatcagtttt tattttaaat atgaaactaa agttacgttt taattattat aataacagga
      361 gaaacggtga ttgctcagaa aagaaaacca tgttattcac aagagccaga caaaacatgt
      421 gaagtcaatc gctgcaaagc caactgtgtc aaaaaacata agaaaatact ggcttttacc
      481 tcgtgcatta aagaaaataa cggaaatatg tattgccgat gtcaatatcc ttgccctccc
//
LOCUS       CAGT         1390 bp    DNA             PLN       20-MAR-1998
DEFINITION  C.annuum gamma thionin gene.
ACCESSION   X95363
VERSION     X95363.1  GI:1171502
KEYWORDS    gamma-thionin.
SOURCE      Capsicum annuum.
  ORGANISM  Capsicum annuum
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            euphyllophytes; Spermatophyta; Magnoliophyta; eudicotyledons;
            Asteridae; Solananae; Solanales; Solanaceae; Capsicum.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Meyer,B., Houlne,G., Pozueta-Romero,J., Schantz,M.L. and Schantz,R.
  TITLE     Fruit-specific expression of a defensin-type gene family in bell
            pepper. Upregulation during ripening and upon wounding
  JOURNAL   Plant Physiol. 112 (2), 615-622 (1996)
  MEDLINE   97037730
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Meyer,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-1996) B. Meyer, Institut de Biologie Moleculaire
            des, Plantes, 2, rue du Gl Zimmer, F-67000 STRASBOURG, FRANCE
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Capsicum annuum"
                     /cultivar="Yolo wonder"
                     /db_xref="taxon:4072"
                     /tissue_type="fruit"
                     /clone_lib="lambda gem 11"
                     /clone="j1-1"
     exon            <105..168
                     /number=1
     CDS             join(105..168,1017..1180)
                     /codon_start=1
                     /product="gamma-thionin"
                     /protein_id="CAA64653.1"
                     /db_xref="GI:1171503"
                     /db_xref="SPTREMBL:Q43413"
                     /translation="MAGFSKVVATIFLMMLLVFATDMMAEAKICEALSGNFKGLCLSS
                     RDCGNVCRREGFTDGSCIGFRLQCFCTKPCA"
     sig_peptide     join(105..168,1017..1033)
                     /evidence=experimental
     intron          169..1016
                     /number=1
     exon            1017..>1180
                     /number=2
     mat_peptide     1034..1177
                     /evidence=experimental
BASE COUNT      475 a    187 c    259 g    469 t
ORIGIN
        1 gaaaagtcta tatatattta gtagtggcgt cttcattctt tacccacata gaagaaatag
       61 aaattattat gctgaaaaaa aaaaaaagat ccaaaaatat tattatggct ggcttttcca
      121 aagtagttgc aactattttt cttatgatgt tgctggtttt tgctactggt aattcatttt
      181 cttttctttt tttccaaagt agttcaacta atggtatgac gatatttaat acaacaacat
      241 atcaagtact aaaatttcac aagtgaggtc tgaaaaaggt aaagtgtata cagaatatat
      301 atataaaacc tatttcttca ctcgtttgag actctctata ggtaaattta aagcggattc
      361 tgcaagttca actaatggta tgacggtatt taatacaaca ataatatgtc agtagtaaat
      421 tttcacgagt gaggtctgag aaaggtaaag tgtacatagg tttaatttta ccattacttc
