ConstructMoleculeDialog Construct Molecule Construct Molecule Construction Construction Fragments available from active project Fragments available from active project Add selected fragments to new molecule contents Add selected fragments to new molecule contents Add Add From Project From Project Add all available fragments to molecule contets Add all available fragments to molecule contets Add All Add All New molecule contents: New molecule contents: New molecule contents New molecule contents 5' 5' Fragment Fragment 3' 3' Inverted Inverted Move selected fragment up Move selected fragment up Up Up Move selected fragment down Move selected fragment down Down Down Remove selected fragment from new molecule Remove selected fragment from new molecule Remove Remove Clear molecule contents Clear molecule contents Clear All Clear All Annotate fragments in new molecule Annotate fragments in new molecule Force "blunt" and omit all overhangs Force "blunt" and omit all overhangs Make circular Make circular Setting this option checked will result in ignoring overhangs while constructing new molecule. Setting this option checked will result in ignoring overhangs while constructing new molecule. Available fragments (core length - length without sticky ends): Available fragments (core length - length without sticky ends): Edit Edit Circulirize result molecule Circulirize result molecule Output Output Path to file: Path to file: ... ... Open view for new molecule Open view for new molecule Save immediately Save immediately CreateFragmentDialog Create DNA Fragment Create DNA Fragment Fragment Options Fragment Options Include Left Overhang Include Left Overhang Direct Direct Reverse-complement Reverse-complement Include Right Overhang Include Right Overhang Output Output DigestSequenceDialog Digest Sequence into Fragments Digest Sequence into Fragments Restriction Sites Restriction Sites Target Sequence: Target Sequence: <sequence name> <sequence name> Available enzymes: Available enzymes: Selected enzymes: Selected enzymes: Add --> Add --> Remove Remove Clear Selection Clear Selection Add All --> Add All --> Conserved Annotations Conserved Annotations List of conserved annotations: List of conserved annotations: Conserved annotations can not be "distrupted" when cutting occurs Conserved annotations can not be "distrupted" when cutting occurs Add Add Remove All Remove All Output Output Use this option to treat the molecule as circular Use this option to treat the molecule as circular Circular molecule Circular molecule <hint label> <hint label> EditFragmentDialog Edit Molecule Fragment Edit Molecule Fragment Overhang Overhang Custom overhang Custom overhang Type Type 5'-3' 5'-3' 3'-5' 3'-5' Right End Right End Preview: Preview: Reset Reset Left End Left End Blunt Blunt EnzymesSelectorDialog Select Enzymes Select Enzymes EnzymesSelectorWidget Select enzymes Select enzymes Filter by name: Filter by name: Name Name Accession Accession Type Type Sequence Sequence Organizm / Details Organizm / Details Selected enzymes: Selected enzymes: Load enzymes database Load enzymes database Open enzymes Open enzymes Export enzymes Export enzymes Export current selection as new database Export current selection as new database Select all enzymes in database Select all enzymes in database Hint: selection file should contain enzymes' names separtated by whitespaces or commas Hint: selection file should contain enzymes' names separtated by whitespaces or commas Save selected enzymes list to a text file Save selected enzymes list to a text file Select All Select All <!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd"> <html><head><meta name="qrichtext" content="1" /><style type="text/css"> p, li { white-space: pre-wrap; } </style></head><body style=" font-family:'Lucida Grande'; font-size:13pt; font-weight:400; font-style:normal;"> <p style="-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-family:'MS Shell Dlg 2'; font-size:8pt;"><br /></p></body></html> <!