1) Suggested revision: For this example, we can avoid using the Excluded regions box by simply specifying a range for both the forward and reverse primers at the top of the Primer-BLAST window. The main advantage of the Excluded region box is if you want to exclude more than one set of regions. 2) Although Primer-BLAST doesn't have a mechanism for saving the primer results, you can copy the table and past it into a document eg. LibreOffice Writer or Seamonkey Composer.