      481 ggagcagtgg cggaaccaga aatttgatga acatagtgta aattgcagtt gcttcaaggg
      541 agagcaaaat tacatatata ctcataatag ccaacattta acctatatac acaatacatt
      601 tttttgatga agggtatgca cttgaccacc ttttaatata ggtggctctg ctcatgcttt
      661 ggagaagtag aaaagcagtt ttcaaaagac cctcggccca agtgagattt aataggaaat
      721 gaaatttaaa tttaaaaaaa aaaatgaatg ctttttggaa tttgtaatcc aaaagtaagg
      781 aaccaaaagt cttattatcc ctaagcatat aacatatact gaacaataat aatagcaaag
      841 gtggatgtac catacgaaat aagttgatat cgaatttatc ggaattcaat atttttgatt
      901 gagctcatat atttatatgt aaataactcc atgactatca caacaaatag taatattaaa
      961 gtaaaaactt aattatattc tgaatctact tctgtatatt ttacgttgat atacagatat
     1021 gatggcggag gcaaagatct gcgaggcgtt gagcggcaac ttcaaggggt tgtgccttag
     1081 tagccgcgat tgtggtaatg tttgccgtag agagggattt accgatggct cttgcattgg
     1141 attccgtctt caatgcttct gcacgaagcc ctgtgcttaa ttaactcttg agaggtgaaa
     1201 gtctggatgg atagattgaa aaaagataaa ttactatgaa ttaatgagta tttatagttt
     1261 gttgttgtgc ttttatttgt catgaaataa agaccatttg gattaatggt tgctatggaa
     1321 aaaaagttgt tgtcaacttt tgattgtaag ttttttgttt ggaaggttgt tatctaaagt
     1381 attgtatcgt
//
LOCUS       CAGTHIOGN    5461 bp    DNA             PLN       20-MAR-1998
DEFINITION  Capsicum annuum defensin gene.
ACCESSION   X95730
VERSION     X95730.1  GI:1200227
KEYWORDS    defensin.
SOURCE      Capsicum annuum.
  ORGANISM  Capsicum annuum
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            euphyllophytes; Spermatophyta; Magnoliophyta; eudicotyledons;
            Asteridae; Solananae; Solanales; Solanaceae; Capsicum.
REFERENCE   1  (bases 1 to 5461)
  AUTHORS   Meyer,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-1996) B. Meyer, Institut de Biologie Moleculaire
            des Plantes, 12 rue du Gl Zimmer, F-67000 Strasbourg, FRANCE
REFERENCE   2  (bases 1 to 5461)
  AUTHORS   Meyer,B., Houlne,G., Pozueta-Romero,J., Schantz,M.L. and Schantz,R.
  TITLE     Fruit-specific expression of a defensin-type gene family in bell
            pepper. Upregulation during ripening and upon wounding
  JOURNAL   Plant Physiol. 112 (2), 615-622 (1996)
  MEDLINE   97037730
FEATURES             Location/Qualifiers
     source          1..5461
                     /organism="Capsicum annuum"
                     /cultivar="Yolo wonder"
                     /db_xref="taxon:4072"
                     /clone_lib="Lambda gem 11"
                     /clone="jl-2"
     exon            105..168
                     /number=1
                     /evidence=experimental
     sig_peptide     join(105..168,1294..1310)
     sig_peptide     join(105..168,5068..5084)
     CDS             join(105..168,1294..1454)
                     /note="ji"
                     /codon_start=1
                     /protein_id="CAA65046.1"
                     /db_xref="GI:2980832"
                     /db_xref="SPTREMBL:O65740"
                     /translation="MAGFSKVIATIFLMMMLVFATGMVAEARTCESQSHRFKGLCFSK
                     SNCGSVCHTEGFNGGHCRGFRRRCFCTRHC"
