GTestContradiction in primer_task definitionContradiction in primer_task definitionUnrecognized PRIMER_TASKUnrecognized PRIMER_TASKIllegal TARGET value: %1Illegal TARGET value: %1Illegal PRIMER_DEFAULT_PRODUCT value: %1Illegal PRIMER_DEFAULT_PRODUCT value: %1Illegal PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION value: %1Illegal PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION value: %1Illegal INCLUDED_REGION value: %1Illegal INCLUDED_REGION value: %1Illegal EXCLUDED_REGION value: %1Illegal EXCLUDED_REGION value: %1Missing SEQUENCE tagMissing SEQUENCE tagError in sequence quality dataError in sequence quality dataSequence quality data missingSequence quality data missingPRIMER_PAIRS_NUMBER is incorrect. Expected:%2, but Actual:%3PRIMER_PAIRS_NUMBER is incorrect. Expected:%2, but Actual:%3%1 is incorrect. Expected:%2,%3, but Actual:NULL%1 is incorrect. Expected:%2,%3, but Actual:NULL%1 is incorrect. Expected:NULL, but Actual:%2,%3%1 is incorrect. Expected:NULL, but Actual:%2,%3%1 is incorrect. Expected:%2,%3, but Actual:%4,%5%1 is incorrect. Expected:%2,%3, but Actual:%4,%5%1 is incorrect. Expected:%2, but Actual:%3%1 is incorrect. Expected:%2, but Actual:%3Primer3DialogMainОсновные параметрыTargetsЦелевые регионы<html><head/><body><p>Primer oligos may not overlap any region specified in this tag. The associated value must be a space-separated list of</p><p><span style=" font-weight:600;">start,length</span></p><p>pairs where start is the index of the first base of the excluded region, and length is its length. This tag is useful for tasks such as excluding regions of low sequence quality or for excluding regions containing repetitive elements such as ALUs or LINEs.</p></body></html><html><head/><body><p>Олиго праймера не могут перекрывать регионы, указанные в этом теге.</p></body></html><html><head/><body><p>If one or more Targets is specified then a legal primer pair must flank at least one of them. A Target might be a simple sequence repeat site (for example a CA repeat) or a single-base-pair polymorphism. The value should be a space-separated list of </p><p><span style=" font-weight:600;">start,length</span></p><p>pairs where start is the index of the first base of a Target, and length is its length.</p></body></html><html><head/><body><p>Если указаны один или больше целевых регионов то пара праймеров должна обрамлять как минимум один из них.</p></body></html><html><head/><body><p>A list of product size ranges, for example:</p><p><span style="font-weight:600;"> 150-250 100-300 301-400 </span></p><p>Primer3 first tries to pick primers in the first range. If that is not possible, it goes to the next range and tries again. It continues in this way until it has either picked all necessary primers or until there are no more ranges. For technical reasons this option makes much lighter computational demands than the Product Size option.</p></body></html><html><head/><body><p>Список диапазонов размера продукта, например:</p><p><span style="font-weight:600;"> 150-250 100-300 301-400 </span></p></body></html><html><head/><body><p>This selection indicates what mispriming library (if any) Primer3 should use to screen for interspersed repeats or for other sequence to avoid as a location for primers.</p></body></html><html><head/><body><p>Этот параметр указывает какую библиотеку будет использовать Primer3.</p></body></html><html><head/><body><p>The maximum number of primer pairs to return.</p><p>Primer pairs returned are sorted by their "quality", in other words by the value of the objective function (where a lower number indicates a better primer pair).</p><p>Caution: setting this parameter to a large value will increase running time.</p></body></html><html><head/><body><p>Максимальное число пар праймеров.</p></body></html><html><head/><body><p>The maximum stability for the last five 3' bases of a left or right primer.</p><p>Bigger numbers mean more stable 3' ends. The value is the maximum delta G (kcal/mol) for duplex disruption for the five 3' bases as calculated using the Nearest-Neighbor parameter values specified by the option of 'Table of thermodynamic parameters'.</p></body></html><html><head/><body><p>Максимум стабильности для последних пяти 3' оснований левого или правого праймеров. </p></body></html><html><head/><body><p>The maximum allowed weighted similarity with any sequence in Mispriming Library.</p></body></html><html><head/><body><p>Максимально допустимое взвешенное сходство с любой последовательностью из библиотеки ошибочного праймирования.</p></body></html><html><head/><body><p>The maximum allowed sum of similarities of a primer pair (one similarity for each primer) with any single sequence in Mispriming Library. </p><p>Library sequence weights are not used in computing the sum of similarities.</p></body></html><html><head/><body><p>Максимально допустимая сумма сходств пар праймеров с любой последовательностью из библиотеки ошибочного праймирования.</p></body></html><html><head/><body><p>The maximum allowed similarity to ectopic sites in the sequence from which you are designing the primers.</p><p>The scoring system is the same as used for Max Mispriming, except that an ambiguity code is never treated as a consensus.</p></body></html><html><head/><body><p>Максимально допустимое сходство со смещенными сайтами в последовательности из которой конструируются праймеры.</p></body></html><html><head/><body><p>The maximum allowed summed similarity of both primers to ectopic sites in the sequence from which you are designing the primers.</p><p>The scoring system is the same as used for Max Mispriming, except that an ambiguity code is never treated as a consensus.</p></body></html><html><head/><body><p>Максимально разрешенное суммарное сходство обоих праймеров со смещенными сайтами в последовательности из которой конструируются праймеры. </span></p></body></html></p></body></html><html><head/><body><p>Index of the first base of a start codon.</p><p>This parameter allows Primer3 to select primer pairs to create in-frame amplicons e.g. to create a template for a fusion protein. Primer3 will attempt to select an in-frame left primer, ideally starting at or to the left of the start codon, or to the right if necessary. Negative values of this parameter are legal if the actual start codon is to the left of available sequence. If this parameter is non-negative Primer3 signals an error if the codon at the position specified by this parameter is not an ATG. A value less than or equal to -10^6 indicates that Primer3 should ignore this parameter. Primer3 selects the position of the right primer by scanning right from the left primer for a stop codon. Ideally the right primer will end at or after the stop codon.</p><p>This parameter should be considered EXPERIMENTAL. Please check the output carefully; some erroneous inputs might cause an error in Primer3.</p></body></html><html><head/><body><p>Индекс первого основания стартового кодона.</p></body></html>Pick left primerВыбрать левый праймерor use left primer belowили использовать этот левый праймерGeneral SettingsОбщие настройки<html><head/><body><p>This section covers such settings as primer size, temperature, GC and other general settings.</p></body></html><html><head/><body><p>>В этом разделе рассматриваются такие параметры, как размер праймера, температура, GC и другие общие настройки.</p></body></html>MinМинимумOptОптимумMaxМаксимумPrimer TmТ. плав. праймера<html><head/><body><p>Minimum, Optimum, and Maximum lengths (in bases) of a primer oligo.</p><p>Primer3 will not pick primers shorter than Min or longer than Max, and with default arguments will attempt to pick primers close with size close to Opt.</p><p>Min cannot be smaller than 1. Max cannot be larger than 36. (This limit is governed by maximum oligo size for which melting-temperature calculations are valid.)</p><p>Min cannot be greater than Max.</p></body></html><html><head/><body><p>Минимальная, оптимальная и максимальная длины олиго праймера.</p></body></html><html><head/><body><p>Minimum, Optimum, and Maximum melting temperatures (Celsius) for a primer oligo.</p><p>Primer3 will not pick oligos with temperatures smaller than Min or larger than Max, and with default conditions will try to pick primers with melting temperatures close to Opt.</p></body></html><html><head/><body><p>Минимальная, оптимальная и максимальная температуры плавления олиго праймера.</p></body></html><html><head/><body><p>Minimum, Optimum, and Maximum percentage of Gs and Cs in any primer or oligo.</p></body></html>
