LOCUS       ST322R                   470 bp    mRNA    linear   PLN 18-APR-2005
DEFINITION  Potato mRNA for tuber protein (p322), put. proteinase inhibitor.
ACCESSION   X13180 M22140
VERSION     X13180.1  GI:21393
KEYWORDS    proteinase inhibitor; tuber protein.
SOURCE      Solanum tuberosum (potato)
  ORGANISM  Solanum tuberosum
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliophyta; eudicotyledons; core eudicots;
            asterids; lamiids; Solanales; Solanaceae; Solanum.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Stiekema,W.J., Heidekamp,F., Dirkse,W.G., van Beckum,J., de
            Haan,P., ten Bosch,C. and Louwerse,J.D.
  TITLE     Molecular cloning and analysis of four potato tuber mRNAs
  JOURNAL   Plant Mol. Biol. 11, 255-269 (1988)
COMMENT     On Feb 24, 2005 this sequence version replaced gi:169579.
            p322 mature product AA seq shows 30% homologyto that of soybean
            Bowman-Birk proteinase inhibitor
            Data kindly reviewed (21-Jul-1989) by Stiekema W.J.
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Solanum tuberosum"
                     /mol_type="mRNA"
                     /cultivar="Bintje"
                     /db_xref="taxon:4113"
                     /clone="p322."
                     /clone_lib="pBR322"
     CDS             7..231
                     /note="unnamed protein product; put. preprotein (AA -27 to
                     47)"
                     /codon_start=1
                     /protein_id="CAA31577.1"
                     /db_xref="GI:21394"
                     /db_xref="GOA:P20346"
                     /db_xref="InterPro:IPR003614"
                     /db_xref="InterPro:IPR008176"
                     /db_xref="InterPro:IPR008177"
                     /translation="MRFFATFFLLAMLVVATKMGPMRIAEARHCESLSHRFKGPCTRD
                     SNCASVCETERFSGGNCHGFRRRCFCTKPC"
     sig_peptide     7..87
                     /note="putative"
     mat_peptide     88..228
                     /product="putative mature protease inhibitor"
     repeat_unit     231..242
                     /note="inverted repeat A"
     repeat_unit     244..255
                     /note="inverted repeat A"
     polyA_site      470
                     /note="polyA site"
ORIGIN      
        1 ctatccatgc gtttctttgc tactttcttt cttctagcta tgcttgtcgt ggctactaag
       61 atgggaccaa tgagaattgc agaggcaaga cattgcgagt cgttgagcca tcgtttcaag
      121 ggaccatgta cgagagatag caattgtgct tcggtctgtg agaccgaaag attttccggt
      181 ggcaattgcc atggattccg tcgccgttgc ttttgcacta agccatgcta aatgagtatt
      241 aaaaattatg tgtaatagaa gaagtttgag aaaaaaatta tgtactcttg aataaagtac
      301 actatgattg ttcaaagata tatgtggtgc tagttttgtt tgtaaaacta gtcgtgatct
      361 ttgaatttat atgcaattat ggtgcactag acttgttaat ttcttcatgt gatgtatttt
      421 ttgctctttt gttatgaaat attatggata aaatttgtct tttagtcttt
//
LOCUS       PETGTHIONI              1561 bp    DNA     linear   PLN 04-APR-1995
DEFINITION  Petunia inflata gamma-thionin homolog gene, complete cds.
ACCESSION   L27173
VERSION     L27173.1  GI:499654
KEYWORDS    gamma-thionin.
SOURCE      Petunia integrifolia subsp. inflata
  ORGANISM  Petunia integrifolia subsp. inflata
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliophyta; eudicotyledons; core eudicots;
            asterids; lamiids; Solanales; Solanaceae; Petunia.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Karunanandaa,B., Singh,A. and Kao,T.H.
  TITLE     Characterization of a predominantly pistil-expressed gene encoding
            a gamma-thionin-like protein of Petunia inflata
  JOURNAL   Plant Mol. Biol. 26 (1), 459-464 (1994)
  MEDLINE   95036017
   PUBMED   7948892
COMMENT     Original source text: Petunia inflata pistil DNA.