     CDS             join(105..168,5068..5231)
                     /note="j2"
                     /codon_start=1
                     /product="defensin"
                     /protein_id="CAA65045.1"
                     /db_xref="GI:1200228"
                     /db_xref="SPTREMBL:Q39455"
                     /translation="MAGFSKVIATIFLMMMLVFATDMMAEAKICEALSGNFKGLCLSS
                     RDCGNVCRREGFTSGVCRGFPLKCFCRKPGA"
     intron          169..5067
                     /number=1
     mat_peptide     1311..1451
                     /note="Ji protein"
                     /evidence=experimental
                     /product="defensin"
     exon            1311..1454
                     /note="exon 2ji"
                     /number=2
                     /evidence=experimental
     polyA_site      1572
                     /evidence=experimental
     polyA_site      1626
                     /evidence=experimental
     polyA_site      1707
                     /evidence=experimental
     exon            5068..5231
                     /note="exon 2j2"
                     /number=2
     mat_peptide     5085..5228
                     /note="J2 protein"
                     /product="defensin"
BASE COUNT     1740 a    832 c    998 g   1889 t      2 others
ORIGIN
        1 cgccctactc aacaattttt gctactataa attagaccta tttgctaata ttttcattca
       61 ctcttcacta cataaaataa aaataaaaga agagatatac taatatggct ggtttttcca
      121 aagttattgc aactattttc cttatgatga tgttggtttt tgctactggt aattcatttt
      181 cttttcttta atttcatgtt tggttcttga tatatatata taaaaaagat attaattctt
      241 gtataatttg aaaaaaaaaa attaacttta taagttcata gtttatagtc acacaaatat
      301 atgacagttt atcattataa gtttcaaata ttatatagtt acataaatat taagacatat
      361 ttaaaattgc aagttgtaaa ggccttttat ttttctgaat ttatcgtttt caattcaaat
      421 tatataaaag tatggaataa gaaaaaactt gtactgatat atagtaagat tatacatatt
      481 tttcaaacta tttgttagag ttttcaaact atttgttaga gttatgatta tttcatttaa
      541 aagtttaaac ggttagagcg aacacttata ttaaaataat tgtatattct tctcgtatgc
      601 gacttcacat gcggatacgt tcctttttca tgagcaagca cgtgaaattt ctctttgata
      661 atggataacg tgagattcta acctacgact atgctgtcta cgaccctatg cttggtcctt
      721 accgaccata ttgaaacgtg cagttatatc atttaaaagt ttaaattata taagaatgaa
      781 tatacactta tatttattta gttatatatt ctttcgctcg aacatacact agtgactcta
      841 gattctgaat ttgcctctaa aatttacaca cctaatagtt catgtataat tcttttttat
      901 gagccaaaca cgtgaagttt ttctttgata atagataata gtgcgattct aagcctcagg
      961 ggcggagcca cctttggaca agggggttca tccgaatctc ctttcggtgg aaaattatat
     1021 tatttatgca tgattaaaat tattttttat gtatctatag tagatgtcgt acctccttcg
     1081 gctagttcgt atgttcactt ctgaaccccc ttagtgaaaa tcctagctcc accactgttg
     1141 atcctcgact agtatgttaa aacgtgcaat catttcattt aaaagtttaa attgtaaaaa
     1201 gaaacacgct catattcact taattacata ttctttgctc gactgctact gactctagat
     1261 cctgaatttg cctgcacgtt aataattgta cagggatggt cgcggaggca aggacttgtg
     1321 agtcgcagag ccataggttc aagggtttgt gcttcagtaa gagcaactgt ggttccgttt