<html><head/><body><p>Минимальное, оптимальное и максимальное процентное содержание Gs и Cs в праймере или олиго.</p></body></html><html><head/><body><p>Minimum, Optimum, and Maximum percentage of Gs and Cs in any primer or oligo.</p></body></html><html><head/><body><p>Минимальное, оптимальное и максимальное процентное содержание Gs и Cs в праймере или олиго.</p></body></html><html><head/><body><p>The minimum, optimum, and maximum melting temperature of the amplicon.</p><p>Primer3 will not pick a product with melting temperature less than min or greater than max. If Opt is supplied and the Penalty Weights for Product Size are non-0 Primer3 will attempt to pick an amplicon with melting temperature close to Opt.</p></body></html><html><head/><body><p>Минимальная, оптимальная и максимальная температуры плавления ампликона.</p></body></html><html><head/><body><p>Maximum acceptable (unsigned) difference between the melting temperatures of the left and right primers.</p></body></html><html><head/><body><p>Максимально допустимое различие между температурами плавления левого и правого праймеров.</p></body></html>Table of thermodynamic parametersТаблица термодинамических параметровPrimersPrimer PairsHyb Oligos<html><head/><body><p>This section allows to set output annotations' settings.</p></body></html><html><head/><body><p>В этой секции можно задать настройки для аннотаций.</p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Specifies details of melting temperature calculation. </span></p>
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">First method uses the table of thermodynamic parameters from the paper </span></p>
<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">[Breslauer KJ, et. al (1986), Proc Natl Acad Sci 83:4746-50 http://dx.doi.org/10.1073/pnas.83.11.3746]</span></p>
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Second method (recommended) was suggested in the paper [SantaLucia JR (1998)</span></p>
<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">, Proc Natl Acad Sci 95:1460-65 http://dx.doi.org/10.1073/pnas.95.4.1460].</span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Указывает детали расчета температуры плавления. </span></p></body></html>Breslauer et. al 1986Breslauer et. al 1986SantaLucia 1998SantaLucia 1998Primer DesignerДизайнер праймеровExcluded regionsИсключенные регионы<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;">Primer oligos may not overlap any region specified in this tag. The associated value must be a space-separated list of</p>
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-weight:600;">start,length </span></p>
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;">pairs where start is the index of the first base of the excluded region, and length is its length. This tag is useful for tasks such as excluding regions of low sequence quality or for excluding regions containing repetitive elements such as ALUs or LINEs.</p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;">Олиго праймера не могут перекрывать регионы, указанные в этом теге.</p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;">If one or more Targets is specified then a legal primer pair must flank at least one of them. A Target might be a simple sequence repeat site (for example a CA repeat) or a single-base-pair polymorphism. The value should be a space-separated list of </p>
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-weight:600;">start,length </span></p>
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;">pairs where start is the index of the first base of a Target, and length is its length.</p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;">Если указаны один или больше целевых регионов то пара праймеров должна обрамлять как минимум один из них.</p></body></html>Product size rangesДиапазоны размера продукта<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;">A list of product size ranges, for example:</p>
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-weight:600;"> 150-250 100-300 301-400 </span></p>
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;">Primer3 first tries to pick primers in the first range. If that is not possible, it goes to the next range and tries again. It continues in this way until it has either picked all necessary primers or until there are no more ranges. For technical reasons this option makes much lighter computational demands than the Product Size option.</p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;">Список диапазонов размера продукта, например:</p>
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-weight:600;"> 150-250 100-300 301-400 </span></p>
</p></body></html>Mispriming/Repeat libraryБиблиотека повторов/ошибочного праймирования<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">This selection indicates what mispriming library (if any) Primer3 should use to screen for</span></p>
<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">interspersed repeats or for other sequence to avoid as a location for primers.</span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Этот параметр указывает какую библиотеку будет использовать Primer3.</span></p></body></html>Number to returnРезультатов не болееMax repeat misprimingМаксимальный повтор ошибочного праймированияMax template misprimingМаксимальный шаблон ошибочного праймированияMax 3' stabilityМаксимальная 3' стабильностьPair max repeat misprimingПарный максимальный повтор ошибочного праймированияPair max template misprimingПарный максимальный шаблон ошибочного праймирования<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">The maximum number of primer pairs to return. </span></p>
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Primer pairs returned are sorted by their "quality", in other words by the value of the objective function (where a lower number indicates a better primer pair).</span></p>
<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Caution: setting this parameter to a large value will increase running time.</span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Максимальное число пар праймеров.</span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">The maximum stability for the last five 3' bases of a left or right primer. </span></p>
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Bigger numbers mean more stable 3' ends. The value is the maximum delta G (kcal/mol) for duplex disruption for the five 3' bases as calculated using the Nearest-Neighbor parameter values specified by the option of 'Table of thermodynamic parameters'.</span></p>
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Максимум стабильности для последних пяти 3' оснований левого или правого праймеров. </span></p>
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">The maximum allowed weighted similarity with any sequence in Mispriming Library.</span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Максимально допустимое взвешенное сходство с любой последовательностью из библиотеки ошибочного праймирования.</span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">The maximum allowed sum of similarities of a primer pair (one similarity for each primer) with any single sequence in Mispriming Library. </span></p>
<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Library sequence weights are not used in computing the sum of similarities.</span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Максимально допустимая сумма сходств пар праймеров с любой последовательностью из библиотеки ошибочного праймирования. </span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">The maximum allowed similarity to ectopic sites in the sequence from which you are designing the primers. </span></p>