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Petunia integrifolia subsp. inflata"
                     /mol_type="genomic DNA"
                     /sub_species="inflata"
                     /db_xref="taxon:212142"
                     /tissue_type="pistil"
     TATA_signal     542..548
     exon            <693..794
                     /number=1
     5'UTR           <693..730
     CDS             join(731..794,1127..1299)
                     /note="gamma-thionin homolog"
                     /codon_start=1
                     /protein_id="AAA64740.1"
                     /db_xref="GI:499655"
                     /translation="MGRSIRLFATFFLIAMLFLSTEMGPMTSAEARTCESQSHRFHGT
                     CVRESNCASVCQTEGFIGGNCRAFRRRCFCTRNC"
     mat_peptide     1156..1296
                     /product="gamma-thionin homolog"
     intron          795..1126
                     /number=1
     exon            1127..1410
                     /number=2
     3'UTR           1296..1410
     polyA_signal    1376..1381
     polyA_site      1411
ORIGIN      
        1 aagcttaact actaatgtat acacacactc aaagaatttt acttctaaaa gatgtggtag
       61 aatattaagc tagcgtttgg acctagatct tgttgaaact tgagaatttg agtttttgca
      121 atttagttga aaaataattt ttgaaacttc aaattgtgtt tggacataca ttttacttga
      181 aaaaaaattg aaagtactta ttatccttaa ctaaaattat tgagtacgag tcttgaaaat
      241 aaagtcgttt tttataacga atgcacgagt cctttaaagt gggactttca atcacgaatc
      301 ctcaataatg gaaccaaaaa taattgctag acaccaggtt agaaaccaaa gcaaaacaat
      361 attttaacac tctcggcgac gaaataatta caggcaattt ttccaacaga atgagtaatt
      421 acttaaggaa tagagcagat tacatgtgac acctaattaa aatacagtaa taggagcaag
      481 tataaaaaca cttacatggg catgataaag aaattaagta gaactgtgct tggcatagct
      541 agctagtata tcctttcttt aagctgctaa cactggactg acgtattatt acaagtaacc
      601 atgcgcagaa ttctttaacc acatactcgt aaataagcgc ctataaaaca ccccatatgc
      661 tagttccacc ttgctagtac taaacttctt tccacattaa tctcccttct ctctctttct
      721 ctgtgtgaag atgggccgct ccattcgttt gttcgcaact ttcttcctta ttgcaatgct
      781 atttttgtcc actggttcgt ctcagatctt ccttgttaac gttcttttct tatactactt
      841 ctatcacatg tagcacatag ttcttcaatt aaagacgcac cctttaacca tgttgagttt
      901 ctttctttat tgcaacaaca ggcggtcaat ctattgaata tcaatgcata tttatttgaa
      961 atttaagtta ttacttatat acactatagt agtcgtattt atttttatag gttaaaagat
     1021 agaacgattg ttattattat actgtaacaa taacaccata tggtcttatt atgtattatt
     1081 cctgtaatat ctgattcttg attttaattt tgcttactta ttccagagat gggaccaatg
     1141 acaagtgcag aggcaaggac ctgtgagtca cagagccacc gtttccatgg aacatgtgtt
     1201 agggagagca actgtgcttc cgtttgccag acagagggct tcatcggcgg caactgccgt
     1261 gcgttccgcc gtcgttgctt ctgcaccaga aactgttaga aaatactaag aaagttgtta
     1321 gtgaaatgac gctgttcctg taatactgta gtagtttctt ttctaaattc cgatgaataa
     1381 agactatctc acacatggtg gtgtttagtt atggttgtat gtattctatt tctatgtctc
     1441 tctttttttc ggtgctggtt atctattgaa cgataattaa tcatatttat tagttattac
     1501 tatctccgct ccaatttaaa tattttattt ttattttagt atgtcttata aaagactagt
     1561 t
//