     1381 gccacaccga gggctttaac ggaggccact gccgtggctt tcgccgccgt tgcttctgca
     1441 ctcgacactg ttaaattaat atcattaatt aactttctgg atcgatgttg atgtttttta
     1501 tatgagggtt tttggtgtgc aatatgaatt aatgtcatta ataaactttc tggatcgagg
     1561 ttgatgtttt ttatatgagg gtttttggtg tgtaatgtga attaatatca ttaattaact
     1621 ttctagatcg acgttggtgt tgactgtgaa tgttttttat gtgtaatatg aattataatg
     1681 tcataaaata agagttggtt aatgactatt ttaacctcaa gaaatagaat tgtttggttc
     1741 tattttcttg ggagttggtg tgttgttatg gttaatattg ttggctattt ttttattgca
     1801 aatttcttta atattgtgag aatttaaaat ttatgagttt ttaataaatt tatcgacctt
     1861 tttagttatt gatttgtaat caaatattta tgcgtattta gtttattacc tattacaaat
     1921 acaagagcta agcaaaaatt actgaatttg tagctctccc caatggcggc gccagaaatt
     1981 ttattaagaa catcgaaata caaaaaagaa aatgcgcgaa aaaacctcta aaataaattt
     2041 tactaatgaa agtagaaata aatattgtat ctgctttaaa aacacataca tacacacaca
     2101 catatatata tatatatacc cgtagtggat cgcacataca tacatataca tttataatta
     2161 gatacatgca atttcagtta gaatgagata tcgtatacac acaaatacaa tatattcctg
     2221 aaattaaaaa gttactactt ataaaatatt aagttgtatc tattaacaag gggttaagtt
     2281 cagaaatgac cctaaaatgg gttgattttg atattttggc gcccttagaa aagtcttaca
     2341 aaaatagcct ccacgagggg tgggtcttaa gtttttagcc cccctaatat ttatgaacat
     2401 ttcaaggaga tatggaacta gcataaattg atagaccaaa agttttgtct tataggaaaa
     2461 cattagaact tagatatgtt gtccataagg caggcgttct aactcttaca cataaggcac
     2521 acaagttttt aggggcggac ctatgtagga ttttggggtg ctccggcccc actaaactcg
     2581 atacagaata ggtataatta catagaaaat atagggaaat aggtataaag tatataaaag
     2641 cacccactat aataaaaagt tgggtgggtg cattgacatt taggttgatc ttaaggtgct
     2701 tcattggaag gatgtctcgg gtttgaacct catttaaatg atttttttct ttttttggtt
     2761 tttaaaattt ttaagcaccc actatcctta aatcttgcgt ccgccactgt aagtttcccc
     2821 tcataaccaa acataagaac ttatttagag tggcctaaaa tccaagtcgg catcacactt
     2881 tggagnatat cgattcaaga tcagatattt gaggaacaaa aattcaacat cgaacacttt
     2941 gagggacact ggatgtaatt tcatagtaag ttagtccaaa ggcccaattc aaatagtcca
     3001 ttattgatta ctcctaagcc catttggaag aagtgaagta gaatacaaat gagttccttt
     3061 ttatcttgtt gggactttta actatgtggc atgtcctttt tggctgattg ggaaaaaaat
     3121 gccctattgg atcaaaaatt tggggggttt aggggcctga taggtacaag ccttagttga
     3181 gaggtctaag tgaatttacg aaacaagttt aagggcttgt cgatgggttt aatttattac
     3241 atgagttttc aacttgttat gtttgataag aaccaaatta tatataatac tatgttcaat
     3301 taaaataagt tagtttttct tcagaatgac ttagttggtt taaaggatgg ttatccatac
     3361 gtatgacctg ggttcgattc cccctaaatg ccttttgagt cgagcctgtc gcacagaact
     3421 tgccttgtgc ggtttacatc ttctgtgtgg tttgccagct attacacaat tgaggattta
     3481 ttcggtgcgc acaaagtcgt atgatgagat agttgatgga ccaatttgca caacctgttc
     3541 taaaaaaata ttatgccact ctgaacggag tgacggtcca atgtgcctca ttgaagggcg
     3601 gacggtccaa tttaaatgta acatgtcact ttgaacggag taacgatgta acgtgtctca
     3661 ttaaagggca ggcgattcaa tttaaacgta acatgccact ttgaacggag tgacggtcca