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">The scoring system is the same as used for Max Mispriming, except that an ambiguity code is never treated as a consensus.</span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Максимально допустимое сходство со смещенными сайтами в последовательности из которой конструируются праймеры. </span></p>
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">The maximum allowed summed similarity of both primers to ectopic sites in the sequence from which you are designing the primers. </span></p>
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">The scoring system is the same as used for Max Mispriming, except that an ambiguity code is never treated as a consensus.</span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Максимально разрешенное суммарное сходство обоих праймеров со смещенными сайтами в последовательности из которой конструируются праймеры. </span></p></body></html>Start codon positionПозиция стартового кодона<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Index of the first base of a start codon. </span></p>
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">This parameter allows Primer3 to select primer pairs to create in-frame amplicons e.g. to create a template for a fusion protein. Primer3 will attempt to select an in-frame left primer, ideally starting at or to the left of the start codon, or to the right if necessary. Negative values of this parameter are legal if the actual start codon is to the left of available sequence. If this parameter is non-negative Primer3 signals an error if the codon at the position specified by this parameter is not an ATG. A value less than or equal to -10^6 indicates that Primer3 should ignore this parameter. Primer3 selects the position of the right primer by scanning right from the left primer for a stop codon. Ideally the right primer will end at or after the stop codon.</span></p>
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">This parameter should be considered EXPERIMENTAL. Please check the output carefully; some erroneous inputs might cause an error in Primer3. </span></p>
<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;"> </span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Индекс первого основания стартового кодона. </span></p></body></html>Pick hybridization probe (internal oligo)Выбрать гибридизационную пробу (internal oligo)or use oligo belowили использовать эту oligoPick right primerВыбрать правый праймерor use right primer below (5' to 3' on opposite strand)или использовать этот правый праймер (5' в 3' в противоположном направлении)<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">This section covers such settings as primer size, temperature, GC and other general settings.</span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">В этом разделе рассматриваются такие параметры, как размер праймера, температура, GC и другие общие настройки.</span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style="-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-size:8pt;"></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style="-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-size:8pt;"></p></body></html>Primer sizeРазмер праймераProduct TmТ. плав. продуктаPrimer GC%GC состав %<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Minimum, Optimum, and Maximum lengths (in bases) of a primer oligo. </span></p>
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Primer3 will not pick primers shorter than Min or longer than Max, and with default arguments will attempt to pick primers close with size close to Opt. </span></p>
<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Min cannot be smaller than 1. Max cannot be larger than 36. (This limit is governed by maximum oligo size for which melting-temperature calculations are valid.) </span></p>
<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Min cannot be greater than Max.</span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Минимальная, оптимальная и максимальная длины олиго праймера. </span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Minimum, Optimum, and Maximum melting temperatures (Celsius) for a primer oligo. </span></p>
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Primer3 will not pick oligos with temperatures smaller than Min or larger than Max, and with</span></p>
<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">default conditions will try to pick primers with melting temperatures close to Opt.</span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Минимальная, оптимальная и максимальная температуры плавления олиго праймера. </span></p></body></html>Minimum, Optimum, and Maximum percentage of Gs and Cs in any primer or oligo.Минимальное, оптимальное и максимальное процентное содержание Gs и Cs в праймере или олиго.<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">The minimum, optimum, and maximum melting temperature of the amplicon. </span></p>
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Primer3 will not pick a product with melting temperature less than min or greater than max. If Opt is supplied and the Penalty Weights for Product Size are non-0 Primer3 will attempt to pick an amplicon with melting temperature close to Opt.</span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Минимальная, оптимальная и максимальная температуры плавления ампликона. </span></p></body></html>Max Tm differenceМаксимальная разница Т. плавMaximum acceptable (unsigned) difference between the melting temperatures of the left and right primers.Максимально допустимое различие между температурами плавления левого и правого праймеров.Max self complementarityМаксимальная комплементарностьMax 3' self complementarityМаксимальная 3' комплементарностьInside target penaltyВнутренний целевой штрафOutside target penaltyВнешний целевой штрафMax poly-XМаксимум poly-XFirst base indexИндекс первого основанияCG clampЗажим CG<html><head/><body><p>Specifies the salt correction formula for the melting temperature calculation.</p></body></html><html><head/><body><p>Задает коррекционную формулу для расчета температуры плавления.</p></body></html>Annealing oligo concentrationКонцентрация отжига олиго<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">The maximum allowable 3'-anchored global alignment score when testing a single primer for</span></p>
<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">self-complementarity, and the maximum allowable 3'-anchored global alignment score when</span></p>
<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">testing for complementarity between left and right primers.</span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Максимальная 3'-комплементарность.</span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">The maximum allowable local alignment score when testing a single primer for (local) self-complementarity and the maximum allowable local alignment score when testing for complementarity between left and right primers. </span></p>
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Local self-complementarity is taken to predict the tendency of primers to anneal to each other without necessarily causing self-priming in the PCR. The scoring system gives 1.00 for complementary bases, -0.25 for a match of any base (or N) with an N, -1.00 for a mismatch, and -2.00 for a gap. Only single-base-pair gaps are allowed.</span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Максимальная комплементарность.</span></p></body></html>Maximum number of unknown bases (N) allowable in any primer.Максимальное число неизвестных оснований (N), допустимых в праймере.The maximum allowable length of a mononucleotide repeat, for example AAAAAA.Максимальная допустимая для мононуклеотидного повтора, например AAAAAA.<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p align="justify" style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Non-default values valid only for sequences with 0 or 1 target regions. </span></p>
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<p align="justify" style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">If the primer is part of a pair that spans a target and does not overlap the target, </span></p>