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0//EN" "http://www.w3.org/TR/REC-html40/strict.dtd"> <html><head><meta name="qrichtext" content="1" /><style type="text/css"> p, li { white-space: pre-wrap; } </style></head><body style=" font-family:'Lucida Grande'; font-size:13pt; font-weight:400; font-style:normal;"> <p style="-qt-paragraph-type:empty; margin-top:0px; margin-bottom:0px; margin-left:0px; margin-right:0px; -qt-block-indent:0; text-indent:0px; font-family:'MS Shell Dlg 2'; font-size:8pt;"><br /></p></body></html> Empty selection Empty selection Select None Select None Select enzymes by length of recognition sequence Select enzymes by length of recognition sequence Select by length Select by length Invert current selection Invert current selection Invert selection Invert selection Load selection Load selection Save selection Save selection REBASE Info REBASE Info FindEnzymesDialog Find Restriction Sites Find Restriction Sites Include only enzymes that cut from 'Minimum Hits' to 'Maximum Hits' times Include only enzymes that cut from 'Minimum Hits' to 'Maximum Hits' times Filter by number of results: Filter by number of results: Minimum hits: Minimum hits: Maximum hits: Maximum hits: Search in: Search in: <status label> <status label> QObject The restrictions sites are being updated. Please wait The restrictions sites are being updated. Please wait Hint: there are no available enzymes. Use "Analyze->Find Restrictions Sites" feature to find them. Hint: there are no available enzymes. Use "Analyze->Find Restrictions Sites" feature to find them. U2::ConstructMoleculeDialog No fragments are selected! Please construct molecule from available fragments. No fragments are selected! Please construct molecule from available fragments. Set new molecule file name Set new molecule file name core length core length Blunt Blunt Fwd Fwd OK OK Cancel Cancel Rev Rev Left end Left end Right end Right end yes yes no no Make fragment reverse complement Make fragment reverse complement U2::CreateFragmentDialog OK OK Cancel Cancel Left end contains unsupported symbols! Left end contains unsupported symbols! Right end contains unsupported symbols! Right end contains unsupported symbols! Invalid fragment region! Choose another region. Invalid fragment region! Choose another region. Error Error Cannot create an annotation object. Please check settings Cannot create an annotation object. Please check settings U2::DigestSequenceDialog OK OK Cancel Cancel No enzymes are selected! Please select enzymes. No enzymes are selected! Please select enzymes. Cannot load enzymes library Cannot load enzymes library Error Error Cannot create an annotation object. Please check settings Cannot create an annotation object. Please check settings : %1 cut(s) : %1 cut(s) Auto-annotations update task is NULL. Auto-annotations update task is NULL. Select annotations Select annotations U2::DigestSequenceTask Can't use restriction site %1 for digestion, cleavage site is unknown Can't use restriction site %1 for digestion, cleavage site is unknown Unable to digest into fragments: intersecting restriction sites %1 (%2..%3) and %4 (%5..%6) Unable to digest into fragments: intersecting restriction sites %1 (%2..%3) and %4 (%5..%6) circular circular linear linear <h3><br>Digest into fragments %1 (%2)</h3> <h3><br>Digest into fragments %1 (%2)</h3> <br>Generated %1 fragments. <br>Generated %1 fragments. <br><br>    %1:    From %3 (%2) To %5 (%4) - %6 bp <br><br>    %1:    From %3 (%2) To %5 (%4) - %6 bp Conserved annotation %1 (%2) is disrupted by the digestion. Try changing the restriction sites. Conserved annotation %1 (%2) is disrupted by the digestion. Try changing the restriction sites. U2::EditFragmentDialog OK OK Cancel Cancel Left overhang is empty. Please enter the overhang or set blunt left end. Left overhang is empty. Please enter the overhang or set blunt left end. Invalid left overhang: unsupported alphabet! Invalid left overhang: unsupported alphabet! Right overhang is empty. Please enter the overhang or set blunt right end. Right overhang is empty. Please enter the overhang or set blunt right end. Invalid right overhang: unsupported alphabet! Invalid right overhang: unsupported alphabet! (INVERTED) (INVERTED) Fragment of %1%2<br> Fragment of %1%2<br> <tr> <td align="center"> 5' </td><td></td> <td align="center"> 3' </td> </tr> <tr> <td align="center"> 5' </td><td></td> <td align="center"> 3' </td> </tr> <tr> <td align="center"> 3' </td><td></td> <td align="center"> 5' </td> </tr> <tr> <td align="center"> 3' </td><td></td> <td align="center"> 5' </td> </tr> U2::EnzymesADVContext Find restriction sites... Find restriction sites... Create PCR product... Create PCR product... Cloning Cloning U2::EnzymesIO Bairoch format Bairoch format Unsupported URI type Unsupported URI type Unsupported enzymes file format Unsupported enzymes file format The enzyme '%1' has unknown sequence The enzyme '%1' has unknown sequence No enzyme alphabet: '%1', sequence '%2' No enzyme alphabet: '%1', sequence '%2' Non-nucleic enzyme alphabet: '%1', alphabet: %2, sequence '%3' Non-nucleic enzyme alphabet: '%1', alphabet: %2, sequence '%3' Line is too long: %1 Line is too long: %1 Restriction enzymes: Illegal cut pos: %1, line %2 Restriction enzymes: Illegal cut pos: %1, line %2 U2::EnzymesPlugin Finds and annotates restriction sites on a DNA