     3721 acgtgtctca attaagggcg gacgatccaa tttaaatgta acatgccact ttgaatggag
     3781 tgacggtcct tgaatcgagg gtttatccgg ttgcacaaag tgctcaccac agagtgctca
     3841 ctgaaaagta aaggttgtga cagagattgt agcgcctgta gtcgtacaca cacatatata
     3901 tatgtccggt gcgcacaaag tgctcaccaa agaatagaga ttgtgacaaa gattgtagcg
     3961 accacatata tatatgtata tgtatatncg accacctttt ttttttttat tgtgcatgca
     4021 agggatagag agctctatat atagattagc tgccttctat atatggcatg catgtaccta
     4081 tcattcagtg gctgcatcac atacactaca cttcatttat acatcatatt agtaagtaag
     4141 atatgtagat aattaaggat agagatattt acataagaag acaaagataa ggaattaacc
     4201 ggccaacctt tggaaggtta aaggtttcat aggttttaat ttatttatct agttttgatt
     4261 tgttacgaag cttcagaaag caaaagaaga cttaaatttg tgtttataaa ctatgcgatt
     4321 ttttacatta atacgacagt tagaatttac gaagacaaaa ttctcccgat cgatctttct
     4381 atcttgtgag gattttcgat gatcataaca caaagtcatg tataatttct gagtctattt
     4441 tcgaaagaaa tactcatagt tcctactata tctatggtcc atctggtcaa tgctacagat
     4501 aataaatcag tgatcgtgac tgctacagac tgctcgttga tctccatgca tgtataaagt
     4561 agatctccaa gttcaactga tggtgtgaca tgagtattta atataacagc agatcaagta
     4621 gtaaaatctc gcaagtaaag tatggaaagg gtaaaatata tataggtctt actactacct
     4681 ccgggaggta gagaggcagg ctttcggaag gaccctcgac tcacttgtgg gatttaataa
     4741 ggaacaatgg aaatttaaac ttaaaaaatg gaatggtttt ttggattttg gtaatccaaa
     4801 agtaagggaa ccaagagtgt cattgtccct aaacgtatac catagactat acaataataa
     4861 taataataat ttataataat aataatagca aaggtggatg catgtaacat gctaatgtcg
     4921 atatttgatt catctaaatt caatattttg attgagctca tatatttata tgtaaattac
     4981 tccatgacta ttacaacaaa tagtaatatt aaagtaaaaa tttaattaaa ttctgaattt
     5041 actctgacat tttacgttga tatacagata tgatggcgga ggcaaagatc tgcgaggcgt
     5101 tgagcggcaa cttcaagggg ttgtgcctta gtagccgcga ttgtggtaat gtttgccgta
     5161 gagagggatt taccagtggc gtttgccgcg gatttcctct taaatgcttc tgcaggaagc
     5221 ccggtgctta attaactctc gagaggtgaa agtctggatg gatagattga aatttttttt
     5281 tactatgaat taattgtttt tttttttttt tttttttata gtttggttat tgtgctttta
     5341 tttgtcacga aataagatca tttggattaa tggttgctat ggtaaaaaag ttgttgtcaa
     5401 cttttgattg taagtttctt tttcgtttgg aaaattataa aaataatcaa ttggtcttaa
     5461 a
//
LOCUS       GMU12150      527 bp    mRNA            PLN       22-DEC-1998
DEFINITION  Glycine max Essex protease inhibitor mRNA, complete cds.
ACCESSION   U12150
VERSION     U12150.1  GI:533691
KEYWORDS    .
SOURCE      soybean.
  ORGANISM  Glycine max
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            euphyllophytes; Spermatophyta; Magnoliophyta; eudicotyledons; core
            eudicots; Rosidae; eurosids I; Fabales; Fabaceae; Papilionoideae;
            Glycine.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Maitra,N. and Cushman,J.C.
  TITLE     Characterization of a Drought-Induced Soybean cDNA Encoding a Plant
            Defensin (Accession No. U12150) (PGR98-213)
  JOURNAL   Plant Physiol. 118 (4), 1536 (1998)
REFERENCE   2  (bases 1 to 509)
  AUTHORS   Cushman,J.C. and Maitra,N.