<p align="justify" style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">then multiply this value times the number of nucleotide positions from the 3' end </span></p>
<p align="justify" style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">to the (unique) target to get the 'position penalty'. The effect of this parameter is </span></p>
<p align="justify" style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">to allow Primer3 to include nearness to the target as a term in the objective function.</span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p align="justify" style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Внешний целевой штраф.</span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">This parameter is the index of the first base in the input sequence.</span></p>
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Индекс первого основания во входной последовательности.</span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Require the specified number of consecutive Gs and Cs at the 3' end of both the left and right primer.</span></p>
<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">This parameter has no effect on the internal oligo if one is requested.</span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Зажим CG.</span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">The millimolar (mM) concentration of monovalent salt cations (usually KCl) in the PCR. </span></p>
<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Primer3 uses this argument to calculate oligo and primer melting temperatures. </span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Концентрация одновалентных катионов. </span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;">The millimolar concentration of divalent salt cations (usually MgCl^(2+)) in the PCR. </p>
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;">Primer3 converts concentration of divalent cations to concentration of monovalent cations using following formula:</p>
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-weight:600;">[Monovalent cations] = [Monovalent cations] + 120*(([divalent cations] - [dNTP])^0.5)</span></p>
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;">In addition, if the specified concentration of dNTPs is larger than the concentration of divalent cations then </p>
<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;">the effect of the divalent cations is not considered. </p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;">Концентрация двухвалентных катионов. </p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">The millimolar concentration of the sum of all deoxyribonucleotide triphosphates. </span></p>
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Концентрация dNTP. </span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">A value to use as nanomolar (nM) concentration of each annealing oligo over the course the PCR. </span></p>
<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Primer3 uses this argument to esimate oligo melting temperatures.</span></p>
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">The default (50nM) works well with the standard protocol used at the Whitehead/MIT Center for Genome Research --0.5 microliters of 20 micromolar concentration for each primer in a 20 microliter reaction with 10 nanograms template, 0.025 units/microliter Taq polymerase in 0.1 mM each dNTP, 1.5mM MgCl2, 50mM KCl, 10mM Tris-HCL (pH 9.3) using 35 cycles with an annealing temperature of 56 degrees Celsius.</span></p>
<p style="-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-size:8pt;"></p>
<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">The value of this parameter is less than the actual concentration of oligos in the initial reaction mix because it is the concentration of annealing oligos, which in turn depends on the amount of template (including PCR product) in a given cycle. This concentration increases a great deal during a PCR; fortunately PCR seems quite robust for a variety</span></p>
<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;"> of oligo melting temperatures. </span></p>
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Концентрация отжига олиго. </span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Non-default values valid only for sequences with 0 or 1 target regions.</span></p>
<p style="-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-size:8pt;"></p>
<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">If the primer is part of a pair that spans a target and overlaps the target, then multiply this value times</span></p>
<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;"> the number of nucleotide positions by which the primer overlaps the (unique) target to get the 'position penalty'. </span></p>
<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">The effect of this parameter is to allow Primer3 to include overlap with the target as a term in the objective function.</span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Внутренний целевой штраф.</span></p></body></html> Liberal baseРазрешить неоднозначные символыShow debuging infoПоказывать отладочную информацию<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Parameters governing choice of internal oligos are analogous to the parameters governing choice of primer pairs. </span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Параметры, регулирующие выбор внутренних олиго аналогичны параметрам, регулирующих выбор пар праймеров. </span></p></body></html>Hyb oligo excluded regionИсключаемые регионы гибридизации олиго<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Middle oligos may not overlap any region specified by this tag. The associated value must be a space-separated list of</span></p>
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt; font-weight:600;"><start>,<length></span></p>
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">pairs, where <start> is the index of the first base of an excluded region, and <length> is its length. Often one would make Target regions excluded regions for internal oligos.</span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Исключаемые олиго регионы.</span></p></body></html>Hyb oligo sizeРазмер гибридизации олигоHyb oligo TmTm гибридизации олигоHyb oligo GC%GC% гибридизации олиго<html><head/><body><p>Equivalent parameter of Primer Size for the internal oligo.</p></body></html><html><head/><body><p>Эквивалентный параметр размера праймера для внутреннего олиго.</p></body></html> <html><head/><body><p>Equivalent parameter of Primer Tm for the internal oligo.</p></body></html><html><head/><body><p>Tm олиго.</p></body></html><html><head/><body><p>Equivalent parameter of primer GC% for the internal oligo.</p></body></html><html><head/><body><p>GC% олиго.</p></body></html><html><head/><body><p>Equivalent parameter of Primer GC% for the internal oligo.</p></body></html><html><head/><body><p>GC% олиго.</p></body></html>Hyb oligo self complementarityКомплементарность гибридизации олигоHyb oligo mishyb libraryБиблиотека гибридизации олигоHyb oligo min sequence qualityМинимальное качество последовательности гибридизации олигоHyb oligo conc of monovalent cationsКонцентрация одновалентных катионов гибридизации олигоHyb oligo conc of divalent cationsКонцентрация двухвалентных катионов гибридизации олиго<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Equivalent parameter of primer max self complemntarity for the internal oligo. </span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Комплементарность олиго. </span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Equivalent parameter of primer max #Ns for the internal oligo. </span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Максимум N символов. </span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Similar to primer mispriming library, except that the event we seek to avoid is hybridization of the internal oligo to sequences in this library rather than priming from them.</span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Библиотека олиго.