EditPrimerDialog Create New Primer Create New Primer Name Name New Primer New Primer ExportPrimersDialog Export Primers Export Primers Export to: Export to: Format: Format: File path: File path: ... ... Database: Database: Destination folder: Destination folder: Connect Connect Global Error Error ImportPrimersDialog Import Primers Import Primers Import from: Import from: Connect to database Connect to database Files: Files: Add file(s) Add file(s) Remove Remove Objects: Objects: Add object(s) Add object(s) InSilicoPcrOptionPanelWidget Form Form Maximum product Maximum product bp bp 3' perfect match 3' perfect match Extract annotations Extract annotations <a href="details">Show primers details</a> <a href="details">Show primers details</a> Find product(s) Find product(s) Region Region Length Length Ta Ta Extract product(s) Extract product(s) Info: choose a nucleic sequence for running In Silico PCR Info: choose a nucleic sequence for running In Silico PCR PrimerGroupBox Form Form Reverse-complement sequence Reverse-complement sequence Choose a primer from the primer library Choose a primer from the primer library Mismatches Mismatches bp bp PrimerLibrarySelector Choose Primer Choose Primer PrimerLibraryWidget Form Form PrimersDetailsDialog Primers Details Primers Details QObject Local file Local file Shared database Shared database Local file(s) Local file(s) U2::EditPrimerDialog Edit Primer Edit Primer U2::ExportPrimersDialog Export to Export to U2::ExportPrimersToDatabaseTask Export primers Export primers Unexpected objects count Unexpected objects count U2::ExportPrimersToLocalFileTask Export primers Export primers U2::ExtractProductTask Extract PCR product Extract PCR product U2::ExtractProductWrapperTask Extract PCR product and open document Extract PCR product and open document U2::FindPrimersTask FindPrimersTask FindPrimersTask Correct primers pairs Correct primers pairs No IO adapter found for URL: %1 No IO adapter found for URL: %1 U2::ImportPrimerFromObjectTask Import primer from the shared database object: %1 Import primer from the shared database object: %1 A non-sequence object was provided A non-sequence object was provided The sequence has an unsupported alphabet: only standard nucleotide alphabet is supported The sequence has an unsupported alphabet: only standard nucleotide alphabet is supported The sequence has an unsupported alphabet: only nucleotide alphabet is supported The sequence has an unsupported alphabet: only nucleotide alphabet is supported Can't convert a sequence to primer: the sequence is too long Can't convert a sequence to primer: the sequence is too long The primer sequence contains non-ACGT symbols The primer sequence contains non-ACGT symbols Without document Without document cancelled cancelled error error success success U2::ImportPrimersDialog Import Import Cancel Cancel Select primers to import Select primers to import U2::ImportPrimersFromFileTask Import primers from local file: %1 Import primers from local file: %1 Internal error: can't load the file '%1' Internal error: can't load the file '%1' Document wasn't loaded Document wasn't loaded There are no sequence objects in the file '%1' to convert them to primers There are no sequence objects in the file '%1' to convert them to primers cancelled cancelled error error there are no applicable sequences there are no applicable sequences success success U2::ImportPrimersFromFolderTask Import primers from the shared database folder: %1 Import primers from the shared database folder: %1 U2::ImportPrimersMultiTask Import primers Import primers U2::InSilicoPcrOPWidgetFactory In Silico PCR In Silico PCR U2::InSilicoPcrOptionPanelWidget Forward primer Forward primer Reverse primer Reverse primer Settings Settings Inner All intersected None Show primers details Show primers details Find product(s) Find product(s) Warning: Warning: Find product(s) anyway Find product(s) anyway Extract In Silico PCR products Extract In Silico PCR products U2::InSilicoPcrProductsTable N/A U2::InSilicoPcrTask In Silico PCR In Silico PCR Forward primers found: %1 Forward primers found: %1 Reverse primers found: %1 Reverse primers found: %1 Products found: %1 Products found: %1 Primers details: Primers details: Products found: %1. The detailed information about primers is not available as primers or sequence contain a character from the Extended DNA alphabet. U2::InSilicoPcrWorkflowTask In silico PCR workflow task U2::LocalWorkflow::FindPrimerPairsPromter unset unset Find correct pairs over primers from "%1". Find correct pairs over primers from "%1". U2::LocalWorkflow::FindPrimerPairsWorker No correct primers pairs found No correct primers pairs found Input sequences Input sequences Set of primers, which must be tested. Set of primers, which must be tested. Find correct primer pairs Find correct primer pairs Find correct primer pairs, which consist of valid primers without dimers. Find correct primer pairs, which consist of valid primers without dimers. Output report file Output report file Path to the report output