  TITLE     Isolation and expression of a drought-induced protease inhibitor
            from soybean
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 527)
  AUTHORS   Cushman,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JUL-1994) John C. Cushman, Biochemistry and Molecular
            Biology, Oklahoma State University, 350 Noble Research Center,
            Stillwater, OK 74078, USA
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Glycine max"
                     /strain="Essex"
                     /db_xref="taxon:3847"
                     /clone="Pin1 (Dhn8)"
                     /clone_lib="Lambda Uni-Zap XR"
                     /tissue_type="leaves"
                     /dev_stage="3 weeks old"
     5'UTR           1..46
     CDS             47..286
                     /note="similar to product encoded by  potato: GenBank
                     Accession Number M22140, and Swiss-Prot Accession Number
                     P20346"
                     /codon_start=1
                     /product="protease inhibitor"
                     /protein_id="AAC97524.1"
                     /db_xref="GI:533692"
                     /translation="MSRSVPLVSTICVLLLLLVATEMMGPTMVAEARTCESQSHRFKG
                     PCLSDTNCGSVCRTERFTGGHCRGFRRRCFCTKHC"
     3'UTR           287..509
     polyA_signal    500..503
BASE COUNT      126 a    105 c    110 g    186 t
ORIGIN
        1 ctattaccca ctacgttgct ttcttggaac tttgttactc tcacacatgt ctcgctccgt
       61 gcctttggtt tcaaccattt gtgtcttgct tctgcttctg gtggccactg agatgatggg
      121 gccaacaatg gtggcagaag caagaacttg tgagtctcag agccaccgtt tcaaggggcc
      181 atgtttgagt gacaccaact gtggctctgt ttgccgaacc gaacgtttca ctggaggaca
      241 ctgccgtggc ttccgtcgca gatgcttctg caccaaacat tgttaatttt aaaaaattaa
      301 ttaagagtgc tttgatagaa gatgatagat ctatcatctt tctactcgtg ttgttaatta
      361 accactttgt gatatgagaa taagggttac ccattctact atgggtttct ttgtttgcct
      421 cttattttat ctagttagag atccttttat gaaacgagtg atcagtgtga aggaattatg
      481 ttatctgtgt tgttaagtta atattattct agtaaatttt actccac
//
LOCUS       NTDEFENSI     486 bp    mRNA            PLN       30-JUL-1999
DEFINITION  N.tabacum mRNA for defensin.
ACCESSION   X99403
VERSION     X99403.1  GI:4582687
KEYWORDS    defensin; NTS13 gene.
SOURCE      common tobacco.
  ORGANISM  Nicotiana tabacum
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            euphyllophytes; Spermatophyta; Magnoliophyta; eudicotyledons; core
            eudicots; Asteridae; euasterids I; Solanales; Solanaceae;
            Nicotiana.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Li,H.Y. and Gray,J.E.
  TITLE     Molecular Characterization of a cDNA, NTS13, Encoding a
            Defensin-like Protein in Tobacco Styles (Accession No. X99403).
            (PGR99-071)
  JOURNAL   Plant Physiol. 120 (2), 633-633 (1999)
REFERENCE   2  (bases 1 to 486)
  AUTHORS   Li,H.Y.