</span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Equivalent parameter of primer minimum quality for the internal oligo. </span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Минимальное качество последовательности олиго. </span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Equivalent parameter of primer concentration of monovalent cations for the internal oligo. </span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Концентрация одновалентных катионов олиго. </span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Equivalent parameter of primer concentration of divalent cations for the internal oligo. </span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Концентрация двухвалентных катионов олиго. </span></p></body></html>Hyb oligo max 3' self complementarityМаксимальная 3' комплементарность гибридизации олигоHyb oligo max poly-XМаксимум poly-X гибридизации олигоHyb oligo max mishybМаксимум ошибки гибридизации олиго<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Equivalent parameter of primer max 3' self complementarity for the internal oligo. </span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Максимальная 3' комплементарность олиго.</span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Equivalent parameter of primer max poly-X for the internal oligo. </span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Максимум poly-X олиго. </span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Similar to primer max library mispriming. </span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Максимум ошибки олиго. </span></p></body></html>Hyb oligo [dNTP]Гибридизация олиго [dNTP]Hyb oligo DNA concentrationДНК концентрация гибридизации олиго<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Equivalent parameter of primer concentration of the dNTPs for the internal oligo. </span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Equivalent parameter of primer concentration of the dNTPs for the internal oligo. </span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Equivalent parameter of primer DNA concentration for the internal oligo. </span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Equivalent parameter of primer DNA concentration for the internal oligo. </span></p></body></html>Self complementarityКомплементарность3' self complementarity3' комплементарностьRT-PCRRT-PCR<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-family:'MS Shell Dlg 2'; font-size:8pt;">In this section user can specify if primer product must overlap exon-exon junction or span intron. This only applies when designing primers for a cDNA (mRNA) sequence with annotated exons. </span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-family:'MS Shell Dlg 2'; font-size:8pt;">In this section user can specify if primer product must overlap exon-exon junction or span intron. This only applies when designing primers for a cDNA (mRNA) sequence with annotated exons. </span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-family:'MS Shell Dlg 2'; font-size:8pt;">This feature allows to specify if primer product must span intron-exon boundaries. </span></p>
<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-family:'MS Shell Dlg 2'; font-size:8pt;">Checking this option will result in ignoring </span><span style=" font-family:'MS Shell Dlg 2'; font-size:8pt; font-style:italic;">Excluded</span><span style=" font-family:'MS Shell Dlg 2'; font-size:8pt;"> and </span><span style=" font-family:'MS Shell Dlg 2'; font-size:8pt; font-style:italic;">Target Regions</span><span style=" font-family:'MS Shell Dlg 2'; font-size:8pt;"> from Main section.</span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-family:'MS Shell Dlg 2'; font-size:8pt;">Эта опция позволяет указать если праймер должен охватывать интрон-экзон границы.</span></p></body></html>Design primers for RT-PCR analysisДизайн праймеров для анализа RT-PCRExon annotation name:Имя экзон аннотации:<html><head/><body><p><span style=" font-size:8pt;">The name of the annotation which defines the exons in the mRNA sequence.</span></p></body></html><html><head/><body><p><span style=" font-size:8pt;">Имя экзон аннотации.</span></p></body></html>exonexonMinimum exon junction overlap sizeМинимальный размер перекрытия соединения экзонаExon range:Диапазон экзона:<html><head/><body><p><span style=" font-size:8pt;">If the range is set, primer search will be restricted to selected exons. For example:</span></p><p><span style=" font-size:8pt; font-weight:600;">1-5</span></p><p><span style=" font-size:8pt;">If the range is larger than actual exon range or the starting exon number exceeds number of exons, error message is shown. </span></p><p><br/></p></body></html><html><head/><body><p><span style=" font-size:8pt;">Если диапазон указан, то поиск будет выполнен для выделенных экзонов. Например:</span></p><p><span style=" font-size:8pt; font-weight:600;">1-5</span></p></body></html>Max number of pairs to query:Максимальное число пар для запроса:<html><head/><body><p><span style=" font-size:8pt;">This number of found primer pairs wil bel queried to check if they fullfill the requirements for RTPCR</span></p></body></html><html><head/><body><p><span style=" font-size:8pt;">Максимальное число пар для запроса</span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">In this section user can specify sequence quality of target sequence and related parameters.</span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Качество последовательности.</span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p align="justify" style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;">A list of space separated integers. There must be exactly one integer for each base in the Source Sequence if this argument is non-empty. High numbers indicate high confidence in the base call at that position and low numbers indicate low confidence in the base call at that position.</p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p align="justify" style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;">Список разделенных пробелами целых чисел.</p></body></html>Min sequence quality:Минимальное качество последовательности:The minimum sequence quality allowed within a primer.Минимальное качество последовательности.Min 3' sequence quality:Минимальное 3'качество последовательности:The minimum sequence quality allowed within the 3' pentamer of a primer.Минимальное 3'качество последовательности.Sequence quality range min:Минимум интервала качества последовательности:The minimum legal sequence quality.Минимум интервала качества последовательности.Sequence quality range max:Максимум интервала качества последовательности:The maximum legal sequence quality.Максимум интервала качества последовательности.<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">This section allows to set output annotations' settings.</span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">В этой секции можно задать настройки для аннотаций.</span></p></body></html>HelpПомощьSave settingsСохранить настройкиLoad settingsЗагрузить настройкиReset formСброс параметровPick primersПодобратьConcentration of monovalent cationsКонцентрация одновалентных катионов<html><head/><body><p>Specifies details of melting temperature calculation.</p><p>First method uses the table of thermodynamic parameters from the paper [Breslauer KJ, et. al (1986), Proc Natl Acad Sci 83:4746-50 http://dx.doi.org/10.1073/pnas.83.11.3746]</p><p>Second method (recommended) was suggested in the paper [SantaLucia JR (1998), Proc Natl Acad Sci 95:1460-65 http://dx.doi.org/10.1073/pnas.95.4.1460].</p></body></html><html><head/><body><p>Указывает детали расчета температуры плавления.</p></body></html>Max #N'sМаксимум NConcentration of divalent cationsКонцентрация двухвалентных катионовSalt correction formulaКоррекционная формула<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Specifies the salt correction formula for the melting temperature calculation.</span></p>
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Задает коррекционную формулу для расчета температуры плавления.</span></p>