file. Path to the report output file. Report file Report file Forward primer name Forward primer name Reverse primer name Reverse primer name Forward Tm Forward Tm Reverse Tm Reverse Tm U2::LocalWorkflow::InSilicoPcrPrompter unset unset Simulates PCR for the sequences from %1 and primer pairs from %2. Simulates PCR for the sequences from %1 and primer pairs from %2. U2::LocalWorkflow::InSilicoPcrReportTask Generate In Silico PCR report Generate In Silico PCR report Sequence name Sequence name Products count table Products count table Primer pair details Primer pair details U2::LocalWorkflow::InSilicoPcrWorker Input sequence Input sequence Input sequence. Input sequence. PCR product PCR product PCR product sequence. PCR product sequence. Primers URL Primers URL A URL to the input file with primer pairs. A URL to the input file with primer pairs. Report URL Report URL A URL to the output file with the PCR report. A URL to the output file with the PCR report. Mismatches Mismatches Number of allowed mismatches. Number of allowed mismatches. Min perfect match Min perfect match Number of bases that match exactly on 3' end of primers. Number of bases that match exactly on 3' end of primers. Max product size Max product size Maximum size of amplified region. Maximum size of amplified region. Extract annotations Extract annotations Extract annotations within a product region. Inner All intersected None In Silico PCR In Silico PCR Simulates PCR for input sequences and primer pairs. Creates the table with the PCR statistics. Simulates PCR for input sequences and primer pairs. Creates the table with the PCR statistics. Can not read the primers file: Can not read the primers file: Can't read the file: Can't read the file: No primer sequences in the file: No primer sequences in the file: There is the odd number of primers in the file: There is the odd number of primers int the file: There is the odd number of primers int the file: Primer sequence is too long: %1. The pair is skipped Primer sequence is too long: %1. The pair is skipped Wrong sequence objects count Wrong annotations objects count The sequence is too long: The sequence is too long: Multiple In Silico PCR Multiple In Silico PCR U2::LocalWorkflow::PrimersGrouperPromter unset unset Read primers from %1 by pairs. Select groups of primer pairs, which can be simultaneously used in one reaction tube. Save result to %2. Read primers from %1 by pairs. Select groups of primer pairs, which can be simultaneously used in one reaction tube. Save result to %2. U2::LocalWorkflow::PrimersGrouperWorker No valid groups found No valid groups found Primer pairs Primer pairs Pairs of primers, which must be grouped. Pairs of primers, which must be grouped. Group Primer Pairs Group Primer Pairs Select groups of primer pairs, which can be simultaneously used in one reaction tube.The primers must be supplied in the following order: pair1_direct_primer, pair1_reverse_primer, pair2_direct_primer, pair2_reverse_primer, etc. Select groups of primer pairs, which can be simultaneously used in one reaction tube.The primers must be supplied in the following order: pair1_direct_primer, pair1_reverse_primer, pair2_direct_primer, pair2_reverse_primer, etc. Output report file Output report file Path to the report output file. Path to the report output file. Report file Report file Group name Group name Forward primer name Forward primer name Forward primer sequence Forward primer sequence Reverse primer name Reverse primer name Reverse primer sequence Reverse primer sequence U2::PcrPlugin In silico PCR In silico PCR Primer library Primer library U2::PrimerGroupBox N/A -mer -mer Tm = Tm = U2::PrimerGrouperTask Primer grouper task Primer grouper task Group %1 Group %1 No IO adapter found for URL: %1 No IO adapter found for URL: %1 U2::PrimerLibraryMdiWindow Primer Library Primer Library U2::PrimerLibraryModel Name Name GC-content (%) GC-content (%) Length (bp) Length (bp) Sequence Sequence Incorrect primer number Incorrect primer number N/A U2::PrimerLibrarySelector Choose Choose U2::PrimerLibraryTable Your primer library is empty. Use "Tools -> Primer -> Primer Library" for managing the library. Your primer library is empty. Use "Tools -> Primer -> Primer Library" for managing the library. U2::PrimerLibraryWidget New primer New primer Edit primer Edit primer Remove primer(s) Remove primer(s) Import primer(s) Import primer(s) Export primer(s) Export primer(s) U2::PrimerStatistics The primers contain a character from the Extended DNA alphabet. The forward primer contains a character from the Extended DNA alphabet. The reverse primer contains a character from the Extended DNA alphabet. low GC-content low GC-content high GC-content high GC-content low melting temperature low melting temperature high melting temperature high melting temperature low GC clamp low GC clamp high base runs value high base runs value forward primer has %1. forward primer has %1. reverse primer has %1. reverse primer has %1. Criteria Criteria Valid Values Valid Values Forward Forward Reverse Reverse