  JOURNAL   Thesis (1996) University of Sheffield
REFERENCE   3  (bases 1 to 486)
  AUTHORS   Li,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUL-1996) H. Li, University Of Sheffield, Molecular
            Biology And Biotechnology, P.O.Box 594, Firth Court, Western Bank,
            Sheffield, S10 2UH, UK
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Nicotiana tabacum"
                     /cultivar="W38"
                     /sub_species="Winsconsin 38"
                     /db_xref="taxon:4097"
                     /dev_stage="style"
                     /lab_host="E.coli"
                     /clone="NTS13"
     mRNA            <1..>486
                     /gene="NTS13"
                     /function="expressed in mature flower tissue-stigma,
                     style, and petal"
                     /product="defensin"
     gene            1..486
                     /gene="NTS13"
BASE COUNT      119 a     96 c    112 g    159 t
ORIGIN
        1 ctcgagttct tctctctttc aaagatctgt ctggggaaat ggcaaactcc atgcgcttct
       61 ttgcaactgt gttacttata gcattgcttg tcacggctac cgagatggga ccaatgacaa
      121 ttgcagaggc aagaacttgt gagtcccaga gccaccgttt caagggacca tgctcaagag
      181 atagcaactg tgccaccgtt tgtctgaccg aaggattttc cggtgggcga tgcccgtgga
      241 ttcccccccg ttgtttctgt accagccctt gctaattgag aatattatcc cttaattatg
      301 tatgtaaaag tcacttaagt tggaataagc actatgattg ttcaaagatg gacgtgctag
      361 ctagtttaat ttcttaaact agttgtcgtg atctttgatt gtatgcaatt atggtggtag
      421 aaaggtatcc aatttcggtg ttgtactttt ggttttaagt aacgttatga ataatggttt
      481 cggctt
//
LOCUS       ZMA133530     406 bp    mRNA            PLN       02-DEC-1999
DEFINITION  Zea mays mRNA for BETL3 protein.
ACCESSION   AJ133530
VERSION     AJ133530.1  GI:5042330
KEYWORDS    betl3 gene; BETL3 protein; defensin protein.
SOURCE      Zea mays.
  ORGANISM  Zea mays
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            euphyllophytes; Spermatophyta; Magnoliophyta; Liliopsida; Poales;
            Poaceae; Zea.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Hueros,G., Royo,J., Maitz,M., Salamini,F. and Thompson,R.D.
  TITLE     Evidence for factors regulating transfer cell-specific expression
            in maize endosperm
  JOURNAL   Plant Mol. Biol. 41, 403-414 (1999)
REFERENCE   2  (bases 1 to 406)
  AUTHORS   Thompson,R.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAR-1999) Thompson R.D., Plant Breeding, MPI For
            Plant Breeding Research, Carl-von-Linne-weg 10, D-50829 Koeln,
            GERMANY
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Zea mays"
                     /variety="A69Y"
                     /db_xref="taxon:4577"
                     /country="Argentina"
     sig_peptide     50..97
                     /gene="betl3"
     CDS             50..295
                     /gene="betl3"
                     /note="defensin-like protein"
                     /codon_start=1
                     /product="BETL3 protein"
                     /protein_id="CAB44663.1"
                     /db_xref="GI:5042331"
                     /db_xref="SPTREMBL:Q9XGD9"
                     /translation="MRIVYMAAVMCLVLATMSSTSPSFCQAGGCIGCPRAPPPPSDET
                     CYEDLKCSASRCHLGCIHRGYKGTGSYCRGRDCCCKH"
     gene            50..295
                     /gene="betl3"
     mat_peptide     95..292
                     /gene="betl3"
                     /product="BETL3 protein"
BASE COUNT      104 a    102 c    102 g     98 t
ORIGIN
        1 cctgcatata ctaacactgc aaaactgaaa agggtgatag caaacgacaa tgaggattgt
       61 gtacatggca gctgttatgt gtttggttct tgcaacaatg tcttctacct ccccatcatt
      121 ctgccaagct gggggctgca tcggctgccc acgggcccca ccaccgccgt ccgatgagac
      181 atgctacgag gacctgaagt gttcggcttc gaggtgccac ctgggttgca tacacagggg
      241 ctacaagggt acgggttcgt actgccgtgg gagggactgc tgctgcaaac attgatgcac
      301 cgtagtgcat ctatgcattt catggcctgg ccatacaata aacctagatg tgtatccttt
      361 gcctccgacg actataaatg aaatgaaata ctactaaggt tcaaaa
//