<p style="-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-size:8pt;"></p></body></html>Schildkraut and Lifson 1965Schildkraut and Lifson 1965Owczarzy et. 2004Owczarzy et. 2004Concentration of dNTPsКонцентрация dNTP<html><head/><body><p>The maximum allowable 3'-anchored global alignment score when testing a single primer for self-complementarity, and the maximum allowable 3'-anchored global alignment score when testing for complementarity between left and right primers.</p></body></html><html><head/><body><p>Максимальная 3'-комплементарность.</p></body></html><html><head/><body><p>The maximum allowable local alignment score when testing a single primer for (local) self-complementarity and the maximum allowable local alignment score when testing for complementarity between left and right primers.</p><p>Local self-complementarity is taken to predict the tendency of primers to anneal to each other without necessarily causing self-priming in the PCR. The scoring system gives 1.00 for complementary bases, -0.25 for a match of any base (or N) with an N, -1.00 for a mismatch, and -2.00 for a gap. Only single-base-pair gaps are allowed.</p></body></html><html><head/><body><p>Максимальная комплементарность.</p></body></html><html><head/><body><p>Maximum number of unknown bases (N) allowable in any primer.</p></body></html><html><head/><body><p>Максимальное число неизвестных оснований (N), допустимых в праймере.</p></body></html><html><head/><body><p>The maximum allowable length of a mononucleotide repeat, for example AAAAAA.</p></body></html><html><head/><body><p>Максимальная допустимая для мононуклеотидного повтора, например AAAAAA.</p></body></html><html><head/><body><p>Non-default values valid only for sequences with 0 or 1 target regions.</p><p>If the primer is part of a pair that spans a target and does not overlap the target, then multiply this value times the number of nucleotide positions from the 3' end to the (unique) target to get the 'position penalty'. The effect of this parameter is to allow Primer3 to include nearness to the target as a term in the objective function.</p></body></html><html><head/><body><p>Внешний целевой штраф.</p></body></html><html><head/><body><p>This parameter is the index of the first base in the input sequence.</p></body></html><html><head/><body><p>Индекс первого основания во входной последовательности.</p></body></html><html><head/><body><p>Require the specified number of consecutive Gs and Cs at the 3' end of both the left and right primer.</p><p>This parameter has no effect on the internal oligo if one is requested.</p></body></html><html><head/><body><p>Зажим CG.</p></body></html><html><head/><body><p>The millimolar (mM) concentration of monovalent salt cations (usually KCl) in the PCR.</p><p>Primer3 uses this argument to calculate oligo and primer melting temperatures.</p></body></html><html><head/><body><p>Концентрация одновалентных катионов.</p></body></html><html><head/><body><p>The millimolar concentration of divalent salt cations (usually MgCl^(2+)) in the PCR.</p><p>Primer3 converts concentration of divalent cations to concentration of monovalent cations using following formula:</p><p><span style=" font-weight:600;">[Monovalent cations] = [Monovalent cations] + 120*(([divalent cations] - [dNTP])^0.5)</span></p><p>In addition, if the specified concentration of dNTPs is larger than the concentration of divalent cations then the effect of the divalent cations is not considered.</p></body></html><html><head/><body><p>Концентрация двухвалентных катионов.</p></body></html><html><head/><body><p>The millimolar concentration of the sum of all deoxyribonucleotide triphosphates.</p></body></html><html><head/><body><p>Концентрация dNTP.</p></body></html><html><head/><body><p>A value to use as nanomolar (nM) concentration of each annealing oligo over the course the PCR.</p><p>Primer3 uses this argument to esimate oligo melting temperatures.</p><p>The default (50nM) works well with the standard protocol used at the Whitehead/MIT Center for Genome Research --0.5 microliters of 20 micromolar concentration for each primer in a 20 microliter reaction with 10 nanograms template, 0.025 units/microliter Taq polymerase in 0.1 mM each dNTP, 1.5mM MgCl2, 50mM KCl, 10mM Tris-HCL (pH 9.3) using 35 cycles with an annealing temperature of 56 degrees Celsius.</p><p>The value of this parameter is less than the actual concentration of oligos in the initial reaction mix because it is the concentration of annealing oligos, which in turn depends on the amount of template (including PCR product) in a given cycle. This concentration increases a great deal during a PCR; fortunately PCR seems quite robust for a variety of oligo melting temperatures.</p></body></html><html><head/><body><p>Концентрация отжига олиго.</p></body></html><html><head/><body><p>Non-default values valid only for sequences with 0 or 1 target regions.</p><p>If the primer is part of a pair that spans a target and overlaps the target, then multiply this value times the number of nucleotide positions by which the primer overlaps the (unique) target to get the 'position penalty'.</p><p>The effect of this parameter is to allow Primer3 to include overlap with the target as a term in the objective function.</p></body></html><html><head/><body><p>Внешний целевой штраф.</p></body></html>Do not treat ambiguity codes in libraries as consensusНе относить неоднозначности кода в библиотеке к консенсусуLowercase maskingСтрочная маскировкаInternal OligoВнутреннее олиго<html><head/><body><p>Parameters governing choice of internal oligos are analogous to the parameters governing choice of primer pairs.</p></body></html><html><head/><body><p>Параметры, регулирующие выбор внутренних олиго аналогичны параметрам, регулирующих выбор пар праймеров.</p></body></html><html><head/><body><p>Middle oligos may not overlap any region specified by this tag. The associated value must be a space-separated list of</p><p><span style=" font-weight:600;"><start>,<length></span></p><p>pairs, where <start> is the index of the first base of an excluded region, and <length> is its length. Often one would make Target regions excluded regions for internal oligos.</p></body></html><html><head/><body><p>Исключаемые олиго регионы.</p></body></html>Min:Мин:<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p align="justify" style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Equivalent parameter of Primer Size for the internal oligo. </span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p align="justify" style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Эквивалентный параметр размера праймера для внутреннего олиго. </span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Equivalent parameter of Primer Tm for the internal oligo. </span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Tm олиго. </span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Equivalent parameter of primer GC% for the internal oligo. </span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">GC% олиго. </span></p></body></html>Opt:Опт:<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Equivalent parameter of Primer GC% for the internal oligo. </span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">GC% олиго. </span></p></body></html>Max:Макс:Max #NsМакс N<html><head/><body><p>Equivalent parameter of primer max self complemntarity for the internal oligo.</p></body></html><html><head/><body><p>Комплементарность олиго.</p></body></html><html><head/><body><p>Equivalent parameter of primer max #Ns for the internal oligo.</p></body></html><html><head/><body><p>Максимум N символов.</p></body></html><html><head/><body><p>Similar to primer mispriming library, except that the event we seek to avoid is hybridization of the internal oligo to sequences in this library rather than priming from them.</p></body></html><html><head/><body><p>Библиотека олиго.</p></body></html><html><head/><body><p>Equivalent parameter of primer minimum quality for the internal oligo.</p></body></html><html><head/><body><p>Минимальное качество последовательности олиго.</p></body></html><html><head/><body><p>Equivalent parameter of primer concentration of monovalent cations for the internal oligo.</p></body></html><html><head/><body><p>Концентрация одновалентных катионов олиго.</p></body></html><html><head/><body><p>Equivalent parameter of primer concentration of divalent cations for the internal oligo.</p></body></html><html><head/><body><p>Концентрация двухвалентных катионов олиго.</p></body></html><html><head/><body><p>Equivalent parameter of primer max 3' self complementarity for the internal oligo.</p></body></html><html><head/><body><p>Максимальная 3' комплементарность олиго.</p></body></html><html><head/><body><p>Equivalent parameter of primer max poly-X for the internal oligo.</p></body></html><html><head/><body><p>Максимум poly-X олиго.</p></body></html><html><head/><body><p>Similar to primer max library mispriming.</p></body></html><html><head/><body><p>Максимум ошибки олиго.</p></body></html><html><head/><body><p>Equivalent parameter of primer concentration of the dNTPs for the internal oligo.</p></body></html><html><head/><body><p>Equivalent parameter of primer concentration of the dNTPs for the internal oligo.</p></body></html><html><head/><body><p>Equivalent parameter of primer DNA concentration for the internal oligo.</p></body></html><html><head/><body><p>Equivalent parameter of primer DNA concentration for the internal oligo.</p></body></html>Penalty WeightsВеса штрафов<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">This section describes "penalty weights", which allow the user to modify the criteria</span></p>