sequence. Finds and annotates restriction sites on a DNA sequence. Create fragment... Create fragment... Digest into fragments... Digest into fragments... Construct molecule... Construct molecule... There is no active sequence object. To start partition open sequence document. There is no active sequence object. To start partition open sequence document. Can not digest into fragments non-nucleic sequence. Can not digest into fragments non-nucleic sequence. There is no active sequence object. To create fragment open sequence document. There is no active sequence object. To create fragment open sequence document. The sequence doesn't have nucleic alphabet, it can not be used in cloning. The sequence doesn't have nucleic alphabet, it can not be used in cloning. There is no active project. To start ligation create a project or open an existing. There is no active project. To start ligation create a project or open an existing. Restriction analysis Restriction analysis U2::EnzymesSelectorDialog OK OK Cancel Cancel U2::EnzymesSelectorWidget File not exists: %1 File not exists: %1 Error Error New enzymes database has been saved. New enzymes database has been saved. Do you want to work with new database? Do you want to work with new database? Select enzyme database file Select enzyme database file Minimum length Minimum length Enter minimum length of recognition sites Enter minimum length of recognition sites Save selection Save selection Can not save empty selection! Can not save empty selection! Select enzymes selection Select enzymes selection Failed to open %1 for writing Failed to open %1 for writing Error! Error! No enzyme selected! No enzyme selected! Selected enzyme has no ID! Selected enzyme has no ID! Selection files Selection files Load selection Load selection Failed to open selection file %1 Failed to open selection file %1 Enzymes selection is empty! Enzymes selection is empty! Failed to load %1 from selection. Failed to load %1 from selection. U2::FindEnzymesAutoAnnotationUpdater Restriction Sites Restriction Sites U2::FindEnzymesDialog OK OK Cancel Cancel Total number of enzymes: %1, selected %2 Total number of enzymes: %1, selected %2 Invalid 'Search' region! Invalid 'Search' region! <html><body align="center">No enzymes are selected! Do you want to turn off <br>enzymes annotations highlighting?</body></html> <html><body align="center">No enzymes are selected! Do you want to turn off <br>enzymes annotations highlighting?</body></html> Error! Error! Minimum hit value must be lesser or equal then maximum! Minimum hit value must be lesser or equal then maximum! U2::FindEnzymesTask Find Enzymes Find Enzymes Alphabet is not nucleic. Alphabet is not nucleic. Number of results exceed %1, stopping Number of results exceed %1, stopping Found %1 restriction sites Number of results exceed %1, stopping U2::FindEnzymesToAnnotationsTask Find and store enzymes Find and store enzymes No enzymes selected. No enzymes selected. Annotation table does not exist Annotation table does not exist Annotation table is read-only Annotation table is read-only U2::FindSingleEnzymeTask Find enzyme '%1' Find enzyme '%1' Alphabet is not nucleic. Alphabet is not nucleic. Find enzyme '%1' parallel Find enzyme '%1' parallel No enzyme alphabet No enzyme alphabet Non-nucleic enzyme alphabet: %1, enzyme: %2, skipping.. Non-nucleic enzyme alphabet: %1, enzyme: %2, skipping.. Failed to get sequence alphabet Failed to get sequence alphabet U2::LigateFragmentsTask Fragments %1 and %2 are inconsistent. Blunt and sticky ends incompatibility Fragments %1 and %2 are inconsistent. Blunt and sticky ends incompatibility Right overhang from %1 and left overhang from %2 are inconsistent. Right overhang from %1 and left overhang from %2 are inconsistent. Unknown DNA alphabet in fragment %1 of %2 Unknown DNA alphabet in fragment %1 of %2 Add constructed molecule Add constructed molecule U2::LoadEnzymeFileTask Load enzymes from %1 Load enzymes from %1 U2::QDEnzymesActor Find enzymes Find enzymes Enzymes query Enzymes query Restriction Sites Restriction Sites Finds restriction cut sites in supplied DNA sequence. Finds restriction cut sites in supplied DNA sequence. Enzymes Enzymes Restriction enzymes used to recognize the restriction sites. Restriction enzymes used to recognize the restriction sites. Circular Circular If <i>True</i> considers the sequence circular. That allows to search for restriction sites between the end and the beginning of the sequence. If <i>True</i> considers the sequence circular. That allows to search for restriction sites between the end and the beginning of the sequence. U2::SaveEnzymeFileTask Save enzymes to %1 Save enzymes to %1