<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">that Primer3 uses to select the "best" primers. </span></p>
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">There are two classes of weights: for some parameters there is a 'Lt' (less than) and a 'Gt' (greater than) weight. These are the weights that Primer3 uses when the value is less or greater than (respectively) the specified optimum.</span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Веса штрафов.</span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:14pt; font-weight:600;">For Primers</span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:14pt; font-weight:600;">Для праймеров</span></p></body></html>TmTmSize Размер GC%GC%Lt:Lt:<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Penalty weight for corressponding parameter of a primer less than (Lt) or greater than (Gt) the optimal value. </span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Вес штрафа для соответствующего параметра праймера меньше чем (Lt) или больше чем (Gt), что является оптимальным значением. </span></p></body></html>Gt:Gt:#N'sNMisprimingОшибочное праймированиеSequence qualityКачество последовательностиEnd sequence qualityКачество конца последовательностиPosition penaltyПозиция штрафаEnd stabilityСтабильность концаTemplate misprimingШаблон ошибочного праймирования<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Penalty weight for a primer parameter different from predefined optimum value. </span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Вес штрафа для параметра праймера отличается от оптимального предопределенного значения. </span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Determines the overall weight of the position penalty in calculating the penalty for a primer.</span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Позиция штрафа.</span></p></body></html>Product sizeРазмер продукта<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Penalty weight for corressponding parameter of a primer pair less than (Lt) or greater than (Gt) the optimal value. </span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Вес штрафа для соответствующего параметра пары праймеров меньше чем (Lt) или больше чем (Gt), что является оптимальным значением. </span></p></body></html>Tm differenceМаксимальная разница TmAny complementarityЛюбая комплементарность3' complementarity3' комплементарностьPair misprimingОшибочное парное праймированиеPrimer penalty weightВес штрафа праймераHyb oligo penalty weightВес штрафа гибридизации олигоPrimer pair template mispriming weightВес шаблона ошибочного праймирования<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Penalty weight for a primer pair parameter different from predefined optimum value.</span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Вес штрафа для параметра пары праймеров отличается от оптимального предопределенного значения.</span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Penalty weight for corressponding parameter of a internal oligo less than (Lt) or greater than (Gt) the optimal value. </span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Вес штрафа для соответствующего параметра внутреннего олиго меньше чем (Lt) или больше чем (Gt), что является оптимальным значением.</span></p></body></html>Hyb oligo #N'sN гибридизации олигоHyb oligo mishybingОшибочная гибридизация олигоHyb oligo sequence qualityКачество последовательности гибридизации олиго<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Penalty weight for an internal oligo parameter different from predefined optimum value.</span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:8pt;">Вес штрафа для параметра внутреннего олиго отличается от оптимального предопределенного значения.</span></p></body></html>mRNA sequenceПоследовательность mRNAAt 5' side (bp):Со стороны 5' (нк):At 3'side (bp)Со стороны 3' (нк)Primer product must span at least one intron on the corresponding genomic DNAПродук праймера должен охватывать по крайней мере один интрон на соответствующей геномной ДНКSequence QualityКачество последовательности<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:14pt; font-weight:600;">For Primer Pairs</span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:14pt; font-weight:600;">Для пар праймеров</span></p></body></html><html><head/><body><p>This section describes "penalty weights", which allow the user to modify the criteria that Primer3 uses to select the "best" primers.</p><p>There are two classes of weights: for some parameters there is a 'Lt' (less than) and a 'Gt' (greater than) weight. These are the weights that Primer3 uses when the value is less or greater than (respectively) the specified optimum.</p></body></html><html><head/><body><p>This section describes "penalty weights", which allow the user to modify the criteria that Primer3 uses to select the "best" primers.</p><p>There are two classes of weights: for some parameters there is a 'Lt' (less than) and a 'Gt' (greater than) weight. These are the weights that Primer3 uses when the value is less or greater than (respectively) the specified optimum.</p></body></html><html><head/><body><p>Penalty weight for corressponding parameter of a primer less than (Lt) or greater than (Gt) the optimal value.</p></body></html><html><head/><body><p>Вес штрафа для соответствующего параметра праймера меньше чем (Lt) или больше чем (Gt), что является оптимальным значением.</p></body></html><html><head/><body><p>Penalty weight for a primer parameter different from predefined optimum value.</p></body></html><html><head/><body><p>Вес штрафа для параметра праймера отличается от оптимального предопределенного значения.</p></body></html><html><head/><body><p>Determines the overall weight of the position penalty in calculating the penalty for a primer.</p></body></html><html><head/><body><p>Позиция штрафа.</p></body></html><html><head/><body><p>Penalty weight for corressponding parameter of a primer pair less than (Lt) or greater than (Gt) the optimal value.</p></body></html><html><head/><body><p>Вес штрафа для соответствующего параметра пары праймеров меньше чем (Lt) или больше чем (Gt), что является оптимальным значением.</p></body></html><html><head/><body><p>Penalty weight for a primer pair parameter different from predefined optimum value.</p></body></html><html><head/><body><p>Вес штрафа для параметра пары праймеров отличается от оптимального предопределенного значения.</p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:14pt; font-weight:600;">For Hyb Oligos</span></p></body></html><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd">
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<p style=" margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px;"><span style=" font-size:14pt; font-weight:600;">Для гибридизации олиго</span></p></body></html><html><head/><body><p>Penalty weight for corressponding parameter of a internal oligo less than (Lt) or greater than (Gt) the optimal value.</p></body></html><html><head/><body><p>Вес штрафа для соответствующего параметра внутреннего олиго меньше чем (Lt) или больше чем (Gt), что является оптимальным значением.</p></body></html><html><head/><body><p>Penalty weight for an internal oligo parameter different from predefined optimum value.</p></body></html><html><head/><body><p>Вес штрафа для параметра внутреннего олиго отличается от оптимального предопределенного значения.</p></body></html><html><head/><body><p>In this section user can specify if primer product must overlap exon-exon junction or span intron. This only applies when designing primers for a cDNA (mRNA) sequence with annotated exons.</p></body></html><html><head/><body><p>In this section user can specify if primer product must overlap exon-exon junction or span intron. This only applies when designing primers for a cDNA (mRNA) sequence with annotated exons.</p></body></html><html><head/><body><p>This feature allows to specify if primer product must span intron-exon boundaries.</p><p>Checking this option will result in ignoring <span style=" font-style:italic;">Excluded</span> and <span style=" font-style:italic;">Target Regions</span> from Main section.</p></body></html><html><head/><body><p>Эта опция позволяет указать если праймер должен охватывать интрон-экзон границы.</p></body></html><html><head/><body><p>The name of the annotation which defines the exons in the mRNA sequence.</p></body></html><html><head/><body><p>Имя экзон аннотации.</p></body></html><html><head/><body><p>If the range is set, primer search will be restricted to selected exons. For example:</p><p><span style=" font-weight:600;">1-5</span></p><p>If the range is larger than actual exon range or the starting exon number exceeds number of exons, error message is shown. </p></body></html><html><head/><body><p>If the range is set, primer search will be restricted to selected exons. For example:</p><p><span style=" font-weight:600;">1-5</span></p><p>If the range is larger than actual exon range or the starting exon number exceeds number of exons, error message is shown. </p></body></html><html><head/><body><p>This number of found primer pairs wil bel queried to check if they fullfill the requirements for RTPCR.</p></body></html><html><head/><body><p>Максимальное число пар для запроса.</p></body></html><html><head/><body><p>In this section user can specify sequence quality of target sequence and related parameters.</p></body></html><html><head/><body><p>In this section user can specify sequence quality of target sequence and related parameters.</p></body></html><html><head/><body><p>A list of space separated integers. There must be exactly one integer for each base in the Source Sequence if this argument is non-empty. High numbers indicate high confidence in the base call at that position and low numbers indicate low confidence in the base call at that position.</p></body></html><html><head/><body><p>Список разделенных пробелами целых чисел.</p></body></html><html><head/><body><p>The minimum sequence quality allowed within a primer.</p></body></html><html><head/><body><p>Минимальное качество последовательности.</p></body></html><html><head/><body><p>The minimum sequence quality allowed within the 3' pentamer of a primer.</p></body></html><html><head/><body><p>Минимальное 3'качество последовательности.</p></body></html><html><head/><body><p>The minimum legal sequence quality.</p></body></html><html><head/><body><p>Минимум интервала качества последовательности.</p></body></html><html><head/><body><p>The maximum legal sequence quality.</p></body></html><html><head/><body><p>Максимум интервала качества последовательности.</p></body></html>Result SettingsПараметры результатовQObjectPrimerПраймерPCR primer designКонструкция PCR праймераExcluded regionsИсключенные регионыTargetsЦелевые регионыProduct size rangesДиапазоны размера продуктаNumber to returnРезультатов не болееMax repeat misprimingМаксимальный повтор ошибочного праймированияMax template misprimingМаксимальный шаблон ошибочного праймированияMax 3' stabilityМаксимальная 3' стабильностьPair max repeat misprimingПарный максимальный повтор ошибочного праймированияPair max template misprimingПарный максимальный шаблон ошибочного праймированияU2::FindExonRegionsTaskFailed to search for exon annotations. The sequence %1 doesn't have any related annotations.Failed to search for exon annotations. The sequence %1 doesn't have any related annotations.U2::GTestIllegal TARGET value: %1Illegal TARGET value: %1Illegal PRIMER_DEFAULT_PRODUCT value: %1Illegal PRIMER_DEFAULT_PRODUCT value: %1Illegal PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION value: %1Illegal PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION value: %1Illegal INCLUDED_REGION value: %1Illegal INCLUDED_REGION value: %1Illegal EXCLUDED_REGION value: %1Illegal EXCLUDED_REGION value: %1Unrecognized PRIMER_TASKUnrecognized PRIMER_TASKContradiction in primer_task definitionContradiction in primer_task definitionMissing SEQUENCE tagMissing SEQUENCE tagError in sequence quality dataError in sequence quality dataSequence quality data missingSequence quality data missingPRIMER_PAIRS_NUMBER is incorrect. Expected:%2, but Actual:%3PRIMER_PAIRS_NUMBER is incorrect. Expected:%2, but Actual:%3%1 is incorrect. Expected:%2,%3, but Actual:NULL%1 is incorrect. Expected:%2,%3, but Actual:NULL%1 is incorrect. Expected:NULL, but Actual:%2,%3%1 is incorrect. Expected:NULL, but Actual:%2,%3%1 is incorrect. Expected:%2,%3, but Actual:%4,%5%1 is incorrect. Expected:%2,%3, but Actual:%4,%5%1 is incorrect. Expected:%2, but Actual:%3%1 is incorrect. Expected:%2, but Actual:%3U2::Primer3ADVContextPrimer3...Primer3: Подбор праймеров...ErrorОшибкаCannot create an annotation object. Please check settingsНевозможно создать аннотацию. Проверьте настройкиU2::Primer3DialogNONEнетHUMANHUMANRODENT_AND_SIMPLERODENT_AND_SIMPLERODENTRODENTDROSOPHILADROSOPHILAStart Codon PositionПозиция стартового кодонаProduct Min TmМинимальная Tm продуктаProduct Opt TmОптимальная Tm продуктаProduct Max TmМаксимальная Tm продуктаOpt GC%Оптимальный GC%Inside PenaltyВнутренний штрафInternal Oligo Opt TmОптимальная внутренняя Oligo TmExcluded RegionsИсключаемые регионыInternal Oligo Excluded RegionsВнутренние исключенные Oligo регионыTargetsЦелевые регионыIncluded region is too small for current product size rangesВключенный регион слишком малений для текущих диапазонов размера продуктаProduct Size RangesДиапазоны размеров продуктаSequence QualityКачество последовательностиSequence quality list length must be equal to the sequence lengthДлина списка качества последовательности должна быть равна длине последовательностиCan't pick hyb oligo and only one primerCan't pick hyb oligo and only one primerNothing to pickNothing to pickThe field '%1' has invalid valueThe field '%1' has invalid valueCannot create an annotation object. Please check settings.Save primer settingsСохранить настройки праймераLoad settingsЗагрузить настройкиCan not load settings file: invalid format.Невозможно загрузить настройки: неверный формат.U2::Primer3PluginPrimer3Primer3Integrated tool for PCR primers design.Инструмент для дизайна праймеров.U2::Primer3TaskPick primers taskВыбор праймеровU2::Primer3ToAnnotationsTaskSearch primers to annotationsSearch primers to annotationsFailed to find any exon annotations associated with the sequence %1.Make sure the provided sequence is cDNA and has exonic structure annotatedFailed to find any exon annotations associated with the sequence %1.Make sure the provided sequence is cDNA and has exonic structure annotatedThe first exon from the selected range [%1,%2] is larger the number of exons (%2). Please set correct exon range.The first exon from the selected range [%1,%2] is larger the number of exons (%2). Please set correct exon range.The the selected exon range [%1,%2] is larger the number of exons (%2). Please set correct exon range.The the selected exon range [%1,%2] is larger the number of exons (%2). Please set correct exon range.U2::QDPrimerActor%1 invalid input. Excluded regions.%1 invalid input. Excluded regions.%1 invalid input. Targets.%1 invalid input. Targets.%1 invalid input. Product size ranges.%1 invalid input. Product size ranges.